(Circular) (Six-base) MAPSORT of: pFN21AB8477.seq Check: 5266 from: 1 to: 7447 pFN21AB8477 7447 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2946 3941 2946 Size: 995 6452 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5257 278 Size: 53 83 186 936 3721 2468 Fragments arranged by size: 3721 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2178 2880 3481 4790 1937 Size: 241 702 601 1309 4594 Fragments arranged by size: 4594 1309 702 601 241 Acc65I G'GTAC_C Cuts at: 4768 4768 Size: 7447 AclI AA'CG_TT Cuts at: 1563 5574 5947 1563 Size: 4011 373 3063 Fragments arranged by size: 4011 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2142 2289 2571 2826 2870 3326 3537 3930 Size: 510 147 282 255 44 456 211 393 Cuts at: 3930 4192 4538 4757 5510 6894 7162 1632 Size: 262 346 219 753 1384 268 1917 Fragments arranged by size: 1917 1384 753 510 456 393 346 282 268 262 255 219 211 147 44 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7250 AflIII A'CryG_T Cuts at: 1203 2905 6346 7323 1203 Size: 1702 3441 977 1327 Fragments arranged by size: 3441 1702 1327 977 AgeI A'CCGG_T Cuts at: 6278 6278 Size: 7447 AhdI GACnn_n'nnGTC Cuts at: 3773 6176 3773 Size: 2403 5044 AleI CACnn'nnGTG Cuts at: 1166 4130 1166 Size: 2964 4483 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3528 3747 1184 Size: 2344 219 4884 Fragments arranged by size: 4884 2344 219 AlwNI CAG_nnn'CTG Cuts at: 2717 2757 3544 4613 5190 6762 2717 Size: 40 787 1069 577 1572 3402 Fragments arranged by size: 3402 1572 1069 787 577 40 ApaLI G'TGCA_C Cuts at: 4000 5504 6660 4000 Size: 1504 1156 4787 Fragments arranged by size: 4787 1504 1156 ApoI r'AATT_y Cuts at: 1493 4135 4339 4405 4627 4756 4982 5134 7243 Size: 2642 204 66 222 129 226 152 2109 Cuts at: 7243 1493 Size: 1697 Fragments arranged by size: 2642 2109 1697 226 222 204 152 129 66 AseI AT'TA_AT Cuts at: 160 1032 6001 160 Size: 872 4969 1606 Fragments arranged by size: 4969 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7447 AvaI C'yCGr_G Cuts at: 1870 1943 3803 4386 4772 1870 Size: 73 1860 583 386 4545 Fragments arranged by size: 4545 1860 583 386 73 BamHI G'GATC_C Cuts at: 4777 5144 4777 Size: 367 7080 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4556 4768 6223 618 Size: 325 217 627 2769 212 1455 1842 Fragments arranged by size: 2769 1842 1455 627 325 217 212 BanII G_rGCy'C Cuts at: 1391 3407 4766 1391 Size: 2016 1359 4072 Fragments arranged by size: 4072 2016 1359 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3332 4664 928 Size: 971 1433 1332 3711 Fragments arranged by size: 3711 1433 1332 971 BbvCI CC'TCA_GC Cuts at: 3635 3635 Size: 7447 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5638 5672 1903 Size: 34 3701 34 3678 Fragments arranged by size: 3701 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5339 6549 5339 Size: 1210 6237 BclI T'GATC_A Cuts at: 1548 2479 1548 Size: 931 6516 BfrBI ATG'CAT Cuts at: 2240 2240 Size: 7447 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6058 136 Size: 107 122 71 631 605 4386 1525 Fragments arranged by size: 4386 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3062 7442 3062 Size: 4380 3067 BlpI GC'TnA_GC Cuts at: 4864 4864 Size: 7447 Bme1580I G_kGCm'C Cuts at: 1790 4004 4561 5508 6664 1790 Size: 2214 557 947 1156 2573 Fragments arranged by size: 2573 2214 1156 947 557 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7447 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3123 3610 3790 Size: 416 53 83 399 24 1706 487 180 Cuts at: 3790 4535 4697 5178 6136 442 Size: 745 162 481 958 1753 Fragments arranged by size: 1753 1706 958 745 487 481 416 399 180 162 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7447 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2875 4412 2875 Size: 1537 5910 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2241 2474 4280 4424 6107 1239 Size: 1002 233 1806 144 1683 2579 Fragments arranged by size: 2579 1806 1683 1002 233 144 Bpu10I CC'TnA_GC Cuts at: 3470 3635 3470 Size: 165 7282 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2601 2639 4477 5572 6437 6735 2601 Size: 38 1838 1095 865 298 3313 Fragments arranged by size: 3313 1838 1095 865 298 38 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4453 6110 882 Size: 617 2954 1657 2219 Fragments arranged by size: 2954 2219 1657 617 BsaAI yAC'GTr Cuts at: 493 1204 5238 493 Size: 711 4034 2702 Fragments arranged by size: 4034 2702 711 BsaBI GATnn'nnATC Cuts at: 2376 7441 2376 Size: 5065 2382 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5254 5636 6271 275 Size: 53 83 186 936 3721 382 635 1451 Fragments arranged by size: 3721 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5880 6278 6552 6699 7110 5880 Size: 398 274 147 411 6217 Fragments arranged by size: 6217 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2203 2233 1178 Size: 30 165 30 800 30 6392 Fragments arranged by size: 6392 800 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 3404 3863 1186 Size: 1285 933 459 4770 Fragments arranged by size: 4770 1285 933 459 BseYI C'CCAG_C Cuts at: 3268 4070 6650 7146 3268 Size: 802 2580 496 3569 Fragments arranged by size: 3569 2580 802 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2966 3592 1163 Size: 573 1230 626 5018 Fragments arranged by size: 5018 1230 626 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4897 5658 6686 1540 Size: 275 186 2896 761 1028 2301 Fragments arranged by size: 2896 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3407 4004 5508 5593 6664 3407 Size: 597 1504 85 1071 4190 Fragments arranged by size: 4190 1504 1071 597 85 BsmI GAATG_Cn' Cuts at: 2026 4958 5051 2026 Size: 2932 93 4422 Fragments arranged by size: 4422 2932 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2542 2542 Size: 7447 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3407 4004 4561 4766 5508 5593 6664 Size: 399 1617 597 557 205 742 85 1071 Cuts at: 6664 1391 Size: 2174 Fragments arranged by size: 2174 1617 1071 742 597 557 399 205 85 BspEI T'CCGG_A Cuts at: 7110 7110 Size: 7447 BspHI T'CATG_A Cuts at: 2058 3312 5336 7066 2058 Size: 1254 2024 1730 2439 Fragments arranged by size: 2439 2024 1730 1254 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2946 3941 4802 844 Size: 2102 995 861 3489 Fragments arranged by size: 3489 2102 995 861 BsrBI CCG'CTC Cuts at: 1394 1823 3255 5334 1394 Size: 429 1432 2079 3507 Fragments arranged by size: 3507 2079 1432 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2541 5942 6116 65 Size: 1183 1293 3401 174 1396 Fragments arranged by size: 3401 1396 1293 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6091 6278 1700 Size: 110 4281 187 2869 Fragments arranged by size: 4281 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7447 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7447 BssSI C'ACGA_G Cuts at: 2984 5507 6519 2984 Size: 2523 1012 3912 Fragments arranged by size: 3912 2523 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2246 794 Size: 1452 5995 BstBI TT'CG_AA Cuts at: 2530 6251 2530 Size: 3721 3726 BstEII G'GTnAC_C Cuts at: 1184 4063 1184 Size: 2879 4568 BstXI CCAn_nnnn'nTGG Cuts at: 3101 4225 4568 3101 Size: 1124 343 5980 Fragments arranged by size: 5980 1124 343 BstYI r'GATC_y Cuts at: 1972 2113 3062 3534 3924 4777 5144 5529 5546 Size: 141 949 472 390 853 367 385 17 Cuts at: 5546 6998 7084 7096 7442 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 949 853 472 390 385 367 346 141 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3433 3433 Size: 7447 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6895 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3449 487 Size: 683 56 2223 4485 Fragments arranged by size: 4485 2223 683 56 BtsI GCAGTG_nn' Cuts at: 3458 3646 4963 5757 5777 3458 Size: 188 1317 794 20 5128 Fragments arranged by size: 5128 1317 794 188 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7447 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7412 DraI TTT'AAA Cuts at: 3190 4626 4751 3190 Size: 1436 125 5886 Fragments arranged by size: 5886 1436 125 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6454 809 Size: 507 5138 1802 Fragments arranged by size: 5138 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4227 4486 4894 Size: 54 1425 50 275 18 2397 259 408 Cuts at: 4894 5783 7412 8 Size: 889 1629 43 Fragments arranged by size: 2397 1629 1425 889 408 275 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4894 1537 Size: 275 3082 4090 Fragments arranged by size: 4090 3082 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2265 2592 2664 2688 2816 2844 3831 3840 Size: 912 327 72 24 128 28 987 9 Cuts at: 3840 3885 4514 5377 1353 Size: 45 629 863 3423 Fragments arranged by size: 3423 987 912 863 629 327 128 72 45 28 24 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6034 6408 6554 387 Size: 1064 138 4445 374 146 1280 Fragments arranged by size: 4445 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3405 3405 Size: 7447 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2241 2289 2474 2571 2826 2870 Size: 393 510 99 48 185 97 255 44 Cuts at: 2870 3326 3537 3930 4192 4280 4424 4538 4757 Size: 456 211 393 262 88 144 114 219 Cuts at: 4757 5510 6107 6894 7162 1239 Size: 753 597 787 268 1524 Fragments arranged by size: 1524 787 753 597 510 456 393 393 268 262 255 219 211 185 144 114 99 97 88 48 44 EcoNI CCTnn'n_nnAGG Cuts at: 3371 4739 3371 Size: 1368 6079 EcoO109I rG'GnC_Cy Cuts at: 3428 3428 Size: 7447 EcoRI G'AATT_C Cuts at: 4135 4756 5134 4135 Size: 621 378 6448 Fragments arranged by size: 6448 621 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2870 3526 3914 4519 2870 Size: 656 388 605 5798 Fragments arranged by size: 5798 656 605 388 FspI TGC'GCA Cuts at: 5953 5953 Size: 7447 HaeII r_GCGC'y Cuts at: 1678 6594 1678 Size: 4916 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2203 2235 2237 Size: 32 135 32 515 32 84 32 2 Cuts at: 2237 2269 2394 2426 3161 3193 3344 3376 3759 Size: 32 125 32 735 32 151 32 383 Cuts at: 3759 3791 6088 6120 6162 6194 1373 Size: 32 2297 32 42 32 2626 Fragments arranged by size: 2626 2297 735 515 383 151 135 125 84 42 32 32 32 32 32 32 32 32 32 32 32 2 HincII GTy'rAC Cuts at: 669 1657 1938 3482 4791 5037 669 Size: 988 281 1544 1309 246 3079 Fragments arranged by size: 3079 1544 1309 988 281 246 HindIII A'AGCT_T Cuts at: 748 2943 748 Size: 2195 5252 HpaI GTT'AAC Cuts at: 5037 5037 Size: 7447 KpnI G_GTAC'C Cuts at: 4772 4772 Size: 7447 MfeI C'AATT_G Cuts at: 2462 5046 2462 Size: 2584 4863 MluI A'CGCG_T Cuts at: 7323 7323 Size: 7447 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2613 3318 4146 6561 6745 1305 Size: 1308 705 828 2415 184 2007 Fragments arranged by size: 2415 2007 1308 828 705 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4229 4488 10 Size: 54 1425 343 2397 259 2969 Fragments arranged by size: 2969 2397 1425 343 259 54 MslI CAynn'nnrTG Cuts at: 518 1166 2342 4130 5405 5764 5923 518 Size: 648 1176 1788 1275 359 159 2042 Fragments arranged by size: 2042 1788 1275 1176 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2230 2494 2574 3131 3529 4427 4863 5540 Size: 624 264 80 557 398 898 436 677 Cuts at: 5540 6688 6933 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 898 677 624 557 436 398 264 245 80 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6895 NdeI CA'TA_TG Cuts at: 387 3100 387 Size: 2713 4734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7447 NotI GC'GGCC_GC Cuts at: 4894 4894 Size: 7447 NsiI A_TGCA'T Cuts at: 2242 2242 Size: 7447 NspI r_CATG'y Cuts at: 1132 1229 2240 2909 4805 6350 1132 Size: 97 1011 669 1896 1545 2229 Fragments arranged by size: 2229 1896 1545 1011 669 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7447 PciI A'CATG_T Cuts at: 2905 6346 2905 Size: 3441 4006 PflMI CCAn_nnn'nTGG Cuts at: 1108 3415 3544 1108 Size: 2307 129 5011 Fragments arranged by size: 5011 2307 129 PfoI T'CCnGG_A Cuts at: 4440 4466 4440 Size: 26 7421 PmeI GTTT'AAAC Cuts at: 4751 4751 Size: 7447 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3491 3523 5516 5548 7029 7061 3491 Size: 32 1993 32 1481 32 3877 Fragments arranged by size: 3877 1993 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3428 3428 Size: 7447 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7447 PsiI TTA'TAA Cuts at: 5017 5017 Size: 7447 PspXI vC'TCGA_Gb Cuts at: 4386 4386 Size: 7447 PstI C_TGCA'G Cuts at: 830 2499 2631 4799 830 Size: 1669 132 2168 3478 Fragments arranged by size: 3478 2168 1669 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7447 PvuII CAG'CTG Cuts at: 2494 2574 3131 4427 2494 Size: 80 557 1296 5514 Fragments arranged by size: 5514 1296 557 80 SacI G_AGCT'C Cuts at: 3407 3407 Size: 7447 SalI G'TCGA_C Cuts at: 1936 3480 4789 1936 Size: 1544 1309 4594 Fragments arranged by size: 4594 1544 1309 SapI GCTCTTCn'nnn_ Cuts at: 2688 2844 2688 Size: 156 7291 SbfI CC_TGCA'GG Cuts at: 4799 4799 Size: 7447 ScaI AGT'ACT Cuts at: 5695 7407 5695 Size: 1712 5735 SexAI A'CCwGG_T Cuts at: 3769 3769 Size: 7447 SfcI C'TryA_G Cuts at: 826 1045 2495 2627 3453 4030 4153 4795 5930 Size: 219 1450 132 826 577 123 642 1135 Cuts at: 5930 6611 6802 826 Size: 681 191 1471 Fragments arranged by size: 1471 1450 1135 826 681 642 577 219 191 132 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7447 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7447 SmaI CCC'GGG Cuts at: 1872 3805 4774 1872 Size: 1933 969 4545 Fragments arranged by size: 4545 1933 969 SmlI C'TyrA_G Cuts at: 820 1017 1943 2616 2654 4386 4456 5551 6452 Size: 197 926 673 38 1732 70 1095 901 Cuts at: 6452 6714 820 Size: 262 1553 Fragments arranged by size: 1732 1553 1095 926 901 673 262 197 70 38 SnaBI TAC'GTA Cuts at: 493 5238 493 Size: 4745 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7447 SphI G_CATG'C Cuts at: 1132 2240 4805 1132 Size: 1108 2565 3774 Fragments arranged by size: 3774 2565 1108 SspI AAT'ATT Cuts at: 5 52 4038 5371 5 Size: 47 3986 1333 2081 Fragments arranged by size: 3986 2081 1333 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7447 StyI C'CwwG_G Cuts at: 513 1065 2691 4886 513 Size: 552 1626 2195 3074 Fragments arranged by size: 3074 2195 1626 552 SwaI ATTT'AAAT Cuts at: 4626 4626 Size: 7447 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3629 3952 4718 4735 5474 5491 5644 Size: 1255 2049 323 766 17 739 17 153 Cuts at: 5644 5829 6274 325 Size: 185 445 1498 Fragments arranged by size: 2049 1498 1255 766 739 445 323 185 153 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5693 7405 96 Size: 274 80 33 51 1443 3716 1712 138 Fragments arranged by size: 3716 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7407 7439 7407 Size: 32 7415 Tth111I GACn'n_nGTC Cuts at: 1316 2215 1316 Size: 899 6548 XbaI T'CTAG_A Cuts at: 4783 4783 Size: 7447 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4448 4448 Size: 7447 XhoI C'TCGA_G Cuts at: 1943 4386 1943 Size: 2443 5004 XmaI C'CCGG_G Cuts at: 1870 3803 4772 1870 Size: 1933 969 4545 Fragments arranged by size: 4545 1933 969 XmnI GAAnn'nnTTC Cuts at: 3506 5576 3506 Size: 2070 5377 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5255 276 Size: 53 83 186 936 3721 2468 Fragments arranged by size: 3721 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI