(Circular) (Six-base) MAPSORT of: pFN21AE0372.seq Check: 201 from: 1 to: 6400 pFN21AE0372 6400 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3396 4210 278 Size: 53 83 186 936 1860 814 2468 Fragments arranged by size: 2468 1860 936 814 186 83 53 AccI GT'mk_AC Cuts at: 1937 2653 3186 3220 3743 1937 Size: 716 533 34 523 4594 Fragments arranged by size: 4594 716 533 523 34 Acc65I G'GTAC_C Cuts at: 3721 3721 Size: 6400 AclI AA'CG_TT Cuts at: 1563 4527 4900 1563 Size: 2964 373 3063 Fragments arranged by size: 3063 2964 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 3192 4463 5847 6115 1632 Size: 462 1098 1271 1384 268 1917 Fragments arranged by size: 1917 1384 1271 1098 462 268 AflII C'TTAA_G Cuts at: 820 1017 3397 820 Size: 197 2380 3823 Fragments arranged by size: 3823 2380 197 AflIII A'CryG_T Cuts at: 1203 2477 3638 5299 6276 1203 Size: 1274 1161 1661 977 1327 Fragments arranged by size: 1661 1327 1274 1161 977 AgeI A'CCGG_T Cuts at: 5231 5231 Size: 6400 AhdI GACnn_n'nnGTC Cuts at: 5129 5129 Size: 6400 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6400 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6400 AlwNI CAG_nnn'CTG Cuts at: 2132 4143 5715 2132 Size: 2011 1572 2817 Fragments arranged by size: 2817 2011 1572 ApaLI G'TGCA_C Cuts at: 4457 5613 4457 Size: 1156 5244 ApoI r'AATT_y Cuts at: 1493 2405 2761 3194 3709 3935 4087 6196 1493 Size: 912 356 433 515 226 152 2109 1697 Fragments arranged by size: 2109 1697 912 515 433 356 226 152 AseI AT'TA_AT Cuts at: 160 1032 2855 4954 160 Size: 872 1823 2099 1606 Fragments arranged by size: 2099 1823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 AvaI C'yCGr_G Cuts at: 1870 1943 3725 1870 Size: 73 1782 4545 Fragments arranged by size: 4545 1782 73 BamHI G'GATC_C Cuts at: 3730 4097 3730 Size: 367 6033 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3721 5176 618 Size: 325 217 627 1934 1455 1842 Fragments arranged by size: 1934 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2084 2474 3719 1391 Size: 693 390 1245 4072 Fragments arranged by size: 4072 1245 693 390 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2008 2802 928 Size: 971 109 794 4526 Fragments arranged by size: 4526 971 794 109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4591 4625 1903 Size: 34 2654 34 3678 Fragments arranged by size: 3678 2654 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4292 5502 4292 Size: 1210 5190 BclI T'GATC_A Cuts at: 1548 2518 2859 1548 Size: 970 341 5089 Fragments arranged by size: 5089 970 341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5011 136 Size: 107 122 71 631 605 3339 1525 Fragments arranged by size: 3339 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2557 6395 2557 Size: 3838 2562 BlpI GC'TnA_GC Cuts at: 3817 3817 Size: 6400 Bme1580I G_kGCm'C Cuts at: 1790 2305 4461 5617 1790 Size: 515 2156 1156 2573 Fragments arranged by size: 2573 2156 1156 515 BmgBI CAC'GTC Cuts at: 1318 3599 1318 Size: 2281 4119 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4131 5089 442 Size: 416 53 83 399 24 2714 958 1753 Fragments arranged by size: 2714 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6400 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2178 3162 2178 Size: 984 5416 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2403 2982 3135 5060 1239 Size: 1164 579 153 1925 2579 Fragments arranged by size: 2579 1925 1164 579 153 Bpu10I CC'TnA_GC Cuts at: 3137 3137 Size: 6400 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2660 4525 5390 5688 2660 Size: 1865 865 298 3372 Fragments arranged by size: 3372 1865 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2380 5063 882 Size: 617 881 2683 2219 Fragments arranged by size: 2683 2219 881 617 BsaAI yAC'GTr Cuts at: 493 1204 2480 4191 493 Size: 711 1276 1711 2702 Fragments arranged by size: 2702 1711 1276 711 BsaBI GATnn'nnATC Cuts at: 6394 6394 Size: 6400 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3393 4207 4589 5224 Size: 53 83 186 936 1860 814 382 635 Cuts at: 5224 275 Size: 1451 Fragments arranged by size: 1860 1451 936 814 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4833 5231 5505 5652 6063 4833 Size: 398 274 147 411 5170 Fragments arranged by size: 5170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3111 3141 3225 3255 1178 Size: 30 165 30 1708 30 84 30 4323 Fragments arranged by size: 4323 1708 165 84 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2510 2685 3138 1186 Size: 1324 175 453 4448 Fragments arranged by size: 4448 1324 453 175 BseYI C'CCAG_C Cuts at: 5603 6099 5603 Size: 496 5904 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5827 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3850 4611 5639 1540 Size: 275 186 1849 761 1028 2301 Fragments arranged by size: 2301 1849 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2084 4461 4546 5617 2084 Size: 2377 85 1071 2867 Fragments arranged by size: 2867 2377 1071 85 BsmI GAATG_Cn' Cuts at: 3182 3911 4004 3182 Size: 729 93 5578 Fragments arranged by size: 5578 729 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2305 2474 3719 4461 4546 5617 Size: 399 294 221 169 1245 742 85 1071 Cuts at: 5617 1391 Size: 2174 Fragments arranged by size: 2174 1245 1071 742 399 294 221 169 85 BspEI T'CCGG_A Cuts at: 6063 6063 Size: 6400 BspHI T'CATG_A Cuts at: 4289 6019 4289 Size: 1730 4670 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3755 844 Size: 2911 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4287 1394 Size: 429 2464 3507 Fragments arranged by size: 3507 2464 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2103 3087 4895 5069 65 Size: 1183 855 984 1808 174 1396 Fragments arranged by size: 1808 1396 1183 984 855 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5044 5231 1700 Size: 110 3234 187 2869 Fragments arranged by size: 3234 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6400 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6400 BssSI C'ACGA_G Cuts at: 4460 5472 4460 Size: 1012 5388 BstAPI GCAn_nnn'nTGC Cuts at: 794 2894 794 Size: 2100 4300 BstBI TT'CG_AA Cuts at: 5204 5204 Size: 6400 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6400 BstYI r'GATC_y Cuts at: 1972 2557 3730 4097 4482 4499 5951 6037 6049 Size: 585 1173 367 385 17 1452 86 12 Cuts at: 6049 6395 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1173 585 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3221 3221 Size: 6400 Bsu36I CC'TnA_GG Cuts at: 3115 3115 Size: 6400 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4909 Fragments arranged by size: 4909 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5661 Fragments arranged by size: 5661 683 56 BtsI GCAGTG_nn' Cuts at: 3299 3916 4710 4730 3299 Size: 617 794 20 4969 Fragments arranged by size: 4969 794 617 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6400 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6365 DraI TTT'AAA Cuts at: 3704 3704 Size: 6400 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3392 5407 809 Size: 507 2076 2015 1802 Fragments arranged by size: 2076 2015 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2749 3280 3529 Size: 54 1425 50 275 18 919 531 249 Cuts at: 3529 3847 4736 6365 8 Size: 318 889 1629 43 Fragments arranged by size: 1629 1425 919 889 531 318 275 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3847 1537 Size: 275 2035 4090 Fragments arranged by size: 4090 2035 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2979 4330 1353 Size: 1626 1351 3423 Fragments arranged by size: 3423 1626 1351 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4987 5361 5507 387 Size: 1064 138 3398 374 146 1280 Fragments arranged by size: 3398 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2082 2082 Size: 6400 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2403 2982 3135 3192 4463 5060 Size: 393 462 309 579 153 57 1271 597 Cuts at: 5060 5847 6115 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1271 787 597 579 462 393 309 268 153 57 EcoRI G'AATT_C Cuts at: 3709 4087 3709 Size: 378 6022 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2383 2922 2383 Size: 539 5861 FspI TGC'GCA Cuts at: 4906 4906 Size: 6400 HaeII r_GCGC'y Cuts at: 1678 5547 1678 Size: 3869 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2867 2899 3588 3620 5041 Size: 32 135 32 1295 32 689 32 1421 Cuts at: 5041 5073 5115 5147 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1421 1295 689 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3646 3744 3990 669 Size: 988 281 1708 98 246 3079 Fragments arranged by size: 3079 1708 988 281 246 98 HindIII A'AGCT_T Cuts at: 748 3054 748 Size: 2306 4094 HpaI GTT'AAC Cuts at: 3646 3990 3646 Size: 344 6056 KpnI G_GTAC'C Cuts at: 3725 3725 Size: 6400 MfeI C'AATT_G Cuts at: 2292 3300 3999 2292 Size: 1008 699 4693 Fragments arranged by size: 4693 1008 699 MluI A'CGCG_T Cuts at: 6276 6276 Size: 6400 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2293 3547 5514 5698 1305 Size: 988 1254 1967 184 2007 Fragments arranged by size: 2007 1967 1254 988 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2751 3282 10 Size: 54 1425 343 919 531 3128 Fragments arranged by size: 3128 1425 919 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2369 2456 2952 2993 3443 4358 4717 Size: 648 1203 87 496 41 450 915 359 Cuts at: 4717 4876 518 Size: 159 2042 Fragments arranged by size: 2042 1203 915 648 496 450 359 159 87 41 MspA1I CmG'CkG Cuts at: 1606 2381 3816 4493 5641 5886 1606 Size: 775 1435 677 1148 245 2120 Fragments arranged by size: 2120 1435 1148 775 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4909 Fragments arranged by size: 4909 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6400 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6400 NotI GC'GGCC_GC Cuts at: 3847 3847 Size: 6400 NspI r_CATG'y Cuts at: 1132 1229 3642 3758 5303 1132 Size: 97 2413 116 1545 2229 Fragments arranged by size: 2413 2229 1545 116 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6400 PciI A'CATG_T Cuts at: 3638 5299 3638 Size: 1661 4739 PflMI CCAn_nnn'nTGG Cuts at: 1108 3505 1108 Size: 2397 4003 PfoI T'CCnGG_A Cuts at: 2177 2177 Size: 6400 PmeI GTTT'AAAC Cuts at: 3704 3704 Size: 6400 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4469 4501 5982 6014 4469 Size: 32 1481 32 4855 Fragments arranged by size: 4855 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6400 PsiI TTA'TAA Cuts at: 3062 3213 3970 3062 Size: 151 757 5492 Fragments arranged by size: 5492 757 151 PstI C_TGCA'G Cuts at: 830 2890 3752 830 Size: 2060 862 3478 Fragments arranged by size: 3478 2060 862 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6400 PvuII CAG'CTG Cuts at: 2381 2381 Size: 6400 SacI G_AGCT'C Cuts at: 2084 2084 Size: 6400 SalI G'TCGA_C Cuts at: 1936 3742 1936 Size: 1806 4594 SbfI CC_TGCA'GG Cuts at: 3752 3752 Size: 6400 ScaI AGT'ACT Cuts at: 4648 6360 4648 Size: 1712 4688 SfcI C'TryA_G Cuts at: 826 1045 2650 2886 3748 4883 5564 5755 826 Size: 219 1605 236 862 1135 681 191 1471 Fragments arranged by size: 1605 1471 1135 862 681 236 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6400 SmaI CCC'GGG Cuts at: 1872 3727 1872 Size: 1855 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2639 3397 4504 5405 5667 820 Size: 197 926 696 758 1107 901 262 1553 Fragments arranged by size: 1553 1107 926 901 758 696 262 197 SnaBI TAC'GTA Cuts at: 493 4191 493 Size: 3698 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6400 SphI G_CATG'C Cuts at: 1132 3758 1132 Size: 2626 3774 SspI AAT'ATT Cuts at: 5 52 2680 2984 4324 5 Size: 47 2628 304 1340 2081 Fragments arranged by size: 2628 2081 1340 304 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6400 StyI C'CwwG_G Cuts at: 513 1065 2004 2073 2165 3839 513 Size: 552 939 69 92 1674 3074 Fragments arranged by size: 3074 1674 939 552 92 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2590 4427 4444 4597 4782 5227 325 Size: 1255 1010 1837 17 153 185 445 1498 Fragments arranged by size: 1837 1498 1255 1010 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4646 6358 96 Size: 274 80 33 51 1443 2669 1712 138 Fragments arranged by size: 2669 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6360 6392 6360 Size: 32 6368 Tth111I GACn'n_nGTC Cuts at: 1316 3308 1316 Size: 1992 4408 XbaI T'CTAG_A Cuts at: 3736 3736 Size: 6400 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2276 3502 2185 Size: 91 1226 5083 Fragments arranged by size: 5083 1226 91 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6400 XmaI C'CCGG_G Cuts at: 1870 3725 1870 Size: 1855 4545 XmnI GAAnn'nnTTC Cuts at: 4529 4529 Size: 6400 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3394 4208 276 Size: 53 83 186 936 1860 814 2468 Fragments arranged by size: 2468 1860 936 814 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI