Restriction map for pFN21AE0372
 (Linear) (Six-base) MAPSORT of: KIEE0372.seq  Check: 9058  from: 1  to: 1695



KIEE0372.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AatII G_ACGT'C

Cuts at:      0    1391    1695 
   Size:       1391     304
 
AccI GT'mk_AC

Cuts at:      0     648    1181    1215    1695 
   Size:        648     533      34     480

  Fragments arranged by size: 

                 648     533     480      34
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89    1187    1695 
   Size:         89    1098     508

  Fragments arranged by size: 

                1098     508      89
 
AflII C'TTAA_G

Cuts at:      0    1392    1695 
   Size:       1392     303
 
AflIII A'CryG_T

Cuts at:      0     472    1633    1695 
   Size:        472    1161      62

  Fragments arranged by size: 

                1161     472      62
 
AlwNI CAG_nnn'CTG

Cuts at:      0     127    1695 
   Size:        127    1568
 
ApoI r'AATT_y

Cuts at:      0     400     756    1189    1695 
   Size:        400     356     433     506

  Fragments arranged by size: 

                 506     433     400     356
 
AseI AT'TA_AT

Cuts at:      0     850    1695 
   Size:        850     845
 
BanII G_rGCy'C

Cuts at:      0      79     469    1695 
   Size:         79     390    1226

  Fragments arranged by size: 

                1226     390      79
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       3     797    1695 
   Size:          3     794     898

  Fragments arranged by size: 

                 898     794       3
 
BclI T'GATC_A

Cuts at:      0     513     854    1695 
   Size:        513     341     841

  Fragments arranged by size: 

                 841     513     341
 
BglII A'GATC_T

Cuts at:      0     552    1695 
   Size:        552    1143
 
Bme1580I G_kGCm'C

Cuts at:      0     300    1695 
   Size:        300    1395
 
BmgBI CAC'GTC

Cuts at:      0    1594    1695 
   Size:       1594     101
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     173    1157    1695 
   Size:        173     984     538

  Fragments arranged by size: 

                 984     538     173
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     398     977    1130    1695 
   Size:        398     579     153     565

  Fragments arranged by size: 

                 579     565     398     153
 
Bpu10I CC'TnA_GC

Cuts at:      0    1132    1695 
   Size:       1132     563
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     655    1695 
   Size:        655    1040
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     375    1695 
   Size:        375    1320
 
BsaAI yAC'GTr

Cuts at:      0     475    1695 
   Size:        475    1220
 
BsaHI Gr'CG_yC

Cuts at:      0    1388    1695 
   Size:       1388     307
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1106    1136    1220    1250    1695 
   Size:       1106      30      84      30     445

  Fragments arranged by size: 

                1106     445      84      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     505     680    1133    1695 
   Size:        505     175     453     562

  Fragments arranged by size: 

                 562     505     453     175
 
BsiHKAI G_wGCw'C

Cuts at:      0      79    1695 
   Size:         79    1616
 
BsmI GAATG_Cn'

Cuts at:      0    1177    1695 
   Size:       1177     518
 
Bsp1286I G_dGCh'C

Cuts at:      0      79     300     469    1695 
   Size:         79     221     169    1226

  Fragments arranged by size: 

                1226     221     169      79
 
BsrDI GCAATG_nn'

Cuts at:      0      98    1082    1695 
   Size:         98     984     613

  Fragments arranged by size: 

                 984     613      98
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     889    1695 
   Size:        889     806
 
BstYI r'GATC_y

Cuts at:      0     552    1695 
   Size:        552    1143
 
BstZ17I GTA'TAC

Cuts at:      0    1216    1695 
   Size:       1216     479
 
Bsu36I CC'TnA_GG

Cuts at:      0    1110    1695 
   Size:       1110     585
 
BtsI GCAGTG_nn'

Cuts at:      0    1294    1695 
   Size:       1294     401
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1387    1695 
   Size:       1387     308
 
EaeI y'GGCC_r

Cuts at:      0     744    1275    1524    1695 
   Size:        744     531     249     171

  Fragments arranged by size: 

                 744     531     249     171
 
EarI CTCTTCn'nnn_

Cuts at:      0     974    1695 
   Size:        974     721
 
EcoICRI GAG'CTC

Cuts at:      0      77    1695 
   Size:         77    1618
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     398     977    1130    1187    1695 
   Size:         89     309     579     153      57     508

  Fragments arranged by size: 

                 579     508     309     153      89      57
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     378     917    1695 
   Size:        378     539     778

  Fragments arranged by size: 

                 778     539     378
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     862     894    1583    1615    1695 
   Size:        862      32     689      32      80

  Fragments arranged by size: 

                 862     689      80      32      32
 
HincII GTy'rAC

Cuts at:      0    1641    1695 
   Size:       1641      54
 
HindIII A'AGCT_T

Cuts at:      0    1049    1695 
   Size:       1049     646
 
HpaI GTT'AAC

Cuts at:      0    1641    1695 
   Size:       1641      54
 
MfeI C'AATT_G

Cuts at:      0     287    1295    1695 
   Size:        287    1008     400

  Fragments arranged by size: 

                1008     400     287
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     288    1542    1695 
   Size:        288    1254     153

  Fragments arranged by size: 

                1254     288     153
 
MscI TGG'CCA

Cuts at:      0     746    1277    1695 
   Size:        746     531     418

  Fragments arranged by size: 

                 746     531     418
 
MslI CAynn'nnrTG

Cuts at:      0     364     451     947     988    1438    1695 
   Size:        364      87     496      41     450     257

  Fragments arranged by size: 

                 496     450     364     257      87      41
 
MspA1I CmG'CkG

Cuts at:      0     376    1695 
   Size:        376    1319
 
NspI r_CATG'y

Cuts at:      0    1637    1695 
   Size:       1637      58
 
PciI A'CATG_T

Cuts at:      0    1633    1695 
   Size:       1633      62
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1500    1695 
   Size:       1500     195
 
PfoI T'CCnGG_A

Cuts at:      0     172    1695 
   Size:        172    1523
 
PsiI TTA'TAA

Cuts at:      0    1057    1208    1695 
   Size:       1057     151     487

  Fragments arranged by size: 

                1057     487     151
 
PstI C_TGCA'G

Cuts at:      0     885    1695 
   Size:        885     810
 
PvuII CAG'CTG

Cuts at:      0     376    1695 
   Size:        376    1319
 
SacI G_AGCT'C

Cuts at:      0      79    1695 
   Size:         79    1616
 
SfcI C'TryA_G

Cuts at:      0     645     881    1695 
   Size:        645     236     814

  Fragments arranged by size: 

                 814     645     236
 
SmlI C'TyrA_G

Cuts at:      0     634    1392    1695 
   Size:        634     758     303

  Fragments arranged by size: 

                 758     634     303
 
SspI AAT'ATT

Cuts at:      0     675     979    1695 
   Size:        675     304     716

  Fragments arranged by size: 

                 716     675     304
 
StyI C'CwwG_G

Cuts at:      0      68     160    1695 
   Size:         68      92    1535

  Fragments arranged by size: 

                1535      92      68
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     585    1695 
   Size:        585    1110
 
Tth111I GACn'n_nGTC

Cuts at:      0    1303    1695 
   Size:       1303     392
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     180     271    1497    1695 
   Size:        180      91    1226     198

  Fragments arranged by size: 

                1226     198     180      91
 
ZraI GAC'GTC

Cuts at:      0    1389    1695 
   Size:       1389     306
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AflII   AflIII    AlwNI     ApoI     AseI
    BanII     BbsI     BclI    BglII Bme1580I    BmgBI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI  BsiHKAI
     BsmI Bsp1286I    BsrDI   BstAPI    BstYI  BstZ17I   Bsu36I     BtsI
     DrdI     EaeI     EarI  EcoICRI  Eco57MI     FalI    Hin4I   HincII
  HindIII     HpaI     MfeI     MmeI     MscI     MslI   MspA1I     NspI
     PciI    PflMI     PfoI     PsiI     PstI    PvuII     SacI     SfcI
     SmlI     SspI     StyI    TaqII  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
     BlpI     BmrI     BmtI    BsaBI    BsaWI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII     KasI     KpnI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI
     TstI     XbaI     XhoI     XmaI     XmnI
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