(Circular) (Six-base) MAPSORT of: pFN21AE1986.seq Check: 9557 from: 1 to: 6355 pFN21AE1986 6355 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2660 2660 Size: 6355 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4165 278 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3698 1937 Size: 1761 4594 Acc65I G'GTAC_C Cuts at: 3092 3676 3092 Size: 584 5771 AclI AA'CG_TT Cuts at: 1563 4482 4855 1563 Size: 2919 373 3063 Fragments arranged by size: 3063 2919 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4418 5802 6070 1632 Size: 2786 1384 268 1917 Fragments arranged by size: 2786 1917 1384 268 AfeI AGC'GCT Cuts at: 3296 3296 Size: 6355 AflII C'TTAA_G Cuts at: 820 1017 2986 820 Size: 197 1969 4189 Fragments arranged by size: 4189 1969 197 AflIII A'CryG_T Cuts at: 1203 2865 5254 6231 1203 Size: 1662 2389 977 1327 Fragments arranged by size: 2389 1662 1327 977 AgeI A'CCGG_T Cuts at: 5186 5186 Size: 6355 AhdI GACnn_n'nnGTC Cuts at: 5084 5084 Size: 6355 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6355 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6355 AlwNI CAG_nnn'CTG Cuts at: 2096 2587 4098 5670 2096 Size: 491 1511 1572 2781 Fragments arranged by size: 2781 1572 1511 491 ApaLI G'TGCA_C Cuts at: 4412 5568 4412 Size: 1156 5199 ApoI r'AATT_y Cuts at: 1493 2573 2642 3664 3890 4042 6151 1493 Size: 1080 69 1022 226 152 2109 1697 Fragments arranged by size: 2109 1697 1080 1022 226 152 69 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 3680 1870 Size: 73 1737 4545 Fragments arranged by size: 4545 1737 73 BamHI G'GATC_C Cuts at: 3685 4052 3685 Size: 367 5988 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2125 2228 3092 3676 5131 Size: 325 217 627 338 103 864 584 1455 Cuts at: 5131 618 Size: 1842 Fragments arranged by size: 1842 1455 864 627 584 338 325 217 103 BanII G_rGCy'C Cuts at: 1391 2985 3545 3674 1391 Size: 1594 560 129 4072 Fragments arranged by size: 4072 1594 560 129 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2025 3028 928 Size: 971 126 1003 4255 Fragments arranged by size: 4255 1003 971 126 BbvCI CC'TCA_GC Cuts at: 2086 2086 Size: 6355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4546 4580 1903 Size: 34 2609 34 3678 Fragments arranged by size: 3678 2609 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2444 2591 4247 5457 2444 Size: 147 1656 1210 3342 Fragments arranged by size: 3342 1656 1210 147 BclI T'GATC_A Cuts at: 1548 1548 Size: 6355 BfrBI ATG'CAT Cuts at: 2731 2731 Size: 6355 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4966 136 Size: 107 122 71 631 605 3294 1525 Fragments arranged by size: 3294 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6350 6350 Size: 6355 BlpI GC'TnA_GC Cuts at: 3772 3772 Size: 6355 Bme1580I G_kGCm'C Cuts at: 1790 4416 5572 1790 Size: 2626 1156 2573 Fragments arranged by size: 2626 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6355 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2328 3555 4086 Size: 416 53 83 399 24 911 1227 531 Cuts at: 4086 5044 442 Size: 958 1753 Fragments arranged by size: 1753 1227 958 911 531 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2517 3376 5015 1239 Size: 1278 859 1639 2579 Fragments arranged by size: 2579 1639 1278 859 Bpu10I CC'TnA_GC Cuts at: 2086 2086 Size: 6355 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4480 5345 5643 4480 Size: 865 298 5192 Fragments arranged by size: 5192 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2101 3433 5018 882 Size: 617 602 1332 1585 2219 Fragments arranged by size: 2219 1585 1332 617 602 BsaAI yAC'GTr Cuts at: 493 1204 2311 4146 493 Size: 711 1107 1835 2702 Fragments arranged by size: 2702 1835 1107 711 BsaBI GATnn'nnATC Cuts at: 2951 6349 2951 Size: 3398 2957 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4162 4544 5179 Size: 53 83 186 936 476 2153 382 635 Cuts at: 5179 275 Size: 1451 Fragments arranged by size: 2153 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4788 5186 5460 5607 6018 4788 Size: 398 274 147 411 5125 Fragments arranged by size: 5125 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2254 2284 3237 3267 1178 Size: 30 165 30 851 30 953 30 4266 Fragments arranged by size: 4266 953 851 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2008 1186 Size: 822 5533 BseYI C'CCAG_C Cuts at: 5558 6054 5558 Size: 496 5859 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 1163 Size: 573 948 4834 Fragments arranged by size: 4834 948 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 3805 4566 5594 1540 Size: 275 186 39 1765 761 1028 2301 Fragments arranged by size: 2301 1765 1028 761 275 186 39 BsiHKAI G_wGCw'C Cuts at: 3219 4416 4501 5572 3219 Size: 1197 85 1071 4002 Fragments arranged by size: 4002 1197 1071 85 BsmI GAATG_Cn' Cuts at: 3866 3959 3866 Size: 93 6262 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2985 3219 3545 3674 4416 4501 5572 Size: 399 1195 234 326 129 742 85 1071 Cuts at: 5572 1391 Size: 2174 Fragments arranged by size: 2174 1195 1071 742 399 326 234 129 85 BspEI T'CCGG_A Cuts at: 6018 6018 Size: 6355 BspHI T'CATG_A Cuts at: 4244 5974 4244 Size: 1730 4625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2660 3710 844 Size: 1816 1050 3489 Fragments arranged by size: 3489 1816 1050 BsrBI CCG'CTC Cuts at: 1394 1823 4242 1394 Size: 429 2419 3507 Fragments arranged by size: 3507 2419 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4850 5024 65 Size: 1183 3602 174 1396 Fragments arranged by size: 3602 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3025 4999 5186 1700 Size: 110 1215 1974 187 2869 Fragments arranged by size: 2869 1974 1215 187 110 BsrGI T'GTAC_A Cuts at: 96 2868 96 Size: 2772 3583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6355 BssSI C'ACGA_G Cuts at: 4415 5427 4415 Size: 1012 5343 BstAPI GCAn_nnn'nTGC Cuts at: 794 2402 2587 3405 794 Size: 1608 185 818 3744 Fragments arranged by size: 3744 1608 818 185 BstBI TT'CG_AA Cuts at: 5159 5159 Size: 6355 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6355 BstXI CCAn_nnnn'nTGG Cuts at: 3058 3119 3058 Size: 61 6294 BstYI r'GATC_y Cuts at: 1972 3685 4052 4437 4454 5906 5992 6004 6350 Size: 1713 367 385 17 1452 86 12 346 Cuts at: 6350 1972 Size: 1977 Fragments arranged by size: 1977 1713 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2182 2182 Size: 6355 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 2112 3057 3096 513 Size: 552 939 57 51 945 39 3772 Fragments arranged by size: 3772 945 939 552 57 51 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3248 487 Size: 683 56 2022 3594 Fragments arranged by size: 3594 2022 683 56 BtsI GCAGTG_nn' Cuts at: 2473 2998 3871 4665 4685 2473 Size: 525 873 794 20 4143 Fragments arranged by size: 4143 873 794 525 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6320 DraI TTT'AAA Cuts at: 2812 3659 2812 Size: 847 5508 DraIII CAC_nnn'GTG Cuts at: 2257 2257 Size: 6355 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5362 809 Size: 507 4046 1802 Fragments arranged by size: 4046 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3802 4691 6320 Size: 54 1425 50 275 18 1972 889 1629 Cuts at: 6320 8 Size: 43 Fragments arranged by size: 1972 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3802 1537 Size: 275 1990 4090 Fragments arranged by size: 4090 1990 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2805 4285 1353 Size: 1452 1480 3423 Fragments arranged by size: 3423 1480 1452 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2585 4942 5316 5462 387 Size: 1064 138 996 2357 374 146 1280 Fragments arranged by size: 2357 1280 1064 996 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2517 3376 4418 5015 5802 6070 1239 Size: 393 885 859 1042 597 787 268 1524 Fragments arranged by size: 1524 1042 885 859 787 597 393 268 EcoRI G'AATT_C Cuts at: 3664 4042 3664 Size: 378 5977 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3346 3346 Size: 6355 FspI TGC'GCA Cuts at: 4861 4861 Size: 6355 HaeII r_GCGC'y Cuts at: 1678 3298 5502 1678 Size: 1620 2204 2531 Fragments arranged by size: 2531 2204 1620 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2344 2376 2689 2721 3237 Size: 32 135 32 772 32 313 32 516 Cuts at: 3237 3239 3269 3271 4996 5028 5070 5102 1373 Size: 2 30 2 1725 32 42 32 2626 Fragments arranged by size: 2626 1725 772 516 313 135 42 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3699 3945 669 Size: 988 281 1761 246 3079 Fragments arranged by size: 3079 1761 988 281 246 HindIII A'AGCT_T Cuts at: 748 3623 748 Size: 2875 3480 HpaI GTT'AAC Cuts at: 3945 3945 Size: 6355 KpnI G_GTAC'C Cuts at: 3096 3680 3096 Size: 584 5771 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2581 3192 3559 5469 5653 1305 Size: 1276 611 367 1910 184 2007 Fragments arranged by size: 2007 1910 1276 611 367 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4533 Fragments arranged by size: 4533 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2169 2370 3056 3117 4313 4672 4831 Size: 648 1003 201 686 61 1196 359 159 Cuts at: 4831 518 Size: 2042 Fragments arranged by size: 2042 1196 1003 686 648 359 201 159 61 MspA1I CmG'CkG Cuts at: 1606 2063 2476 3771 4448 5596 5841 1606 Size: 457 413 1295 677 1148 245 2120 Fragments arranged by size: 2120 1295 1148 677 457 413 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2112 3057 3096 513 Size: 552 939 108 945 39 3772 Fragments arranged by size: 3772 945 939 552 108 39 NdeI CA'TA_TG Cuts at: 387 3114 387 Size: 2727 3628 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NsiI A_TGCA'T Cuts at: 2733 2733 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 2146 2869 3713 5258 1132 Size: 97 917 723 844 1545 2229 Fragments arranged by size: 2229 1545 917 844 723 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6355 PciI A'CATG_T Cuts at: 2865 5254 2865 Size: 2389 3966 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 1108 Size: 1149 5206 PfoI T'CCnGG_A Cuts at: 2418 2593 2418 Size: 175 6180 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3629 3661 4424 4456 5937 5969 3629 Size: 32 763 32 1481 32 4015 Fragments arranged by size: 4015 1481 763 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6355 PsiI TTA'TAA Cuts at: 3925 3925 Size: 6355 PstI C_TGCA'G Cuts at: 830 2468 2583 2895 2916 3707 830 Size: 1638 115 312 21 791 3478 Fragments arranged by size: 3478 1638 791 312 115 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 SacII CC_GC'GG Cuts at: 2064 2064 Size: 6355 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SbfI CC_TGCA'GG Cuts at: 3707 3707 Size: 6355 ScaI AGT'ACT Cuts at: 3005 4603 6315 3005 Size: 1598 1712 3045 Fragments arranged by size: 3045 1712 1598 SfcI C'TryA_G Cuts at: 826 1045 2464 2488 2579 2891 2912 3185 3703 Size: 219 1419 24 91 312 21 273 518 Cuts at: 3703 4838 5519 5710 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1419 1135 681 518 312 273 219 191 91 24 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 3682 1872 Size: 1810 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2986 4459 5360 5622 820 Size: 197 926 1043 1473 901 262 1553 Fragments arranged by size: 1553 1473 1043 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4146 493 Size: 3653 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 2146 3713 1132 Size: 1014 1567 3774 Fragments arranged by size: 3774 1567 1014 SspI AAT'ATT Cuts at: 5 52 4279 5 Size: 47 4227 2081 Fragments arranged by size: 4227 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6355 StyI C'CwwG_G Cuts at: 513 1065 2004 2112 3057 3096 3487 3615 3794 Size: 552 939 108 945 39 391 128 179 Cuts at: 3794 513 Size: 3074 Fragments arranged by size: 3074 945 939 552 391 179 128 108 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 3107 3348 3415 3665 4382 4399 Size: 1255 446 1081 241 67 250 717 17 Cuts at: 4399 4552 4737 5182 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1081 717 446 445 250 241 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 2868 3003 Size: 274 80 33 51 1443 678 213 135 Cuts at: 3003 3608 4601 6313 96 Size: 605 993 1712 138 Fragments arranged by size: 1712 1443 993 678 605 274 213 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6315 6347 6315 Size: 32 6323 Tth111I GACn'n_nGTC Cuts at: 1316 3251 1316 Size: 1935 4420 XbaI T'CTAG_A Cuts at: 3691 3691 Size: 6355 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6355 XmaI C'CCGG_G Cuts at: 1870 3680 1870 Size: 1810 4545 XmnI GAAnn'nnTTC Cuts at: 3153 4484 3153 Size: 1331 5024 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4163 276 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI