Restriction map for
pFN21AE1986
(Linear) (Six-base) MAPSORT of: KIEE1986.seq Check: 5272 from: 1 to: 1650
KIEE1986.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 655 1650
Size: 655 995
Acc65I G'GTAC_C
Cuts at: 0 1087 1650
Size: 1087 563
AfeI AGC'GCT
Cuts at: 0 1291 1650
Size: 1291 359
AflII C'TTAA_G
Cuts at: 0 981 1650
Size: 981 669
AflIII A'CryG_T
Cuts at: 0 860 1650
Size: 860 790
AlwNI CAG_nnn'CTG
Cuts at: 0 91 582 1650
Size: 91 491 1068
Fragments arranged by size:
1068 491 91
ApoI r'AATT_y
Cuts at: 0 568 637 1650
Size: 568 69 1013
Fragments arranged by size:
1013 568 69
BanI G'GyrC_C
Cuts at: 0 120 223 1087 1650
Size: 120 103 864 563
Fragments arranged by size:
864 563 120 103
BanII G_rGCy'C
Cuts at: 0 980 1540 1650
Size: 980 560 110
Fragments arranged by size:
980 560 110
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 1023 1650
Size: 20 1003 627
Fragments arranged by size:
1003 627 20
BbvCI CC'TCA_GC
Cuts at: 0 81 1650
Size: 81 1569
BciVI GTATCCnnnnn_n'
Cuts at: 0 439 586 1650
Size: 439 147 1064
Fragments arranged by size:
1064 439 147
BfrBI ATG'CAT
Cuts at: 0 726 1650
Size: 726 924
BmrI ACTGGGnnnn_n'
Cuts at: 0 323 1550 1650
Size: 323 1227 100
Fragments arranged by size:
1227 323 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1371 1650
Size: 512 859 279
Fragments arranged by size:
859 512 279
Bpu10I CC'TnA_GC
Cuts at: 0 81 1650
Size: 81 1569
BsaI GGTCTCn'nnnn_
Cuts at: 0 96 1428 1650
Size: 96 1332 222
Fragments arranged by size:
1332 222 96
BsaAI yAC'GTr
Cuts at: 0 306 1650
Size: 306 1344
BsaBI GATnn'nnATC
Cuts at: 0 946 1650
Size: 946 704
BsaHI Gr'CG_yC
Cuts at: 0 4 1650
Size: 4 1646
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 249 279 1232 1262 1650
Size: 249 30 953 30 388
Fragments arranged by size:
953 388 249 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3 1650
Size: 3 1647
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1650
Size: 679 971
BsiEI CG_ry'CG
Cuts at: 0 35 1650
Size: 35 1615
BsiHKAI G_wGCw'C
Cuts at: 0 1214 1650
Size: 1214 436
Bsp1286I G_dGCh'C
Cuts at: 0 980 1214 1540 1650
Size: 980 234 326 110
Fragments arranged by size:
980 326 234 110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 655 1650
Size: 655 995
BsrFI r'CCGG_y
Cuts at: 0 1020 1650
Size: 1020 630
BsrGI T'GTAC_A
Cuts at: 0 863 1650
Size: 863 787
BstAPI GCAn_nnn'nTGC
Cuts at: 0 397 582 1400 1650
Size: 397 185 818 250
Fragments arranged by size:
818 397 250 185
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1053 1114 1650
Size: 1053 61 536
Fragments arranged by size:
1053 536 61
Bsu36I CC'TnA_GG
Cuts at: 0 177 1650
Size: 177 1473
BtgI C'CryG_G
Cuts at: 0 56 107 1052 1091 1650
Size: 56 51 945 39 559
Fragments arranged by size:
945 559 56 51 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1243 1650
Size: 1243 407
BtsI GCAGTG_nn'
Cuts at: 0 468 993 1650
Size: 468 525 657
Fragments arranged by size:
657 525 468
DraI TTT'AAA
Cuts at: 0 807 1650
Size: 807 843
DraIII CAC_nnn'GTG
Cuts at: 0 252 1650
Size: 252 1398
EarI CTCTTCn'nnn_
Cuts at: 0 800 1650
Size: 800 850
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 580 1650
Size: 580 1070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1371 1650
Size: 512 859 279
Fragments arranged by size:
859 512 279
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1341 1650
Size: 1341 309
HaeII r_GCGC'y
Cuts at: 0 1293 1650
Size: 1293 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 684 716 1232 1234 1264 1266
Size: 339 32 313 32 516 2 30 2
Cuts at: 1266 1650
Size: 384
Fragments arranged by size:
516 384 339 313 32 32 30 2
2
HindIII A'AGCT_T
Cuts at: 0 1618 1650
Size: 1618 32
KpnI G_GTAC'C
Cuts at: 0 1091 1650
Size: 1091 559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1187 1554 1650
Size: 576 611 367 96
Fragments arranged by size:
611 576 367 96
MslI CAynn'nnrTG
Cuts at: 0 164 365 1051 1112 1650
Size: 164 201 686 61 538
Fragments arranged by size:
686 538 201 164 61
MspA1I CmG'CkG
Cuts at: 0 58 471 1650
Size: 58 413 1179
Fragments arranged by size:
1179 413 58
NcoI C'CATG_G
Cuts at: 0 107 1052 1091 1650
Size: 107 945 39 559
Fragments arranged by size:
945 559 107 39
NdeI CA'TA_TG
Cuts at: 0 1109 1650
Size: 1109 541
NsiI A_TGCA'T
Cuts at: 0 728 1650
Size: 728 922
NspI r_CATG'y
Cuts at: 0 141 864 1650
Size: 141 723 786
Fragments arranged by size:
786 723 141
PciI A'CATG_T
Cuts at: 0 860 1650
Size: 860 790
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 1650
Size: 252 1398
PfoI T'CCnGG_A
Cuts at: 0 413 588 1650
Size: 413 175 1062
Fragments arranged by size:
1062 413 175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1624 1650
Size: 1624 26
PstI C_TGCA'G
Cuts at: 0 463 578 890 911 1650
Size: 463 115 312 21 739
Fragments arranged by size:
739 463 312 115 21
SacII CC_GC'GG
Cuts at: 0 59 1650
Size: 59 1591
ScaI AGT'ACT
Cuts at: 0 1000 1650
Size: 1000 650
SfcI C'TryA_G
Cuts at: 0 459 483 574 886 907 1180 1650
Size: 459 24 91 312 21 273 470
Fragments arranged by size:
470 459 312 273 91 24 21
SmlI C'TyrA_G
Cuts at: 0 981 1650
Size: 981 669
SphI G_CATG'C
Cuts at: 0 141 1650
Size: 141 1509
StyI C'CwwG_G
Cuts at: 0 107 1052 1091 1482 1610 1650
Size: 107 945 39 391 128 40
Fragments arranged by size:
945 391 128 107 40 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 1102 1343 1410 1650
Size: 21 1081 241 67 240
Fragments arranged by size:
1081 241 240 67 21
TatI w'GTAC_w
Cuts at: 0 650 863 998 1603 1650
Size: 650 213 135 605 47
Fragments arranged by size:
650 605 213 135 47
Tth111I GACn'n_nGTC
Cuts at: 0 1246 1650
Size: 1246 404
XmnI GAAnn'nnTTC
Cuts at: 0 1148 1650
Size: 1148 502
Enzymes that do cut:
AarI Acc65I AfeI AflII AflIII AlwNI ApoI BanI
BanII BbsI BbvCI BciVI BfrBI BmrI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BsrGI BstAPI BstXI Bsu36I
BtgI BtgZI BtsI DraI DraIII EarI EciI Eco57MI
FalI HaeII Hin4I HindIII KpnI MmeI MslI MspA1I
NcoI NdeI NsiI NspI PciI PflMI PfoI PpiI
PstI SacII ScaI SfcI SmlI SphI StyI TaqII
TatI Tth111I XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BclI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI BpuEI BsaWI BseYI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI
BstBI BstEII BstYI BstZ17I ClaI CspCI DrdI EaeI
EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HincII HpaI KasI MfeI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TstI XbaI XcmI XhoI XmaI
ZraI