################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## Acc65I AccIII AclI AcsI AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbeI BbrPI BbvCI BfaI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BspDI BspEI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI NarI NdeI NheI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 435 GACGTC GACGT^C AccI 715 GTCTAC GT^[AC][GT]AC AccII 1174 CGCG CG^CG AcyI 435 GACGTC G[GA]^CG[CT]C AflII 415 CTTAAG C^TTAAG AocI 802 CCTGAGG CC^T[ACGT]AGG AspHI 1159 GTGCTC G[AT]GC[AT]^C AspI 1048 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 581 TGCGCA TGC^GCA BbsI 60 GAAGAC GAAGAC(2/6) BcgI 704 GCAACCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 385 GGATAC GTATCC(6/5) BclI 671 TGATCA T^GATCA BfrI 415 CTTAAG C^TTAAG BglI 869 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 60 GAAGAC GAAGAC(2/6) BsaHI 435 GACGTC G[GA]^CG[CT]C BsgI 1246 CTGCAC GTGCAG(16/14) BsiHKAI 1159 GTGCTC G[AT]GC[AT]^C BsmI 780 GCATTC GAATGC(1/-1) BspHI 1058 TCATGA T^CATGA BspLU11I 563 ACATGT A^CATGT BsrBI 819 CCGCTC CCGCTC(-3/-3) BsrDI 1278 CATTGC GCAATG(2/0) BssSI 226 CACGAG CACGAG(-5/-1) BstUI 1174 CGCG CG^CG Bsu36I 802 CCTGAGG CC^T[ACGT]AGG DrdI 558 GACCTACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 802 CCTGAGG CC^T[ACGT]AGG EcoNI 660 CCTTACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 464 CCATGG C^C[AT][AT]GG FbaI 671 TGATCA T^GATCA FspI 581 TGCGCA TGC^GCA HaeII 1193 AGCGCC [GA]GCGC^[CT] HgaI 1052 GCGTC GACGC(5/10) Hin1I 435 GACGTC G[GA]^CG[CT]C HincII 541 GTCAAC GT[CT]^[GA]AC HindII 541 GTCAAC GT[CT]^[GA]AC HinfI 63 GACTC G^A[ACGT]TC MseI 416 TTAA T^TAA MslI 1209 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1174 CGCG CG^CG NcoI 464 CCATGG C^CATGG NotI 875 GCGGCCGC GC^GGCCGC PaeR7I 1131 CTCGAG C^TCGAG PflFI 1048 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 63 GACTC GAGTC(4/5) PstI 442 CTGCAG CTGCA^G PvuI 1095 CGATCG CGAT^CG RcaI 1058 TCATGA T^CATGA SapI 796 GAAGAGC GCTCTTC(1/4) SexAI 956 ACCAGGT A^CC[AT]GGT SgrAI 1195 CGCCGGCG C[GA]^CCGG[CT]G StuI 1127 AGGCCT AGG^CCT StyI 464 CCATGG C^C[AT][AT]GG Tru9I 416 TTAA T^TAA Tsp509I 347 AATT ^AATT Tth111I 1048 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 464 CCATGGATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1131 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 203 ACGTGT A^C[GA][CT]GT 563 ACATGT AlwNI 546 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 677 CAGCATCTG BanII 486 GAGCCC G[GA]GC[CT]^C 732 GGGCTC BsaI 556 GAGACC GGTCTC(1/5) 949 GAGACC BseRI 843 CTCCTC GAGGAG(10/8) 265 GAGGAG BsmAI 556 GAGAC GTCTC(1/5) 949 GAGAC Bsp1407I 206 TGTACA T^GTACA 1304 TGTACA BspMI 578 ACCTGC ACCTGC(4/8) 444 GCAGGT BsrFI 642 GCCGGC [GA]^CCGG[CT] 1196 GCCGGC BsrGI 206 TGTACA T^GTACA 1304 TGTACA BsrI 451 CCAGT ACTGG(1/-1) 812 CCAGT Cfr10I 642 GCCGGC [GA]^CCGG[CT] 1196 GCCGGC EagI 135 CGGCCG C^GGCCG 876 CGGCCG EclXI 135 CGGCCG C^GGCCG 876 CGGCCG Eco52I 135 CGGCCG C^GGCCG 876 CGGCCG NaeI 642 GCCGGC GCC^GGC 1196 GCCGGC NgoMIV 642 GCCGGC G^CCGGC 1196 GCCGGC PvuII 68 CAGCTG CAG^CTG 761 CAGCTG SfaNI 584 GCATC GCATC(5/9) 679 GCATC SfcI 442 CTGCAG C^T[GA][CT]AG 717 CTACAG SmaI 893 CCCGGG CCC^GGG 1151 CCCGGG SspBI 206 TGTACA T^GTACA 1304 TGTACA XmaCI 893 CCCGGG C^CCGGG 1151 CCCGGG XmaI 893 CCCGGG C^CCGGG 1151 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI AvaII BalI BanI BbvI BcnI BmyI BpmI BsaJI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI RsaI Sau3AI Sau96I ScrFI SmlI TaqI TseI Tsp45I TspRI TthHB8I XhoII