(Circular) (Six-base) MAPSORT of: pFN21AB8130.seq Check: 4097 from: 1 to: 6046 pFN21AB8130 6046 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2440 2440 Size: 6046 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2444 3856 278 Size: 53 83 186 936 908 1412 2468 Fragments arranged by size: 2468 1412 936 908 186 83 53 AccI GT'mk_AC Cuts at: 1937 2721 3389 1937 Size: 784 668 4594 Fragments arranged by size: 4594 784 668 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 2483 4109 5493 5761 1632 Size: 788 63 1626 1384 268 1917 Fragments arranged by size: 1917 1626 1384 788 268 63 AflII C'TTAA_G Cuts at: 820 1017 2420 820 Size: 197 1403 4446 Fragments arranged by size: 4446 1403 197 AflIII A'CryG_T Cuts at: 1203 2208 2568 4945 5922 1203 Size: 1005 360 2377 977 1327 Fragments arranged by size: 2377 1327 1005 977 360 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6046 AlwNI CAG_nnn'CTG Cuts at: 2556 2687 3789 5361 2556 Size: 131 1102 1572 3241 Fragments arranged by size: 3241 1572 1102 131 ApaLI G'TGCA_C Cuts at: 4103 5259 4103 Size: 1156 4890 ApoI r'AATT_y Cuts at: 1493 3355 3581 3733 5842 1493 Size: 1862 226 152 2109 1697 Fragments arranged by size: 2109 1862 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 2898 3136 3156 3371 1870 Size: 73 955 238 20 215 4545 Fragments arranged by size: 4545 955 238 215 73 20 BamHI G'GATC_C Cuts at: 3376 3743 3376 Size: 367 5679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2452 2601 3264 3367 4822 Size: 325 217 627 665 149 663 103 1455 Cuts at: 4822 618 Size: 1842 Fragments arranged by size: 1842 1455 665 663 627 325 217 149 103 BanII G_rGCy'C Cuts at: 1391 2495 2741 3365 1391 Size: 1104 246 624 4072 Fragments arranged by size: 4072 1104 624 246 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2072 928 Size: 971 173 4902 Fragments arranged by size: 4902 971 173 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2698 2732 4237 4271 1903 Size: 34 761 34 1505 34 3678 Fragments arranged by size: 3678 1505 761 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2384 3938 5148 2384 Size: 1554 1210 3282 Fragments arranged by size: 3282 1554 1210 BclI T'GATC_A Cuts at: 1548 2676 1548 Size: 1128 4918 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2880 4657 136 Size: 107 122 71 631 605 1208 1777 1525 Fragments arranged by size: 1777 1525 1208 631 605 122 107 71 BglII A'GATC_T Cuts at: 6041 6041 Size: 6046 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 4107 5263 1790 Size: 2317 1156 2573 Fragments arranged by size: 2573 2317 1156 BmgBI CAC'GTC Cuts at: 1318 2209 2863 3121 1318 Size: 891 654 258 4243 Fragments arranged by size: 4243 891 654 258 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2811 3777 4735 Size: 416 53 83 399 24 1394 966 958 Cuts at: 4735 442 Size: 1753 Fragments arranged by size: 1753 1394 966 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 2282 3149 4706 1239 Size: 816 227 867 1557 2579 Fragments arranged by size: 2579 1557 867 816 227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2237 3152 4171 5036 5334 2237 Size: 915 1019 865 298 2949 Fragments arranged by size: 2949 1019 915 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2555 2948 4709 882 Size: 617 1056 393 1761 2219 Fragments arranged by size: 2219 1761 1056 617 393 BsaAI yAC'GTr Cuts at: 493 1204 3837 493 Size: 711 2633 2702 Fragments arranged by size: 2702 2633 711 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2441 3853 4235 4870 Size: 53 83 186 936 908 1412 382 635 Cuts at: 4870 275 Size: 1451 Fragments arranged by size: 1451 1412 936 908 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4479 4877 5151 5298 5709 4479 Size: 398 274 147 411 4816 Fragments arranged by size: 4816 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2519 2525 2549 2555 1178 Size: 30 165 30 1116 6 24 6 4669 Fragments arranged by size: 4669 1116 165 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2839 1186 Size: 1099 554 4393 Fragments arranged by size: 4393 1099 554 BseYI C'CCAG_C Cuts at: 5249 5745 5249 Size: 496 5550 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3236 1163 Size: 573 1500 3973 Fragments arranged by size: 3973 1500 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2143 2884 3103 3496 4257 5285 Size: 275 186 142 741 219 393 761 1028 Cuts at: 5285 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 741 393 275 219 186 142 BsiHKAI G_wGCw'C Cuts at: 3168 4107 4192 5263 3168 Size: 939 85 1071 3951 Fragments arranged by size: 3951 1071 939 85 BsmI GAATG_Cn' Cuts at: 2785 3557 3650 2785 Size: 772 93 5181 Fragments arranged by size: 5181 772 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2495 2741 3168 3365 4107 4192 5263 Size: 399 705 246 427 197 742 85 1071 Cuts at: 5263 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 705 427 399 246 197 85 BspEI T'CCGG_A Cuts at: 5709 5709 Size: 6046 BspHI T'CATG_A Cuts at: 3063 3935 5665 3063 Size: 872 1730 3444 Fragments arranged by size: 3444 1730 872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2440 2592 3401 844 Size: 1596 152 809 3489 Fragments arranged by size: 3489 1596 809 152 BsrBI CCG'CTC Cuts at: 1394 1823 2826 3933 1394 Size: 429 1003 1107 3507 Fragments arranged by size: 3507 1107 1003 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3282 4541 4715 65 Size: 1183 2034 1259 174 1396 Fragments arranged by size: 2034 1396 1259 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2647 3201 4690 4877 1700 Size: 110 837 554 1489 187 2869 Fragments arranged by size: 2869 1489 837 554 187 110 BsrGI T'GTAC_A Cuts at: 96 2211 3309 96 Size: 2115 1098 2833 Fragments arranged by size: 2833 2115 1098 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 2231 4106 5118 2231 Size: 1875 1012 3159 Fragments arranged by size: 3159 1875 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6046 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6046 BstYI r'GATC_y Cuts at: 1972 2473 2502 3246 3376 3743 4128 4145 5597 Size: 501 29 744 130 367 385 17 1452 Cuts at: 5597 5683 5695 6041 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 744 501 385 367 346 130 86 29 17 12 Bsu36I CC'TnA_GG Cuts at: 2808 2808 Size: 6046 BtgI C'CryG_G Cuts at: 513 1065 2004 2433 2469 3173 513 Size: 552 939 429 36 704 3386 Fragments arranged by size: 3386 939 704 552 429 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3274 487 Size: 683 56 2048 3259 Fragments arranged by size: 3259 2048 683 56 BtsI GCAGTG_nn' Cuts at: 2444 2704 3562 4356 4376 2444 Size: 260 858 794 20 4114 Fragments arranged by size: 4114 858 794 260 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2569 5053 809 Size: 507 1253 2484 1802 Fragments arranged by size: 2484 1802 1253 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2140 2515 Size: 54 1425 50 275 18 291 19 375 Cuts at: 2515 2607 2649 2881 3493 4382 6011 8 Size: 92 42 232 612 889 1629 43 Fragments arranged by size: 1629 1425 889 612 375 291 275 232 92 54 50 43 42 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2140 2881 3493 1537 Size: 275 328 741 612 4090 Fragments arranged by size: 4090 741 612 328 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2403 2720 2796 3976 1353 Size: 1050 317 76 1180 3423 Fragments arranged by size: 3423 1180 1050 317 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2591 4633 5007 5153 387 Size: 1064 138 1002 2042 374 146 1280 Fragments arranged by size: 2042 1280 1064 1002 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2282 2420 2483 3149 4109 4706 Size: 393 423 227 138 63 666 960 597 Cuts at: 4706 5493 5761 1239 Size: 787 268 1524 Fragments arranged by size: 1524 960 787 666 597 423 393 268 227 138 63 EcoNI CCTnn'n_nnAGG Cuts at: 2669 2669 Size: 6046 EcoO109I rG'GnC_Cy Cuts at: 2425 2662 2812 2926 3072 2425 Size: 237 150 114 146 5399 Fragments arranged by size: 5399 237 150 146 114 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 FspI TGC'GCA Cuts at: 2588 4552 2588 Size: 1964 4082 HaeII r_GCGC'y Cuts at: 1678 3202 5193 1678 Size: 1524 1991 2531 Fragments arranged by size: 2531 1991 1524 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 4687 4719 4761 Size: 32 135 32 497 32 2586 32 42 Cuts at: 4761 4793 1373 Size: 32 2626 Fragments arranged by size: 2626 2586 497 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2548 3390 3636 669 Size: 988 281 610 842 246 3079 Fragments arranged by size: 3079 988 842 610 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2883 5160 5344 1305 Size: 1578 2277 184 2007 Fragments arranged by size: 2277 2007 1578 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2123 2517 2609 10 Size: 54 1425 343 291 394 92 3447 Fragments arranged by size: 3447 1425 394 343 291 92 54 MslI CAynn'nnrTG Cuts at: 518 1166 3218 4004 4363 4522 518 Size: 648 2052 786 359 159 2042 Fragments arranged by size: 2052 2042 786 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2075 2161 2768 2880 3462 4139 5287 5532 Size: 469 86 607 112 582 677 1148 245 Cuts at: 5532 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 607 582 469 245 112 86 NaeI GCC'GGC Cuts at: 2649 3203 2649 Size: 554 5492 NcoI C'CATG_G Cuts at: 513 1065 2004 2469 513 Size: 552 939 465 4090 Fragments arranged by size: 4090 939 552 465 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NgoMIV G'CCGG_C Cuts at: 2647 3201 2647 Size: 554 5492 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 2881 3493 2881 Size: 612 5434 NspI r_CATG'y Cuts at: 1132 1229 2572 2761 2893 3097 3343 3404 4949 Size: 97 1343 189 132 204 246 61 1545 Cuts at: 4949 1132 Size: 2229 Fragments arranged by size: 2229 1545 1343 246 204 189 132 97 61 PasI CC'CwG_GG Cuts at: 1348 2055 1348 Size: 707 5339 PciI A'CATG_T Cuts at: 2568 4945 2568 Size: 2377 3669 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6046 PfoI T'CCnGG_A Cuts at: 2040 3318 2040 Size: 1278 4768 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2425 2662 2812 2926 2425 Size: 237 150 114 5545 Fragments arranged by size: 5545 237 150 114 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PspXI vC'TCGA_Gb Cuts at: 3136 3136 Size: 6046 PstI C_TGCA'G Cuts at: 830 2451 3398 830 Size: 1621 947 3478 Fragments arranged by size: 3478 1621 947 PvuI CG_AT'CG Cuts at: 2001 3103 2001 Size: 1102 4944 PvuII CAG'CTG Cuts at: 2075 2768 2075 Size: 693 5353 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SapI GCTCTTCn'nnn_ Cuts at: 2796 2796 Size: 6046 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SexAI A'CCwGG_T Cuts at: 2961 2961 Size: 6046 SfcI C'TryA_G Cuts at: 826 1045 2447 2722 3394 4529 5210 5401 826 Size: 219 1402 275 672 1135 681 191 1471 Fragments arranged by size: 1471 1402 1135 681 672 275 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 3201 1700 Size: 1501 4545 SmaI CCC'GGG Cuts at: 1872 2900 3158 3373 1872 Size: 1028 258 215 4545 Fragments arranged by size: 4545 1028 258 215 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 2420 3136 3167 4150 5051 Size: 197 926 309 168 716 31 983 901 Cuts at: 5051 5313 820 Size: 262 1553 Fragments arranged by size: 1553 983 926 901 716 309 262 197 168 31 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 3134 1517 Size: 1617 4429 StyI C'CwwG_G Cuts at: 513 1065 2004 2469 3485 513 Size: 552 939 465 1016 3074 Fragments arranged by size: 3074 1016 939 552 465 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4073 4090 4243 4428 4873 325 Size: 1255 2493 17 153 185 445 1498 Fragments arranged by size: 2493 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 3309 4292 Size: 274 80 33 51 1443 234 1098 983 Cuts at: 4292 6004 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1098 983 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2519 2551 6006 6038 2519 Size: 32 3455 32 2527 Fragments arranged by size: 3455 2527 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3056 1316 Size: 1740 4306 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 6046 XhoI C'TCGA_G Cuts at: 1943 3136 1943 Size: 1193 4853 XmaI C'CCGG_G Cuts at: 1870 2898 3156 3371 1870 Size: 1028 258 215 4545 Fragments arranged by size: 4545 1028 258 215 XmnI GAAnn'nnTTC Cuts at: 4175 4175 Size: 6046 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2442 3854 276 Size: 53 83 186 936 908 1412 2468 Fragments arranged by size: 2468 1412 936 908 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI