################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI Alw44I ApaI ApaLI AscI Asp700I Asp718I AvaI AviII AvrII BamHI BbeI BbrPI BcgI BclI BcnI BglI BlnI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BspHI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CpoI DraIII DrdI DsaI EagI EclXI Eco52I EcoO65I FbaI FseI FspI HgaI Hin1I KasI KpnI KspI MfeI MluI MroI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 633 GTCGAC GT^[AC][GT]AC AflII 2029 CTTAAG C^TTAAG AhdI 1410 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1598 GATCC GGATC(4/5) AocI 1016 CCTCAGG CC^T[ACGT]AGG AspEI 1410 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2439 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 2320 GGTGCC G^G[CT][GA]CC BbvCI 2300 CCTCAGC CCTCAGC(-5/-2) BfrI 2029 CTTAAG C^TTAAG BglII 676 AGATCT A^GATCT BlpI 817 GCTGAGC GC^T[ACGT]AGC Bpu1102I 817 GCTGAGC GC^T[ACGT]AGC BsaBI 677 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 372 GAGACC GGTCTC(1/5) BsgI 1142 GTGCAG GTGCAG(16/14) BsmAI 372 GAGAC GTCTC(1/5) BspDI 388 ATCGAT AT^CGAT BsrFI 1370 GCCGGC [GA]^CCGG[CT] BstAPI 1625 GCACACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1016 CCTCAGG CC^T[ACGT]AGG CelII 817 GCTGAGC GC^T[ACGT]AGC Cfr10I 1370 GCCGGC [GA]^CCGG[CT] ClaI 388 ATCGAT AT^CGAT DraI 1077 TTTAAA TTT^AAA Eam1105I 1410 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1336 GAAGAG CTCTTC(1/4) Eco81I 1016 CCTCAGG CC^T[ACGT]AGG EcoNI 223 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 385 GATATC GAT^ATC HapII 1371 CCGG C^CGG HpaI 2082 GTTAAC GTT^AAC HpaII 1371 CCGG C^CGG Ksp632I 1336 GAAGAG CTCTTC(1/4) MaeII 631 ACGT A^CGT MamI 677 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 1371 CCGG C^CGG NaeI 1370 GCCGGC GCC^GGC NgoMIV 1370 GCCGGC G^CCGGC PflFI 2439 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 744 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1357 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2122 CTGCAG CTGCA^G SalI 633 GTCGAC G^TCGAC ScaI 607 AGTACT AGT^ACT StuI 2506 AGGCCT AGG^CCT Tth111I 2439 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 744 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 180 CCAGCCTTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AlwNI 152 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1220 CAGAATCTG Aor51HI 662 AGCGCT AGC^GCT 2108 AGCGCT AseI 787 ATTAAT AT^TAAT 882 ATTAAT AsnI 787 ATTAAT AT^TAAT 882 ATTAAT AspHI 1651 GAGCAC G[AT]GC[AT]^C 2183 GTGCTC BalI 444 TGGCCA TGG^CCA 1829 TGGCCA BanII 1328 GGGCTC G[GA]GC[CT]^C 1527 GAGCCC BbsI 331 GAAGAC GAAGAC(2/6) 346 GAAGAC BciVI 1804 GGATAC GTATCC(6/5) 2207 GGATAC BfaI 653 CTAG C^TAG 1171 CTAG BpuAI 331 GAAGAC GAAGAC(2/6) 346 GAAGAC BsiHKAI 1651 GAGCAC G[AT]GC[AT]^C 2183 GTGCTC Bsp1407I 1787 TGTACA T^GTACA 2064 TGTACA BsrGI 1787 TGTACA T^GTACA 2064 TGTACA BssKI 564 CCAGG ^CC[ACGT]GG 1214 CCTGG BstNI 564 CCAGG CC^[AT]GG 1214 CCTGG BstYI 676 AGATCT [GA]^GATC[CT] 1597 AGATCC DpnII 677 GATC ^GATC 1598 GATC DraII 1408 GGGACCC [GA]G^G[ACGT]CC[CT] 1998 AGGTCCT EaeI 444 TGGCCA [CT]^GGCC[GA] 1829 TGGCCA Eco47III 662 AGCGCT AGC^GCT 2108 AGCGCT EcoO109I 1408 GGGACCC [GA]G^G[ACGT]CC[CT] 1998 AGGTCCT EcoRI 1705 GAATTC G^AATTC 2127 GAATTC EcoRII 564 CCAGG ^CC[AT]GG 1214 CCTGG EcoT22I 1870 ATGCAT ATGCA^T 2233 ATGCAT FauI 2057 CCCGC CCCGC(4/6) 2458 GCGGG HindIII 12 AAGCTT A^AGCTT 2312 AAGCTT MaeI 653 CTAG C^TAG 1171 CTAG MboI 677 GATC ^GATC 1598 GATC MflI 676 AGATCT [GA]^GATC[CT] 1597 AGATCC MluNI 444 TGGCCA TGG^CCA 1829 TGGCCA MscI 444 TGGCCA TGG^CCA 1829 TGGCCA MvaI 564 CCAGG CC^[AT]GG 1214 CCTGG NdeII 677 GATC ^GATC 1598 GATC NsiI 1870 ATGCAT ATGCA^T 2233 ATGCAT NspI 1985 GCATGT [GA]CATG^[CT] 2235 GCATGT PpuMI 1408 GGGACCC [GA]G^G[AT]CC[CT] 1998 AGGTCCT PshBI 787 ATTAAT AT^TAAT 882 ATTAAT PvuII 2283 CAGCTG CAG^CTG 2303 CAGCTG Sau3AI 677 GATC ^GATC 1598 GATC ScrFI 564 CCAGG CC^[ACGT]GG 1214 CCTGG SspBI 1787 TGTACA T^GTACA 2064 TGTACA XhoII 676 AGATCT [GA]^GATC[CT] 1597 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI BsmI Bsp1286I BsrDI BsrI BstF5I Cac8I CfoI Cfr13I DdeI EcoT14I Fnu4HI FokI HaeII HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI MaeIII MboII MnlI MseI MslI MspA1I MwoI NlaIII NlaIV PleI RsaI Sau96I SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I