(Circular) (Six-base) MAPSORT of: pFN21AE1799.seq Check: 77 from: 1 to: 7234 pFN21AE1799 7234 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5044 278 Size: 53 83 186 936 3508 2468 Fragments arranged by size: 3508 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2639 4577 1937 Size: 702 1938 4594 Fragments arranged by size: 4594 1938 702 Acc65I G'GTAC_C Cuts at: 4555 4555 Size: 7234 AclI AA'CG_TT Cuts at: 1563 5361 5734 1563 Size: 3798 373 3063 Fragments arranged by size: 3798 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3078 3993 5297 6681 6949 1632 Size: 1446 915 1304 1384 268 1917 Fragments arranged by size: 1917 1446 1384 1304 915 268 AfeI AGC'GCT Cuts at: 2669 4115 2669 Size: 1446 5788 AflII C'TTAA_G Cuts at: 820 1017 4034 820 Size: 197 3017 4020 Fragments arranged by size: 4020 3017 197 AflIII A'CryG_T Cuts at: 1203 6133 7110 1203 Size: 4930 977 1327 Fragments arranged by size: 4930 1327 977 AgeI A'CCGG_T Cuts at: 6065 6065 Size: 7234 AhdI GACnn_n'nnGTC Cuts at: 3420 5963 3420 Size: 2543 4691 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4408 1184 Size: 3224 4010 AlwNI CAG_nnn'CTG Cuts at: 2162 3230 4977 6549 2162 Size: 1068 1747 1572 2847 Fragments arranged by size: 2847 1747 1572 1068 ApaLI G'TGCA_C Cuts at: 5291 6447 5291 Size: 1156 6078 ApoI r'AATT_y Cuts at: 1493 2600 2807 3044 3356 3710 4132 4543 4769 Size: 1107 207 237 312 354 422 411 226 Cuts at: 4769 4921 7030 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1107 422 411 354 312 237 226 207 152 AseI AT'TA_AT Cuts at: 160 1032 2793 2888 5788 160 Size: 872 1761 95 2900 1606 Fragments arranged by size: 2900 1761 1606 872 95 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7234 AvaI C'yCGr_G Cuts at: 1870 1943 4559 1870 Size: 73 2616 4545 Fragments arranged by size: 4545 2616 73 BamHI G'GATC_C Cuts at: 4564 4931 4564 Size: 367 6867 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4325 4555 6010 618 Size: 325 217 627 2538 230 1455 1842 Fragments arranged by size: 2538 1842 1455 627 325 230 217 BanII G_rGCy'C Cuts at: 1391 3337 3536 4553 1391 Size: 1946 199 1017 4072 Fragments arranged by size: 4072 1946 1017 199 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2343 2358 928 Size: 971 444 15 5804 Fragments arranged by size: 5804 971 444 15 BbvCI CC'TCA_GC Cuts at: 4306 4306 Size: 7234 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5425 5459 1903 Size: 34 3488 34 3678 Fragments arranged by size: 3678 3488 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 4206 5126 6336 3803 Size: 403 920 1210 4701 Fragments arranged by size: 4701 1210 920 403 BclI T'GATC_A Cuts at: 1548 1548 Size: 7234 BfrBI ATG'CAT Cuts at: 3877 4240 3877 Size: 363 6871 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5845 136 Size: 107 122 71 631 605 4173 1525 Fragments arranged by size: 4173 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2681 7229 2681 Size: 4548 2686 BlpI GC'TnA_GC Cuts at: 2823 4651 2823 Size: 1828 5406 Bme1580I G_kGCm'C Cuts at: 1790 5295 6451 1790 Size: 3505 1156 2573 Fragments arranged by size: 3505 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7234 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3582 3824 4965 Size: 416 53 83 399 24 2165 242 1141 Cuts at: 4965 5923 442 Size: 958 1753 Fragments arranged by size: 2165 1753 1141 958 416 399 242 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7234 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 3674 4012 5894 1239 Size: 822 1613 338 1882 2579 Fragments arranged by size: 2579 1882 1613 822 338 Bpu10I CC'TnA_GC Cuts at: 4306 4306 Size: 7234 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3231 3682 4176 5359 6224 6522 3231 Size: 451 494 1183 865 298 3943 Fragments arranged by size: 3943 1183 865 494 451 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2371 5897 882 Size: 617 872 3526 2219 Fragments arranged by size: 3526 2219 872 617 BsaAI yAC'GTr Cuts at: 493 1204 5025 493 Size: 711 3821 2702 Fragments arranged by size: 3821 2702 711 BsaBI GATnn'nnATC Cuts at: 2686 7228 2686 Size: 4542 2692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5041 5423 6058 275 Size: 53 83 186 936 3508 382 635 1451 Fragments arranged by size: 3508 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5667 6065 6339 6486 6897 5667 Size: 398 274 147 411 6004 Fragments arranged by size: 6004 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3462 3492 3523 3553 1178 Size: 30 165 30 2059 30 31 30 4859 Fragments arranged by size: 4859 2059 165 31 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2062 2264 2394 2949 3135 3662 1186 Size: 876 202 130 555 186 527 4758 Fragments arranged by size: 4758 876 555 527 202 186 130 BseYI C'CCAG_C Cuts at: 4116 6437 6933 4116 Size: 2321 496 4417 Fragments arranged by size: 4417 2321 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3168 1163 Size: 573 1432 5229 Fragments arranged by size: 5229 1432 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4684 5445 6473 1540 Size: 275 186 2683 761 1028 2301 Fragments arranged by size: 2683 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3660 4192 5295 5380 6451 3660 Size: 532 1103 85 1071 4443 Fragments arranged by size: 4443 1103 1071 532 85 BsmI GAATG_Cn' Cuts at: 2123 3575 4063 4745 4838 2123 Size: 1452 488 682 93 4519 Fragments arranged by size: 4519 1452 682 488 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3337 3536 3660 4192 4553 5295 5380 Size: 399 1547 199 124 532 361 742 85 Cuts at: 5380 6451 1391 Size: 1071 2174 Fragments arranged by size: 2174 1547 1071 742 532 399 361 199 124 85 BspEI T'CCGG_A Cuts at: 6897 6897 Size: 7234 BspHI T'CATG_A Cuts at: 5123 6853 5123 Size: 1730 5504 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4589 844 Size: 3745 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5121 1394 Size: 429 3298 3507 Fragments arranged by size: 3507 3298 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2128 2320 3370 3430 3679 3808 5729 Size: 1183 880 192 1050 60 249 129 1921 Cuts at: 5729 5903 65 Size: 174 1396 Fragments arranged by size: 1921 1396 1183 1050 880 249 192 174 129 60 BsrFI r'CCGG_y Cuts at: 1700 1810 3375 5878 6065 1700 Size: 110 1565 2503 187 2869 Fragments arranged by size: 2869 2503 1565 187 110 BsrGI T'GTAC_A Cuts at: 96 3792 4069 96 Size: 3696 277 3261 Fragments arranged by size: 3696 3261 277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7234 BssSI C'ACGA_G Cuts at: 5294 6306 5294 Size: 1012 6222 BstAPI GCAn_nnn'nTGC Cuts at: 794 3636 794 Size: 2842 4392 BstBI TT'CG_AA Cuts at: 6038 6038 Size: 7234 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7234 BstYI r'GATC_y Cuts at: 1972 2681 3602 4564 4931 5316 5333 6785 6871 Size: 709 921 962 367 385 17 1452 86 Cuts at: 6871 6883 7229 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 962 921 709 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3022 3022 Size: 7234 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6682 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6495 Fragments arranged by size: 6495 683 56 BtsI GCAGTG_nn' Cuts at: 4104 4750 5544 5564 4104 Size: 646 794 20 5774 Fragments arranged by size: 5774 794 646 20 ClaI AT'CG_AT Cuts at: 1554 2394 1554 Size: 840 6394 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7199 DraI TTT'AAA Cuts at: 3084 4538 3084 Size: 1454 5780 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6241 809 Size: 507 4925 1802 Fragments arranged by size: 4925 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2449 3834 4681 Size: 54 1425 50 275 18 619 1385 847 Cuts at: 4681 5570 7199 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1385 889 847 619 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4681 1537 Size: 275 2869 4090 Fragments arranged by size: 4090 2869 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3336 5164 1353 Size: 1983 1828 3423 Fragments arranged by size: 3423 1983 1828 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5821 6195 6341 387 Size: 1064 138 4232 374 146 1280 Fragments arranged by size: 4232 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 3078 3674 3993 4012 5297 5894 Size: 393 429 1017 596 319 19 1285 597 Cuts at: 5894 6681 6949 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1285 1017 787 597 596 429 393 319 268 19 EcoNI CCTnn'n_nnAGG Cuts at: 2232 2232 Size: 7234 EcoO109I rG'GnC_Cy Cuts at: 3414 4004 3414 Size: 590 6644 EcoRI G'AATT_C Cuts at: 3710 4132 4543 4921 3710 Size: 422 411 378 6023 Fragments arranged by size: 6023 422 411 378 EcoRV GAT'ATC Cuts at: 2392 2392 Size: 7234 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2397 2689 2397 Size: 292 6942 FspI TGC'GCA Cuts at: 5740 5740 Size: 7234 HaeII r_GCGC'y Cuts at: 1678 2671 4117 4470 6381 1678 Size: 993 1446 353 1911 2531 Fragments arranged by size: 2531 1911 1446 993 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2128 2160 2896 2928 3406 Size: 32 135 32 556 32 736 32 478 Cuts at: 3406 3438 4204 4236 5875 5907 5949 5981 1373 Size: 32 766 32 1639 32 42 32 2626 Fragments arranged by size: 2626 1639 766 736 556 478 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2083 2640 4089 4578 4824 669 Size: 988 281 145 557 1449 489 246 3079 Fragments arranged by size: 3079 1449 988 557 489 281 246 145 HindIII A'AGCT_T Cuts at: 748 2017 4317 748 Size: 1269 2300 3665 Fragments arranged by size: 3665 2300 1269 HpaI GTT'AAC Cuts at: 4089 4824 4089 Size: 735 6499 KpnI G_GTAC'C Cuts at: 4559 4559 Size: 7234 MfeI C'AATT_G Cuts at: 4833 4833 Size: 7234 MluI A'CGCG_T Cuts at: 7110 7110 Size: 7234 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2176 2267 2642 3491 3592 3728 6348 6532 Size: 871 91 375 849 101 136 2620 184 Cuts at: 6532 1305 Size: 2007 Fragments arranged by size: 2620 2007 871 849 375 184 136 101 91 MscI TGG'CCA Cuts at: 10 64 1489 1832 2451 3836 10 Size: 54 1425 343 619 1385 3408 Fragments arranged by size: 3408 1425 1385 619 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2030 2721 4390 4418 5192 5551 Size: 648 843 21 691 1669 28 774 359 Cuts at: 5551 5710 518 Size: 159 2042 Fragments arranged by size: 2042 1669 843 774 691 648 359 159 28 21 MspA1I CmG'CkG Cuts at: 1606 4065 4290 4310 4650 5327 6475 6720 1606 Size: 2459 225 20 340 677 1148 245 2120 Fragments arranged by size: 2459 2120 1148 677 340 245 225 20 NaeI GCC'GGC Cuts at: 3377 3377 Size: 7234 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6682 NdeI CA'TA_TG Cuts at: 387 387 Size: 7234 NgoMIV G'CCGG_C Cuts at: 3375 3375 Size: 7234 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7234 NotI GC'GGCC_GC Cuts at: 4681 4681 Size: 7234 NsiI A_TGCA'T Cuts at: 3879 4242 3879 Size: 363 6871 NspI r_CATG'y Cuts at: 1132 1229 3994 4244 4592 6137 1132 Size: 97 2765 250 348 1545 2229 Fragments arranged by size: 2765 2229 1545 348 250 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7234 PciI A'CATG_T Cuts at: 6133 6133 Size: 7234 PflMI CCAn_nnn'nTGG Cuts at: 1108 2755 1108 Size: 1647 5587 PmeI GTTT'AAAC Cuts at: 4538 4538 Size: 7234 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5303 5335 6816 6848 5303 Size: 32 1481 32 5689 Fragments arranged by size: 5689 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3414 4004 3414 Size: 590 6644 PshAI GACnn'nnGTC Cuts at: 1613 3366 1613 Size: 1753 5481 PsiI TTA'TAA Cuts at: 4804 4804 Size: 7234 PstI C_TGCA'G Cuts at: 830 4131 4586 830 Size: 3301 455 3478 Fragments arranged by size: 3478 3301 455 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7234 PvuII CAG'CTG Cuts at: 4290 4310 4290 Size: 20 7214 SalI G'TCGA_C Cuts at: 1936 2638 4576 1936 Size: 702 1938 4594 Fragments arranged by size: 4594 1938 702 SanDI GG'GwC_CC Cuts at: 3414 3414 Size: 7234 SbfI CC_TGCA'GG Cuts at: 4586 4586 Size: 7234 ScaI AGT'ACT Cuts at: 2614 5482 7194 2614 Size: 2868 1712 2654 Fragments arranged by size: 2868 2654 1712 SfcI C'TryA_G Cuts at: 826 1045 2533 2869 4127 4582 5717 6398 6589 Size: 219 1488 336 1258 455 1135 681 191 Cuts at: 6589 826 Size: 1471 Fragments arranged by size: 1488 1471 1258 1135 681 455 336 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7234 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7234 SmaI CCC'GGG Cuts at: 1872 4561 1872 Size: 2689 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3246 3661 4034 4191 5338 6239 Size: 197 926 1303 415 373 157 1147 901 Cuts at: 6239 6501 820 Size: 262 1553 Fragments arranged by size: 1553 1303 1147 926 901 415 373 262 197 157 SnaBI TAC'GTA Cuts at: 493 5025 493 Size: 4532 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7234 SphI G_CATG'C Cuts at: 1132 4592 1132 Size: 3460 3774 SspI AAT'ATT Cuts at: 5 52 5158 5 Size: 47 5106 2081 Fragments arranged by size: 5106 2081 47 StuI AGG'CCT Cuts at: 1517 4513 1517 Size: 2996 4238 StyI C'CwwG_G Cuts at: 513 1065 2108 3544 4176 4673 513 Size: 552 1043 1436 632 497 3074 Fragments arranged by size: 3074 1436 1043 632 552 497 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5261 5278 5431 5616 6061 325 Size: 1255 3681 17 153 185 445 1498 Fragments arranged by size: 3681 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2612 3792 3907 Size: 274 80 33 51 1443 635 1180 115 Cuts at: 3907 4069 4292 5480 7192 96 Size: 162 223 1188 1712 138 Fragments arranged by size: 1712 1443 1188 1180 635 274 223 162 138 115 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3523 3555 3816 3848 7194 7226 3523 Size: 32 261 32 3346 32 3531 Fragments arranged by size: 3531 3346 261 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4447 1316 Size: 3131 4103 XbaI T'CTAG_A Cuts at: 4570 4570 Size: 7234 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2192 2192 Size: 7234 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7234 XmaI C'CCGG_G Cuts at: 1870 4559 1870 Size: 2689 4545 XmnI GAAnn'nnTTC Cuts at: 5363 5363 Size: 7234 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5042 276 Size: 53 83 186 936 3508 2468 Fragments arranged by size: 3508 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI