################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI AspEI AspI AvrII BalI BamHI BbeI BbrPI BbvCI BfrI BglI BglII BlnI BsaAI BsaI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HgaI HincII HindII HpaI KasI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 481 GACGTC GACGT^C Acc65I 422 GGTACC G^GTACC AccI 63 GTCTAC GT^[AC][GT]AC AcyI 481 GACGTC G[GA]^CG[CT]C AlwNI 127 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 709 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 422 GGTACC G^GTACC AvaI 178 CCCGAG C^[CT]CG[GA]G AviII 98 TGCGCA TGC^GCA BanII 175 GAGCCC G[GA]GC[CT]^C BciVI 1085 GGATAC GTATCC(6/5) BclI 870 TGATCA T^GATCA BcnI 192 CCCGG CC^[GC]GG BsaBI 201 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 481 GACGTC G[GA]^CG[CT]C BseRI 276 GAGGAG GAGGAG(10/8) BsmAI 484 GTCTC GTCTC(1/5) BsmBI 483 CGTCTC CGTCTC(1/5) BsmI 501 GAATGC GAATGC(1/-1) BsoBI 178 CCCGAG C^[CT]CG[GA]G BspHI 705 TCATGA T^CATGA BsrDI 490 GCAATG GCAATG(2/0) BsrFI 974 ACCGGC [GA]^CCGG[CT] Cfr10I 974 ACCGGC [GA]^CCGG[CT] DraI 742 TTTAAA TTT^AAA DraII 1169 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 120 CCGCGG C^C[GA][CT]GG EaeI 257 CGGCCG [CT]^GGCC[GA] EagI 257 CGGCCG C^GGCCG EarI 286 GAAGAG CTCTTC(1/4) EclXI 257 CGGCCG C^GGCCG Eco52I 257 CGGCCG C^GGCCG EcoO109I 1169 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 299 CCAAGG C^C[AT][AT]GG FbaI 870 TGATCA T^GATCA FspI 98 TGCGCA TGC^GCA Hin1I 481 GACGTC G[GA]^CG[CT]C HindIII 750 AAGCTT A^AGCTT KpnI 422 GGTACC GGTAC^C Ksp632I 286 GAAGAG CTCTTC(1/4) KspI 120 CCGCGG CCGC^GG MamI 201 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 4 CACTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 120 CCGCGG C[AC]G^C[GT]G NciI 192 CCCGG CC^[GC]GG NotI 256 GCGGCCGC GC^GGCCGC PpuMI 1169 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 953 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 69 CTGCAG CTGCA^G RcaI 705 TCATGA T^CATGA SacII 120 CCGCGG CCGC^GG ScaI 290 AGTACT AGT^ACT SphI 1129 GCATGC GCATG^C StyI 299 CCAAGG C^C[AT][AT]GG TfiI 594 GATTC G^A[AT]TC XcmI 408 CCAGGTGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 709 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Aor51HI 19 AGCGCT AGC^GCT 629 AGCGCT AspHI 393 GTGCTC G[AT]GC[AT]^C 412 GTGCTC AvaII 87 GGACC G^G[AT]CC 1170 GGTCC BanI 422 GGTACC G^G[CT][GA]CC 847 GGCACC BbsI 163 GAAGAC GAAGAC(2/6) 912 GAAGAC BcgI 71 GCAGCACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 101 GCAAGAGGATCG BlpI 15 GCTGAGC GC^T[ACGT]AGC 54 GCTCAGC Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC 54 GCTCAGC BpuAI 163 GAAGAC GAAGAC(2/6) 912 GAAGAC BsgI 768 CTGCAC GTGCAG(16/14) 1021 GTGCAG BsiEI 47 CGGTCG CG[GA][CT]^CG 257 CGGCCG BsiHKAI 393 GTGCTC G[AT]GC[AT]^C 412 GTGCTC BspMI 67 ACCTGC ACCTGC(4/8) 835 GCAGGT BstAPI 386 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 826 GCAAGATTTGC BstYI 128 AGATCC [GA]^GATC[CT] 1068 GGATCT CelII 15 GCTGAGC GC^T[ACGT]AGC 54 GCTCAGC Cfr13I 87 GGACC G^G[ACGT]CC 1170 GGTCC Eco47III 19 AGCGCT AGC^GCT 629 AGCGCT FauI 438 CCCGC CCCGC(4/6) 122 GCGGG HaeII 19 AGCGCT [GA]GCGC^[CT] 629 AGCGCT HaeIII 258 GGCC GG^CC 1002 GGCC MaeII 482 ACGT A^CGT 1041 ACGT MflI 128 AGATCC [GA]^GATC[CT] 1068 GGATCT MseI 613 TTAA T^TAA 743 TTAA NspI 320 ACATGC [GA]CATG^[CT] 1129 GCATGC PleI 533 GAGTC GAGTC(4/5) 958 GAGTC Sau96I 87 GGACC G^G[ACGT]CC 1170 GGTCC SmlI 598 CTCAAG C^T[CT][GA]AG 955 CTTGAG Tru9I 613 TTAA T^TAA 743 TTAA Tsp45I 43 GTCAC ^GT[GC]AC 960 GTCAC XhoII 128 AGATCC [GA]^GATC[CT] 1068 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI DdeI DpnII EcoRII Fnu4HI FokI HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI SfcI TaqI TseI Tsp509I TspRI TthHB8I