(Circular) (Six-base) MAPSORT of: pFN21AE1906.seq Check: 7116 from: 1 to: 6031 pFN21AE1906 6031 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2490 3841 278 Size: 53 83 186 936 954 1351 2468 Fragments arranged by size: 2468 1351 954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2069 3374 1937 Size: 132 1305 4594 Fragments arranged by size: 4594 1305 132 Acc65I G'GTAC_C Cuts at: 2427 3352 2427 Size: 925 5106 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2168 2452 4094 5478 5746 1632 Size: 536 284 1642 1384 268 1917 Fragments arranged by size: 1917 1642 1384 536 284 268 AfeI AGC'GCT Cuts at: 2026 2636 2026 Size: 610 5421 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 4930 5907 1203 Size: 3727 977 1327 Fragments arranged by size: 3727 1327 977 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 2013 1166 Size: 847 5184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6031 AlwNI CAG_nnn'CTG Cuts at: 2137 3774 5346 2137 Size: 1637 1572 2822 Fragments arranged by size: 2822 1637 1572 ApaLI G'TGCA_C Cuts at: 4088 5244 4088 Size: 1156 4875 ApoI r'AATT_y Cuts at: 1493 3340 3566 3718 5827 1493 Size: 1847 226 152 2109 1697 Fragments arranged by size: 2109 1847 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 2183 3356 1870 Size: 73 240 1173 4545 Fragments arranged by size: 4545 1173 240 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2766 2799 2766 Size: 33 5998 BamHI G'GATC_C Cuts at: 3361 3728 3361 Size: 367 5664 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2427 2852 3352 4807 618 Size: 325 217 627 640 425 500 1455 1842 Fragments arranged by size: 1842 1455 640 627 500 425 325 217 BanII G_rGCy'C Cuts at: 1391 2184 3350 1391 Size: 793 1166 4072 Fragments arranged by size: 4072 1166 793 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2175 2924 928 Size: 971 276 749 4035 Fragments arranged by size: 4035 971 749 276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2065 2095 2099 2129 4222 Size: 34 50 34 44 30 4 30 2093 Cuts at: 4222 4256 1903 Size: 34 3678 Fragments arranged by size: 3678 2093 50 44 34 34 34 30 30 4 BciVI GTATCCnnnnn_n' Cuts at: 3084 3923 5133 3084 Size: 839 1210 3982 Fragments arranged by size: 3982 1210 839 BclI T'GATC_A Cuts at: 1548 2875 1548 Size: 1327 4704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4642 136 Size: 107 122 71 631 605 2970 1525 Fragments arranged by size: 2970 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6026 6026 Size: 6031 BlpI GC'TnA_GC Cuts at: 2021 2060 3448 2021 Size: 39 1388 4604 Fragments arranged by size: 4604 1388 39 Bme1580I G_kGCm'C Cuts at: 1790 4092 5248 1790 Size: 2302 1156 2573 Fragments arranged by size: 2573 2302 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6031 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2354 3762 4720 Size: 416 53 83 399 24 937 1408 958 Cuts at: 4720 442 Size: 1753 Fragments arranged by size: 1753 1408 958 937 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2386 3150 4691 1239 Size: 983 164 764 1541 2579 Fragments arranged by size: 2579 1541 983 764 164 Bpu10I CC'TnA_GC Cuts at: 2138 2178 3179 2138 Size: 40 1001 4990 Fragments arranged by size: 4990 1001 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2588 2981 4156 5021 5319 2588 Size: 393 1175 865 298 3300 Fragments arranged by size: 3300 1175 865 393 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4694 882 Size: 617 3195 2219 Fragments arranged by size: 3195 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3822 493 Size: 711 2618 2702 Fragments arranged by size: 2702 2618 711 BsaBI GATnn'nnATC Cuts at: 2210 6025 2210 Size: 3815 2216 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2487 3838 4220 4855 Size: 53 83 186 936 954 1351 382 635 Cuts at: 4855 275 Size: 1451 Fragments arranged by size: 1451 1351 954 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4464 4862 5136 5283 5694 4464 Size: 398 274 147 411 4801 Fragments arranged by size: 4801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5806 Fragments arranged by size: 5806 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2296 1186 Size: 1110 4921 BseYI C'CCAG_C Cuts at: 2631 5234 5730 2631 Size: 2603 496 2932 Fragments arranged by size: 2932 2603 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2758 3047 1163 Size: 573 1022 289 4147 Fragments arranged by size: 4147 1022 573 289 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 2265 3481 4242 5270 1540 Size: 275 186 54 210 1216 761 1028 2301 Fragments arranged by size: 2301 1216 1028 761 275 210 186 54 BsiHKAI G_wGCw'C Cuts at: 2402 2421 4092 4177 5248 2402 Size: 19 1671 85 1071 3185 Fragments arranged by size: 3185 1671 1071 85 19 BsmI GAATG_Cn' Cuts at: 2512 3542 3635 2512 Size: 1030 93 4908 Fragments arranged by size: 4908 1030 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2494 2494 Size: 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2402 2421 3350 4092 4177 5248 Size: 399 394 218 19 929 742 85 1071 Cuts at: 5248 1391 Size: 2174 Fragments arranged by size: 2174 1071 929 742 399 394 218 85 19 BspEI T'CCGG_A Cuts at: 5694 5694 Size: 6031 BspHI T'CATG_A Cuts at: 2710 3920 5650 2710 Size: 1210 1730 3091 Fragments arranged by size: 3091 1730 1210 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2081 2831 3386 844 Size: 1237 750 555 3489 Fragments arranged by size: 3489 1237 750 555 BsrBI CCG'CTC Cuts at: 1394 1823 3918 1394 Size: 429 2095 3507 Fragments arranged by size: 3507 2095 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2502 4526 4700 65 Size: 1183 1254 2024 174 1396 Fragments arranged by size: 2024 1396 1254 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2979 4675 4862 1700 Size: 110 1169 1696 187 2869 Fragments arranged by size: 2869 1696 1169 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6031 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6031 BssSI C'ACGA_G Cuts at: 4091 5103 4091 Size: 1012 5019 BstAPI GCAn_nnn'nTGC Cuts at: 794 2397 2837 794 Size: 1603 440 3988 Fragments arranged by size: 3988 1603 440 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstYI r'GATC_y Cuts at: 1972 2133 3073 3361 3728 4113 4130 5582 5668 Size: 161 940 288 367 385 17 1452 86 Cuts at: 5668 5680 6026 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 940 385 367 346 288 161 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2125 513 Size: 552 1060 4419 Fragments arranged by size: 4419 1060 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5292 Fragments arranged by size: 5292 683 56 BtsI GCAGTG_nn' Cuts at: 3547 4341 4361 3547 Size: 794 20 5217 Fragments arranged by size: 5217 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 2749 3335 2749 Size: 586 5445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5038 809 Size: 507 3722 1802 Fragments arranged by size: 3722 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 3478 4367 Size: 54 1425 50 275 18 432 1216 889 Cuts at: 4367 5996 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1216 889 432 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2262 3478 1537 Size: 275 450 1216 4090 Fragments arranged by size: 4090 1216 450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 3961 1353 Size: 933 1675 3423 Fragments arranged by size: 3423 1675 933 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2998 4618 4992 5138 387 Size: 1064 138 1409 1620 374 146 1280 Fragments arranged by size: 1620 1409 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2222 2386 2452 3150 4094 4691 Size: 393 536 54 164 66 698 944 597 Cuts at: 4691 5478 5746 1239 Size: 787 268 1524 Fragments arranged by size: 1524 944 787 698 597 536 393 268 164 66 54 EcoO109I rG'GnC_Cy Cuts at: 3175 3175 Size: 6031 EcoRI G'AATT_C Cuts at: 3340 3718 3340 Size: 378 5653 FspI TGC'GCA Cuts at: 2105 4537 2105 Size: 2432 3599 HaeII r_GCGC'y Cuts at: 1678 2028 2638 5178 1678 Size: 350 610 2540 2531 Fragments arranged by size: 2540 2531 610 350 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 2472 2504 2606 Size: 32 135 32 454 32 414 32 102 Cuts at: 2606 2638 2840 2872 2911 2943 4672 4704 4746 Size: 32 202 32 39 32 1729 32 42 Cuts at: 4746 4778 1373 Size: 32 2626 Fragments arranged by size: 2626 1729 454 414 202 135 102 42 39 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3375 3621 669 Size: 988 281 1437 246 3079 Fragments arranged by size: 3079 1437 988 281 246 HindIII A'AGCT_T Cuts at: 748 2755 748 Size: 2007 4024 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KpnI G_GTAC'C Cuts at: 2431 3356 2431 Size: 925 5106 MfeI C'AATT_G Cuts at: 3630 3630 Size: 6031 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2651 5145 5329 1305 Size: 1346 2494 184 2007 Fragments arranged by size: 2494 2007 1346 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4209 Fragments arranged by size: 4209 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2013 3989 4348 4507 518 Size: 648 847 1976 359 159 2042 Fragments arranged by size: 2042 1976 847 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2127 3447 4124 5272 5517 1606 Size: 521 1320 677 1148 245 2120 Fragments arranged by size: 2120 1320 1148 677 521 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5479 NdeI CA'TA_TG Cuts at: 387 387 Size: 6031 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 2262 3478 2262 Size: 1216 4815 NspI r_CATG'y Cuts at: 1132 1229 2329 3138 3389 4934 1132 Size: 97 1100 809 251 1545 2229 Fragments arranged by size: 2229 1545 1100 809 251 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6031 PciI A'CATG_T Cuts at: 4930 4930 Size: 6031 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6031 PfoI T'CCnGG_A Cuts at: 2238 2238 Size: 6031 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5613 5645 4100 Size: 32 1481 32 4486 Fragments arranged by size: 4486 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3175 3175 Size: 6031 PshAI GACnn'nnGTC Cuts at: 1613 2962 1613 Size: 1349 4682 PsiI TTA'TAA Cuts at: 3601 3601 Size: 6031 PstI C_TGCA'G Cuts at: 830 2078 3383 830 Size: 1248 1305 3478 Fragments arranged by size: 3478 1305 1248 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 SacII CC_GC'GG Cuts at: 2128 2128 Size: 6031 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SbfI CC_TGCA'GG Cuts at: 3383 3383 Size: 6031 ScaI AGT'ACT Cuts at: 2297 4279 5991 2297 Size: 1982 1712 2337 Fragments arranged by size: 2337 1982 1712 SfcI C'TryA_G Cuts at: 826 1045 2074 2455 2953 3379 4514 5195 5386 Size: 219 1029 381 498 426 1135 681 191 Cuts at: 5386 826 Size: 1471 Fragments arranged by size: 1471 1135 1029 681 498 426 381 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 3358 1872 Size: 1486 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2603 2960 4135 5036 5298 820 Size: 197 926 660 357 1175 901 262 1553 Fragments arranged by size: 1553 1175 926 901 660 357 262 197 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 3138 3389 1132 Size: 2006 251 3774 Fragments arranged by size: 3774 2006 251 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6031 StyI C'CwwG_G Cuts at: 513 1065 2304 3470 513 Size: 552 1239 1166 3074 Fragments arranged by size: 3074 1239 1166 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3295 4058 4075 4228 4413 4858 325 Size: 1255 1715 763 17 153 185 445 1498 Fragments arranged by size: 1715 1498 1255 763 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 4277 5989 Size: 274 80 33 51 1443 318 1982 1712 Cuts at: 5989 96 Size: 138 Fragments arranged by size: 1982 1712 1443 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2977 3009 3065 3097 5991 6023 2977 Size: 32 56 32 2894 32 2985 Fragments arranged by size: 2985 2894 56 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2420 2420 Size: 6031 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 3356 1870 Size: 1486 4545 XmnI GAAnn'nnTTC Cuts at: 2718 4160 2718 Size: 1442 4589 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2488 3839 276 Size: 53 83 186 936 954 1351 2468 Fragments arranged by size: 2468 1351 954 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI