Restriction map for
pFN21AE1906
(Circular) (Six-base) MAPSORT of: pFN21AE1906.seq Check: 7116 from: 1 to: 6031
pFN21AE1906 6031 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2490 3841 278
Size: 53 83 186 936 954 1351 2468
Fragments arranged by size:
2468 1351 954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2069 3374 1937
Size: 132 1305 4594
Fragments arranged by size:
4594 1305 132
Acc65I G'GTAC_C
Cuts at: 2427 3352 2427
Size: 925 5106
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2168 2452 4094 5478 5746 1632
Size: 536 284 1642 1384 268 1917
Fragments arranged by size:
1917 1642 1384 536 284 268
AfeI AGC'GCT
Cuts at: 2026 2636 2026
Size: 610 5421
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 4930 5907 1203
Size: 3727 977 1327
Fragments arranged by size:
3727 1327 977
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 2013 1166
Size: 847 5184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6031
AlwNI CAG_nnn'CTG
Cuts at: 2137 3774 5346 2137
Size: 1637 1572 2822
Fragments arranged by size:
2822 1637 1572
ApaLI G'TGCA_C
Cuts at: 4088 5244 4088
Size: 1156 4875
ApoI r'AATT_y
Cuts at: 1493 3340 3566 3718 5827 1493
Size: 1847 226 152 2109 1697
Fragments arranged by size:
2109 1847 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 2183 3356 1870
Size: 73 240 1173 4545
Fragments arranged by size:
4545 1173 240 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2766 2799 2766
Size: 33 5998
BamHI G'GATC_C
Cuts at: 3361 3728 3361
Size: 367 5664
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2427 2852 3352 4807 618
Size: 325 217 627 640 425 500 1455 1842
Fragments arranged by size:
1842 1455 640 627 500 425 325 217
BanII G_rGCy'C
Cuts at: 1391 2184 3350 1391
Size: 793 1166 4072
Fragments arranged by size:
4072 1166 793
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2175 2924 928
Size: 971 276 749 4035
Fragments arranged by size:
4035 971 749 276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2065 2095 2099 2129 4222
Size: 34 50 34 44 30 4 30 2093
Cuts at: 4222 4256 1903
Size: 34 3678
Fragments arranged by size:
3678 2093 50 44 34 34 34 30
30 4
BciVI GTATCCnnnnn_n'
Cuts at: 3084 3923 5133 3084
Size: 839 1210 3982
Fragments arranged by size:
3982 1210 839
BclI T'GATC_A
Cuts at: 1548 2875 1548
Size: 1327 4704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4642 136
Size: 107 122 71 631 605 2970 1525
Fragments arranged by size:
2970 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6026 6026
Size: 6031
BlpI GC'TnA_GC
Cuts at: 2021 2060 3448 2021
Size: 39 1388 4604
Fragments arranged by size:
4604 1388 39
Bme1580I G_kGCm'C
Cuts at: 1790 4092 5248 1790
Size: 2302 1156 2573
Fragments arranged by size:
2573 2302 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6031
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2354 3762 4720
Size: 416 53 83 399 24 937 1408 958
Cuts at: 4720 442
Size: 1753
Fragments arranged by size:
1753 1408 958 937 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2386 3150 4691 1239
Size: 983 164 764 1541 2579
Fragments arranged by size:
2579 1541 983 764 164
Bpu10I CC'TnA_GC
Cuts at: 2138 2178 3179 2138
Size: 40 1001 4990
Fragments arranged by size:
4990 1001 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2588 2981 4156 5021 5319 2588
Size: 393 1175 865 298 3300
Fragments arranged by size:
3300 1175 865 393 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4694 882
Size: 617 3195 2219
Fragments arranged by size:
3195 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3822 493
Size: 711 2618 2702
Fragments arranged by size:
2702 2618 711
BsaBI GATnn'nnATC
Cuts at: 2210 6025 2210
Size: 3815 2216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2487 3838 4220 4855
Size: 53 83 186 936 954 1351 382 635
Cuts at: 4855 275
Size: 1451
Fragments arranged by size:
1451 1351 954 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4464 4862 5136 5283 5694 4464
Size: 398 274 147 411 4801
Fragments arranged by size:
4801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5806
Fragments arranged by size:
5806 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 1186
Size: 1110 4921
BseYI C'CCAG_C
Cuts at: 2631 5234 5730 2631
Size: 2603 496 2932
Fragments arranged by size:
2932 2603 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2758 3047 1163
Size: 573 1022 289 4147
Fragments arranged by size:
4147 1022 573 289
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 2265 3481 4242 5270 1540
Size: 275 186 54 210 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 210 186 54
BsiHKAI G_wGCw'C
Cuts at: 2402 2421 4092 4177 5248 2402
Size: 19 1671 85 1071 3185
Fragments arranged by size:
3185 1671 1071 85 19
BsmI GAATG_Cn'
Cuts at: 2512 3542 3635 2512
Size: 1030 93 4908
Fragments arranged by size:
4908 1030 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2494 2494
Size: 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2402 2421 3350 4092 4177 5248
Size: 399 394 218 19 929 742 85 1071
Cuts at: 5248 1391
Size: 2174
Fragments arranged by size:
2174 1071 929 742 399 394 218 85
19
BspEI T'CCGG_A
Cuts at: 5694 5694
Size: 6031
BspHI T'CATG_A
Cuts at: 2710 3920 5650 2710
Size: 1210 1730 3091
Fragments arranged by size:
3091 1730 1210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2081 2831 3386 844
Size: 1237 750 555 3489
Fragments arranged by size:
3489 1237 750 555
BsrBI CCG'CTC
Cuts at: 1394 1823 3918 1394
Size: 429 2095 3507
Fragments arranged by size:
3507 2095 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2502 4526 4700 65
Size: 1183 1254 2024 174 1396
Fragments arranged by size:
2024 1396 1254 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2979 4675 4862 1700
Size: 110 1169 1696 187 2869
Fragments arranged by size:
2869 1696 1169 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6031
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6031
BssSI C'ACGA_G
Cuts at: 4091 5103 4091
Size: 1012 5019
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2397 2837 794
Size: 1603 440 3988
Fragments arranged by size:
3988 1603 440
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstYI r'GATC_y
Cuts at: 1972 2133 3073 3361 3728 4113 4130 5582 5668
Size: 161 940 288 367 385 17 1452 86
Cuts at: 5668 5680 6026 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 940 385 367 346 288 161
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2125 513
Size: 552 1060 4419
Fragments arranged by size:
4419 1060 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5292
Fragments arranged by size:
5292 683 56
BtsI GCAGTG_nn'
Cuts at: 3547 4341 4361 3547
Size: 794 20 5217
Fragments arranged by size:
5217 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 2749 3335 2749
Size: 586 5445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5038 809
Size: 507 3722 1802
Fragments arranged by size:
3722 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 3478 4367
Size: 54 1425 50 275 18 432 1216 889
Cuts at: 4367 5996 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1216 889 432 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2262 3478 1537
Size: 275 450 1216 4090
Fragments arranged by size:
4090 1216 450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 3961 1353
Size: 933 1675 3423
Fragments arranged by size:
3423 1675 933
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2998 4618 4992 5138 387
Size: 1064 138 1409 1620 374 146 1280
Fragments arranged by size:
1620 1409 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2222 2386 2452 3150 4094 4691
Size: 393 536 54 164 66 698 944 597
Cuts at: 4691 5478 5746 1239
Size: 787 268 1524
Fragments arranged by size:
1524 944 787 698 597 536 393 268
164 66 54
EcoO109I rG'GnC_Cy
Cuts at: 3175 3175
Size: 6031
EcoRI G'AATT_C
Cuts at: 3340 3718 3340
Size: 378 5653
FspI TGC'GCA
Cuts at: 2105 4537 2105
Size: 2432 3599
HaeII r_GCGC'y
Cuts at: 1678 2028 2638 5178 1678
Size: 350 610 2540 2531
Fragments arranged by size:
2540 2531 610 350
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 2472 2504 2606
Size: 32 135 32 454 32 414 32 102
Cuts at: 2606 2638 2840 2872 2911 2943 4672 4704 4746
Size: 32 202 32 39 32 1729 32 42
Cuts at: 4746 4778 1373
Size: 32 2626
Fragments arranged by size:
2626 1729 454 414 202 135 102 42
39 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3375 3621 669
Size: 988 281 1437 246 3079
Fragments arranged by size:
3079 1437 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2755 748
Size: 2007 4024
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KpnI G_GTAC'C
Cuts at: 2431 3356 2431
Size: 925 5106
MfeI C'AATT_G
Cuts at: 3630 3630
Size: 6031
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2651 5145 5329 1305
Size: 1346 2494 184 2007
Fragments arranged by size:
2494 2007 1346 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4209
Fragments arranged by size:
4209 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2013 3989 4348 4507 518
Size: 648 847 1976 359 159 2042
Fragments arranged by size:
2042 1976 847 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2127 3447 4124 5272 5517 1606
Size: 521 1320 677 1148 245 2120
Fragments arranged by size:
2120 1320 1148 677 521 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5479
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6031
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 2262 3478 2262
Size: 1216 4815
NspI r_CATG'y
Cuts at: 1132 1229 2329 3138 3389 4934 1132
Size: 97 1100 809 251 1545 2229
Fragments arranged by size:
2229 1545 1100 809 251 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6031
PciI A'CATG_T
Cuts at: 4930 4930
Size: 6031
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6031
PfoI T'CCnGG_A
Cuts at: 2238 2238
Size: 6031
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5613 5645 4100
Size: 32 1481 32 4486
Fragments arranged by size:
4486 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3175 3175
Size: 6031
PshAI GACnn'nnGTC
Cuts at: 1613 2962 1613
Size: 1349 4682
PsiI TTA'TAA
Cuts at: 3601 3601
Size: 6031
PstI C_TGCA'G
Cuts at: 830 2078 3383 830
Size: 1248 1305 3478
Fragments arranged by size:
3478 1305 1248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
SacII CC_GC'GG
Cuts at: 2128 2128
Size: 6031
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SbfI CC_TGCA'GG
Cuts at: 3383 3383
Size: 6031
ScaI AGT'ACT
Cuts at: 2297 4279 5991 2297
Size: 1982 1712 2337
Fragments arranged by size:
2337 1982 1712
SfcI C'TryA_G
Cuts at: 826 1045 2074 2455 2953 3379 4514 5195 5386
Size: 219 1029 381 498 426 1135 681 191
Cuts at: 5386 826
Size: 1471
Fragments arranged by size:
1471 1135 1029 681 498 426 381 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 3358 1872
Size: 1486 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2603 2960 4135 5036 5298 820
Size: 197 926 660 357 1175 901 262 1553
Fragments arranged by size:
1553 1175 926 901 660 357 262 197
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 3138 3389 1132
Size: 2006 251 3774
Fragments arranged by size:
3774 2006 251
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6031
StyI C'CwwG_G
Cuts at: 513 1065 2304 3470 513
Size: 552 1239 1166 3074
Fragments arranged by size:
3074 1239 1166 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3295 4058 4075 4228 4413 4858 325
Size: 1255 1715 763 17 153 185 445 1498
Fragments arranged by size:
1715 1498 1255 763 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 4277 5989
Size: 274 80 33 51 1443 318 1982 1712
Cuts at: 5989 96
Size: 138
Fragments arranged by size:
1982 1712 1443 318 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2977 3009 3065 3097 5991 6023 2977
Size: 32 56 32 2894 32 2985
Fragments arranged by size:
2985 2894 56 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2420 2420
Size: 6031
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 3356 1870
Size: 1486 4545
XmnI GAAnn'nnTTC
Cuts at: 2718 4160 2718
Size: 1442 4589
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2488 3839 276
Size: 53 83 186 936 954 1351 2468
Fragments arranged by size:
2468 1351 954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI