(Circular) (Six-base) MAPSORT of: pFN21AE1932.seq Check: 7858 from: 1 to: 6115 pFN21AE1932 6115 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3925 278 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3458 1937 Size: 1521 4594 Acc65I G'GTAC_C Cuts at: 3436 3436 Size: 6115 AclI AA'CG_TT Cuts at: 1563 4242 4615 1563 Size: 2679 373 3063 Fragments arranged by size: 3063 2679 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2948 4178 5562 5830 1632 Size: 1316 1230 1384 268 1917 Fragments arranged by size: 1917 1384 1316 1230 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5918 AflIII A'CryG_T Cuts at: 1203 5014 5991 1203 Size: 3811 977 1327 Fragments arranged by size: 3811 1327 977 AgeI A'CCGG_T Cuts at: 4946 4946 Size: 6115 AhdI GACnn_n'nnGTC Cuts at: 4844 4844 Size: 6115 AleI CACnn'nnGTG Cuts at: 1166 2882 1166 Size: 1716 4399 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2055 1184 Size: 871 5244 AlwNI CAG_nnn'CTG Cuts at: 2785 3858 5430 2785 Size: 1073 1572 3470 Fragments arranged by size: 3470 1572 1073 ApaI G_GGCC'C Cuts at: 2325 2325 Size: 6115 ApaLI G'TGCA_C Cuts at: 4172 5328 4172 Size: 1156 4959 ApoI r'AATT_y Cuts at: 1493 2120 2447 2606 3424 3650 3802 5911 1493 Size: 627 327 159 818 226 152 2109 1697 Fragments arranged by size: 2109 1697 818 627 327 226 159 152 AseI AT'TA_AT Cuts at: 160 1032 4669 160 Size: 872 3637 1606 Fragments arranged by size: 3637 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 AvaI C'yCGr_G Cuts at: 1870 1943 3440 1870 Size: 73 1497 4545 Fragments arranged by size: 4545 1497 73 BamHI G'GATC_C Cuts at: 3445 3812 3445 Size: 367 5748 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3436 4891 618 Size: 325 217 627 1649 1455 1842 Fragments arranged by size: 1842 1649 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2053 2325 2506 3434 1391 Size: 662 272 181 928 4072 Fragments arranged by size: 4072 928 662 272 181 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3292 928 Size: 971 1393 3751 Fragments arranged by size: 3751 1393 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2255 2289 4306 4340 1903 Size: 34 318 34 2017 34 3678 Fragments arranged by size: 3678 2017 318 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4007 5217 4007 Size: 1210 4905 BclI T'GATC_A Cuts at: 1548 1548 Size: 6115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2320 4726 136 Size: 107 122 71 631 605 648 2406 1525 Fragments arranged by size: 2406 1525 648 631 605 122 107 71 BglII A'GATC_T Cuts at: 2895 6110 2895 Size: 3215 2900 BlpI GC'TnA_GC Cuts at: 3532 3532 Size: 6115 Bme1580I G_kGCm'C Cuts at: 1790 2325 4176 5332 1790 Size: 535 1851 1156 2573 Fragments arranged by size: 2573 1851 1156 535 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6115 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3846 4804 442 Size: 416 53 83 399 24 2429 958 1753 Fragments arranged by size: 2429 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2067 2276 4775 1239 Size: 828 209 2499 2579 Fragments arranged by size: 2579 2499 828 209 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2153 4240 5105 5403 2153 Size: 2087 865 298 2865 Fragments arranged by size: 2865 2087 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2787 2935 4778 882 Size: 617 1288 148 1843 2219 Fragments arranged by size: 2219 1843 1288 617 148 BsaAI yAC'GTr Cuts at: 493 1204 2771 3906 493 Size: 711 1567 1135 2702 Fragments arranged by size: 2702 1567 1135 711 BsaBI GATnn'nnATC Cuts at: 2295 6109 2295 Size: 3814 2301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3922 4304 4939 275 Size: 53 83 186 936 2389 382 635 1451 Fragments arranged by size: 2389 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2406 4548 4946 5220 5367 5778 2406 Size: 2142 398 274 147 411 2743 Fragments arranged by size: 2743 2142 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5890 Fragments arranged by size: 5890 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6115 BseYI C'CCAG_C Cuts at: 5318 5814 5318 Size: 496 5619 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5542 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3565 4326 5354 1540 Size: 275 186 1564 761 1028 2301 Fragments arranged by size: 2301 1564 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2053 2506 4176 4261 5332 2053 Size: 453 1670 85 1071 2836 Fragments arranged by size: 2836 1670 1071 453 85 BsmI GAATG_Cn' Cuts at: 2775 3626 3719 2775 Size: 851 93 5171 Fragments arranged by size: 5171 851 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2325 2506 3434 4176 4261 5332 Size: 399 263 272 181 928 742 85 1071 Cuts at: 5332 1391 Size: 2174 Fragments arranged by size: 2174 1071 928 742 399 272 263 181 85 BspEI T'CCGG_A Cuts at: 2406 5778 2406 Size: 3372 2743 BspHI T'CATG_A Cuts at: 4004 5734 4004 Size: 1730 4385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3470 844 Size: 2626 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4002 1394 Size: 429 2179 3507 Fragments arranged by size: 3507 2179 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4610 4784 65 Size: 1183 3362 174 1396 Fragments arranged by size: 3362 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4759 4946 1700 Size: 110 2949 187 2869 Fragments arranged by size: 2949 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6115 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6115 BssSI C'ACGA_G Cuts at: 2657 4175 5187 2657 Size: 1518 1012 3585 Fragments arranged by size: 3585 1518 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6115 BstBI TT'CG_AA Cuts at: 4919 4919 Size: 6115 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6115 BstXI CCAn_nnnn'nTGG Cuts at: 2558 2558 Size: 6115 BstYI r'GATC_y Cuts at: 1972 2567 2895 3338 3445 3812 4197 4214 5666 Size: 595 328 443 107 367 385 17 1452 Cuts at: 5666 5752 5764 6110 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 595 443 385 367 346 328 107 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2674 2674 Size: 6115 BtgI C'CryG_G Cuts at: 513 1065 2497 2883 513 Size: 552 1432 386 3745 Fragments arranged by size: 3745 1432 552 386 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5376 Fragments arranged by size: 5376 683 56 BtsI GCAGTG_nn' Cuts at: 2037 2646 3631 4425 4445 2037 Size: 609 985 794 20 3707 Fragments arranged by size: 3707 985 794 609 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6080 DraI TTT'AAA Cuts at: 3046 3419 3046 Size: 373 5742 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5122 809 Size: 507 3806 1802 Fragments arranged by size: 3806 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2665 2683 2978 Size: 54 1425 50 275 18 835 18 295 Cuts at: 2978 3562 4451 6080 8 Size: 584 889 1629 43 Fragments arranged by size: 1629 1425 889 835 584 295 275 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3562 1537 Size: 275 1750 4090 Fragments arranged by size: 4090 1750 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 4045 1353 Size: 705 1987 3423 Fragments arranged by size: 3423 1987 705 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4702 5076 5222 387 Size: 1064 138 3113 374 146 1280 Fragments arranged by size: 3113 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2051 2504 2051 Size: 453 5662 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2067 2276 2948 4178 4775 5562 5830 Size: 393 435 209 672 1230 597 787 268 Cuts at: 5830 1239 Size: 1524 Fragments arranged by size: 1524 1230 787 672 597 435 393 268 209 EcoO109I rG'GnC_Cy Cuts at: 2321 3177 2321 Size: 856 5259 EcoRI G'AATT_C Cuts at: 2606 3424 3802 2606 Size: 818 378 4919 Fragments arranged by size: 4919 818 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3056 3056 Size: 6115 FspI TGC'GCA Cuts at: 4621 4621 Size: 6115 HaeII r_GCGC'y Cuts at: 1678 5262 1678 Size: 3584 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2268 2300 4756 4788 4830 Size: 32 135 32 696 32 2456 32 42 Cuts at: 4830 4862 1373 Size: 32 2626 Fragments arranged by size: 2626 2456 696 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2395 2734 3459 3705 669 Size: 988 281 457 339 725 246 3079 Fragments arranged by size: 3079 988 725 457 339 281 246 HindIII A'AGCT_T Cuts at: 748 2412 748 Size: 1664 4451 HpaI GTT'AAC Cuts at: 3705 3705 Size: 6115 KpnI G_GTAC'C Cuts at: 3440 3440 Size: 6115 MfeI C'AATT_G Cuts at: 3714 3714 Size: 6115 MluI A'CGCG_T Cuts at: 5991 5991 Size: 6115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3394 5229 5413 1305 Size: 2089 1835 184 2007 Fragments arranged by size: 2089 2007 1835 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2667 2685 2980 10 Size: 54 1425 343 835 18 295 3145 Fragments arranged by size: 3145 1425 835 343 295 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2275 2468 2483 2595 2691 2882 4073 Size: 648 1109 193 15 112 96 191 1191 Cuts at: 4073 4432 4591 518 Size: 359 159 2042 Fragments arranged by size: 2042 1191 1109 648 359 193 191 159 112 96 15 MspA1I CmG'CkG Cuts at: 1606 3152 3531 4208 5356 5601 1606 Size: 1546 379 677 1148 245 2120 Fragments arranged by size: 2120 1546 1148 677 379 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5563 NdeI CA'TA_TG Cuts at: 387 387 Size: 6115 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6115 NotI GC'GGCC_GC Cuts at: 3562 3562 Size: 6115 NspI r_CATG'y Cuts at: 1132 1229 3473 5018 1132 Size: 97 2244 1545 2229 Fragments arranged by size: 2244 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6115 PciI A'CATG_T Cuts at: 5014 5014 Size: 6115 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6115 PmeI GTTT'AAAC Cuts at: 3419 3419 Size: 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2829 2861 4184 4216 5697 5729 2829 Size: 32 1323 32 1481 32 3215 Fragments arranged by size: 3215 1481 1323 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3177 3177 Size: 6115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6115 PsiI TTA'TAA Cuts at: 3685 3685 Size: 6115 PspOMI G'GGCC_C Cuts at: 2321 2321 Size: 6115 PstI C_TGCA'G Cuts at: 830 2034 3467 830 Size: 1204 1433 3478 Fragments arranged by size: 3478 1433 1204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6115 PvuII CAG'CTG Cuts at: 3152 3152 Size: 6115 SacI G_AGCT'C Cuts at: 2053 2506 2053 Size: 453 5662 SalI G'TCGA_C Cuts at: 1936 3457 1936 Size: 1521 4594 SapI GCTCTTCn'nnn_ Cuts at: 2058 2058 Size: 6115 SbfI CC_TGCA'GG Cuts at: 2034 3467 2034 Size: 1433 4682 ScaI AGT'ACT Cuts at: 4363 6075 4363 Size: 1712 4403 SfcI C'TryA_G Cuts at: 826 1045 2030 2350 3463 4598 5279 5470 826 Size: 219 985 320 1113 1135 681 191 1471 Fragments arranged by size: 1471 1135 1113 985 681 320 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6115 SmaI CCC'GGG Cuts at: 1872 3442 1872 Size: 1570 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 4219 5120 5382 820 Size: 197 926 189 2087 901 262 1553 Fragments arranged by size: 2087 1553 926 901 262 197 189 SnaBI TAC'GTA Cuts at: 493 3906 493 Size: 3413 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6115 SphI G_CATG'C Cuts at: 1132 3473 1132 Size: 2341 3774 SspI AAT'ATT Cuts at: 5 52 4039 5 Size: 47 3987 2081 Fragments arranged by size: 3987 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6115 StyI C'CwwG_G Cuts at: 513 1065 3271 3334 3554 513 Size: 552 2206 63 220 3074 Fragments arranged by size: 3074 2206 552 220 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4142 4159 4312 4497 4942 325 Size: 1255 2562 17 153 185 445 1498 Fragments arranged by size: 2562 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4361 6073 96 Size: 274 80 33 51 1443 2384 1712 138 Fragments arranged by size: 2384 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6075 6107 6075 Size: 32 6083 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6115 XbaI T'CTAG_A Cuts at: 3451 3451 Size: 6115 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2525 2525 Size: 6115 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6115 XmaI C'CCGG_G Cuts at: 1870 3440 1870 Size: 1570 4545 XmnI GAAnn'nnTTC Cuts at: 2414 4244 2414 Size: 1830 4285 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3923 276 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI