Restriction map for
pFN21AE1688
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsiEI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstNI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoRV
EcoT14I FauI FbaI FseI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI TaqI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AfaI 112 GTAC GT^AC
AflIII 539 ACATGT A^C[GA][CT]GT
AluI 413 AGCT AG^CT
Alw44I 517 GTGCAC G^TGCAC
AlwI 343 GATCC GGATC(4/5)
AlwNI 160 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 517 GTGCAC G^TGCAC
AspHI 517 GTGCAC G[AT]GC[AT]^C
AspI 136 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 297 GGACC G^G[AT]CC
AviII 38 TGCGCA TGC^GCA
BbvI 442 GCAGC GCAGC(8/12)
BfaI 575 CTAG C^TAG
BmyI 517 GTGCAC G[AGT]GC[ACT]^C
BseRI 347 CTCCTC GAGGAG(10/8)
BsgI 440 GTGCAG GTGCAG(16/14)
BsiHKAI 517 GTGCAC G[AT]GC[AT]^C
BsmAI 274 GTCTC GTCTC(1/5)
Bsp1286I 517 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 111 TGTACA T^GTACA
BspHI 545 TCATGA T^CATGA
BspLU11I 539 ACATGT A^CATGT
BsrGI 111 TGTACA T^GTACA
BsrI 512 CCAGT ACTGG(1/-1)
BstEII 425 GGTTACC G^GT[ACGT]ACC
BstPI 425 GGTTACC G^GT[ACGT]ACC
CfoI 39 GCGC GCG^C
Cfr13I 297 GGACC G^G[ACGT]CC
EarI 28 CTCTTC CTCTTC(1/4)
EcoO65I 425 GGTTACC G^GT[ACGT]ACC
EcoT22I 471 ATGCAT ATGCA^T
Fnu4HI 442 GCAGC GC^[ACGT]GC
FspI 38 TGCGCA TGC^GCA
HaeIII 10 GGCC GG^CC
HhaI 39 GCGC GCG^C
HinP1I 39 GCGC G^CGC
HphI 532 GGTGA GGTGA(8/7)
ItaI 442 GCAGC GC^[ACGT]GC
Ksp632I 28 CTCTTC CTCTTC(1/4)
MaeI 575 CTAG C^TAG
MboII 29 TCTTC GAAGA(8/7)
MspA1I 412 CAGCTG C[AC]G^C[GT]G
NsiI 471 ATGCAT ATGCA^T
NspI 539 ACATGT [GA]CATG^[CT]
PflFI 136 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 412 CAGCTG CAG^CTG
RcaI 545 TCATGA T^CATGA
RsaI 112 GTAC GT^AC
Sau96I 297 GGACC G^G[ACGT]CC
SfaNI 470 GATGC GCATC(5/9)
SspBI 111 TGTACA T^GTACA
StuI 9 AGGCCT AGG^CCT
TfiI 394 GAATC G^A[AT]TC
TseI 442 GCAGC G^C[AT]GC
Tsp45I 589 GTGAC ^GT[GC]AC
Tth111I 136 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 574 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 10 enzymes) #
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AcsI 116 AAATTT [GA]^AATT[CT]
241 AAATTC
ApoI 116 AAATTT [GA]^AATT[CT]
241 AAATTC
Cac8I 35 GCTTGC GC[ACGT]^[ACGT]GC
410 GCCAGC
DdeI 197 CTAAG C^T[ACGT]AG
254 CTCAG
DpnII 190 GATC ^GATC
343 GATC
DraI 151 TTTAAA TTT^AAA
355 TTTAAA
MboI 190 GATC ^GATC
343 GATC
NdeII 190 GATC ^GATC
343 GATC
PleI 578 GACTC GAGTC(4/5)
234 GAGTC
Sau3AI 190 GATC ^GATC
343 GATC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BstF5I FokI HinfI MaeIII MnlI
MseI MslI NlaIII Tru9I Tsp509I
TspRI