################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BclI BfrI BlnI BpuAI BsaAI BsaBI BsaHI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI HaeII Hin1I HincII HindII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Alw44I 1183 GTGCAC G^TGCAC ApaLI 1183 GTGCAC G^TGCAC Asp700I 492 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 749 TGCGCA TGC^GCA BalI 27 TGGCCA TGG^CCA BcgI 38 GCAACCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1255 GTATCC GTATCC(6/5) BglII 1127 AGATCT A^GATCT BlpI 1080 GCTGAGC GC^T[ACGT]AGC BpmI 130 CTGGAG CTGGAG(16/14) Bpu1102I 1080 GCTGAGC GC^T[ACGT]AGC BsaI 1140 GGTCTC GGTCTC(1/5) BsiEI 105 CGACCG CG[GA][CT]^CG BsmAI 1141 GTCTC GTCTC(1/5) BspMI 746 ACCTGC ACCTGC(4/8) BsrBI 699 GAGCGG CCGCTC(-3/-3) BssSI 364 CACGAG CACGAG(-5/-1) BstXI 968 CCAAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1080 GCTGAGC GC^T[ACGT]AGC CfoI 750 GCGC GCG^C CpoI 479 CGGACCG CG^G[AT]CCG DraIII 679 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 262 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1306 GAAGAG CTCTTC(1/4) EcoNI 161 CCTACGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 492 GAATTC G^AATTC FspI 749 TGCGCA TGC^GCA HgaI 503 GCGTC GACGC(5/10) HhaI 750 GCGC GCG^C HinP1I 750 GCGC G^CGC HindIII 1175 AAGCTT A^AGCTT Ksp632I 1306 GAAGAG CTCTTC(1/4) KspI 108 CCGCGG CCGC^GG MaeII 1043 ACGT A^CGT MluNI 27 TGGCCA TGG^CCA MscI 27 TGGCCA TGG^CCA MseI 1133 TTAA T^TAA PstI 388 CTGCAG CTGCA^G RsrII 479 CGGACCG CG^G[AT]CCG SacII 108 CCGCGG CCGC^GG SbfI 387 CCTGCAGG CCTGCA^GG SfcI 388 CTGCAG C^T[GA][CT]AG Sse8387I 387 CCTGCAGG CCTGCA^GG Tru9I 1133 TTAA T^TAA XbaI 1293 TCTAGA T^CTAGA XmnI 492 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 109 CGCG CG^CG 552 CGCG AcsI 492 GAATTC [GA]^AATT[CT] 1219 GAATTT AlwNI 982 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG 1011 CAGCAGCTG ApoI 492 GAATTC [GA]^AATT[CT] 1219 GAATTT BsaWI 482 ACCGGA [AT]^CCGG[AT] 719 TCCGGT BseRI 469 GAGGAG GAGGAG(10/8) 1318 GAGGAG BsgI 787 CTGCAC GTGCAG(16/14) 997 GTGCAG BsmI 582 GAATGC GAATGC(1/-1) 224 GCATTC BspHI 452 TCATGA T^CATGA 488 TCATGA BstUI 109 CGCG CG^CG 552 CGCG BstYI 669 GGATCT [GA]^GATC[CT] 1127 AGATCT DraII 112 GGGGCCT [GA]G^G[ACGT]CC[CT] 614 AGGCCCT EcoO109I 112 GGGGCCT [GA]G^G[ACGT]CC[CT] 614 AGGCCCT HinfI 137 GAATC G^A[ACGT]TC 1032 GATTC MflI 669 GGATCT [GA]^GATC[CT] 1127 AGATCT MslI 364 CACGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 531 CACGGTGGTG MvnI 109 CGCG CG^CG 552 CGCG NcoI 273 CCATGG C^CATGG 896 CCATGG RcaI 452 TCATGA T^CATGA 488 TCATGA SacI 245 GAGCTC GAGCT^C 862 GAGCTC SmaI 726 CCCGGG CCC^GGG 1073 CCCGGG TfiI 137 GAATC G^A[AT]TC 1032 GATTC XhoII 669 GGATCT [GA]^GATC[CT] 1127 AGATCT XmaCI 726 CCCGGG C^CCGGG 1073 CCCGGG XmaI 726 CCCGGG C^CCGGG 1073 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI