(Circular) (Six-base) MAPSORT of: pFN21AE1914.seq Check: 928 from: 1 to: 6058 pFN21AE1914 6058 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3401 1937 Size: 1464 4594 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2488 2669 4121 5505 5773 1632 Size: 856 181 1452 1384 268 1917 Fragments arranged by size: 1917 1452 1384 856 268 181 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 3355 4957 5934 1203 Size: 2152 1602 977 1327 Fragments arranged by size: 2152 1602 1327 977 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 4787 4787 Size: 6058 AleI CACnn'nnGTG Cuts at: 1166 2373 2540 1166 Size: 1207 167 4684 Fragments arranged by size: 4684 1207 167 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6058 AlwNI CAG_nnn'CTG Cuts at: 2992 3021 3801 5373 2992 Size: 29 780 1572 3677 Fragments arranged by size: 3677 1572 780 29 ApaLI G'TGCA_C Cuts at: 3188 4115 5271 3188 Size: 927 1156 3975 Fragments arranged by size: 3975 1156 927 ApoI r'AATT_y Cuts at: 1493 2497 3224 3367 3593 3745 5854 1493 Size: 1004 727 143 226 152 2109 1697 Fragments arranged by size: 2109 1697 1004 727 226 152 143 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 2187 2731 3078 3383 1870 Size: 73 244 544 347 305 4545 Fragments arranged by size: 4545 544 347 305 244 73 BamHI G'GATC_C Cuts at: 3388 3755 3388 Size: 367 5691 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3379 4834 618 Size: 325 217 627 1592 1455 1842 Fragments arranged by size: 1842 1592 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2254 2550 2854 2871 3377 1391 Size: 863 296 304 17 506 4072 Fragments arranged by size: 4072 863 506 304 296 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5087 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2032 2066 4249 4283 1903 Size: 34 50 34 11 34 2183 34 3678 Fragments arranged by size: 3678 2183 50 34 34 34 34 11 BciVI GTATCCnnnnn_n' Cuts at: 3271 3950 5160 3271 Size: 679 1210 4169 Fragments arranged by size: 4169 1210 679 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 2397 3082 Size: 107 122 71 631 605 368 357 685 Cuts at: 3082 4669 136 Size: 1587 1525 Fragments arranged by size: 1587 1525 685 631 605 368 357 122 107 71 BglII A'GATC_T Cuts at: 3132 6053 3132 Size: 2921 3137 BlpI GC'TnA_GC Cuts at: 3086 3475 3086 Size: 389 5669 Bme1580I G_kGCm'C Cuts at: 1790 2187 2432 2450 3192 4119 5275 1790 Size: 397 245 18 742 927 1156 2573 Fragments arranged by size: 2573 1156 927 742 397 245 18 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 3789 4747 Size: 416 53 83 399 24 897 1475 958 Cuts at: 4747 442 Size: 1753 Fragments arranged by size: 1753 1475 958 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6058 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3175 3175 Size: 6058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 4718 1239 Size: 917 2562 2579 Fragments arranged by size: 2579 2562 917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4183 5048 5346 4183 Size: 865 298 4895 Fragments arranged by size: 4895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3151 4721 882 Size: 617 1652 1570 2219 Fragments arranged by size: 2219 1652 1570 617 BsaAI yAC'GTr Cuts at: 493 1204 3849 493 Size: 711 2645 2702 Fragments arranged by size: 2702 2645 711 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3865 4247 4882 275 Size: 53 83 186 936 2332 382 635 1451 Fragments arranged by size: 2332 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2487 2724 4491 4889 5163 5310 5721 2487 Size: 237 1767 398 274 147 411 2824 Fragments arranged by size: 2824 1767 411 398 274 237 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5833 Fragments arranged by size: 5833 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2489 3338 1186 Size: 1303 849 3906 Fragments arranged by size: 3906 1303 849 BseYI C'CCAG_C Cuts at: 2102 2874 5261 5757 2102 Size: 772 2387 496 2403 Fragments arranged by size: 2403 2387 772 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2777 3023 1163 Size: 573 1041 246 4198 Fragments arranged by size: 4198 1041 573 246 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2113 3508 4269 5297 1540 Size: 275 186 112 1395 761 1028 2301 Fragments arranged by size: 2301 1395 1028 761 275 186 112 BsiHKAI G_wGCw'C Cuts at: 2254 2871 3192 4119 4204 5275 2254 Size: 617 321 927 85 1071 3037 Fragments arranged by size: 3037 1071 927 617 321 85 BsmI GAATG_Cn' Cuts at: 2229 2593 3569 3662 2229 Size: 364 976 93 4625 Fragments arranged by size: 4625 976 364 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2254 2432 2450 2550 2854 2871 Size: 399 397 67 178 18 100 304 17 Cuts at: 2871 3192 3377 4119 4204 5275 1391 Size: 321 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 397 321 304 185 178 100 85 67 18 17 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 2457 2493 3947 5677 2457 Size: 36 1454 1730 2838 Fragments arranged by size: 2838 1730 1454 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2760 3413 844 Size: 1916 653 3489 Fragments arranged by size: 3489 1916 653 BsrBI CCG'CTC Cuts at: 1394 1823 2706 3945 1394 Size: 429 883 1239 3507 Fragments arranged by size: 3507 1239 883 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4553 4727 65 Size: 1183 3305 174 1396 Fragments arranged by size: 3305 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4702 4889 1700 Size: 110 2892 187 2869 Fragments arranged by size: 2892 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6058 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 2369 4118 5130 2369 Size: 1749 1012 3297 Fragments arranged by size: 3297 1749 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6058 BstBI TT'CG_AA Cuts at: 4862 4862 Size: 6058 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstXI CCAn_nnnn'nTGG Cuts at: 2980 2980 Size: 6058 BstYI r'GATC_y Cuts at: 1972 2674 3132 3388 3755 4140 4157 5609 5695 Size: 702 458 256 367 385 17 1452 86 Cuts at: 5695 5707 6053 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 702 458 385 367 346 256 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2113 2278 2859 2901 2923 513 Size: 552 1048 165 581 42 22 3648 Fragments arranged by size: 3648 1048 581 552 165 42 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5319 Fragments arranged by size: 5319 683 56 BtsI GCAGTG_nn' Cuts at: 3574 4368 4388 3574 Size: 794 20 5244 Fragments arranged by size: 5244 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3088 3123 529 Size: 35 2524 35 3464 Fragments arranged by size: 3464 2524 35 35 DraI TTT'AAA Cuts at: 3362 3362 Size: 6058 DraIII CAC_nnn'GTG Cuts at: 2689 2689 Size: 6058 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2273 5065 809 Size: 507 957 2792 1802 Fragments arranged by size: 2792 1802 957 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2862 2926 Size: 54 1425 50 275 18 202 830 64 Cuts at: 2926 3093 3120 3505 4394 6023 8 Size: 167 27 385 889 1629 43 Fragments arranged by size: 1629 1425 889 830 385 275 202 167 64 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3505 1537 Size: 275 1693 4090 Fragments arranged by size: 4090 1693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3306 3988 1353 Size: 1953 682 3423 Fragments arranged by size: 3423 1953 682 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2909 4645 5019 5165 387 Size: 1064 138 1320 1736 374 146 1280 Fragments arranged by size: 1736 1320 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2252 2869 2252 Size: 617 5441 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2488 2669 4121 4718 5505 5773 Size: 393 524 332 181 1452 597 787 268 Cuts at: 5773 1239 Size: 1524 Fragments arranged by size: 1524 1452 787 597 524 393 332 268 181 EcoNI CCTnn'n_nnAGG Cuts at: 2170 2170 Size: 6058 EcoO109I rG'GnC_Cy Cuts at: 2118 2620 2118 Size: 502 5556 EcoRI G'AATT_C Cuts at: 2497 3367 3745 2497 Size: 870 378 4810 Fragments arranged by size: 4810 870 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3198 3198 Size: 6058 FspI TGC'GCA Cuts at: 2756 4564 2756 Size: 1808 4250 HaeII r_GCGC'y Cuts at: 1678 5205 1678 Size: 3527 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2434 2466 3245 3277 4699 Size: 32 135 32 862 32 779 32 1422 Cuts at: 4699 4731 4773 4805 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1422 862 779 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3402 3648 669 Size: 988 281 1464 246 3079 Fragments arranged by size: 3079 1464 988 281 246 HindIII A'AGCT_T Cuts at: 748 3180 748 Size: 2432 3626 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 3657 3657 Size: 6058 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2511 5172 5356 1305 Size: 1206 2661 184 2007 Fragments arranged by size: 2661 2007 1206 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2034 10 Size: 54 1425 343 202 4034 Fragments arranged by size: 4034 1425 343 202 54 MslI CAynn'nnrTG Cuts at: 518 1166 2373 2540 4016 4375 4534 518 Size: 648 1207 167 1476 359 159 2042 Fragments arranged by size: 2042 1476 1207 648 359 167 159 MspA1I CmG'CkG Cuts at: 1606 2115 2134 2578 3021 3474 4151 5299 5544 Size: 509 19 444 443 453 677 1148 245 Cuts at: 5544 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 509 453 444 443 245 19 NcoI C'CATG_G Cuts at: 513 1065 2278 2901 513 Size: 552 1213 623 3670 Fragments arranged by size: 3670 1213 623 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6058 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6058 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NspI r_CATG'y Cuts at: 1132 1229 3359 3416 4961 1132 Size: 97 2130 57 1545 2229 Fragments arranged by size: 2229 2130 1545 97 57 PasI CC'CwG_GG Cuts at: 1348 2419 2830 1348 Size: 1071 411 4576 Fragments arranged by size: 4576 1071 411 PciI A'CATG_T Cuts at: 3355 4957 3355 Size: 1602 4456 PflMI CCAn_nnn'nTGG Cuts at: 1108 2064 2419 3256 1108 Size: 956 355 837 3910 Fragments arranged by size: 3910 956 837 355 PfoI T'CCnGG_A Cuts at: 2438 2438 Size: 6058 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6058 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PstI C_TGCA'G Cuts at: 830 2397 3410 830 Size: 1567 1013 3478 Fragments arranged by size: 3478 1567 1013 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 PvuII CAG'CTG Cuts at: 2134 2578 3021 2134 Size: 444 443 5171 Fragments arranged by size: 5171 444 443 RsrII CG'GwC_CG Cuts at: 2485 2485 Size: 6058 SacI G_AGCT'C Cuts at: 2254 2871 2254 Size: 617 5441 SacII CC_GC'GG Cuts at: 2116 2116 Size: 6058 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SbfI CC_TGCA'GG Cuts at: 2397 3410 2397 Size: 1013 5045 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SfcI C'TryA_G Cuts at: 826 1045 2393 3406 4541 5222 5413 826 Size: 219 1348 1013 1135 681 191 1471 Fragments arranged by size: 1471 1348 1135 1013 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 2733 3080 3385 1872 Size: 861 347 305 4545 Fragments arranged by size: 4545 861 347 305 SmlI C'TyrA_G Cuts at: 820 1017 1943 4162 5063 5325 820 Size: 197 926 2219 901 262 1553 Fragments arranged by size: 2219 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3416 1132 Size: 2284 3774 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6058 StyI C'CwwG_G Cuts at: 513 1065 2278 2523 2901 2973 3497 513 Size: 552 1213 245 378 72 524 3074 Fragments arranged by size: 3074 1213 552 524 378 245 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2677 2888 4085 4102 4255 4440 4885 Size: 1255 1097 211 1197 17 153 185 445 Cuts at: 4885 325 Size: 1498 Fragments arranged by size: 1498 1255 1197 1097 445 211 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 4304 6016 Size: 274 80 33 51 1443 174 2153 1712 Cuts at: 6016 96 Size: 138 Fragments arranged by size: 2153 1712 1443 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2675 2707 6018 6050 2675 Size: 32 3311 32 2683 Fragments arranged by size: 3311 2683 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 3298 3394 3298 Size: 96 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2027 2285 2977 3208 2027 Size: 258 692 231 4877 Fragments arranged by size: 4877 692 258 231 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6058 XmaI C'CCGG_G Cuts at: 1870 2731 3078 3383 1870 Size: 861 347 305 4545 Fragments arranged by size: 4545 861 347 305 XmnI GAAnn'nnTTC Cuts at: 2501 4187 2501 Size: 1686 4372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI SanDI SapI SexAI SfiI SfoI SrfI SwaI