(Circular) (Six-base) MAPSORT of: pFN21AE1916.seq Check: 1073 from: 1 to: 6064 pFN21AE1916 6064 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2360 2751 3407 1937 Size: 423 391 656 4594 Fragments arranged by size: 4594 656 423 391 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 3363 4191 4564 1563 Size: 1800 828 373 3063 Fragments arranged by size: 3063 1800 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2859 3103 4127 5511 5779 1632 Size: 1227 244 1024 1384 268 1917 Fragments arranged by size: 1917 1384 1227 1024 268 244 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 2742 4963 5940 1203 Size: 1539 2221 977 1327 Fragments arranged by size: 2221 1539 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 2938 4793 2938 Size: 1855 4209 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 3807 5379 3807 Size: 1572 4492 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 2532 2563 2897 3373 3599 3751 5860 1493 Size: 1039 31 334 476 226 152 2109 1697 Fragments arranged by size: 2109 1697 1039 476 334 226 152 31 AseI AT'TA_AT Cuts at: 160 1032 2310 2622 4618 160 Size: 872 1278 312 1996 1606 Fragments arranged by size: 1996 1606 1278 872 312 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 3166 3389 1870 Size: 73 1223 223 4545 Fragments arranged by size: 4545 1223 223 73 BamHI G'GATC_C Cuts at: 3394 3761 3394 Size: 367 5697 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3294 3385 4840 618 Size: 325 217 627 1507 91 1455 1842 Fragments arranged by size: 1842 1507 1455 627 325 217 91 BanII G_rGCy'C Cuts at: 1391 3383 1391 Size: 1992 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2452 928 Size: 971 553 4540 Fragments arranged by size: 4540 971 553 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2728 2762 4255 4289 1903 Size: 34 791 34 1493 34 3678 Fragments arranged by size: 3678 1493 791 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3956 5166 3956 Size: 1210 4854 BclI T'GATC_A Cuts at: 1548 2584 1548 Size: 1036 5028 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 4675 136 Size: 107 122 71 631 605 365 2638 1525 Fragments arranged by size: 2638 1525 631 605 365 122 107 71 BglII A'GATC_T Cuts at: 2143 6059 2143 Size: 3916 2148 BlpI GC'TnA_GC Cuts at: 3481 3481 Size: 6064 Bme1580I G_kGCm'C Cuts at: 1790 4125 5281 1790 Size: 2335 1156 2573 Fragments arranged by size: 2573 2335 1156 BmgBI CAC'GTC Cuts at: 1318 2095 1318 Size: 777 5287 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3795 4753 442 Size: 416 53 83 399 24 2378 958 1753 Fragments arranged by size: 2378 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2160 3179 4724 1239 Size: 921 1019 1545 2579 Fragments arranged by size: 2579 1545 1019 921 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4189 5054 5352 4189 Size: 865 298 4901 Fragments arranged by size: 4901 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2448 2978 4727 882 Size: 617 949 530 1749 2219 Fragments arranged by size: 2219 1749 949 617 530 BsaAI yAC'GTr Cuts at: 493 1204 3855 493 Size: 711 2651 2702 Fragments arranged by size: 2702 2651 711 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3871 4253 4888 275 Size: 53 83 186 936 2338 382 635 1451 Fragments arranged by size: 2338 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2266 4497 4895 5169 5316 5727 2266 Size: 2231 398 274 147 411 2603 Fragments arranged by size: 2603 2231 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2777 2801 2807 2831 1178 Size: 30 165 30 1374 24 6 24 4411 Fragments arranged by size: 4411 1374 165 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2705 2785 1186 Size: 1519 80 4465 Fragments arranged by size: 4465 1519 80 BseYI C'CCAG_C Cuts at: 2846 5267 5763 2846 Size: 2421 496 3147 Fragments arranged by size: 3147 2421 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3266 1163 Size: 573 1530 3961 Fragments arranged by size: 3961 1530 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4125 4210 5281 4125 Size: 85 1071 4908 Fragments arranged by size: 4908 1071 85 BsmI GAATG_Cn' Cuts at: 2344 3575 3668 2344 Size: 1231 93 4740 Fragments arranged by size: 4740 1231 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3383 4125 4210 5281 1391 Size: 399 1593 742 85 1071 2174 Fragments arranged by size: 2174 1593 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 2260 2587 3093 3953 5683 2260 Size: 327 506 860 1730 2641 Fragments arranged by size: 2641 1730 860 506 327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2059 3015 3419 844 Size: 1215 956 404 3489 Fragments arranged by size: 3489 1215 956 404 BsrBI CCG'CTC Cuts at: 1394 1823 3951 1394 Size: 429 2128 3507 Fragments arranged by size: 3507 2128 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3312 4559 4733 65 Size: 1183 2064 1247 174 1396 Fragments arranged by size: 2064 1396 1247 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2977 4708 4895 1700 Size: 110 1167 1731 187 2869 Fragments arranged by size: 2869 1731 1167 187 110 BsrGI T'GTAC_A Cuts at: 96 3339 96 Size: 3243 2821 BssHII G'CGCG_C Cuts at: 1925 3187 1925 Size: 1262 4802 BssSI C'ACGA_G Cuts at: 2999 4124 5136 2999 Size: 1125 1012 3927 Fragments arranged by size: 3927 1125 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3021 794 Size: 2227 3837 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 2325 1184 Size: 1141 4923 BstYI r'GATC_y Cuts at: 1972 2143 2503 2842 3276 3394 3761 4146 4163 Size: 171 360 339 434 118 367 385 17 Cuts at: 4163 5615 5701 5713 6059 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 434 385 367 360 346 339 171 118 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 2499 2525 3300 3318 513 Size: 552 939 30 465 26 775 18 3259 Fragments arranged by size: 3259 939 775 552 465 30 26 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5325 Fragments arranged by size: 5325 683 56 BtsI GCAGTG_nn' Cuts at: 2709 3580 4374 4394 2709 Size: 871 794 20 4379 Fragments arranged by size: 4379 871 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DraIII CAC_nnn'GTG Cuts at: 3301 3301 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 3511 4400 Size: 54 1425 50 275 18 267 1414 889 Cuts at: 4400 6029 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1414 889 275 267 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2826 3994 1353 Size: 1473 1168 3423 Fragments arranged by size: 3423 1473 1168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2765 4651 5025 5171 387 Size: 1064 138 1176 1886 374 146 1280 Fragments arranged by size: 1886 1280 1176 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2160 2859 3103 3179 4127 4724 5511 Size: 393 528 699 244 76 948 597 787 Cuts at: 5511 5779 1239 Size: 268 1524 Fragments arranged by size: 1524 948 787 699 597 528 393 268 244 76 EcoO109I rG'GnC_Cy Cuts at: 2482 2856 2956 3102 2482 Size: 374 100 146 5444 Fragments arranged by size: 5444 374 146 100 EcoRI G'AATT_C Cuts at: 3373 3751 3373 Size: 378 5686 FspI TGC'GCA Cuts at: 3011 4570 3011 Size: 1559 4505 HaeII r_GCGC'y Cuts at: 1678 2477 3090 5211 1678 Size: 799 613 2121 2531 Fragments arranged by size: 2531 2121 799 613 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2777 2801 2809 2833 2924 Size: 32 135 32 1205 24 8 24 91 Cuts at: 2924 2956 4705 4737 4779 4811 1373 Size: 32 1749 32 42 32 2626 Fragments arranged by size: 2626 1749 1205 135 91 42 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 3408 3654 669 Size: 988 281 1470 246 3079 Fragments arranged by size: 3079 1470 988 281 246 HindIII A'AGCT_T Cuts at: 748 2653 748 Size: 1905 4159 HpaI GTT'AAC Cuts at: 3654 3654 Size: 6064 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5178 5362 1305 Size: 3873 184 2007 Fragments arranged by size: 3873 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4242 Fragments arranged by size: 4242 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3317 4022 4381 4540 518 Size: 648 2151 705 359 159 2042 Fragments arranged by size: 2151 2042 705 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2036 3480 4157 5305 5550 1606 Size: 430 1444 677 1148 245 2120 Fragments arranged by size: 2120 1444 1148 677 430 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2499 513 Size: 552 939 495 4078 Fragments arranged by size: 4078 939 552 495 NdeI CA'TA_TG Cuts at: 387 387 Size: 6064 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2791 3422 4967 1132 Size: 97 1562 631 1545 2229 Fragments arranged by size: 2229 1562 1545 631 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 4963 4963 Size: 6064 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6064 PfoI T'CCnGG_A Cuts at: 3348 3348 Size: 6064 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2482 2856 2956 3102 2482 Size: 374 100 146 5444 Fragments arranged by size: 5444 374 146 100 PshAI GACnn'nnGTC Cuts at: 1613 2702 1613 Size: 1089 4975 PsiI TTA'TAA Cuts at: 2424 3634 2424 Size: 1210 4854 PstI C_TGCA'G Cuts at: 830 2608 2716 3416 830 Size: 1778 108 700 3478 Fragments arranged by size: 3478 1778 700 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 SacII CC_GC'GG Cuts at: 2037 2037 Size: 6064 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SanDI GG'GwC_CC Cuts at: 2482 2482 Size: 6064 SapI GCTCTTCn'nnn_ Cuts at: 2826 2826 Size: 6064 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SexAI A'CCwGG_T Cuts at: 2991 2991 Size: 6064 SfcI C'TryA_G Cuts at: 826 1045 2604 2712 2752 3412 4547 5228 5419 Size: 219 1559 108 40 660 1135 681 191 Cuts at: 5419 826 Size: 1471 Fragments arranged by size: 1559 1471 1135 681 660 219 191 108 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3166 4168 5069 5331 820 Size: 197 926 1223 1002 901 262 1553 Fragments arranged by size: 1553 1223 1002 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 3422 1132 Size: 2290 3774 SspI AAT'ATT Cuts at: 5 52 2490 3988 5 Size: 47 2438 1498 2081 Fragments arranged by size: 2438 2081 1498 47 StuI AGG'CCT Cuts at: 1517 3164 1517 Size: 1647 4417 StyI C'CwwG_G Cuts at: 513 1065 2004 2499 3503 513 Size: 552 939 495 1004 3074 Fragments arranged by size: 3074 1004 939 552 495 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2714 4091 4108 4261 4446 4891 325 Size: 1255 1134 1377 17 153 185 445 1498 Fragments arranged by size: 1498 1377 1255 1134 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3339 4310 6022 Size: 274 80 33 51 1443 1362 971 1712 Cuts at: 6022 96 Size: 138 Fragments arranged by size: 1712 1443 1362 971 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2380 2412 6024 6056 2380 Size: 32 3612 32 2388 Fragments arranged by size: 3612 2388 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3100 3308 1316 Size: 1784 208 4072 Fragments arranged by size: 4072 1784 208 XbaI T'CTAG_A Cuts at: 2290 3400 2290 Size: 1110 4954 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2506 2506 Size: 6064 XhoI C'TCGA_G Cuts at: 1943 3166 1943 Size: 1223 4841 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 4193 4193 Size: 6064 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SfiI SfoI SrfI SwaI