################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII BbrPI BbsI BclI BfrI BglII BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI KspI MamI MfeI MluI MroI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 410 GTATAC GT^[AC][GT]AC AfaI 1133 GTAC GT^AC Aor51HI 808 AGCGCT AGC^GCT Asp700I 1476 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 1464 CCTAGG C^CTAGG BamHI 1375 GGATCC G^GATCC BbvCI 1071 GCTGAGG CCTCAGC(-5/-2) BciVI 673 GTATCC GTATCC(6/5) BlnI 1464 CCTAGG C^CTAGG BsaI 480 GGTCTC GGTCTC(1/5) BspLU11I 1129 ACATGT A^CATGT BsrBI 754 GAGCGG CCGCTC(-3/-3) BsrDI 791 CATTGC GCAATG(2/0) Bst1107I 410 GTATAC GTA^TAC BstEII 1105 GGTGACC G^GT[ACGT]ACC BstPI 1105 GGTGACC G^GT[ACGT]ACC BstXI 1293 CCAGACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 410 GTATAC GTA^TAC EarI 1391 CTCTTC CTCTTC(1/4) Eco47III 808 AGCGCT AGC^GCT EcoNI 415 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1105 GGTGACC G^GT[ACGT]ACC HgaI 610 GCGTC GACGC(5/10) HindIII 1041 AAGCTT A^AGCTT Ksp632I 1391 CTCTTC CTCTTC(1/4) MspA1I 1069 CAGCTG C[AC]G^C[GT]G NaeI 559 GCCGGC GCC^GGC NgoMIV 559 GCCGGC G^CCGGC PpuMI 706 GGGTCCC [GA]G^G[AT]CC[CT] PvuI 698 CGATCG CGAT^CG PvuII 1069 CAGCTG CAG^CTG RsaI 1133 GTAC GT^AC SacI 117 GAGCTC GAGCT^C SfaNI 291 GCATC GCATC(5/9) SfiI 570 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 423 AGGCCT AGG^CCT XmnI 1476 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 209 ACGTGT A^C[GA][CT]GT 1129 ACATGT BalI 267 TGGCCA TGG^CCA 1290 TGGCCA BcgI 925 CGACAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 692 GCACCGCGATCG BglI 487 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 571 GCCGCCTGGGC BlpI 804 GCTGAGC GC^T[ACGT]AGC 915 GCTAAGC Bpu1102I 804 GCTGAGC GC^T[ACGT]AGC 915 GCTAAGC BsgI 886 GTGCAG GTGCAG(16/14) 1003 GTGCAG BsiEI 614 CGGTCG CG[GA][CT]^CG 698 CGATCG BsmAI 1057 GAGAC GTCTC(1/5) 481 GTCTC BspMI 414 ACCTGC ACCTGC(4/8) 1224 GCAGGT BsrFI 559 GCCGGC [GA]^CCGG[CT] 1109 ACCGGC BstF5I 340 CATCC GGATG(2/0) 273 GGATG BstYI 536 GGATCT [GA]^GATC[CT] 1375 GGATCC CelII 804 GCTGAGC GC^T[ACGT]AGC 915 GCTAAGC Cfr10I 559 GCCGGC [GA]^CCGG[CT] 1109 ACCGGC FokI 340 CATCC GGATG(9/13) 273 GGATG MaeII 209 ACGT A^CGT 668 ACGT MboII 1392 TCTTC GAAGA(8/7) 1481 TCTTC MflI 536 GGATCT [GA]^GATC[CT] 1375 GGATCC MluNI 267 TGGCCA TGG^CCA 1290 TGGCCA MscI 267 TGGCCA TGG^CCA 1290 TGGCCA NspI 1129 ACATGT [GA]CATG^[CT] 1347 GCATGT PleI 56 GACTC GAGTC(4/5) 1447 GACTC PstI 158 CTGCAG CTGCA^G 862 CTGCAG SmlI 782 CTCAAG C^T[CT][GA]AG 1504 CTCAAG TfiI 1164 GAATC G^A[AT]TC 1384 GAATC Tsp45I 1106 GTGAC ^GT[GC]AC 1153 GTCAC XcmI 169 CCAAAAGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 255 CCAACATTGGGTTGG XhoII 536 GGATCT [GA]^GATC[CT] 1375 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeIII MboI MnlI MseI MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PflMI Sau3AI Sau96I ScrFI SfcI SfoI StyI Tru9I TseI TspRI Van91I