(Circular) (Six-base) MAPSORT of: pFN21AE1958.seq Check: 3199 from: 1 to: 6247 pFN21AE1958 6247 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3220 3220 Size: 6247 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4057 278 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2416 3590 1937 Size: 479 1174 4594 Fragments arranged by size: 4594 1174 479 Acc65I G'GTAC_C Cuts at: 3568 3568 Size: 6247 AclI AA'CG_TT Cuts at: 1563 4374 4747 1563 Size: 2811 373 3063 Fragments arranged by size: 3063 2811 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2612 2772 4310 5694 5962 1632 Size: 980 160 1538 1384 268 1917 Fragments arranged by size: 1917 1538 1384 980 268 160 AfeI AGC'GCT Cuts at: 2815 2815 Size: 6247 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6050 AflIII A'CryG_T Cuts at: 1203 2214 3134 5146 6123 1203 Size: 1011 920 2012 977 1327 Fragments arranged by size: 2012 1327 1011 977 920 AgeI A'CCGG_T Cuts at: 5078 5078 Size: 6247 AhdI GACnn_n'nnGTC Cuts at: 4976 4976 Size: 6247 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6247 AlwNI CAG_nnn'CTG Cuts at: 2029 2137 2162 2538 2875 3990 5562 2029 Size: 108 25 376 337 1115 1572 2714 Fragments arranged by size: 2714 1572 1115 376 337 108 25 ApaI G_GGCC'C Cuts at: 2036 2587 2826 2036 Size: 551 239 5457 Fragments arranged by size: 5457 551 239 ApaLI G'TGCA_C Cuts at: 4304 5460 4304 Size: 1156 5091 ApoI r'AATT_y Cuts at: 1493 3556 3782 3934 6043 1493 Size: 2063 226 152 2109 1697 Fragments arranged by size: 2109 2063 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4801 160 Size: 872 3769 1606 Fragments arranged by size: 3769 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 AvaI C'yCGr_G Cuts at: 1870 1943 2773 2951 3054 3572 1870 Size: 73 830 178 103 518 4545 Fragments arranged by size: 4545 830 518 178 103 73 AvrII C'CTAG_G Cuts at: 3469 3469 Size: 6247 BamHI G'GATC_C Cuts at: 3380 3577 3944 3380 Size: 197 367 5683 Fragments arranged by size: 5683 367 197 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2088 2372 3433 3474 3568 Size: 325 217 627 301 284 1061 41 94 Cuts at: 3568 5023 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1061 627 325 301 284 217 94 41 BanII G_rGCy'C Cuts at: 1391 2036 2126 2587 2826 3243 3566 1391 Size: 645 90 461 239 417 323 4072 Fragments arranged by size: 4072 645 461 417 323 239 90 BbeI G_GCGC'C Cuts at: 2092 2376 3478 2092 Size: 284 1102 4861 Fragments arranged by size: 4861 1102 284 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5276 BbvCI CC'TCA_GC Cuts at: 3077 3077 Size: 6247 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2686 2720 2919 2953 4438 4472 1903 Size: 34 749 34 199 34 1485 34 3678 Fragments arranged by size: 3678 1485 749 199 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2689 4139 5349 2689 Size: 1450 1210 3587 Fragments arranged by size: 3587 1450 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2498 2582 4858 Size: 107 122 71 631 605 826 84 2276 Cuts at: 4858 136 Size: 1525 Fragments arranged by size: 2276 1525 826 631 605 122 107 84 71 BglII A'GATC_T Cuts at: 6242 6242 Size: 6247 BlpI GC'TnA_GC Cuts at: 2810 2921 3664 2810 Size: 111 743 5393 Fragments arranged by size: 5393 743 111 Bme1580I G_kGCm'C Cuts at: 1790 2036 2587 2826 4308 5464 1790 Size: 246 551 239 1482 1156 2573 Fragments arranged by size: 2573 1482 1156 551 246 239 BmgBI CAC'GTC Cuts at: 1318 2674 1318 Size: 1356 4891 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2017 2257 3978 Size: 416 53 83 399 24 600 240 1721 Cuts at: 3978 4936 442 Size: 958 1753 Fragments arranged by size: 1753 1721 958 600 416 399 240 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6247 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3463 3463 Size: 6247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 2210 2445 2515 2738 4907 1239 Size: 816 155 235 70 223 2169 2579 Fragments arranged by size: 2579 2169 816 235 223 155 70 Bpu10I CC'TnA_GC Cuts at: 2144 3077 2144 Size: 933 5314 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2772 3494 4372 5237 5535 2772 Size: 722 878 865 298 3484 Fragments arranged by size: 3484 878 865 722 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2491 4910 882 Size: 617 992 2419 2219 Fragments arranged by size: 2419 2219 992 617 BsaAI yAC'GTr Cuts at: 493 1204 4038 493 Size: 711 2834 2702 Fragments arranged by size: 2834 2702 711 BsaBI GATnn'nnATC Cuts at: 6241 6241 Size: 6247 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2089 2373 3475 4054 Size: 53 83 186 936 556 284 1102 579 Cuts at: 4054 4436 5071 275 Size: 382 635 1451 Fragments arranged by size: 1451 1102 936 635 579 556 382 284 186 83 53 BsaWI w'CCGG_w Cuts at: 4680 5078 5352 5499 5910 4680 Size: 398 274 147 411 5017 Fragments arranged by size: 5017 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2510 2540 2874 2904 1178 Size: 30 165 30 1107 30 334 30 4521 Fragments arranged by size: 4521 1107 334 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2515 2518 2617 2939 3094 1186 Size: 1329 3 99 322 155 4339 Fragments arranged by size: 4339 1329 322 155 99 3 BseYI C'CCAG_C Cuts at: 2029 2362 2442 3189 3196 3210 3458 5450 5946 Size: 333 80 747 7 14 248 1992 496 Cuts at: 5946 2029 Size: 2330 Fragments arranged by size: 2330 1992 747 496 333 248 80 14 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2912 3029 1163 Size: 573 1176 117 4381 Fragments arranged by size: 4381 1176 573 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2622 2706 3697 4458 5486 1540 Size: 275 186 621 84 991 761 1028 2301 Fragments arranged by size: 2301 1028 991 761 621 275 186 84 BsiHKAI G_wGCw'C Cuts at: 2103 2126 2699 3086 4308 4393 5464 2103 Size: 23 573 387 1222 85 1071 2886 Fragments arranged by size: 2886 1222 1071 573 387 85 23 BsmI GAATG_Cn' Cuts at: 3758 3851 3758 Size: 93 6154 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2103 2126 2587 2699 2826 3086 Size: 399 246 67 23 461 112 127 260 Cuts at: 3086 3243 3566 4308 4393 5464 1391 Size: 157 323 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 461 399 323 260 246 157 127 112 85 67 23 BspEI T'CCGG_A Cuts at: 5910 5910 Size: 6247 BspHI T'CATG_A Cuts at: 4136 5866 4136 Size: 1730 4517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2428 3220 3602 844 Size: 1584 792 382 3489 Fragments arranged by size: 3489 1584 792 382 BsrBI CCG'CTC Cuts at: 1394 1823 2761 4134 1394 Size: 429 938 1373 3507 Fragments arranged by size: 3507 1373 938 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2795 4742 4916 65 Size: 1183 1547 1947 174 1396 Fragments arranged by size: 1947 1547 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2564 3114 4891 5078 1700 Size: 110 754 550 1777 187 2869 Fragments arranged by size: 2869 1777 754 550 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6247 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6247 BssSI C'ACGA_G Cuts at: 4307 5319 4307 Size: 1012 5235 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6247 BstBI TT'CG_AA Cuts at: 5051 5051 Size: 6247 BstEII G'GTnAC_C Cuts at: 1184 3110 1184 Size: 1926 4321 BstXI CCAn_nnnn'nTGG Cuts at: 3305 3305 Size: 6247 BstYI r'GATC_y Cuts at: 1972 2541 3380 3577 3944 4329 4346 5798 5884 Size: 569 839 197 367 385 17 1452 86 Cuts at: 5884 5896 6242 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 839 569 385 367 346 197 86 17 12 BstZ17I GTA'TAC Cuts at: 2417 2417 Size: 6247 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4756 Fragments arranged by size: 4756 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5508 Fragments arranged by size: 5508 683 56 BtsI GCAGTG_nn' Cuts at: 3125 3200 3418 3763 4557 4577 3125 Size: 75 218 345 794 20 4795 Fragments arranged by size: 4795 794 345 218 75 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6247 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6212 DraI TTT'AAA Cuts at: 3551 3551 Size: 6247 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5254 809 Size: 507 3938 1802 Fragments arranged by size: 3938 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2272 2574 Size: 54 1425 50 275 18 177 265 302 Cuts at: 2574 3295 3694 4583 6212 8 Size: 721 399 889 1629 43 Fragments arranged by size: 1629 1425 889 721 399 302 275 265 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3694 1537 Size: 275 1882 4090 Fragments arranged by size: 4090 1882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3402 4177 1353 Size: 2049 775 3423 Fragments arranged by size: 3423 2049 775 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2620 2864 3455 4834 5208 5354 Size: 1064 138 1031 244 591 1379 374 146 Cuts at: 5354 387 Size: 1280 Fragments arranged by size: 1379 1280 1064 1031 591 374 244 146 138 EcoICRI GAG'CTC Cuts at: 2124 2124 Size: 6247 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2210 2445 2515 2612 2738 2772 Size: 393 423 155 235 70 97 126 34 Cuts at: 2772 4310 4907 5694 5962 1239 Size: 1538 597 787 268 1524 Fragments arranged by size: 1538 1524 787 597 423 393 268 235 155 126 97 70 34 EcoNI CCTnn'n_nnAGG Cuts at: 2424 2424 Size: 6247 EcoO109I rG'GnC_Cy Cuts at: 2033 2134 2712 2823 3444 2033 Size: 101 578 111 621 4836 Fragments arranged by size: 4836 621 578 111 101 EcoRI G'AATT_C Cuts at: 3556 3934 3556 Size: 378 5869 FspI TGC'GCA Cuts at: 4753 4753 Size: 6247 HaeII r_GCGC'y Cuts at: 1678 2092 2376 2817 2867 2918 3478 5394 1678 Size: 414 284 441 50 51 560 1916 2531 Fragments arranged by size: 2531 1916 560 441 414 284 51 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2329 2361 2471 Size: 32 135 32 641 32 84 32 110 Cuts at: 2471 2503 2663 2695 2934 2966 4888 4920 4962 Size: 32 160 32 239 32 1922 32 42 Cuts at: 4962 4994 1373 Size: 32 2626 Fragments arranged by size: 2626 1922 641 239 160 135 110 84 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3591 3837 669 Size: 988 281 1653 246 3079 Fragments arranged by size: 3079 1653 988 281 246 HindIII A'AGCT_T Cuts at: 748 3046 748 Size: 2298 3949 HpaI GTT'AAC Cuts at: 3837 3837 Size: 6247 KasI G'GCGC_C Cuts at: 2088 2372 3474 2088 Size: 284 1102 4861 Fragments arranged by size: 4861 1102 284 KpnI G_GTAC'C Cuts at: 3572 3572 Size: 6247 MfeI C'AATT_G Cuts at: 3846 3846 Size: 6247 MluI A'CGCG_T Cuts at: 6123 6123 Size: 6247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 5361 5545 1305 Size: 939 3117 184 2007 Fragments arranged by size: 3117 2007 939 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2274 3297 10 Size: 54 1425 343 442 1023 2960 Fragments arranged by size: 2960 1425 1023 442 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4205 4564 4723 518 Size: 648 3039 359 159 2042 Fragments arranged by size: 3039 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3076 3663 4340 5488 5733 1606 Size: 1470 587 677 1148 245 2120 Fragments arranged by size: 2120 1470 1148 677 587 245 NaeI GCC'GGC Cuts at: 2566 2566 Size: 6247 NarI GG'CG_CC Cuts at: 2089 2373 3475 2089 Size: 284 1102 4861 Fragments arranged by size: 4861 1102 284 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4756 Fragments arranged by size: 4756 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6247 NgoMIV G'CCGG_C Cuts at: 2564 2564 Size: 6247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6247 NotI GC'GGCC_GC Cuts at: 3694 3694 Size: 6247 NspI r_CATG'y Cuts at: 1132 1229 3138 3356 3605 5150 1132 Size: 97 1909 218 249 1545 2229 Fragments arranged by size: 2229 1909 1545 249 218 97 PasI CC'CwG_GG Cuts at: 1348 2131 2827 3305 1348 Size: 783 696 478 4290 Fragments arranged by size: 4290 783 696 478 PciI A'CATG_T Cuts at: 3134 5146 3134 Size: 2012 4235 PflMI CCAn_nnn'nTGG Cuts at: 1108 2029 2137 2538 3268 3424 1108 Size: 921 108 401 730 156 3931 Fragments arranged by size: 3931 921 730 401 156 108 PmeI GTTT'AAAC Cuts at: 3551 3551 Size: 6247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4316 4348 5829 5861 4316 Size: 32 1481 32 4702 Fragments arranged by size: 4702 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2712 2712 Size: 6247 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6247 PsiI TTA'TAA Cuts at: 3817 3817 Size: 6247 PspOMI G'GGCC_C Cuts at: 2032 2583 2822 2032 Size: 551 239 5457 Fragments arranged by size: 5457 551 239 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3272 3304 3272 Size: 32 6215 PstI C_TGCA'G Cuts at: 830 2167 2871 3599 830 Size: 1337 704 728 3478 Fragments arranged by size: 3478 1337 728 704 PvuI CG_AT'CG Cuts at: 2001 2706 2001 Size: 705 5542 PvuII CAG'CTG Cuts at: 3076 3076 Size: 6247 SacI G_AGCT'C Cuts at: 2126 2126 Size: 6247 SalI G'TCGA_C Cuts at: 1936 3589 1936 Size: 1653 4594 SanDI GG'GwC_CC Cuts at: 2712 2712 Size: 6247 SbfI CC_TGCA'GG Cuts at: 3599 3599 Size: 6247 ScaI AGT'ACT Cuts at: 4495 6207 4495 Size: 1712 4535 SfcI C'TryA_G Cuts at: 826 1045 2163 2237 2867 3497 3595 4730 5411 Size: 219 1118 74 630 630 98 1135 681 Cuts at: 5411 5602 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1118 681 630 630 219 191 98 74 SfiI GGCCn_nnn'nGGCC Cuts at: 2582 2582 Size: 6247 SfoI GGC'GCC Cuts at: 2090 2374 3476 2090 Size: 284 1102 4861 Fragments arranged by size: 4861 1102 284 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6247 SmaI CCC'GGG Cuts at: 1872 3574 1872 Size: 1702 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2787 3509 4351 5252 5514 820 Size: 197 926 844 722 842 901 262 1553 Fragments arranged by size: 1553 926 901 844 842 722 262 197 SnaBI TAC'GTA Cuts at: 493 4038 493 Size: 3545 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6247 SphI G_CATG'C Cuts at: 1132 3605 1132 Size: 2473 3774 SspI AAT'ATT Cuts at: 5 52 4171 5 Size: 47 4119 2081 Fragments arranged by size: 4119 2081 47 StuI AGG'CCT Cuts at: 1517 2430 1517 Size: 913 5334 StyI C'CwwG_G Cuts at: 513 1065 2004 2317 2355 2571 3469 3686 513 Size: 552 939 313 38 216 898 217 3074 Fragments arranged by size: 3074 939 898 552 313 217 216 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 2239 2608 3098 4274 4291 4444 Size: 1255 504 155 369 490 1176 17 153 Cuts at: 4444 4629 5074 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1176 504 490 445 369 185 155 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4493 6205 96 Size: 274 80 33 51 1443 2516 1712 138 Fragments arranged by size: 2516 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2874 2906 6207 6239 2874 Size: 32 3301 32 2882 Fragments arranged by size: 3301 2882 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6247 XbaI T'CTAG_A Cuts at: 3583 3583 Size: 6247 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2181 2267 2181 Size: 86 6161 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6247 XmaI C'CCGG_G Cuts at: 1870 3572 1870 Size: 1702 4545 XmnI GAAnn'nnTTC Cuts at: 3485 4376 3485 Size: 891 5356 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4055 276 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BsmBI Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI RsrII SacII SapI SexAI SrfI SwaI