(Linear) (Six-base) MAPSORT of: KIEE1958.seq Check: 1827 from: 1 to: 1542 KIEE1958.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1215 1542 Size: 1215 327 AccI GT'mk_AC Cuts at: 0 411 1542 Size: 411 1131 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 767 1542 Size: 607 160 775 Fragments arranged by size: 775 607 160 AfeI AGC'GCT Cuts at: 0 810 1542 Size: 810 732 AflIII A'CryG_T Cuts at: 0 209 1129 1542 Size: 209 920 413 Fragments arranged by size: 920 413 209 AlwNI CAG_nnn'CTG Cuts at: 0 24 132 157 533 870 1542 Size: 24 108 25 376 337 672 Fragments arranged by size: 672 376 337 108 25 24 ApaI G_GGCC'C Cuts at: 0 31 582 821 1542 Size: 31 551 239 721 Fragments arranged by size: 721 551 239 31 AvaI C'yCGr_G Cuts at: 0 768 946 1049 1542 Size: 768 178 103 493 Fragments arranged by size: 768 493 178 103 AvrII C'CTAG_G Cuts at: 0 1464 1542 Size: 1464 78 BamHI G'GATC_C Cuts at: 0 1375 1542 Size: 1375 167 BanI G'GyrC_C Cuts at: 0 83 367 1428 1469 1542 Size: 83 284 1061 41 73 Fragments arranged by size: 1061 284 83 73 41 BanII G_rGCy'C Cuts at: 0 31 121 582 821 1238 1542 Size: 31 90 461 239 417 304 Fragments arranged by size: 461 417 304 239 90 31 BbeI G_GCGC'C Cuts at: 0 87 371 1473 1542 Size: 87 284 1102 69 Fragments arranged by size: 1102 284 87 69 BbvCI CC'TCA_GC Cuts at: 0 1072 1542 Size: 1072 470 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 681 715 914 948 1542 Size: 681 34 199 34 594 Fragments arranged by size: 681 594 199 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 684 1542 Size: 684 858 BglI GCCn_nnn'nGGC Cuts at: 0 493 577 1542 Size: 493 84 965 Fragments arranged by size: 965 493 84 BlpI GC'TnA_GC Cuts at: 0 805 916 1542 Size: 805 111 626 Fragments arranged by size: 805 626 111 Bme1580I G_kGCm'C Cuts at: 0 31 582 821 1542 Size: 31 551 239 721 Fragments arranged by size: 721 551 239 31 BmgBI CAC'GTC Cuts at: 0 669 1542 Size: 669 873 BmrI ACTGGGnnnn_n' Cuts at: 0 12 252 1542 Size: 12 240 1290 Fragments arranged by size: 1290 240 12 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1458 1542 Size: 1458 84 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 205 440 510 733 1542 Size: 50 155 235 70 223 809 Fragments arranged by size: 809 235 223 155 70 50 Bpu10I CC'TnA_GC Cuts at: 0 139 1072 1542 Size: 139 933 470 Fragments arranged by size: 933 470 139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 767 1489 1542 Size: 767 722 53 Fragments arranged by size: 767 722 53 BsaI GGTCTCn'nnnn_ Cuts at: 0 486 1542 Size: 486 1056 BsaHI Gr'CG_yC Cuts at: 0 84 368 1470 1542 Size: 84 284 1102 72 Fragments arranged by size: 1102 284 84 72 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 505 535 869 899 1542 Size: 505 30 334 30 643 Fragments arranged by size: 643 505 334 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 510 513 612 934 1089 1542 Size: 510 3 99 322 155 453 Fragments arranged by size: 510 453 322 155 99 3 BseYI C'CCAG_C Cuts at: 0 24 357 437 1184 1191 1205 1453 1542 Size: 24 333 80 747 7 14 248 89 Fragments arranged by size: 747 333 248 89 80 24 14 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 907 1024 1542 Size: 907 117 518 Fragments arranged by size: 907 518 117 BsiEI CG_ry'CG Cuts at: 0 617 701 1542 Size: 617 84 841 Fragments arranged by size: 841 617 84 BsiHKAI G_wGCw'C Cuts at: 0 98 121 694 1081 1542 Size: 98 23 573 387 461 Fragments arranged by size: 573 461 387 98 23 Bsp1286I G_dGCh'C Cuts at: 0 31 98 121 582 694 821 1081 1238 Size: 31 67 23 461 112 127 260 157 Cuts at: 1238 1542 Size: 304 Fragments arranged by size: 461 304 260 157 127 112 67 31 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 423 1215 1542 Size: 423 792 327 Fragments arranged by size: 792 423 327 BsrBI CCG'CTC Cuts at: 0 756 1542 Size: 756 786 BsrDI GCAATG_nn' Cuts at: 0 790 1542 Size: 790 752 BsrFI r'CCGG_y Cuts at: 0 559 1109 1542 Size: 559 550 433 Fragments arranged by size: 559 550 433 BstEII G'GTnAC_C Cuts at: 0 1105 1542 Size: 1105 437 BstXI CCAn_nnnn'nTGG Cuts at: 0 1300 1542 Size: 1300 242 BstYI r'GATC_y Cuts at: 0 536 1375 1542 Size: 536 839 167 Fragments arranged by size: 839 536 167 BstZ17I GTA'TAC Cuts at: 0 412 1542 Size: 412 1130 BtsI GCAGTG_nn' Cuts at: 0 1120 1195 1413 1542 Size: 1120 75 218 129 Fragments arranged by size: 1120 218 129 75 EaeI y'GGCC_r Cuts at: 0 2 267 569 1290 1542 Size: 2 265 302 721 252 Fragments arranged by size: 721 302 265 252 2 EarI CTCTTCn'nnn_ Cuts at: 0 1397 1542 Size: 1397 145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 615 859 1450 1542 Size: 615 244 591 92 Fragments arranged by size: 615 591 244 92 EcoICRI GAG'CTC Cuts at: 0 119 1542 Size: 119 1423 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 205 440 510 607 733 767 1542 Size: 50 155 235 70 97 126 34 775 Fragments arranged by size: 775 235 155 126 97 70 50 34 EcoNI CCTnn'n_nnAGG Cuts at: 0 419 1542 Size: 419 1123 EcoO109I rG'GnC_Cy Cuts at: 0 28 129 707 818 1439 1542 Size: 28 101 578 111 621 103 Fragments arranged by size: 621 578 111 103 101 28 HaeII r_GCGC'y Cuts at: 0 87 371 812 862 913 1473 1542 Size: 87 284 441 50 51 560 69 Fragments arranged by size: 560 441 284 87 69 51 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 208 240 324 356 466 498 658 690 Size: 208 32 84 32 110 32 160 32 Cuts at: 690 929 961 1542 Size: 239 32 581 Fragments arranged by size: 581 239 208 160 110 84 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1041 1542 Size: 1041 501 KasI G'GCGC_C Cuts at: 0 83 367 1469 1542 Size: 83 284 1102 73 Fragments arranged by size: 1102 284 83 73 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 239 1542 Size: 239 1303 MscI TGG'CCA Cuts at: 0 269 1292 1542 Size: 269 1023 250 Fragments arranged by size: 1023 269 250 MspA1I CmG'CkG Cuts at: 0 1071 1542 Size: 1071 471 NaeI GCC'GGC Cuts at: 0 561 1542 Size: 561 981 NarI GG'CG_CC Cuts at: 0 84 368 1470 1542 Size: 84 284 1102 72 Fragments arranged by size: 1102 284 84 72 NgoMIV G'CCGG_C Cuts at: 0 559 1542 Size: 559 983 NspI r_CATG'y Cuts at: 0 1133 1351 1542 Size: 1133 218 191 Fragments arranged by size: 1133 218 191 PasI CC'CwG_GG Cuts at: 0 126 822 1300 1542 Size: 126 696 478 242 Fragments arranged by size: 696 478 242 126 PciI A'CATG_T Cuts at: 0 1129 1542 Size: 1129 413 PflMI CCAn_nnn'nTGG Cuts at: 0 24 132 533 1263 1419 1542 Size: 24 108 401 730 156 123 Fragments arranged by size: 730 401 156 123 108 24 PpuMI rG'GwC_Cy Cuts at: 0 707 1542 Size: 707 835 PspOMI G'GGCC_C Cuts at: 0 27 578 817 1542 Size: 27 551 239 725 Fragments arranged by size: 725 551 239 27 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1267 1299 1542 Size: 1267 32 243 Fragments arranged by size: 1267 243 32 PstI C_TGCA'G Cuts at: 0 162 866 1542 Size: 162 704 676 Fragments arranged by size: 704 676 162 PvuI CG_AT'CG Cuts at: 0 701 1542 Size: 701 841 PvuII CAG'CTG Cuts at: 0 1071 1542 Size: 1071 471 SacI G_AGCT'C Cuts at: 0 121 1542 Size: 121 1421 SanDI GG'GwC_CC Cuts at: 0 707 1542 Size: 707 835 SfcI C'TryA_G Cuts at: 0 158 232 862 1492 1542 Size: 158 74 630 630 50 Fragments arranged by size: 630 630 158 74 50 SfiI GGCCn_nnn'nGGCC Cuts at: 0 577 1542 Size: 577 965 SfoI GGC'GCC Cuts at: 0 85 369 1471 1542 Size: 85 284 1102 71 Fragments arranged by size: 1102 284 85 71 SmlI C'TyrA_G Cuts at: 0 782 1504 1542 Size: 782 722 38 Fragments arranged by size: 782 722 38 StuI AGG'CCT Cuts at: 0 425 1542 Size: 425 1117 StyI C'CwwG_G Cuts at: 0 312 350 566 1464 1542 Size: 312 38 216 898 78 Fragments arranged by size: 898 312 216 78 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 79 234 603 1093 1542 Size: 79 155 369 490 449 Fragments arranged by size: 490 449 369 155 79 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 869 901 1542 Size: 869 32 641 Fragments arranged by size: 869 641 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 176 262 1542 Size: 176 86 1280 Fragments arranged by size: 1280 176 86 XmnI GAAnn'nnTTC Cuts at: 0 1480 1542 Size: 1480 62 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AlwNI ApaI AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI BstZ17I BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI MmeI MscI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PpuMI PspOMI PsrI PstI PvuI PvuII SacI SanDI SfcI SfiI SfoI SmlI StuI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI BaeI BbsI BclI BfrBI BglII BmtI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI MslI NcoI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspXI RsrII SacII SalI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI ZraI