(Linear) (Six-base) MAPSORT of: KIEE2030.seq Check: 8019 from: 1 to: 1860 KIEE2030.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AccI GT'mk_AC Cuts at: 0 482 1860 Size: 482 1378 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1155 1610 1860 Size: 1155 455 250 Fragments arranged by size: 1155 455 250 AleI CACnn'nnGTG Cuts at: 0 1683 1860 Size: 1683 177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 249 1860 Size: 249 1611 AlwNI CAG_nnn'CTG Cuts at: 0 359 1413 1860 Size: 359 1054 447 Fragments arranged by size: 1054 447 359 ApoI r'AATT_y Cuts at: 0 1699 1860 Size: 1699 161 BanI G'GyrC_C Cuts at: 0 233 1860 Size: 233 1627 BanII G_rGCy'C Cuts at: 0 293 1860 Size: 293 1567 BbsI GAAGACnn'nnnn_ Cuts at: 0 368 391 1205 1663 1860 Size: 368 23 814 458 197 Fragments arranged by size: 814 458 368 197 23 BbvCI CC'TCA_GC Cuts at: 0 1385 1860 Size: 1385 475 BfrBI ATG'CAT Cuts at: 0 1215 1860 Size: 1215 645 BmrI ACTGGGnnnn_n' Cuts at: 0 369 1860 Size: 369 1491 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 336 1751 1860 Size: 336 1415 109 Fragments arranged by size: 1415 336 109 Bpu10I CC'TnA_GC Cuts at: 0 568 1385 1860 Size: 568 817 475 Fragments arranged by size: 817 568 475 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1006 1740 1860 Size: 1006 734 120 Fragments arranged by size: 1006 734 120 BsaBI GATnn'nnATC Cuts at: 0 881 1860 Size: 881 979 BsaWI w'CCGG_w Cuts at: 0 1194 1860 Size: 1194 666 BsiHKAI G_wGCw'C Cuts at: 0 66 257 1049 1860 Size: 66 191 792 811 Fragments arranged by size: 811 792 191 66 Bsp1286I G_dGCh'C Cuts at: 0 66 257 293 1049 1860 Size: 66 191 36 756 811 Fragments arranged by size: 811 756 191 66 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1011 1860 Size: 1011 849 BsrBI CCG'CTC Cuts at: 0 12 1860 Size: 12 1848 BsrDI GCAATG_nn' Cuts at: 0 1187 1560 1860 Size: 1187 373 300 Fragments arranged by size: 1187 373 300 BsrGI T'GTAC_A Cuts at: 0 807 1574 1860 Size: 807 767 286 Fragments arranged by size: 807 767 286 BssSI C'ACGA_G Cuts at: 0 98 1860 Size: 98 1762 BstYI r'GATC_y Cuts at: 0 345 780 1860 Size: 345 435 1080 Fragments arranged by size: 1080 435 345 BtgI C'CryG_G Cuts at: 0 1684 1860 Size: 1684 176 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1072 1860 Size: 1072 788 BtsI GCAGTG_nn' Cuts at: 0 917 1860 Size: 917 943 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 966 1001 1860 Size: 966 35 859 Fragments arranged by size: 966 859 35 DraI TTT'AAA Cuts at: 0 741 1860 Size: 741 1119 DrdI GACnn_nn'nnGTC Cuts at: 0 1842 1860 Size: 1842 18 EarI CTCTTCn'nnn_ Cuts at: 0 281 1860 Size: 281 1579 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 336 1155 1610 1751 1860 Size: 336 819 455 141 109 Fragments arranged by size: 819 455 336 141 109 EcoO109I rG'GnC_Cy Cuts at: 0 264 1860 Size: 264 1596 EcoRV GAT'ATC Cuts at: 0 964 1860 Size: 964 896 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 568 1860 Size: 568 1292 HincII GTy'rAC Cuts at: 0 1323 1860 Size: 1323 537 HindIII A'AGCT_T Cuts at: 0 464 472 754 1592 1860 Size: 464 8 282 838 268 Fragments arranged by size: 838 464 282 268 8 MfeI C'AATT_G Cuts at: 0 1301 1860 Size: 1301 559 MslI CAynn'nnrTG Cuts at: 0 944 1570 1683 1860 Size: 944 626 113 177 Fragments arranged by size: 944 626 177 113 MspA1I CmG'CkG Cuts at: 0 1086 1860 Size: 1086 774 NsiI A_TGCA'T Cuts at: 0 1217 1860 Size: 1217 643 NspI r_CATG'y Cuts at: 0 215 525 955 1360 1860 Size: 215 310 430 405 500 Fragments arranged by size: 500 430 405 310 215 PflMI CCAn_nnn'nTGG Cuts at: 0 359 1059 1860 Size: 359 700 801 Fragments arranged by size: 801 700 359 PsiI TTA'TAA Cuts at: 0 728 1860 Size: 728 1132 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 429 461 1860 Size: 429 32 1399 Fragments arranged by size: 1399 429 32 PstI C_TGCA'G Cuts at: 0 1366 1725 1860 Size: 1366 359 135 Fragments arranged by size: 1366 359 135 PvuII CAG'CTG Cuts at: 0 1086 1860 Size: 1086 774 SapI GCTCTTCn'nnn_ Cuts at: 0 281 1860 Size: 281 1579 SfcI C'TryA_G Cuts at: 0 450 668 764 1362 1721 1860 Size: 450 218 96 598 359 139 Fragments arranged by size: 598 450 359 218 139 96 SmlI C'TyrA_G Cuts at: 0 985 1755 1860 Size: 985 770 105 Fragments arranged by size: 985 770 105 StuI AGG'CCT Cuts at: 0 1390 1860 Size: 1390 470 StyI C'CwwG_G Cuts at: 0 1391 1515 1784 1860 Size: 1391 124 269 76 Fragments arranged by size: 1391 269 124 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 170 925 1860 Size: 170 755 935 Fragments arranged by size: 935 755 170 TatI w'GTAC_w Cuts at: 0 807 1574 1860 Size: 807 767 286 Fragments arranged by size: 807 767 286 XbaI T'CTAG_A Cuts at: 0 426 1860 Size: 426 1434 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1513 1782 1860 Size: 1513 269 78 Fragments arranged by size: 1513 269 78 Enzymes that do cut: AccI AcuI AleI AlfI AlwNI ApoI BanI BanII BbsI BbvCI BfrBI BmrI BpmI Bpu10I BpuEI BsaBI BsaWI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EarI Eco57MI EcoO109I EcoRV FalI HincII HindIII MfeI MslI MspA1I NsiI NspI PflMI PsiI PsrI PstI PvuII SapI SfcI SmlI StuI StyI TaqII TatI XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraIII EaeI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII Hin4I HpaI KasI KpnI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XhoI XmaI XmnI ZraI