Restriction map for pFN21AE0311
 (Linear) (Six-base) MAPSORT of: KIEE0311.seq  Check: 5727  from: 1  to: 3795



KIEE0311.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2307    3795 
   Size:       2307    1488
 
AatII G_ACGT'C

Cuts at:      0    1691    3229    3795 
   Size:       1691    1538     566

  Fragments arranged by size: 

                1691    1538     566
 
AccI GT'mk_AC

Cuts at:      0    3319    3795 
   Size:       3319     476
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     554    2192    3701    3795 
   Size:        554    1638    1509      94

  Fragments arranged by size: 

                1638    1509     554      94
 
AflIII A'CryG_T

Cuts at:      0    1883    2793    3795 
   Size:       1883     910    1002

  Fragments arranged by size: 

                1883    1002     910
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     469    3795 
   Size:        469    3326
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3094    3795 
   Size:       3094     701
 
ApoI r'AATT_y

Cuts at:      0      71     210     267    1534    1552    1569    1699    1737 
   Size:         71     139      57    1267      18      17     130      38

Cuts at:   1737    2581    2619    2924    3522    3592    3795 
   Size:        844      38     305     598      70     203

  Fragments arranged by size: 

                1267     844     598     305     203     139     130      71
                  70      57      38      38      18      17
 
AseI AT'TA_AT

Cuts at:      0    1826    3795 
   Size:       1826    1969
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     451    1861    2161    2282    2743    3253    3795 
   Size:        451    1410     300     121     461     510     542

  Fragments arranged by size: 

                1410     542     510     461     451     300     121
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1107    1141    3795 
   Size:       1107      34    2654

  Fragments arranged by size: 

                2654    1107      34
 
BclI T'GATC_A

Cuts at:      0    2919    3795 
   Size:       2919     876
 
BglII A'GATC_T

Cuts at:      0    1303    2817    3162    3409    3708    3795 
   Size:       1303    1514     345     247     299      87

  Fragments arranged by size: 

                1514    1303     345     299     247      87
 
BlpI GC'TnA_GC

Cuts at:      0    2687    3795 
   Size:       2687    1108
 
Bme1580I G_kGCm'C

Cuts at:      0      10    3795 
   Size:         10    3785
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       4     759    3103    3795 
   Size:          4     755    2344     692

  Fragments arranged by size: 

                2344     755     692       4
 
BmtI G_CTAG'C

Cuts at:      0     419    3795 
   Size:        419    3376
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3251    3795 
   Size:       3251     544
 
Bpu10I CC'TnA_GC

Cuts at:      0     185    1394    2134    2716    3795 
   Size:        185    1209     740     582    1079

  Fragments arranged by size: 

                1209    1079     740     582     185
 
BsaBI GATnn'nnATC

Cuts at:      0    3095    3795 
   Size:       3095     700
 
BsaHI Gr'CG_yC

Cuts at:      0    1688    3226    3795 
   Size:       1688    1538     569

  Fragments arranged by size: 

                1688    1538     569
 
BsaWI w'CCGG_w

Cuts at:      0    1850    3605    3795 
   Size:       1850    1755     190

  Fragments arranged by size: 

                1850    1755     190
 
BseYI C'CCAG_C

Cuts at:      0    2007    3543    3795 
   Size:       2007    1536     252

  Fragments arranged by size: 

                2007    1536     252
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1352    3795 
   Size:       1352    2443
 
BsiHKAI G_wGCw'C

Cuts at:      0    3541    3795 
   Size:       3541     254
 
BsmI GAATG_Cn'

Cuts at:      0    2863    3795 
   Size:       2863     932
 
Bsp1286I G_dGCh'C

Cuts at:      0      10    3541    3795 
   Size:         10    3531     254

  Fragments arranged by size: 

                3531     254      10
 
BspHI T'CATG_A

Cuts at:      0     338    3795 
   Size:        338    3457
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2307    2394    3795 
   Size:       2307      87    1401

  Fragments arranged by size: 

                2307    1401      87
 
BsrGI T'GTAC_A

Cuts at:      0    2496    3795 
   Size:       2496    1299
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     772    3795 
   Size:        772    3023
 
BstYI r'GATC_y

Cuts at:      0     981    1303    1338    2817    3162    3258    3409    3708 
   Size:        981     322      35    1479     345      96     151     299

Cuts at:   3708    3795 
   Size:         87

  Fragments arranged by size: 

                1479     981     345     322     299     151      96      87
                  35
 
Bsu36I CC'TnA_GG

Cuts at:      0      39    3795 
   Size:         39    3756
 
BtsI GCAGTG_nn'

Cuts at:      0     529    3680    3795 
   Size:        529    3151     115

  Fragments arranged by size: 

                3151     529     115
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2301    2336    3795 
   Size:       2301      35    1459

  Fragments arranged by size: 

                2301    1459      35
 
DraI TTT'AAA

Cuts at:      0     161    1251    2829    3068    3597    3762    3795 
   Size:        161    1090    1578     239     529     165      33

  Fragments arranged by size: 

                1578    1090     529     239     165     161      33
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3231    3795 
   Size:       3231     564
 
EaeI y'GGCC_r

Cuts at:      0     774    2370    3795 
   Size:        774    1596    1425

  Fragments arranged by size: 

                1596    1425     774
 
EarI CTCTTCn'nnn_

Cuts at:      0     573    3795 
   Size:        573    3222
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     554    2192    3251    3701    3795 
   Size:        554    1638    1059     450      94

  Fragments arranged by size: 

                1638    1059     554     450      94
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1754    2051    3795 
   Size:       1754     297    1744

  Fragments arranged by size: 

                1754    1744     297
 
EcoRI G'AATT_C

Cuts at:      0     210    1569    3795 
   Size:        210    1359    2226

  Fragments arranged by size: 

                2226    1359     210
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1613    3405    3795 
   Size:       1613    1792     390

  Fragments arranged by size: 

                1792    1613     390
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     550     582     778     810     921     953    1198    1230 
   Size:        550      32     196      32     111      32     245      32

Cuts at:   1230    1295    1327    2665    2697    3795 
   Size:         65      32    1338      32    1098

  Fragments arranged by size: 

                1338    1098     550     245     196     111      65      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2895    3026    3603    3795 
   Size:       2895     131     577     192

  Fragments arranged by size: 

                2895     577     192     131
 
HindIII A'AGCT_T

Cuts at:      0     316    1595    1831    2131    3795 
   Size:        316    1279     236     300    1664

  Fragments arranged by size: 

                1664    1279     316     300     236
 
HpaI GTT'AAC

Cuts at:      0    3026    3795 
   Size:       3026     769
 
MfeI C'AATT_G

Cuts at:      0    1063    3795 
   Size:       1063    2732
 
MscI TGG'CCA

Cuts at:      0    2372    3795 
   Size:       2372    1423
 
MslI CAynn'nnrTG

Cuts at:      0     944    1886    2089    2372    3795 
   Size:        944     942     203     283    1423

  Fragments arranged by size: 

                1423     944     942     283     203
 
MspA1I CmG'CkG

Cuts at:      0    1368    1389    3795 
   Size:       1368      21    2406

  Fragments arranged by size: 

                2406    1368      21
 
NheI G'CTAG_C

Cuts at:      0     415    3795 
   Size:        415    3380
 
NspI r_CATG'y

Cuts at:      0    1887    2797    3795 
   Size:       1887     910     998

  Fragments arranged by size: 

                1887     998     910
 
PciI A'CATG_T

Cuts at:      0    1883    2793    3795 
   Size:       1883     910    1002

  Fragments arranged by size: 

                1883    1002     910
 
PfoI T'CCnGG_A

Cuts at:      0    1313    3099    3795 
   Size:       1313    1786     696

  Fragments arranged by size: 

                1786    1313     696
 
PpuMI rG'GwC_Cy

Cuts at:      0    1754    2051    3795 
   Size:       1754     297    1744

  Fragments arranged by size: 

                1754    1744     297
 
PstI C_TGCA'G

Cuts at:      0     861    3795 
   Size:        861    2934
 
PvuII CAG'CTG

Cuts at:      0    1368    1389    3795 
   Size:       1368      21    2406

  Fragments arranged by size: 

                2406    1368      21
 
SanDI GG'GwC_CC

Cuts at:      0    1754    3795 
   Size:       1754    2041
 
SfcI C'TryA_G

Cuts at:      0     680     857    2046    3795 
   Size:        680     177    1189    1749

  Fragments arranged by size: 

                1749    1189     680     177
 
SpeI A'CTAG_T

Cuts at:      0    3079    3795 
   Size:       3079     716
 
SspI AAT'ATT

Cuts at:      0     537    3795 
   Size:        537    3258
 
StyI C'CwwG_G

Cuts at:      0    3530    3795 
   Size:       3530     265
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2653    3795 
   Size:       2653    1142
 
TatI w'GTAC_w

Cuts at:      0    1458    2496    3745    3795 
   Size:       1458    1038    1249      50

  Fragments arranged by size: 

                1458    1249    1038      50
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     810     842    3795 
   Size:        810      32    2953

  Fragments arranged by size: 

                2953     810      32
 
XbaI T'CTAG_A

Cuts at:      0    1600    3795 
   Size:       1600    2195
 
XmnI GAAnn'nnTTC

Cuts at:      0    1189    2453    3795 
   Size:       1189    1264    1342

  Fragments arranged by size: 

                1342    1264    1189
 
ZraI GAC'GTC

Cuts at:      0    1689    3227    3795 
   Size:       1689    1538     568

  Fragments arranged by size: 

                1689    1538     568
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AlfI    AlwNI     ApoI
     AseI     BbsI     BcgI     BclI    BglII     BlpI Bme1580I     BmrI
     BmtI     BpmI   Bpu10I    BsaBI    BsaHI    BsaWI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrGI    BstXI    BstYI
   Bsu36I     BtsI    CspCI     DraI     DrdI     EaeI     EarI  Eco57MI
 EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     HpaI     MfeI
     MscI     MslI   MspA1I     NheI     NspI     PciI     PfoI    PpuMI
     PstI    PvuII    SanDI     SfcI     SpeI     SspI     StyI    TaqII
     TatI     TstI     XbaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI     AloI
     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI    BciVI    BfrBI     BglI    BmgBI
     BplI    BpuEI     BsaI    BsaAI    BsaXI    BseRI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I     BtgI    BtgZI     ClaI   DraIII     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI
     SrfI     StuI     SwaI  Tth111I     XcmI     XhoI     XmaI
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