################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TspRI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AlwI 723 GGATC GGATC(4/5) AvaII 62 GGTCC G^G[AT]CC BanI 838 GGTGCC G^G[CT][GA]CC BanII 737 GAGCCC G[GA]GC[CT]^C BbsI 596 GTCTTC GAAGAC(2/6) BfaI 157 CTAG C^TAG BglII 534 AGATCT A^GATCT BmyI 737 GAGCCC G[AGT]GC[ACT]^C BpuAI 596 GTCTTC GAAGAC(2/6) BsaBI 718 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 921 GAGACC GGTCTC(1/5) BseRI 662 GAGGAG GAGGAG(10/8) BsgI 683 GTGCAG GTGCAG(16/14) BsmBI 304 GAGACG CGTCTC(1/5) BsmFI 272 GGGAC GGGAC(10/14) Bsp1286I 737 GAGCCC G[AGT]GC[ACT]^C BspMI 835 GCAGGT ACCTGC(4/8) BsrI 321 ACTGG ACTGG(1/-1) BssKI 70 CCTGG ^CC[ACGT]GG BstNI 70 CCTGG CC^[AT]GG CfoI 805 GCGC GCG^C Cfr13I 62 GGTCC G^G[ACGT]CC DraI 885 TTTAAA TTT^AAA DsaI 195 CCATGG C^C[GA][CT]GG EcoRII 70 CCTGG ^CC[AT]GG HhaI 805 GCGC GCG^C HinP1I 805 GCGC G^CGC HindIII 974 AAGCTT A^AGCTT HinfI 555 GATTC G^A[ACGT]TC MaeI 157 CTAG C^TAG MamI 718 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvaI 70 CCTGG CC^[AT]GG NcoI 195 CCATGG C^CATGG PflMI 741 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 893 CTGCAG CTGCA^G Sau96I 62 GGTCC G^G[ACGT]CC ScrFI 70 CCTGG CC^[ACGT]GG SfcI 893 CTGCAG C^T[GA][CT]AG StuI 951 AGGCCT AGG^CCT TaqI 26 TCGA T^CGA TfiI 555 GATTC G^A[AT]TC TthHB8I 26 TCGA T^CGA Van91I 741 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 842 CCATGATGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 4 GCGG CCGC(-3/-1) 818 GCGG AfaI 231 GTAC GT^AC 583 GTAC AluI 86 AGCT AG^CT 975 AGCT BsaJI 195 CCATGG C^C[ACGT][ACGT]GG 937 CCAAGG BssSI 918 CACGAG CACGAG(-5/-1) 125 CTCGTG BstYI 534 AGATCT [GA]^GATC[CT] 723 GGATCT Cac8I 382 GCCAGC GC[ACGT]^[ACGT]GC 891 GCCTGC EcoT14I 195 CCATGG C^C[AT][AT]GG 937 CCAAGG HaeIII 324 GGCC GG^CC 952 GGCC MflI 534 AGATCT [GA]^GATC[CT] 723 GGATCT MwoI 552 GCAGATTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 561 GCGAATTATGC RsaI 231 GTAC GT^AC 583 GTAC SfaNI 386 GCATC GCATC(5/9) 946 GCATC StyI 195 CCATGG C^C[AT][AT]GG 937 CCAAGG Tsp45I 188 GTGAC ^GT[GC]AC 790 GTCAC XhoII 534 AGATCT [GA]^GATC[CT] 723 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AcsI ApoI Asp700I BbvI BsiYI BslI BsmAI BstF5I DdeI DpnII Fnu4HI FokI HphI ItaI MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII NlaIV Sau3AI Tru9I TseI Tsp509I XmnI