(Circular) (Six-base) MAPSORT of: pFN21AE0303.seq Check: 1452 from: 1 to: 6493 pFN21AE0303 6493 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2059 2059 Size: 6493 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3836 1937 Size: 1899 4594 Acc65I G'GTAC_C Cuts at: 3814 3814 Size: 6493 AclI AA'CG_TT Cuts at: 1563 3792 4620 4993 1563 Size: 2229 828 373 3063 Fragments arranged by size: 3063 2229 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2829 4556 5940 6208 1632 Size: 1197 1727 1384 268 1917 Fragments arranged by size: 1917 1727 1384 1197 268 AfeI AGC'GCT Cuts at: 2999 2999 Size: 6493 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6296 AflIII A'CryG_T Cuts at: 1203 5392 6369 1203 Size: 4189 977 1327 Fragments arranged by size: 4189 1327 977 AgeI A'CCGG_T Cuts at: 5324 5324 Size: 6493 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 2487 2558 2636 1166 Size: 1321 71 78 5023 Fragments arranged by size: 5023 1321 78 71 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3315 1184 Size: 2131 4362 AlwNI CAG_nnn'CTG Cuts at: 3482 4236 5808 3482 Size: 754 1572 4167 Fragments arranged by size: 4167 1572 754 ApaLI G'TGCA_C Cuts at: 3253 4550 5706 3253 Size: 1297 1156 4040 Fragments arranged by size: 4040 1297 1156 ApoI r'AATT_y Cuts at: 1493 3802 4028 4180 6289 1493 Size: 2309 226 152 2109 1697 Fragments arranged by size: 2309 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2744 5047 160 Size: 872 1712 2303 1606 Fragments arranged by size: 2303 1712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 3108 3818 1870 Size: 73 1165 710 4545 Fragments arranged by size: 4545 1165 710 73 BamHI G'GATC_C Cuts at: 3661 3823 4190 3661 Size: 162 367 5964 Fragments arranged by size: 5964 367 162 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2666 3814 5269 618 Size: 325 217 627 273 606 1148 1455 1842 Fragments arranged by size: 1842 1455 1148 627 606 325 273 217 BanII G_rGCy'C Cuts at: 1391 2135 3812 1391 Size: 744 1677 4072 Fragments arranged by size: 4072 1677 744 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5522 BbvCI CC'TCA_GC Cuts at: 2016 2016 Size: 6493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2323 2357 4684 4718 1903 Size: 34 386 34 2327 34 3678 Fragments arranged by size: 3678 2327 386 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 3118 1548 Size: 1570 4923 BfrBI ATG'CAT Cuts at: 3780 3780 Size: 6493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5104 136 Size: 107 122 71 631 605 3432 1525 Fragments arranged by size: 3432 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6488 6488 Size: 6493 BlpI GC'TnA_GC Cuts at: 2904 3910 2904 Size: 1006 5487 Bme1580I G_kGCm'C Cuts at: 1790 2063 2669 3257 3705 4554 5710 1790 Size: 273 606 588 448 849 1156 2573 Fragments arranged by size: 2573 1156 849 606 588 448 273 BmgBI CAC'GTC Cuts at: 1318 2578 1318 Size: 1260 5233 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2312 4224 5182 Size: 416 53 83 399 24 895 1912 958 Cuts at: 5182 442 Size: 1753 Fragments arranged by size: 1912 1753 958 895 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3087 1056 Size: 2031 4462 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3060 3060 Size: 6493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3239 3550 5153 1239 Size: 2000 311 1603 2579 Fragments arranged by size: 2579 2000 1603 311 Bpu10I CC'TnA_GC Cuts at: 2016 2994 3472 2016 Size: 978 478 5037 Fragments arranged by size: 5037 978 478 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4618 5483 5781 4618 Size: 865 298 5330 Fragments arranged by size: 5330 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5156 882 Size: 617 3657 2219 Fragments arranged by size: 3657 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4284 493 Size: 711 3080 2702 Fragments arranged by size: 3080 2702 711 BsaBI GATnn'nnATC Cuts at: 6487 6487 Size: 6493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4300 4682 5317 275 Size: 53 83 186 936 2767 382 635 1451 Fragments arranged by size: 2767 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2227 3126 4926 5324 5598 5745 6156 2227 Size: 899 1800 398 274 147 411 2564 Fragments arranged by size: 2564 1800 899 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2573 2603 1178 Size: 30 165 30 1170 30 5068 Fragments arranged by size: 5068 1170 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 1186 Size: 868 5625 BseYI C'CCAG_C Cuts at: 2908 5696 6192 2908 Size: 2788 496 3209 Fragments arranged by size: 3209 2788 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5920 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2239 2572 3943 4704 5732 1540 Size: 275 186 238 333 1371 761 1028 2301 Fragments arranged by size: 2301 1371 1028 761 333 275 238 186 BsiHKAI G_wGCw'C Cuts at: 2135 2508 3257 4554 4639 5710 2135 Size: 373 749 1297 85 1071 2918 Fragments arranged by size: 2918 1297 1071 749 373 85 BsmI GAATG_Cn' Cuts at: 4004 4097 4004 Size: 93 6400 BsmBI CGTCTCn'nnnn_ Cuts at: 3286 3568 3658 3286 Size: 282 90 6121 Fragments arranged by size: 6121 282 90 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2135 2508 2669 3257 3705 3812 Size: 399 273 72 373 161 588 448 107 Cuts at: 3812 4554 4639 5710 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 588 448 399 373 273 161 107 85 72 BspEI T'CCGG_A Cuts at: 2227 3126 6156 2227 Size: 899 3030 2564 Fragments arranged by size: 3030 2564 899 BspHI T'CATG_A Cuts at: 2196 2373 4382 6112 2196 Size: 177 2009 1730 2577 Fragments arranged by size: 2577 2009 1730 177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2059 3848 844 Size: 1215 1789 3489 Fragments arranged by size: 3489 1789 1215 BsrBI CCG'CTC Cuts at: 1394 1823 4380 1394 Size: 429 2557 3507 Fragments arranged by size: 3507 2557 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2421 2492 3072 4988 5162 65 Size: 1183 1173 71 580 1916 174 1396 Fragments arranged by size: 1916 1396 1183 1173 580 174 71 BsrFI r'CCGG_y Cuts at: 1700 1810 5137 5324 1700 Size: 110 3327 187 2869 Fragments arranged by size: 3327 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6493 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6493 BssSI C'ACGA_G Cuts at: 2128 4553 5565 2128 Size: 2425 1012 3056 Fragments arranged by size: 3056 2425 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3314 794 Size: 2520 3973 BstBI TT'CG_AA Cuts at: 3494 5297 3494 Size: 1803 4690 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6493 BstXI CCAn_nnnn'nTGG Cuts at: 2638 2638 Size: 6493 BstYI r'GATC_y Cuts at: 1972 2215 3661 3823 4190 4575 4592 6044 6130 Size: 243 1446 162 367 385 17 1452 86 Cuts at: 6130 6142 6488 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1446 385 367 346 243 162 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3061 3285 3657 513 Size: 552 939 1057 224 372 3349 Fragments arranged by size: 3349 1057 939 552 372 224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5754 Fragments arranged by size: 5754 683 56 BtsI GCAGTG_nn' Cuts at: 2506 2631 3696 4009 4803 4823 2506 Size: 125 1065 313 794 20 4176 Fragments arranged by size: 4176 1065 794 313 125 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 3797 3797 Size: 6493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2243 5500 809 Size: 507 927 3257 1802 Fragments arranged by size: 3257 1802 927 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2137 2569 Size: 54 1425 50 275 18 255 52 432 Cuts at: 2569 2643 3768 3940 4829 6458 8 Size: 74 1125 172 889 1629 43 Fragments arranged by size: 1629 1425 1125 889 432 275 255 172 74 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2569 3940 1537 Size: 275 757 1371 4090 Fragments arranged by size: 4090 1371 757 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4423 1353 Size: 3070 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3401 5080 5454 5600 387 Size: 1064 138 435 1377 1679 374 146 1280 Fragments arranged by size: 1679 1377 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2133 2133 Size: 6493 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2829 3239 3550 4556 5153 5940 6208 Size: 393 1197 410 311 1006 597 787 268 Cuts at: 6208 1239 Size: 1524 Fragments arranged by size: 1524 1197 1006 787 597 410 393 311 268 EcoO109I rG'GnC_Cy Cuts at: 3406 3406 Size: 6493 EcoRI G'AATT_C Cuts at: 3802 4180 3802 Size: 378 6115 FspI TGC'GCA Cuts at: 3646 4999 3646 Size: 1353 5140 HaeII r_GCGC'y Cuts at: 1678 2533 3001 5640 1678 Size: 855 468 2639 2531 Fragments arranged by size: 2639 2531 855 468 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 2232 2264 2372 Size: 32 135 32 587 32 41 32 108 Cuts at: 2372 2404 2824 2856 2860 2892 3266 3298 5134 Size: 32 420 32 4 32 374 32 1836 Cuts at: 5134 5166 5208 5240 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1836 587 420 374 135 108 42 41 32 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 2815 3837 4083 669 Size: 988 281 877 1022 246 3079 Fragments arranged by size: 3079 1022 988 877 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6493 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KpnI G_GTAC'C Cuts at: 3818 3818 Size: 6493 MfeI C'AATT_G Cuts at: 4092 4092 Size: 6493 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5607 5791 1305 Size: 4302 184 2007 Fragments arranged by size: 4302 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2087 2645 10 Size: 54 1425 343 255 558 3858 Fragments arranged by size: 3858 1425 558 343 255 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2487 2558 2636 3599 4451 4810 Size: 648 1011 310 71 78 963 852 359 Cuts at: 4810 4969 518 Size: 159 2042 Fragments arranged by size: 2042 1011 963 852 648 359 310 159 78 71 MspA1I CmG'CkG Cuts at: 1606 3479 3909 4586 5734 5979 1606 Size: 1873 430 677 1148 245 2120 Fragments arranged by size: 2120 1873 1148 677 430 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3657 513 Size: 552 939 1653 3349 Fragments arranged by size: 3349 1653 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6493 NheI G'CTAG_C Cuts at: 1052 3083 1052 Size: 2031 4462 NotI GC'GGCC_GC Cuts at: 3940 3940 Size: 6493 NsiI A_TGCA'T Cuts at: 3782 3782 Size: 6493 NspI r_CATG'y Cuts at: 1132 1229 2124 2316 2702 2889 3296 3784 3851 Size: 97 895 192 386 187 407 488 67 Cuts at: 3851 5396 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 895 488 407 386 192 187 97 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6493 PciI A'CATG_T Cuts at: 5392 5392 Size: 6493 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6493 PfoI T'CCnGG_A Cuts at: 2034 2034 Size: 6493 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4562 4594 6075 6107 4562 Size: 32 1481 32 4948 Fragments arranged by size: 4948 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3406 3406 Size: 6493 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6493 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PspXI vC'TCGA_Gb Cuts at: 3108 3108 Size: 6493 PstI C_TGCA'G Cuts at: 830 3845 830 Size: 3015 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 3479 3479 Size: 6493 SacI G_AGCT'C Cuts at: 2135 2135 Size: 6493 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SbfI CC_TGCA'GG Cuts at: 3845 3845 Size: 6493 ScaI AGT'ACT Cuts at: 2339 4741 6453 2339 Size: 2402 1712 2379 Fragments arranged by size: 2402 2379 1712 SexAI A'CCwGG_T Cuts at: 2951 2951 Size: 6493 SfcI C'TryA_G Cuts at: 826 1045 2434 3841 4976 5657 5848 826 Size: 219 1389 1407 1135 681 191 1471 Fragments arranged by size: 1471 1407 1389 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 3820 1872 Size: 1948 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3108 4597 5498 5760 820 Size: 197 926 1165 1489 901 262 1553 Fragments arranged by size: 1553 1489 1165 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6493 SphI G_CATG'C Cuts at: 1132 2702 2889 3296 3851 1132 Size: 1570 187 407 555 3774 Fragments arranged by size: 3774 1570 555 407 187 SspI AAT'ATT Cuts at: 5 52 4417 5 Size: 47 4365 2081 Fragments arranged by size: 4365 2081 47 StuI AGG'CCT Cuts at: 1517 3138 1517 Size: 1621 4872 StyI C'CwwG_G Cuts at: 513 1065 2004 3318 3402 3657 3932 513 Size: 552 939 1314 84 255 275 3074 Fragments arranged by size: 3074 1314 939 552 275 255 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2253 2535 4520 4537 4690 4875 5320 Size: 1255 673 282 1985 17 153 185 445 Cuts at: 5320 325 Size: 1498 Fragments arranged by size: 1985 1498 1255 673 445 282 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2337 4739 6451 Size: 274 80 33 51 1443 360 2402 1712 Cuts at: 6451 96 Size: 138 Fragments arranged by size: 2402 1712 1443 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2573 2605 3444 3476 6453 6485 2573 Size: 32 839 32 2977 32 2581 Fragments arranged by size: 2977 2581 839 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3431 1316 Size: 2115 4378 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 3541 2638 Size: 903 5590 XhoI C'TCGA_G Cuts at: 1943 3108 1943 Size: 1165 5328 XmaI C'CCGG_G Cuts at: 1870 3818 1870 Size: 1948 4545 XmnI GAAnn'nnTTC Cuts at: 2104 4622 2104 Size: 2518 3975 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI