Restriction map for
pFN21AE0303
(Linear) (Six-base) MAPSORT of: KIEE0303.seq Check: 3854 from: 1 to: 1788
KIEE0303.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 54 1788
Size: 54 1734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1788
Size: 824 964
AfeI AGC'GCT
Cuts at: 0 994 1788
Size: 994 794
AleI CACnn'nnGTG
Cuts at: 0 482 553 631 1788
Size: 482 71 78 1157
Fragments arranged by size:
1157 482 78 71
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1310 1788
Size: 1310 478
AlwNI CAG_nnn'CTG
Cuts at: 0 1477 1788
Size: 1477 311
ApaLI G'TGCA_C
Cuts at: 0 1248 1788
Size: 1248 540
AseI AT'TA_AT
Cuts at: 0 739 1788
Size: 739 1049
AvaI C'yCGr_G
Cuts at: 0 1103 1788
Size: 1103 685
BamHI G'GATC_C
Cuts at: 0 1656 1788
Size: 1656 132
BanI G'GyrC_C
Cuts at: 0 55 661 1788
Size: 55 606 1127
Fragments arranged by size:
1127 606 55
BanII G_rGCy'C
Cuts at: 0 130 1788
Size: 130 1658
BbvCI CC'TCA_GC
Cuts at: 0 11 1788
Size: 11 1777
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 318 352 1788
Size: 318 34 1436
Fragments arranged by size:
1436 318 34
BclI T'GATC_A
Cuts at: 0 1113 1788
Size: 1113 675
BfrBI ATG'CAT
Cuts at: 0 1775 1788
Size: 1775 13
BlpI GC'TnA_GC
Cuts at: 0 899 1788
Size: 899 889
Bme1580I G_kGCm'C
Cuts at: 0 58 664 1252 1700 1788
Size: 58 606 588 448 88
Fragments arranged by size:
606 588 448 88 58
BmgBI CAC'GTC
Cuts at: 0 573 1788
Size: 573 1215
BmrI ACTGGGnnnn_n'
Cuts at: 0 307 1788
Size: 307 1481
BmtI G_CTAG'C
Cuts at: 0 1082 1788
Size: 1082 706
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1055 1788
Size: 1055 733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1545 1788
Size: 1234 311 243
Fragments arranged by size:
1234 311 243
Bpu10I CC'TnA_GC
Cuts at: 0 11 989 1467 1788
Size: 11 978 478 321
Fragments arranged by size:
978 478 321 11
BsaWI w'CCGG_w
Cuts at: 0 222 1121 1788
Size: 222 899 667
Fragments arranged by size:
899 667 222
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 568 598 1788
Size: 568 30 1190
Fragments arranged by size:
1190 568 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 1788
Size: 49 1739
BseYI C'CCAG_C
Cuts at: 0 903 1788
Size: 903 885
BsiEI CG_ry'CG
Cuts at: 0 234 567 1788
Size: 234 333 1221
Fragments arranged by size:
1221 333 234
BsiHKAI G_wGCw'C
Cuts at: 0 130 503 1252 1788
Size: 130 373 749 536
Fragments arranged by size:
749 536 373 130
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1281 1563 1653 1788
Size: 1281 282 90 135
Fragments arranged by size:
1281 282 135 90
Bsp1286I G_dGCh'C
Cuts at: 0 58 130 503 664 1252 1700 1788
Size: 58 72 373 161 588 448 88
Fragments arranged by size:
588 448 373 161 88 72 58
BspEI T'CCGG_A
Cuts at: 0 222 1121 1788
Size: 222 899 667
Fragments arranged by size:
899 667 222
BspHI T'CATG_A
Cuts at: 0 191 368 1788
Size: 191 177 1420
Fragments arranged by size:
1420 191 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 54 1788
Size: 54 1734
BsrDI GCAATG_nn'
Cuts at: 0 416 487 1067 1788
Size: 416 71 580 721
Fragments arranged by size:
721 580 416 71
BssSI C'ACGA_G
Cuts at: 0 123 1788
Size: 123 1665
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1309 1788
Size: 1309 479
BstBI TT'CG_AA
Cuts at: 0 1489 1788
Size: 1489 299
BstXI CCAn_nnnn'nTGG
Cuts at: 0 633 1788
Size: 633 1155
BstYI r'GATC_y
Cuts at: 0 210 1656 1788
Size: 210 1446 132
Fragments arranged by size:
1446 210 132
BtgI C'CryG_G
Cuts at: 0 1056 1280 1652 1788
Size: 1056 224 372 136
Fragments arranged by size:
1056 372 224 136
BtsI GCAGTG_nn'
Cuts at: 0 501 626 1691 1788
Size: 501 125 1065 97
Fragments arranged by size:
1065 501 125 97
DrdI GACnn_nn'nnGTC
Cuts at: 0 238 1788
Size: 238 1550
EaeI y'GGCC_r
Cuts at: 0 80 132 564 638 1763 1788
Size: 80 52 432 74 1125 25
Fragments arranged by size:
1125 432 80 74 52 25
EagI C'GGCC_G
Cuts at: 0 564 1788
Size: 564 1224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1396 1788
Size: 19 1377 392
Fragments arranged by size:
1377 392 19
EcoICRI GAG'CTC
Cuts at: 0 128 1788
Size: 128 1660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1234 1545 1788
Size: 824 410 311 243
Fragments arranged by size:
824 410 311 243
EcoO109I rG'GnC_Cy
Cuts at: 0 1401 1788
Size: 1401 387
FspI TGC'GCA
Cuts at: 0 1641 1788
Size: 1641 147
HaeII r_GCGC'y
Cuts at: 0 528 996 1788
Size: 528 468 792
Fragments arranged by size:
792 528 468
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 227 259 367 399 819 851
Size: 154 32 41 32 108 32 420 32
Cuts at: 851 855 887 1261 1293 1788
Size: 4 32 374 32 495
Fragments arranged by size:
495 420 374 154 108 41 32 32
32 32 32 32 4
HincII GTy'rAC
Cuts at: 0 810 1788
Size: 810 978
MscI TGG'CCA
Cuts at: 0 82 640 1788
Size: 82 558 1148
Fragments arranged by size:
1148 558 82
MslI CAynn'nnrTG
Cuts at: 0 172 482 553 631 1594 1788
Size: 172 310 71 78 963 194
Fragments arranged by size:
963 310 194 172 78 71
MspA1I CmG'CkG
Cuts at: 0 1474 1788
Size: 1474 314
NcoI C'CATG_G
Cuts at: 0 1652 1788
Size: 1652 136
NheI G'CTAG_C
Cuts at: 0 1078 1788
Size: 1078 710
NsiI A_TGCA'T
Cuts at: 0 1777 1788
Size: 1777 11
NspI r_CATG'y
Cuts at: 0 119 311 697 884 1291 1779 1788
Size: 119 192 386 187 407 488 9
Fragments arranged by size:
488 407 386 192 187 119 9
PfoI T'CCnGG_A
Cuts at: 0 29 1788
Size: 29 1759
PpuMI rG'GwC_Cy
Cuts at: 0 1401 1788
Size: 1401 387
PspXI vC'TCGA_Gb
Cuts at: 0 1103 1788
Size: 1103 685
PvuII CAG'CTG
Cuts at: 0 1474 1788
Size: 1474 314
SacI G_AGCT'C
Cuts at: 0 130 1788
Size: 130 1658
ScaI AGT'ACT
Cuts at: 0 334 1788
Size: 334 1454
SexAI A'CCwGG_T
Cuts at: 0 946 1788
Size: 946 842
SfcI C'TryA_G
Cuts at: 0 429 1788
Size: 429 1359
SmlI C'TyrA_G
Cuts at: 0 1103 1788
Size: 1103 685
SphI G_CATG'C
Cuts at: 0 697 884 1291 1788
Size: 697 187 407 497
Fragments arranged by size:
697 497 407 187
StuI AGG'CCT
Cuts at: 0 1133 1788
Size: 1133 655
StyI C'CwwG_G
Cuts at: 0 1313 1397 1652 1788
Size: 1313 84 255 136
Fragments arranged by size:
1313 255 136 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 248 530 1788
Size: 248 282 1258
Fragments arranged by size:
1258 282 248
TatI w'GTAC_w
Cuts at: 0 332 1788
Size: 332 1456
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 568 600 1439 1471 1788
Size: 568 32 839 32 317
Fragments arranged by size:
839 568 317 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1426 1788
Size: 1426 362
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 633 1536 1788
Size: 633 903 252
Fragments arranged by size:
903 633 252
XhoI C'TCGA_G
Cuts at: 0 1103 1788
Size: 1103 685
XmnI GAAnn'nnTTC
Cuts at: 0 99 1788
Size: 99 1689
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApaLI AseI
AvaI BamHI BanI BanII BbvCI BcgI BclI BfrBI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BssSI BstAPI BstBI BstXI
BstYI BtgI BtsI DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoO109I FspI HaeII Hin4I HincII MscI MslI
MspA1I NcoI NheI NsiI NspI PfoI PpuMI PspXI
PvuII SacI ScaI SexAI SfcI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI ApaI ApoI AscI AsiSI AvrII BaeI BbeI
BbsI BciVI BglI BglII BpuEI BsaI BsaAI BsaBI
BsaHI BsgI BsiWI BsmI BsrBI BsrFI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EarI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NotI NruI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI XbaI XmaI ZraI