(Circular) (Six-base) MAPSORT of: pFN21AE0295.seq Check: 7322 from: 1 to: 5413 pFN21AE0295 5413 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3223 278 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2756 1937 Size: 819 4594 Acc65I G'GTAC_C Cuts at: 2734 2734 Size: 5413 AclI AA'CG_TT Cuts at: 1563 3540 3913 1563 Size: 1977 373 3063 Fragments arranged by size: 3063 1977 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3476 4860 5128 1632 Size: 1844 1384 268 1917 Fragments arranged by size: 1917 1844 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5216 AflIII A'CryG_T Cuts at: 1203 4312 5289 1203 Size: 3109 977 1327 Fragments arranged by size: 3109 1327 977 AgeI A'CCGG_T Cuts at: 4244 4244 Size: 5413 AhdI GACnn_n'nnGTC Cuts at: 4142 4142 Size: 5413 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5413 AlwNI CAG_nnn'CTG Cuts at: 3156 4728 3156 Size: 1572 3841 ApaI G_GGCC'C Cuts at: 2337 2497 2665 2337 Size: 160 168 5085 Fragments arranged by size: 5085 168 160 ApaLI G'TGCA_C Cuts at: 2585 3470 4626 2585 Size: 885 1156 3372 Fragments arranged by size: 3372 1156 885 ApoI r'AATT_y Cuts at: 1493 2722 2948 3100 5209 1493 Size: 1229 226 152 2109 1697 Fragments arranged by size: 2109 1697 1229 226 152 AseI AT'TA_AT Cuts at: 160 1032 3967 160 Size: 872 2935 1606 Fragments arranged by size: 2935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2078 2166 2421 2490 2496 2738 Size: 73 76 59 88 255 69 6 242 Cuts at: 2738 1870 Size: 4545 Fragments arranged by size: 4545 255 242 88 76 73 69 59 6 BamHI G'GATC_C Cuts at: 2743 3110 2743 Size: 367 5046 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2150 2288 2400 2408 Size: 325 217 627 227 136 138 112 8 Cuts at: 2408 2486 2734 4189 618 Size: 78 248 1455 1842 Fragments arranged by size: 1842 1455 627 325 248 227 217 138 136 112 78 8 BanII G_rGCy'C Cuts at: 1391 2064 2079 2337 2497 2665 2676 2732 1391 Size: 673 15 258 160 168 11 56 4072 Fragments arranged by size: 4072 673 258 168 160 56 15 11 BbeI G_GCGC'C Cuts at: 2292 2404 2292 Size: 112 5301 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2029 928 Size: 971 130 4312 Fragments arranged by size: 4312 971 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2132 2166 3604 3638 1903 Size: 34 195 34 1438 34 3678 Fragments arranged by size: 3678 1438 195 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3305 4515 3305 Size: 1210 4203 BclI T'GATC_A Cuts at: 1548 1548 Size: 5413 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4024 136 Size: 107 122 71 631 605 2352 1525 Fragments arranged by size: 2352 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5408 5408 Size: 5413 BlpI GC'TnA_GC Cuts at: 2830 2830 Size: 5413 Bme1580I G_kGCm'C Cuts at: 1790 2155 2201 2337 2413 2497 2589 2665 3474 Size: 365 46 136 76 84 92 76 809 Cuts at: 3474 4630 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 809 365 136 92 84 76 76 46 BmgBI CAC'GTC Cuts at: 1318 2584 1318 Size: 1266 4147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2061 2067 3144 Size: 416 53 83 399 24 644 6 1077 Cuts at: 3144 4102 442 Size: 958 1753 Fragments arranged by size: 1753 1077 958 644 416 399 83 53 24 6 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2726 4073 1239 Size: 905 582 1347 2579 Fragments arranged by size: 2579 1347 905 582 Bpu10I CC'TnA_GC Cuts at: 2243 2318 2243 Size: 75 5338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3538 4403 4701 3538 Size: 865 298 4250 Fragments arranged by size: 4250 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2021 4076 882 Size: 617 522 2055 2219 Fragments arranged by size: 2219 2055 617 522 BsaAI yAC'GTr Cuts at: 493 1204 3204 493 Size: 711 2000 2702 Fragments arranged by size: 2702 2000 711 BsaBI GATnn'nnATC Cuts at: 5407 5407 Size: 5413 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2289 2401 3220 3602 Size: 53 83 186 936 756 112 819 382 Cuts at: 3602 4237 275 Size: 635 1451 Fragments arranged by size: 1451 936 819 756 635 382 186 112 83 53 BsaWI w'CCGG_w Cuts at: 3846 4244 4518 4665 5076 3846 Size: 398 274 147 411 4183 Fragments arranged by size: 4183 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2102 2132 1178 Size: 30 165 30 699 30 4459 Fragments arranged by size: 4459 699 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2107 1186 Size: 921 4492 BseYI C'CCAG_C Cuts at: 2699 4616 5112 2699 Size: 1917 496 3000 Fragments arranged by size: 3000 1917 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2594 1163 Size: 573 858 3982 Fragments arranged by size: 3982 858 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2863 3624 4652 1540 Size: 275 186 862 761 1028 2301 Fragments arranged by size: 2301 1028 862 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 2589 3474 3559 4630 2139 Size: 450 885 85 1071 2922 Fragments arranged by size: 2922 1071 885 450 85 BsmI GAATG_Cn' Cuts at: 2924 3017 2924 Size: 93 5320 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2079 2139 2155 2201 2337 2413 Size: 399 274 15 60 16 46 136 76 Cuts at: 2413 2497 2589 2665 2676 2732 3474 3559 4630 Size: 84 92 76 11 56 742 85 1071 Cuts at: 4630 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 274 136 92 85 84 76 76 60 56 46 16 15 11 BspEI T'CCGG_A Cuts at: 5076 5076 Size: 5413 BspHI T'CATG_A Cuts at: 3302 5032 3302 Size: 1730 3683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2768 844 Size: 1924 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2115 3300 1394 Size: 429 292 1185 3507 Fragments arranged by size: 3507 1185 429 292 BsrDI GCAATG_nn' Cuts at: 65 1248 3908 4082 65 Size: 1183 2660 174 1396 Fragments arranged by size: 2660 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2237 2449 4057 4244 1700 Size: 110 427 212 1608 187 2869 Fragments arranged by size: 2869 1608 427 212 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5413 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5413 BssSI C'ACGA_G Cuts at: 3473 4485 3473 Size: 1012 4401 BstAPI GCAn_nnn'nTGC Cuts at: 794 2593 794 Size: 1799 3614 BstBI TT'CG_AA Cuts at: 4217 4217 Size: 5413 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5413 BstYI r'GATC_y Cuts at: 1972 2743 3110 3495 3512 4964 5050 5062 5408 Size: 771 367 385 17 1452 86 12 346 Cuts at: 5408 1972 Size: 1977 Fragments arranged by size: 1977 1452 771 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2292 2482 2653 513 Size: 552 1227 190 171 3273 Fragments arranged by size: 3273 1227 552 190 171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2278 487 Size: 683 56 1052 3622 Fragments arranged by size: 3622 1052 683 56 BtsI GCAGTG_nn' Cuts at: 2214 2254 2929 3723 3743 2214 Size: 40 675 794 20 3884 Fragments arranged by size: 3884 794 675 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5413 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5378 DraI TTT'AAA Cuts at: 2717 2717 Size: 5413 DraIII CAC_nnn'GTG Cuts at: 2190 2190 Size: 5413 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4420 809 Size: 507 3104 1802 Fragments arranged by size: 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2860 3749 5378 Size: 54 1425 50 275 18 1030 889 1629 Cuts at: 5378 8 Size: 43 Fragments arranged by size: 1629 1425 1030 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2860 1537 Size: 275 1048 4090 Fragments arranged by size: 4090 1048 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2534 3343 1353 Size: 1181 809 3423 Fragments arranged by size: 3423 1181 809 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4000 4374 4520 387 Size: 1064 138 2411 374 146 1280 Fragments arranged by size: 2411 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2726 3476 4073 4860 5128 1239 Size: 393 512 582 750 597 787 268 1524 Fragments arranged by size: 1524 787 750 597 582 512 393 268 EcoO109I rG'GnC_Cy Cuts at: 2170 2254 2333 2461 2170 Size: 84 79 128 5122 Fragments arranged by size: 5122 128 84 79 EcoRI G'AATT_C Cuts at: 2722 3100 2722 Size: 378 5035 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2296 2296 Size: 5413 FspI TGC'GCA Cuts at: 3919 3919 Size: 5413 HaeII r_GCGC'y Cuts at: 1678 2209 2292 2404 4560 1678 Size: 531 83 112 2156 2531 Fragments arranged by size: 2531 2156 531 112 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4054 4086 4128 4160 1373 Size: 32 135 32 2482 32 42 32 2626 Fragments arranged by size: 2626 2482 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2757 3003 669 Size: 988 281 819 246 3079 Fragments arranged by size: 3079 988 819 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5413 HpaI GTT'AAC Cuts at: 3003 3003 Size: 5413 KasI G'GCGC_C Cuts at: 2288 2400 2288 Size: 112 5301 KpnI G_GTAC'C Cuts at: 2738 2738 Size: 5413 MfeI C'AATT_G Cuts at: 3012 3012 Size: 5413 MluI A'CGCG_T Cuts at: 5289 5289 Size: 5413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4527 4711 1305 Size: 3222 184 2007 Fragments arranged by size: 3222 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3591 Fragments arranged by size: 3591 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3371 3730 3889 518 Size: 648 2205 359 159 2042 Fragments arranged by size: 2205 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2829 3506 4654 4899 1606 Size: 1223 677 1148 245 2120 Fragments arranged by size: 2120 1223 1148 677 245 NaeI GCC'GGC Cuts at: 2451 2451 Size: 5413 NarI GG'CG_CC Cuts at: 2289 2401 2289 Size: 112 5301 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4861 NdeI CA'TA_TG Cuts at: 387 387 Size: 5413 NgoMIV G'CCGG_C Cuts at: 2449 2449 Size: 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5413 NotI GC'GGCC_GC Cuts at: 2860 2860 Size: 5413 NspI r_CATG'y Cuts at: 1132 1229 2197 2771 4316 1132 Size: 97 968 574 1545 2229 Fragments arranged by size: 2229 1545 968 574 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5413 PciI A'CATG_T Cuts at: 4312 4312 Size: 5413 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5413 PfoI T'CCnGG_A Cuts at: 2040 2172 2040 Size: 132 5281 PmeI GTTT'AAAC Cuts at: 2717 2717 Size: 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4995 5027 3482 Size: 32 1481 32 3868 Fragments arranged by size: 3868 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2170 2170 Size: 5413 PshAI GACnn'nnGTC Cuts at: 1613 2033 1613 Size: 420 4993 PsiI TTA'TAA Cuts at: 2983 2983 Size: 5413 PspOMI G'GGCC_C Cuts at: 2333 2493 2661 2333 Size: 160 168 5085 Fragments arranged by size: 5085 168 160 PstI C_TGCA'G Cuts at: 830 2310 2765 830 Size: 1480 455 3478 Fragments arranged by size: 3478 1480 455 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5413 SalI G'TCGA_C Cuts at: 1936 2755 1936 Size: 819 4594 SbfI CC_TGCA'GG Cuts at: 2765 2765 Size: 5413 ScaI AGT'ACT Cuts at: 3661 5373 3661 Size: 1712 3701 SfcI C'TryA_G Cuts at: 826 1045 2306 2761 3896 4577 4768 826 Size: 219 1261 455 1135 681 191 1471 Fragments arranged by size: 1471 1261 1135 681 455 219 191 SfoI GGC'GCC Cuts at: 2290 2402 2290 Size: 112 5301 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5413 SmaI CCC'GGG Cuts at: 1872 2021 2168 2423 2492 2498 2740 1872 Size: 149 147 255 69 6 242 4545 Fragments arranged by size: 4545 255 242 149 147 69 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 3517 4418 4680 820 Size: 197 926 1574 901 262 1553 Fragments arranged by size: 1574 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3204 493 Size: 2711 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5413 SphI G_CATG'C Cuts at: 1132 2771 1132 Size: 1639 3774 SspI AAT'ATT Cuts at: 5 52 3337 5 Size: 47 3285 2081 Fragments arranged by size: 3285 2081 47 StuI AGG'CCT Cuts at: 1517 2312 1517 Size: 795 4618 StyI C'CwwG_G Cuts at: 513 1065 2220 2852 513 Size: 552 1155 632 3074 Fragments arranged by size: 3074 1155 632 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2396 2646 3440 3457 3610 3795 4240 Size: 1255 816 250 794 17 153 185 445 Cuts at: 4240 325 Size: 1498 Fragments arranged by size: 1498 1255 816 794 445 250 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2679 3659 5371 Size: 274 80 33 51 1443 702 980 1712 Cuts at: 5371 96 Size: 138 Fragments arranged by size: 1712 1443 980 702 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2102 2134 5373 5405 2102 Size: 32 3239 32 2110 Fragments arranged by size: 3239 2110 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2429 1316 Size: 1113 4300 XbaI T'CTAG_A Cuts at: 2749 2749 Size: 5413 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2344 2344 Size: 5413 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5413 XmaI C'CCGG_G Cuts at: 1870 2019 2166 2421 2490 2496 2738 1870 Size: 149 147 255 69 6 242 4545 Fragments arranged by size: 4545 255 242 149 147 69 6 XmnI GAAnn'nnTTC Cuts at: 3542 3542 Size: 5413 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3221 276 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI