Restriction map for pFN21AE0295
 (Linear) (Six-base) MAPSORT of: KIEE0295.seq  Check: 123  from: 1  to: 708



KIEE0295.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

ApaI G_GGCC'C

Cuts at:      0     332     492     660     708 
   Size:        332     160     168      48

  Fragments arranged by size: 

                 332     168     160      48
 
ApaLI G'TGCA_C

Cuts at:      0     580     708 
   Size:        580     128
 
AvaI C'yCGr_G

Cuts at:      0      14      73     161     416     485     491     708 
   Size:         14      59      88     255      69       6     217

  Fragments arranged by size: 

                 255     217      88      69      59      14       6
 
BanI G'GyrC_C

Cuts at:      0       9     145     283     395     403     481     708 
   Size:          9     136     138     112       8      78     227

  Fragments arranged by size: 

                 227     138     136     112      78       9       8
 
BanII G_rGCy'C

Cuts at:      0      59      74     332     492     660     671     708 
   Size:         59      15     258     160     168      11      37

  Fragments arranged by size: 

                 258     168     160      59      37      15      11
 
BbeI G_GCGC'C

Cuts at:      0     287     399     708 
   Size:        287     112     309

  Fragments arranged by size: 

                 309     287     112
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      24     708 
   Size:         24     684
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     127     161     708 
   Size:        127      34     547

  Fragments arranged by size: 

                 547     127      34
 
Bme1580I G_kGCm'C

Cuts at:      0     150     196     332     408     492     584     660     708 
   Size:        150      46     136      76      84      92      76      48

  Fragments arranged by size: 

                 150     136      92      84      76      76      48      46
            
 
BmgBI CAC'GTC

Cuts at:      0     579     708 
   Size:        579     129
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      56      62     708 
   Size:         56       6     646

  Fragments arranged by size: 

                 646      56       6
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     708 
   Size:        139     569
 
Bpu10I CC'TnA_GC

Cuts at:      0     238     313     708 
   Size:        238      75     395

  Fragments arranged by size: 

                 395     238      75
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      16     708 
   Size:         16     692
 
BsaHI Gr'CG_yC

Cuts at:      0     284     396     708 
   Size:        284     112     312

  Fragments arranged by size: 

                 312     284     112
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      97     127     708 
   Size:         97      30     581

  Fragments arranged by size: 

                 581      97      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     102     708 
   Size:        102     606
 
BseYI C'CCAG_C

Cuts at:      0     694     708 
   Size:        694      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     589     708 
   Size:        589     119
 
BsiHKAI G_wGCw'C

Cuts at:      0     134     584     708 
   Size:        134     450     124

  Fragments arranged by size: 

                 450     134     124
 
Bsp1286I G_dGCh'C

Cuts at:      0      59      74     134     150     196     332     408     492 
   Size:         59      15      60      16      46     136      76      84

Cuts at:    492     584     660     671     708 
   Size:         92      76      11      37

  Fragments arranged by size: 

                 136      92      84      76      76      60      59      46
                  37      16      15      11
 
BsrBI CCG'CTC

Cuts at:      0     110     708 
   Size:        110     598
 
BsrFI r'CCGG_y

Cuts at:      0     232     444     708 
   Size:        232     212     264

  Fragments arranged by size: 

                 264     232     212
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     588     708 
   Size:        588     120
 
BtgI C'CryG_G

Cuts at:      0     287     477     648     708 
   Size:        287     190     171      60

  Fragments arranged by size: 

                 287     190     171      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     273     708 
   Size:        273     435
 
BtsI GCAGTG_nn'

Cuts at:      0     209     249     708 
   Size:        209      40     459

  Fragments arranged by size: 

                 459     209      40
 
DraIII CAC_nnn'GTG

Cuts at:      0     185     708 
   Size:        185     523
 
EarI CTCTTCn'nnn_

Cuts at:      0     529     708 
   Size:        529     179
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     708 
   Size:        139     569
 
EcoO109I rG'GnC_Cy

Cuts at:      0     165     249     328     456     708 
   Size:        165      84      79     128     252

  Fragments arranged by size: 

                 252     165     128      84      79
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     291     708 
   Size:        291     417
 
HaeII r_GCGC'y

Cuts at:      0     204     287     399     708 
   Size:        204      83     112     309

  Fragments arranged by size: 

                 309     204     112      83
 
KasI G'GCGC_C

Cuts at:      0     283     395     708 
   Size:        283     112     313

  Fragments arranged by size: 

                 313     283     112
 
NaeI GCC'GGC

Cuts at:      0     446     708 
   Size:        446     262
 
NarI GG'CG_CC

Cuts at:      0     284     396     708 
   Size:        284     112     312

  Fragments arranged by size: 

                 312     284     112
 
NgoMIV G'CCGG_C

Cuts at:      0     444     708 
   Size:        444     264
 
NspI r_CATG'y

Cuts at:      0     192     708 
   Size:        192     516
 
PfoI T'CCnGG_A

Cuts at:      0      35     167     708 
   Size:         35     132     541

  Fragments arranged by size: 

                 541     132      35
 
PpuMI rG'GwC_Cy

Cuts at:      0     165     708 
   Size:        165     543
 
PshAI GACnn'nnGTC

Cuts at:      0      28     708 
   Size:         28     680
 
PspOMI G'GGCC_C

Cuts at:      0     328     488     656     708 
   Size:        328     160     168      52

  Fragments arranged by size: 

                 328     168     160      52
 
PstI C_TGCA'G

Cuts at:      0     305     708 
   Size:        305     403
 
SfcI C'TryA_G

Cuts at:      0     301     708 
   Size:        301     407
 
SfoI GGC'GCC

Cuts at:      0     285     397     708 
   Size:        285     112     311

  Fragments arranged by size: 

                 311     285     112
 
SmaI CCC'GGG

Cuts at:      0      16     163     418     487     493     708 
   Size:         16     147     255      69       6     215

  Fragments arranged by size: 

                 255     215     147      69      16       6
 
StuI AGG'CCT

Cuts at:      0     307     708 
   Size:        307     401
 
StyI C'CwwG_G

Cuts at:      0     215     708 
   Size:        215     493
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     391     641     708 
   Size:        391     250      67

  Fragments arranged by size: 

                 391     250      67
 
TatI w'GTAC_w

Cuts at:      0     674     708 
   Size:        674      34
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      97     129     708 
   Size:         97      32     579

  Fragments arranged by size: 

                 579      97      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     424     708 
   Size:        424     284
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     339     708 
   Size:        339     369
 
XmaI C'CCGG_G

Cuts at:      0      14     161     416     485     491     708 
   Size:         14     147     255      69       6     217

  Fragments arranged by size: 

                 255     217     147      69      14       6
 

 Enzymes that do cut:

     ApaI    ApaLI     AvaI     BanI    BanII     BbeI     BbsI     BcgI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrBI    BsrFI   BstAPI
     BtgI    BtgZI     BtsI   DraIII     EarI  Eco57MI EcoO109I     FalI
    HaeII     KasI     NaeI     NarI   NgoMIV     NspI     PfoI    PpuMI
    PshAI   PspOMI     PstI     SfcI     SfoI     SmaI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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