Restriction map for
pFN21AE0294
(Linear) (Six-base) MAPSORT of: KIEE0294.seq Check: 9778 from: 1 to: 942
KIEE0294.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 263 942
Size: 263 679
AccI GT'mk_AC
Cuts at: 0 866 942
Size: 866 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 700 942
Size: 138 562 242
Fragments arranged by size:
562 242 138
AleI CACnn'nnGTG
Cuts at: 0 580 942
Size: 580 362
AlwNI CAG_nnn'CTG
Cuts at: 0 78 246 942
Size: 78 168 696
Fragments arranged by size:
696 168 78
BanI G'GyrC_C
Cuts at: 0 546 627 942
Size: 546 81 315
Fragments arranged by size:
546 315 81
BanII G_rGCy'C
Cuts at: 0 71 942
Size: 71 871
BbvCI CC'TCA_GC
Cuts at: 0 239 607 690 695 942
Size: 239 368 83 5 247
Fragments arranged by size:
368 247 239 83 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 55 942
Size: 39 16 887
Fragments arranged by size:
887 39 16
Bpu10I CC'TnA_GC
Cuts at: 0 239 607 690 695 942
Size: 239 368 83 5 247
Fragments arranged by size:
368 247 239 83 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 942
Size: 331 611
BseYI C'CCAG_C
Cuts at: 0 299 420 942
Size: 299 121 522
Fragments arranged by size:
522 299 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 942
Size: 552 390
BsiHKAI G_wGCw'C
Cuts at: 0 71 942
Size: 71 871
BsmI GAATG_Cn'
Cuts at: 0 923 942
Size: 923 19
Bsp1286I G_dGCh'C
Cuts at: 0 71 942
Size: 71 871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 263 942
Size: 263 679
BsrDI GCAATG_nn'
Cuts at: 0 344 942
Size: 344 598
BstAPI GCAn_nnn'nTGC
Cuts at: 0 335 942
Size: 335 607
BstYI r'GATC_y
Cuts at: 0 683 942
Size: 683 259
BtsI GCAGTG_nn'
Cuts at: 0 830 942
Size: 830 112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 101 136 942
Size: 101 35 806
Fragments arranged by size:
806 101 35
EaeI y'GGCC_r
Cuts at: 0 122 140 476 942
Size: 122 18 336 466
Fragments arranged by size:
466 336 122 18
EcoICRI GAG'CTC
Cuts at: 0 69 942
Size: 69 873
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 55 138 700 942
Size: 39 16 83 562 242
Fragments arranged by size:
562 242 83 39 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 942
Size: 726 216
HincII GTy'rAC
Cuts at: 0 525 669 942
Size: 525 144 273
Fragments arranged by size:
525 273 144
HpaI GTT'AAC
Cuts at: 0 525 669 942
Size: 525 144 273
Fragments arranged by size:
525 273 144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 942
Size: 407 535
MscI TGG'CCA
Cuts at: 0 124 478 942
Size: 124 354 464
Fragments arranged by size:
464 354 124
MslI CAynn'nnrTG
Cuts at: 0 43 175 349 580 942
Size: 43 132 174 231 362
Fragments arranged by size:
362 231 174 132 43
MspA1I CmG'CkG
Cuts at: 0 75 158 243 303 694 942
Size: 75 83 85 60 391 248
Fragments arranged by size:
391 248 85 83 75 60
NspI r_CATG'y
Cuts at: 0 501 942
Size: 501 441
PvuII CAG'CTG
Cuts at: 0 75 158 243 303 694 942
Size: 75 83 85 60 391 248
Fragments arranged by size:
391 248 85 83 75 60
SacI G_AGCT'C
Cuts at: 0 71 942
Size: 71 871
SfcI C'TryA_G
Cuts at: 0 867 942
Size: 867 75
StuI AGG'CCT
Cuts at: 0 709 942
Size: 709 233
StyI C'CwwG_G
Cuts at: 0 315 942
Size: 315 627
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 592 942
Size: 313 279 350
Fragments arranged by size:
350 313 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 23 55 908 940 942
Size: 23 32 853 32 2
Fragments arranged by size:
853 32 32 23 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 942
Size: 282 660
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI BanI BanII BbvCI
BpmI Bpu10I BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BstAPI BstYI BtsI CspCI EaeI EcoICRI
Eco57MI FalI HincII HpaI MmeI MscI MslI MspA1I
NspI PvuII SacI SfcI StuI StyI TaqII TstI
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI DraI DraIII DrdI EagI EarI
EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HindIII KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmaI XmnI ZraI