(Circular) (Six-base) MAPSORT of: pFN21AE0291.seq Check: 9552 from: 1 to: 5926 pFN21AE0291 5926 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2494 3269 1937 Size: 557 775 4594 Fragments arranged by size: 4594 775 557 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 2690 3989 5373 5641 1632 Size: 545 513 1299 1384 268 1917 Fragments arranged by size: 1917 1384 1299 545 513 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 2843 4825 5802 1203 Size: 1640 1982 977 1327 Fragments arranged by size: 1982 1640 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2418 1184 Size: 1234 4692 AlwNI CAG_nnn'CTG Cuts at: 3114 3669 5241 3114 Size: 555 1572 3799 Fragments arranged by size: 3799 1572 555 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 3235 3461 3613 5722 1493 Size: 1742 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2739 4480 160 Size: 872 1707 1741 1606 Fragments arranged by size: 1741 1707 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 3251 1870 Size: 73 1308 4545 Fragments arranged by size: 4545 1308 73 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2822 3247 4702 618 Size: 325 217 627 1035 425 1455 1842 Fragments arranged by size: 1842 1455 1035 627 425 325 217 BanII G_rGCy'C Cuts at: 1391 2165 2221 2262 2349 2538 2583 3245 1391 Size: 774 56 41 87 189 45 662 4072 Fragments arranged by size: 4072 774 662 189 87 56 45 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2984 928 Size: 971 1085 3870 Fragments arranged by size: 3870 1085 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4117 4151 1903 Size: 34 2180 34 3678 Fragments arranged by size: 3678 2180 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 2328 3139 1548 Size: 780 811 4335 Fragments arranged by size: 4335 811 780 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4537 136 Size: 107 122 71 631 605 2865 1525 Fragments arranged by size: 2865 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5921 5921 Size: 5926 BlpI GC'TnA_GC Cuts at: 2240 3343 2240 Size: 1103 4823 Bme1580I G_kGCm'C Cuts at: 1790 3987 5143 1790 Size: 2197 1156 2573 Fragments arranged by size: 2573 2197 1156 BmgBI CAC'GTC Cuts at: 1318 2844 1318 Size: 1526 4400 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2343 2439 3657 Size: 416 53 83 399 24 926 96 1218 Cuts at: 3657 4615 442 Size: 958 1753 Fragments arranged by size: 1753 1218 958 926 416 399 96 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2363 2748 4586 1239 Size: 830 294 385 1838 2579 Fragments arranged by size: 2579 1838 830 385 294 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4051 4916 5214 4051 Size: 865 298 4763 Fragments arranged by size: 4763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2546 4589 882 Size: 617 1047 2043 2219 Fragments arranged by size: 2219 2043 1047 617 BsaAI yAC'GTr Cuts at: 493 1204 3717 493 Size: 711 2513 2702 Fragments arranged by size: 2702 2513 711 BsaBI GATnn'nnATC Cuts at: 2330 5920 2330 Size: 3590 2336 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2837 3733 4115 4750 Size: 53 83 186 936 1304 896 382 635 Cuts at: 4750 275 Size: 1451 Fragments arranged by size: 1451 1304 936 896 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2575 4359 4757 5031 5178 5589 2575 Size: 1784 398 274 147 411 2912 Fragments arranged by size: 2912 1784 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5701 Fragments arranged by size: 5701 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3114 1186 Size: 1928 3998 BseYI C'CCAG_C Cuts at: 2582 5129 5625 2582 Size: 2547 496 2883 Fragments arranged by size: 2883 2547 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2167 2619 1163 Size: 573 431 452 4470 Fragments arranged by size: 4470 573 452 431 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3376 4137 5165 1540 Size: 275 186 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2027 2538 3987 4072 5143 2027 Size: 511 1449 85 1071 2810 Fragments arranged by size: 2810 1449 1071 511 85 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 BsmBI CGTCTCn'nnnn_ Cuts at: 2194 2194 Size: 5926 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2165 2221 2262 2349 2538 2583 Size: 399 237 138 56 41 87 189 45 Cuts at: 2583 3245 3987 4072 5143 1391 Size: 662 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 662 399 237 189 138 87 85 56 45 41 BspEI T'CCGG_A Cuts at: 2575 5589 2575 Size: 3014 2912 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3281 844 Size: 2437 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2638 2783 4421 4595 65 Size: 1183 1390 145 1638 174 1396 Fragments arranged by size: 1638 1396 1390 1183 174 145 BsrFI r'CCGG_y Cuts at: 1700 1810 4570 4757 1700 Size: 110 2760 187 2869 Fragments arranged by size: 2869 2760 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 2901 794 Size: 2107 3819 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstXI CCAn_nnnn'nTGG Cuts at: 2726 2726 Size: 5926 BstYI r'GATC_y Cuts at: 1972 2044 2399 3256 3623 4008 4025 5477 5563 Size: 72 355 857 367 385 17 1452 86 Cuts at: 5563 5575 5921 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 857 385 367 355 346 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2253 513 Size: 552 939 249 4186 Fragments arranged by size: 4186 939 552 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2092 487 Size: 683 56 866 4321 Fragments arranged by size: 4321 866 683 56 BtsI GCAGTG_nn' Cuts at: 2100 2607 3442 4236 4256 2100 Size: 507 835 794 20 3770 Fragments arranged by size: 3770 835 794 507 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DraIII CAC_nnn'GTG Cuts at: 2341 2341 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3189 3373 4262 Size: 54 1425 50 275 18 1359 184 889 Cuts at: 4262 5891 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1359 889 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2657 3856 1353 Size: 1304 1199 3423 Fragments arranged by size: 3423 1304 1199 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2536 2536 Size: 5926 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2177 2363 2690 2748 3989 4586 Size: 393 437 108 186 327 58 1241 597 Cuts at: 4586 5373 5641 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1241 787 597 437 393 327 268 186 108 58 EcoNI CCTnn'n_nnAGG Cuts at: 2290 2290 Size: 5926 EcoO109I rG'GnC_Cy Cuts at: 2135 2135 Size: 5926 EcoRI G'AATT_C Cuts at: 3235 3613 3235 Size: 378 5548 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2825 2825 Size: 5926 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 5073 1678 Size: 3395 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2361 2393 2698 2730 2833 Size: 32 135 32 789 32 305 32 103 Cuts at: 2833 2865 4567 4599 4641 4673 1373 Size: 32 1702 32 42 32 2626 Fragments arranged by size: 2626 1702 789 305 135 103 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2785 5040 5224 1305 Size: 1480 2255 184 2007 Fragments arranged by size: 2255 2007 1480 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3191 10 Size: 54 1425 343 1359 2745 Fragments arranged by size: 2745 1425 1359 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2234 3884 4243 4402 518 Size: 648 1068 1650 359 159 2042 Fragments arranged by size: 2042 1650 1068 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2066 3133 3342 4019 5167 5412 1606 Size: 460 1067 209 677 1148 245 2120 Fragments arranged by size: 2120 1148 1067 677 460 245 209 NcoI C'CATG_G Cuts at: 513 1065 2004 2253 513 Size: 552 939 249 4186 Fragments arranged by size: 4186 939 552 249 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 3284 4829 1132 Size: 97 2055 1545 2229 Fragments arranged by size: 2229 2055 1545 97 PasI CC'CwG_GG Cuts at: 1348 2194 1348 Size: 846 5080 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 2341 2425 1108 Size: 1233 84 4609 Fragments arranged by size: 4609 1233 84 PfoI T'CCnGG_A Cuts at: 2517 2517 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2303 2335 3995 4027 5508 5540 2303 Size: 32 1660 32 1481 32 2689 Fragments arranged by size: 2689 1660 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PstI C_TGCA'G Cuts at: 830 3278 830 Size: 2448 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 SacI G_AGCT'C Cuts at: 2538 2538 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SapI GCTCTTCn'nnn_ Cuts at: 2657 2657 Size: 5926 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 2923 3219 3274 4409 5090 5281 826 Size: 219 1878 296 55 1135 681 191 1471 Fragments arranged by size: 1878 1471 1135 681 296 219 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 3253 1872 Size: 1381 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4030 4931 5193 820 Size: 197 926 2087 901 262 1553 Fragments arranged by size: 2087 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 3284 1132 Size: 2152 3774 SspI AAT'ATT Cuts at: 5 52 2743 3850 5 Size: 47 2691 1107 2081 Fragments arranged by size: 2691 2081 1107 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 2004 2253 3365 513 Size: 552 939 249 1112 3074 Fragments arranged by size: 3074 1112 939 552 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2528 3166 3953 3970 4123 4308 4753 Size: 1255 948 638 787 17 153 185 445 Cuts at: 4753 325 Size: 1498 Fragments arranged by size: 1498 1255 948 787 638 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2846 4172 5884 Size: 274 80 33 51 1443 869 1326 1712 Cuts at: 5884 96 Size: 138 Fragments arranged by size: 1712 1443 1326 869 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5886 5918 5886 Size: 32 5894 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 3251 1870 Size: 1381 4545 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI