Restriction map for pFN21AE0291
 (Circular) (Six-base) MAPSORT of: pFN21AE0291.seq  Check: 9552  from: 1  to: 5926



pFN21AE0291  5926 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:47  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3736     278 
   Size:         53      83     186     936    2200    2468

  Fragments arranged by size: 

                2468    2200     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2494    3269    1937 
   Size:        557     775    4594

  Fragments arranged by size: 

                4594     775     557
 
Acc65I G'GTAC_C

Cuts at:   3247    3247 
   Size:       5926
 
AclI AA'CG_TT

Cuts at:   1563    4053    4426    1563 
   Size:       2490     373    3063

  Fragments arranged by size: 

                3063    2490     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2177    2690    3989    5373    5641    1632 
   Size:        545     513    1299    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1299     545     513     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5729
 
AflIII A'CryG_T

Cuts at:   1203    2843    4825    5802    1203 
   Size:       1640    1982     977    1327

  Fragments arranged by size: 

                1982    1640    1327     977
 
AgeI A'CCGG_T

Cuts at:   4757    4757 
   Size:       5926
 
AhdI GACnn_n'nnGTC

Cuts at:   4655    4655 
   Size:       5926
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5926
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2418    1184 
   Size:       1234    4692
 
AlwNI CAG_nnn'CTG

Cuts at:   3114    3669    5241    3114 
   Size:        555    1572    3799

  Fragments arranged by size: 

                3799    1572     555
 
ApaLI G'TGCA_C

Cuts at:   3983    5139    3983 
   Size:       1156    4770
 
ApoI r'AATT_y

Cuts at:   1493    3235    3461    3613    5722    1493 
   Size:       1742     226     152    2109    1697

  Fragments arranged by size: 

                2109    1742    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2739    4480     160 
   Size:        872    1707    1741    1606

  Fragments arranged by size: 

                1741    1707    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5926
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3251    1870 
   Size:         73    1308    4545

  Fragments arranged by size: 

                4545    1308      73
 
BamHI G'GATC_C

Cuts at:   3256    3623    3256 
   Size:        367    5559
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2822    3247    4702     618 
   Size:        325     217     627    1035     425    1455    1842

  Fragments arranged by size: 

                1842    1455    1035     627     425     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2165    2221    2262    2349    2538    2583    3245    1391 
   Size:        774      56      41      87     189      45     662    4072

  Fragments arranged by size: 

                4072     774     662     189      87      56      45      41
            
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2984     928 
   Size:        971    1085    3870

  Fragments arranged by size: 

                3870    1085     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4117    4151    1903 
   Size:         34    2180      34    3678

  Fragments arranged by size: 

                3678    2180      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3818    5028    3818 
   Size:       1210    4716
 
BclI T'GATC_A

Cuts at:   1548    2328    3139    1548 
   Size:        780     811    4335

  Fragments arranged by size: 

                4335     811     780
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4537     136 
   Size:        107     122      71     631     605    2865    1525

  Fragments arranged by size: 

                2865    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5921    5921 
   Size:       5926
 
BlpI GC'TnA_GC

Cuts at:   2240    3343    2240 
   Size:       1103    4823
 
Bme1580I G_kGCm'C

Cuts at:   1790    3987    5143    1790 
   Size:       2197    1156    2573

  Fragments arranged by size: 

                2573    2197    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2844    1318 
   Size:       1526    4400
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2343    2439    3657 
   Size:        416      53      83     399      24     926      96    1218

Cuts at:   3657    4615     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1218     958     926     416     399      96      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5926
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2069    2363    2748    4586    1239 
   Size:        830     294     385    1838    2579

  Fragments arranged by size: 

                2579    1838     830     385     294
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4051    4916    5214    4051 
   Size:        865     298    4763

  Fragments arranged by size: 

                4763     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2546    4589     882 
   Size:        617    1047    2043    2219

  Fragments arranged by size: 

                2219    2043    1047     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3717     493 
   Size:        711    2513    2702

  Fragments arranged by size: 

                2702    2513     711
 
BsaBI GATnn'nnATC

Cuts at:   2330    5920    2330 
   Size:       3590    2336
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2837    3733    4115    4750 
   Size:         53      83     186     936    1304     896     382     635

Cuts at:   4750     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1304     936     896     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2575    4359    4757    5031    5178    5589    2575 
   Size:       1784     398     274     147     411    2912

  Fragments arranged by size: 

                2912    1784     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5701

  Fragments arranged by size: 

                5701     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3114    1186 
   Size:       1928    3998
 
BseYI C'CCAG_C

Cuts at:   2582    5129    5625    2582 
   Size:       2547     496    2883

  Fragments arranged by size: 

                2883    2547     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2167    2619    1163 
   Size:        573     431     452    4470

  Fragments arranged by size: 

                4470     573     452     431
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3376    4137    5165    1540 
   Size:        275     186    1375     761    1028    2301

  Fragments arranged by size: 

                2301    1375    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2027    2538    3987    4072    5143    2027 
   Size:        511    1449      85    1071    2810

  Fragments arranged by size: 

                2810    1449    1071     511      85
 
BsmI GAATG_Cn'

Cuts at:   3437    3530    3437 
   Size:         93    5833
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2194    2194 
   Size:       5926
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2027    2165    2221    2262    2349    2538    2583 
   Size:        399     237     138      56      41      87     189      45

Cuts at:   2583    3245    3987    4072    5143    1391 
   Size:        662     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     662     399     237     189     138
                  87      85      56      45      41
 
BspEI T'CCGG_A

Cuts at:   2575    5589    2575 
   Size:       3014    2912
 
BspHI T'CATG_A

Cuts at:   3815    5545    3815 
   Size:       1730    4196
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3281     844 
   Size:       2437    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3813    1394 
   Size:        429    1990    3507

  Fragments arranged by size: 

                3507    1990     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2638    2783    4421    4595      65 
   Size:       1183    1390     145    1638     174    1396

  Fragments arranged by size: 

                1638    1396    1390    1183     174     145
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4570    4757    1700 
   Size:        110    2760     187    2869

  Fragments arranged by size: 

                2869    2760     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5926
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5926
 
BssSI C'ACGA_G

Cuts at:   3986    4998    3986 
   Size:       1012    4914
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2901     794 
   Size:       2107    3819
 
BstBI TT'CG_AA

Cuts at:   4730    4730 
   Size:       5926
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5926
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2726    2726 
   Size:       5926
 
BstYI r'GATC_y

Cuts at:   1972    2044    2399    3256    3623    4008    4025    5477    5563 
   Size:         72     355     857     367     385      17    1452      86

Cuts at:   5563    5575    5921    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     857     385     367     355     346      86
                  72      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2253     513 
   Size:        552     939     249    4186

  Fragments arranged by size: 

                4186     939     552     249
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2092     487 
   Size:        683      56     866    4321

  Fragments arranged by size: 

                4321     866     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2100    2607    3442    4236    4256    2100 
   Size:        507     835     794      20    3770

  Fragments arranged by size: 

                3770     835     794     507      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5926
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5891
 
DraI TTT'AAA

Cuts at:   3230    3230 
   Size:       5926
 
DraIII CAC_nnn'GTG

Cuts at:   2341    2341 
   Size:       5926
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4933     809 
   Size:        507    3617    1802

  Fragments arranged by size: 

                3617    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3189    3373    4262 
   Size:         54    1425      50     275      18    1359     184     889

Cuts at:   4262    5891       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1359     889     275     184      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3373    1537 
   Size:        275    1561    4090

  Fragments arranged by size: 

                4090    1561     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2657    3856    1353 
   Size:       1304    1199    3423

  Fragments arranged by size: 

                3423    1304    1199
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4513    4887    5033     387 
   Size:       1064     138    2924     374     146    1280

  Fragments arranged by size: 

                2924    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2536    2536 
   Size:       5926
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2069    2177    2363    2690    2748    3989    4586 
   Size:        393     437     108     186     327      58    1241     597

Cuts at:   4586    5373    5641    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524    1241     787     597     437     393     327     268
                 186     108      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2290    2290 
   Size:       5926
 
EcoO109I rG'GnC_Cy

Cuts at:   2135    2135 
   Size:       5926
 
EcoRI G'AATT_C

Cuts at:   3235    3613    3235 
   Size:        378    5548
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2825    2825 
   Size:       5926
 
FspI TGC'GCA

Cuts at:   4432    4432 
   Size:       5926
 
HaeII r_GCGC'y

Cuts at:   1678    5073    1678 
   Size:       3395    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2361    2393    2698    2730    2833 
   Size:         32     135      32     789      32     305      32     103

Cuts at:   2833    2865    4567    4599    4641    4673    1373 
   Size:         32    1702      32      42      32    2626

  Fragments arranged by size: 

                2626    1702     789     305     135     103      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3270    3516     669 
   Size:        988     281    1332     246    3079

  Fragments arranged by size: 

                3079    1332     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5926
 
HpaI GTT'AAC

Cuts at:   3516    3516 
   Size:       5926
 
KpnI G_GTAC'C

Cuts at:   3251    3251 
   Size:       5926
 
MfeI C'AATT_G

Cuts at:   3525    3525 
   Size:       5926
 
MluI A'CGCG_T

Cuts at:   5802    5802 
   Size:       5926
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2785    5040    5224    1305 
   Size:       1480    2255     184    2007

  Fragments arranged by size: 

                2255    2007    1480     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3191      10 
   Size:         54    1425     343    1359    2745

  Fragments arranged by size: 

                2745    1425    1359     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2234    3884    4243    4402     518 
   Size:        648    1068    1650     359     159    2042

  Fragments arranged by size: 

                2042    1650    1068     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2066    3133    3342    4019    5167    5412    1606 
   Size:        460    1067     209     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1067     677     460     245     209
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2253     513 
   Size:        552     939     249    4186

  Fragments arranged by size: 

                4186     939     552     249
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5926
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5926
 
NotI GC'GGCC_GC

Cuts at:   3373    3373 
   Size:       5926
 
NspI r_CATG'y

Cuts at:   1132    1229    3284    4829    1132 
   Size:         97    2055    1545    2229

  Fragments arranged by size: 

                2229    2055    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2194    1348 
   Size:        846    5080
 
PciI A'CATG_T

Cuts at:   4825    4825 
   Size:       5926
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2341    2425    1108 
   Size:       1233      84    4609

  Fragments arranged by size: 

                4609    1233      84
 
PfoI T'CCnGG_A

Cuts at:   2517    2517 
   Size:       5926
 
PmeI GTTT'AAAC

Cuts at:   3230    3230 
   Size:       5926
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2303    2335    3995    4027    5508    5540    2303 
   Size:         32    1660      32    1481      32    2689

  Fragments arranged by size: 

                2689    1660    1481      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5926
 
PsiI TTA'TAA

Cuts at:   3496    3496 
   Size:       5926
 
PstI C_TGCA'G

Cuts at:    830    3278     830 
   Size:       2448    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5926
 
SacI G_AGCT'C

Cuts at:   2538    2538 
   Size:       5926
 
SalI G'TCGA_C

Cuts at:   1936    3268    1936 
   Size:       1332    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2657    2657 
   Size:       5926
 
SbfI CC_TGCA'GG

Cuts at:   3278    3278 
   Size:       5926
 
ScaI AGT'ACT

Cuts at:   4174    5886    4174 
   Size:       1712    4214
 
SfcI C'TryA_G

Cuts at:    826    1045    2923    3219    3274    4409    5090    5281     826 
   Size:        219    1878     296      55    1135     681     191    1471

  Fragments arranged by size: 

                1878    1471    1135     681     296     219     191      55
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5926
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5926
 
SmaI CCC'GGG

Cuts at:   1872    3253    1872 
   Size:       1381    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4030    4931    5193     820 
   Size:        197     926    2087     901     262    1553

  Fragments arranged by size: 

                2087    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3717     493 
   Size:       3224    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5926
 
SphI G_CATG'C

Cuts at:   1132    3284    1132 
   Size:       2152    3774
 
SspI AAT'ATT

Cuts at:      5      52    2743    3850       5 
   Size:         47    2691    1107    2081

  Fragments arranged by size: 

                2691    2081    1107      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5926
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2253    3365     513 
   Size:        552     939     249    1112    3074

  Fragments arranged by size: 

                3074    1112     939     552     249
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2528    3166    3953    3970    4123    4308    4753 
   Size:       1255     948     638     787      17     153     185     445

Cuts at:   4753     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255     948     787     638     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2846    4172    5884 
   Size:        274      80      33      51    1443     869    1326    1712

Cuts at:   5884      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1326     869     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5886    5918    5886 
   Size:         32    5894
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5926
 
XbaI T'CTAG_A

Cuts at:   3262    3262 
   Size:       5926
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5926
 
XmaI C'CCGG_G

Cuts at:   1870    3251    1870 
   Size:       1381    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4055    4055 
   Size:       5926
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3734     276 
   Size:         53      83     186     936    2200    2468

  Fragments arranged by size: 

                2468    2200     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI  BstZ17I   Bsu36I    EcoRV
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII
    SacII    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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