Restriction map for
pFN21AE0289
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII AccI AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AspEI AspHI AspI
AvaI AvaII AviII AvrII BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII ItaI KasI Ksp632I KspI
MamI MluI MvnI MwoI NaeI
NarI NciI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TseI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 757 GGTACC G^GTACC
AccIII 607 TCCGGA T^CCGGA
AciI 363 CCGC CCGC(-3/-1)
AocI 675 CCTAAGG CC^T[ACGT]AGG
AseI 468 ATTAAT AT^TAAT
AsnI 468 ATTAAT AT^TAAT
Asp700I 921 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 757 GGTACC G^GTACC
BamHI 680 GGATCC G^GATCC
BanI 757 GGTACC G^G[CT][GA]CC
BclI 641 TGATCA T^GATCA
BsaJI 620 CCATGG C^C[ACGT][ACGT]GG
BsaWI 607 TCCGGA [AT]^CCGG[AT]
BseAI 607 TCCGGA T^CCGGA
BseRI 489 GAGGAG GAGGAG(10/8)
BsmAI 492 GAGAC GTCTC(1/5)
BsmFI 383 GTCCC GGGAC(10/14)
BspEI 607 TCCGGA T^CCGGA
BsrI 792 CCAGT ACTGG(1/-1)
BstF5I 207 GGATG GGATG(2/0)
BstXI 236 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 680 GGATCC [GA]^GATC[CT]
Bsu36I 675 CCTAAGG CC^T[ACGT]AGG
Cfr13I 908 GGGCC G^G[ACGT]CC
DsaI 620 CCATGG C^C[GA][CT]GG
Eco81I 675 CCTAAGG CC^T[ACGT]AGG
EcoT14I 620 CCATGG C^C[AT][AT]GG
FbaI 641 TGATCA T^GATCA
FokI 207 GGATG GGATG(9/13)
HapII 608 CCGG C^CGG
HpaII 608 CCGG C^CGG
KpnI 757 GGTACC GGTAC^C
MaeII 887 ACGT A^CGT
MfeI 227 CAATTG C^AATTG
MflI 680 GGATCC [GA]^GATC[CT]
MroI 607 TCCGGA T^CCGGA
MspA1I 189 CAGCTG C[AC]G^C[GT]G
MspI 608 CCGG C^CGG
MunI 227 CAATTG C^AATTG
NcoI 620 CCATGG C^CATGG
NheI 670 GCTAGC G^CTAGC
PshBI 468 ATTAAT AT^TAAT
PvuII 189 CAGCTG CAG^CTG
Sau96I 908 GGGCC G^G[ACGT]CC
SfcI 398 CTGTAG C^T[GA][CT]AG
SmlI 486 CTTGAG C^T[CT][GA]AG
StyI 620 CCATGG C^C[AT][AT]GG
XhoII 680 GGATCC [GA]^GATC[CT]
XmnI 921 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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BalI 371 TGGCCA TGG^CCA
623 TGGCCA
BfaI 671 CTAG C^TAG
838 CTAG
BsrDI 615 CATTGC GCAATG(2/0)
660 CATTGC
BssKI 93 CCTGG ^CC[ACGT]GG
255 CCTGG
BstNI 93 CCTGG CC^[AT]GG
255 CCTGG
EaeI 371 TGGCCA [CT]^GGCC[GA]
623 TGGCCA
EcoNI 113 CCTTGTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
500 CCTTTTGTAGG
EcoRII 93 CCTGG ^CC[AT]GG
255 CCTGG
HincII 460 GTTAAC GT[CT]^[GA]AC
597 GTTAAC
HindII 460 GTTAAC GT[CT]^[GA]AC
597 GTTAAC
HpaI 460 GTTAAC GTT^AAC
597 GTTAAC
MaeI 671 CTAG C^TAG
838 CTAG
MboII 530 TCTTC GAAGA(8/7)
656 TCTTC
MluNI 371 TGGCCA TGG^CCA
623 TGGCCA
MscI 371 TGGCCA TGG^CCA
623 TGGCCA
MvaI 93 CCTGG CC^[AT]GG
255 CCTGG
NlaIV 680 GGATCC GG[ACGT]^[ACGT]CC
757 GGTACC
PleI 381 GAGTC GAGTC(4/5)
611 GAGTC
ScrFI 93 CCTGG CC^[ACGT]GG
255 CCTGG
SfaNI 208 GATGC GCATC(5/9)
691 GCATC
TfiI 123 GAATC G^A[AT]TC
843 GATTC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI AluI AlwI BsiYI BslI
Cac8I DdeI DpnII HaeIII HinfI
HphI MaeIII MboI MnlI MseI
MslI NdeII NlaIII RsaI Sau3AI
Tru9I Tsp45I Tsp509I TspRI