################################## # NO CUT ENZYMES ( 207 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DdeI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI MwoI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 67 GAATTC [GA]^AATT[CT] AgeI 189 ACCGGT A^CCGGT AluI 8 AGCT AG^CT ApoI 67 GAATTC [GA]^AATT[CT] AvaII 192 GGTCC G^G[AT]CC BbvI 9 GCTGC GCAGC(8/12) BcnI 144 CCCGG CC^[GC]GG BmyI 141 GTGCCC G[AGT]GC[ACT]^C BsaWI 189 ACCGGT [AT]^CCGG[AT] BsgI 111 CTGCAC GTGCAG(16/14) BsmAI 164 GTCTC GTCTC(1/5) Bsp1286I 141 GTGCCC G[AGT]GC[ACT]^C BsrFI 189 ACCGGT [GA]^CCGG[CT] BstF5I 147 GGATG GGATG(2/0) BstNI 33 CCTGG CC^[AT]GG Cac8I 97 GCCTGC GC[ACGT]^[ACGT]GC Cfr10I 189 ACCGGT [GA]^CCGG[CT] DraII 94 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 94 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 67 GAATTC G^AATTC EcoRII 33 CCTGG ^CC[AT]GG EcoT22I 173 ATGCAT ATGCA^T Fnu4HI 9 GCTGC GC^[ACGT]GC FokI 147 GGATG GGATG(9/13) HaeIII 96 GGCC GG^CC HgaI 151 GCGTC GACGC(5/10) ItaI 9 GCTGC GC^[ACGT]GC MnlI 184 CCTC CCTC(7/6) MslI 202 CATGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 7 CAGCTG C[AC]G^C[GT]G MvaI 33 CCTGG CC^[AT]GG NciI 144 CCCGG CC^[GC]GG NsiI 173 ATGCAT ATGCA^T NspI 131 ACATGC [GA]CATG^[CT] PinAI 189 ACCGGT A^CCGGT PvuII 7 CAGCTG CAG^CTG SfaNI 148 GATGC GCATC(5/9) TseI 9 GCTGC G^C[AT]GC Tsp509I 68 AATT ^AATT ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsiYI 33 CCTGGTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 138 CCTGTGCCCGG BslI 33 CCTGGTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 138 CCTGTGCCCGG BsmFI 153 GTCCC GGGAC(10/14) 193 GTCCC BssKI 33 CCTGG ^CC[ACGT]GG 144 CCCGG Cfr13I 95 GGGCC G^G[ACGT]CC 192 GGTCC HapII 145 CCGG C^CGG 190 CCGG HpaII 145 CCGG C^CGG 190 CCGG MspI 145 CCGG C^CGG 190 CCGG NlaIV 94 GGGGCC GG[ACGT]^[ACGT]CC 192 GGTCCC Sau96I 95 GGGCC G^G[ACGT]CC 192 GGTCC ScrFI 33 CCTGG CC^[ACGT]GG 144 CCCGG ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### NlaIII