Restriction map for
pFN21AE0278
(Linear) (Six-base) MAPSORT of: KIEE0278.seq Check: 7809 from: 1 to: 633
KIEE0278.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 604 633
Size: 604 29
AccI GT'mk_AC
Cuts at: 0 536 633
Size: 536 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 633
Size: 223 410
ApaI G_GGCC'C
Cuts at: 0 79 550 633
Size: 79 471 83
Fragments arranged by size:
471 83 79
BanII G_rGCy'C
Cuts at: 0 79 550 633
Size: 79 471 83
Fragments arranged by size:
471 83 79
BclI T'GATC_A
Cuts at: 0 387 633
Size: 387 246
BfrBI ATG'CAT
Cuts at: 0 461 633
Size: 461 172
Bme1580I G_kGCm'C
Cuts at: 0 79 550 633
Size: 79 471 83
Fragments arranged by size:
471 83 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 633
Size: 96 537
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 246 526 601 633
Size: 246 280 75 32
Fragments arranged by size:
280 246 75 32
BseYI C'CCAG_C
Cuts at: 0 276 612 633
Size: 276 336 21
Fragments arranged by size:
336 276 21
Bsp1286I G_dGCh'C
Cuts at: 0 79 550 633
Size: 79 471 83
Fragments arranged by size:
471 83 79
BspHI T'CATG_A
Cuts at: 0 491 633
Size: 491 142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 604 633
Size: 604 29
BstEII G'GTnAC_C
Cuts at: 0 591 633
Size: 591 42
Bsu36I CC'TnA_GG
Cuts at: 0 584 633
Size: 584 49
BtgI C'CryG_G
Cuts at: 0 146 209 285 519 633
Size: 146 63 76 234 114
Fragments arranged by size:
234 146 114 76 63
BtsI GCAGTG_nn'
Cuts at: 0 429 633
Size: 429 204
EaeI y'GGCC_r
Cuts at: 0 149 288 633
Size: 149 139 345
Fragments arranged by size:
345 149 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 223 633
Size: 96 127 410
Fragments arranged by size:
410 127 96
EcoO109I rG'GnC_Cy
Cuts at: 0 75 89 546 547 633
Size: 75 14 457 1 86
Fragments arranged by size:
457 86 75 14 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 156 633
Size: 156 477
HaeII r_GCGC'y
Cuts at: 0 239 619 633
Size: 239 380 14
Fragments arranged by size:
380 239 14
MscI TGG'CCA
Cuts at: 0 151 290 633
Size: 151 139 343
Fragments arranged by size:
343 151 139
MslI CAynn'nnrTG
Cuts at: 0 371 462 633
Size: 371 91 171
Fragments arranged by size:
371 171 91
NcoI C'CATG_G
Cuts at: 0 209 633
Size: 209 424
NsiI A_TGCA'T
Cuts at: 0 463 633
Size: 463 170
NspI r_CATG'y
Cuts at: 0 461 465 633
Size: 461 4 168
Fragments arranged by size:
461 168 4
PspOMI G'GGCC_C
Cuts at: 0 75 546 633
Size: 75 471 87
Fragments arranged by size:
471 87 75
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 487 519 633
Size: 487 32 114
Fragments arranged by size:
487 114 32
PstI C_TGCA'G
Cuts at: 0 424 565 601 633
Size: 424 141 36 32
Fragments arranged by size:
424 141 36 32
SfcI C'TryA_G
Cuts at: 0 420 561 597 633
Size: 420 141 36 36
Fragments arranged by size:
420 141 36 36
StyI C'CwwG_G
Cuts at: 0 209 296 357 633
Size: 209 87 61 276
Fragments arranged by size:
276 209 87 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 56 633
Size: 56 577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 303 633
Size: 120 183 330
Fragments arranged by size:
330 183 120
XmnI GAAnn'nnTTC
Cuts at: 0 136 139 633
Size: 136 3 494
Fragments arranged by size:
494 136 3
Enzymes that do cut:
AarI AccI AcuI ApaI BanII BclI BfrBI Bme1580I
BpmI BseRI BseYI Bsp1286I BspHI BspMI BstEII Bsu36I
BtgI BtsI EaeI Eco57MI EcoO109I FalI HaeII MscI
MslI NcoI NsiI NspI PspOMI PsrI PstI SfcI
StyI TaqII XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI