################################## # NO CUT ENZYMES ( 198 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HapII HincII HindII HindIII HpaI HpaII KpnI Ksp632I MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MspI MunI NaeI NciI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I TseI Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AfaI 264 GTAC GT^AC Asp700I 177 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 43 GGCGCC G^G[CT][GA]CC BbeI 43 GGCGCC GGCGC^C BfaI 31 CTAG C^TAG BglI 119 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsmFI 282 GGGAC GGGAC(10/14) BstXI 251 CCATGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 6 TGGCCG [CT]^GGCC[GA] FauI 97 CCCGC CCCGC(4/6) HinfI 86 GAATC G^A[ACGT]TC KasI 43 GGCGCC G^GCGCC MaeI 31 CTAG C^TAG MaeII 257 ACGT A^CGT NarI 43 GGCGCC GG^CGCC NcoI 251 CCATGG C^CATGG NlaIII 252 CATG CATG^ NlaIV 43 GGCGCC GG[ACGT]^[ACGT]CC RsaI 264 GTAC GT^AC SfaNI 205 GATGC GCATC(5/9) SfoI 43 GGCGCC GGC^GCC SmlI 271 CTCAAG C^T[CT][GA]AG TfiI 86 GAATC G^A[AT]TC XmnI 177 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcyI 43 GGCGCC G[GA]^CG[CT]C 127 GGCGTC BsaHI 43 GGCGCC G[GA]^CG[CT]C 127 GGCGTC BsiYI 9 CCGCGGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 105 CCGCCGCCTGG BslI 9 CCGCGGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 105 CCGCCGCCTGG Cac8I 96 GCCCGC GC[ACGT]^[ACGT]GC 115 GCTCGC EcoT14I 81 CCAAGG C^C[AT][AT]GG 251 CCATGG HaeII 43 GGCGCC [GA]GCGC^[CT] 275 AGCGCC HaeIII 7 GGCC GG^CC 145 GGCC Hin1I 43 GGCGCC G[GA]^CG[CT]C 127 GGCGTC KspI 9 CCGCGG CCGC^GG 120 CCGCGG MnlI 147 CCTC CCTC(7/6) 157 GAGG MslI 246 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 252 CATGGACGTG MspA1I 9 CCGCGG C[AC]G^C[GT]G 120 CCGCGG SacII 9 CCGCGG CCGC^GG 120 CCGCGG StyI 81 CCAAGG C^C[AT][AT]GG 251 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AccII AciI BsaJI BssKI BstNI BstUI CfoI DsaI EcoRII Fnu4HI HgaI HhaI HinP1I HphI ItaI MaeIII MvaI MvnI MwoI ScrFI Tsp45I