(Circular) (Six-base) MAPSORT of: pFN21AE0267.seq Check: 1176 from: 1 to: 5650 pFN21AE0267 5650 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2878 2878 Size: 5650 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2444 3460 278 Size: 53 83 186 936 908 1016 2468 Fragments arranged by size: 2468 1016 936 908 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 1937 Size: 1056 4594 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3713 5097 5365 1632 Size: 2081 1384 268 1917 Fragments arranged by size: 2081 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 4379 4379 Size: 5650 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5650 AlwNI CAG_nnn'CTG Cuts at: 3393 4965 3393 Size: 1572 4078 ApaI G_GGCC'C Cuts at: 2038 2038 Size: 5650 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2959 3185 3337 5446 1493 Size: 1466 226 152 2109 1697 Fragments arranged by size: 2109 1697 1466 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2285 2975 1870 Size: 73 342 690 4545 Fragments arranged by size: 4545 690 342 73 BamHI G'GATC_C Cuts at: 2980 3347 2980 Size: 367 5283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2234 2281 2357 2713 2856 Size: 325 217 627 447 47 76 356 143 Cuts at: 2856 2890 2904 2971 4426 618 Size: 34 14 67 1455 1842 Fragments arranged by size: 1842 1455 627 447 356 325 217 143 76 67 47 34 14 BanII G_rGCy'C Cuts at: 1391 2038 2165 2380 2480 2678 2969 1391 Size: 647 127 215 100 198 291 4072 Fragments arranged by size: 4072 647 291 215 198 127 100 BbeI G_GCGC'C Cuts at: 2238 2285 2361 2908 2238 Size: 47 76 547 4980 Fragments arranged by size: 4980 547 76 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2239 928 Size: 971 340 4339 Fragments arranged by size: 4339 971 340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 1548 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2663 4261 136 Size: 107 122 71 631 605 991 1598 1525 Fragments arranged by size: 1598 1525 991 631 605 122 107 71 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 2038 2517 2718 2859 2895 3711 4867 1790 Size: 248 479 201 141 36 816 1156 2573 Fragments arranged by size: 2573 1156 816 479 248 201 141 36 BmgBI CAC'GTC Cuts at: 1318 2520 2596 2605 1318 Size: 1202 76 9 4363 Fragments arranged by size: 4363 1202 76 9 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3381 4339 442 Size: 416 53 83 399 24 1964 958 1753 Fragments arranged by size: 1964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2705 2781 4310 1239 Size: 830 636 76 1529 2579 Fragments arranged by size: 2579 1529 830 636 76 Bpu10I CC'TnA_GC Cuts at: 2700 2700 Size: 5650 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3775 4640 4938 3775 Size: 865 298 4487 Fragments arranged by size: 4487 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4313 882 Size: 617 2814 2219 Fragments arranged by size: 2814 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2431 3441 493 Size: 711 1227 1010 2702 Fragments arranged by size: 2702 1227 1010 711 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2235 2282 2358 2441 Size: 53 83 186 936 702 47 76 83 Cuts at: 2441 2905 3457 3839 4474 275 Size: 464 552 382 635 1451 Fragments arranged by size: 1451 936 702 635 552 464 382 186 83 83 76 53 47 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2082 2112 2255 2285 1178 Size: 30 165 30 679 30 143 30 4543 Fragments arranged by size: 4543 679 165 143 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2009 2147 1186 Size: 823 138 4689 Fragments arranged by size: 4689 823 138 BseYI C'CCAG_C Cuts at: 2029 2074 2230 2317 2456 4853 5349 2029 Size: 45 156 87 139 2397 496 2330 Fragments arranged by size: 2397 2330 496 156 139 87 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2618 2906 1163 Size: 573 582 300 288 3907 Fragments arranged by size: 3907 582 573 300 288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2367 2509 2690 3100 3861 4889 Size: 275 186 366 142 181 410 761 1028 Cuts at: 4889 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 410 366 275 186 181 142 BsiHKAI G_wGCw'C Cuts at: 2178 2678 2706 3711 3796 4867 2178 Size: 500 28 1005 85 1071 2961 Fragments arranged by size: 2961 1071 1005 500 85 28 BsmI GAATG_Cn' Cuts at: 3161 3254 3161 Size: 93 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2165 2178 2380 2480 2517 2678 Size: 399 248 127 13 202 100 37 161 Cuts at: 2678 2706 2718 2859 2895 2969 3711 3796 4867 Size: 28 12 141 36 74 742 85 1071 Cuts at: 4867 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 248 202 161 141 127 100 85 74 37 36 28 13 12 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 3539 5269 3539 Size: 1730 3920 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2401 2878 3005 844 Size: 1557 477 127 3489 Fragments arranged by size: 3489 1557 477 127 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2742 4145 4319 65 Size: 1183 1494 1403 174 1396 Fragments arranged by size: 1494 1403 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2366 4294 4481 1700 Size: 110 556 1928 187 2869 Fragments arranged by size: 2869 1928 556 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5650 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 2336 3710 4722 2336 Size: 1374 1012 3264 Fragments arranged by size: 3264 1374 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5650 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5650 BstYI r'GATC_y Cuts at: 1972 2980 3347 3732 3749 5201 5287 5299 5645 Size: 1008 367 385 17 1452 86 12 346 Cuts at: 5645 1972 Size: 1977 Fragments arranged by size: 1977 1452 1008 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2277 2277 Size: 5650 BtgI C'CryG_G Cuts at: 513 1065 2690 513 Size: 552 1625 3473 Fragments arranged by size: 3473 1625 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2239 487 Size: 683 56 1013 3898 Fragments arranged by size: 3898 1013 683 56 BtsI GCAGTG_nn' Cuts at: 2178 3166 3960 3980 2178 Size: 988 794 20 3848 Fragments arranged by size: 3848 988 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DraIII CAC_nnn'GTG Cuts at: 2173 2625 2173 Size: 452 5198 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4657 809 Size: 507 3341 1802 Fragments arranged by size: 3341 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2364 2506 2544 Size: 54 1425 50 275 18 534 142 38 Cuts at: 2544 2613 2687 2798 2941 3097 3986 5615 8 Size: 69 74 111 143 156 889 1629 43 Fragments arranged by size: 1629 1425 889 534 275 156 143 142 111 74 69 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 2364 2506 2687 3097 1537 Size: 275 552 142 181 410 4090 Fragments arranged by size: 4090 552 410 275 181 142 EarI CTCTTCn'nnn_ Cuts at: 1353 3580 1353 Size: 2227 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2276 2644 4237 4611 4757 387 Size: 1064 138 687 368 1593 374 146 1280 Fragments arranged by size: 1593 1280 1064 687 374 368 146 138 EcoICRI GAG'CTC Cuts at: 2676 2676 Size: 5650 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2705 2781 3713 4310 5097 5365 Size: 393 437 636 76 932 597 787 268 Cuts at: 5365 1239 Size: 1524 Fragments arranged by size: 1524 932 787 636 597 437 393 268 76 EcoNI CCTnn'n_nnAGG Cuts at: 2204 2204 Size: 5650 EcoO109I rG'GnC_Cy Cuts at: 2034 2035 2034 Size: 1 5649 EcoRI G'AATT_C Cuts at: 2959 3337 2959 Size: 378 5272 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 2238 2285 2361 2453 2908 4797 1678 Size: 560 47 76 92 455 1889 2531 Fragments arranged by size: 2531 1889 560 455 92 76 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 2636 2668 4291 Size: 32 135 32 683 32 349 32 1623 Cuts at: 4291 4323 4365 4397 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1623 683 349 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KasI G'GCGC_C Cuts at: 2234 2281 2357 2904 2234 Size: 47 76 547 4980 Fragments arranged by size: 4980 547 76 47 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2774 4764 4948 1305 Size: 1469 1990 184 2007 Fragments arranged by size: 2007 1990 1469 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2615 2800 2943 10 Size: 54 1425 343 783 185 143 2717 Fragments arranged by size: 2717 1425 783 343 185 143 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2621 3608 3967 4126 518 Size: 648 843 612 987 359 159 2042 Fragments arranged by size: 2042 987 843 648 612 359 159 MspA1I CmG'CkG Cuts at: 1606 2692 3066 3743 4891 5136 1606 Size: 1086 374 677 1148 245 2120 Fragments arranged by size: 2120 1148 1086 677 374 245 NaeI GCC'GGC Cuts at: 2368 2368 Size: 5650 NarI GG'CG_CC Cuts at: 2235 2282 2358 2905 2235 Size: 47 76 547 4980 Fragments arranged by size: 4980 547 76 47 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5098 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NgoMIV G'CCGG_C Cuts at: 2366 2366 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 3008 4553 1132 Size: 97 1779 1545 2229 Fragments arranged by size: 2229 1779 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5650 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5650 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5650 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PspOMI G'GGCC_C Cuts at: 2034 2034 Size: 5650 PstI C_TGCA'G Cuts at: 830 2205 3002 830 Size: 1375 797 3478 Fragments arranged by size: 3478 1375 797 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 SacI G_AGCT'C Cuts at: 2678 2678 Size: 5650 SacII CC_GC'GG Cuts at: 2693 2693 Size: 5650 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 3898 5610 3898 Size: 1712 3938 SexAI A'CCwGG_T Cuts at: 2169 2169 Size: 5650 SfcI C'TryA_G Cuts at: 826 1045 2201 2998 4133 4814 5005 826 Size: 219 1156 797 1135 681 191 1471 Fragments arranged by size: 1471 1156 1135 797 681 219 191 SfoI GGC'GCC Cuts at: 2236 2283 2359 2906 2236 Size: 47 76 547 4980 Fragments arranged by size: 4980 547 76 47 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2977 1872 Size: 1105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3754 4655 4917 820 Size: 197 926 1811 901 262 1553 Fragments arranged by size: 1811 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 2929 1517 Size: 1412 4238 StyI C'CwwG_G Cuts at: 513 1065 2241 3089 513 Size: 552 1176 848 3074 Fragments arranged by size: 3074 1176 848 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3677 3694 3847 4032 4477 325 Size: 1255 2097 17 153 185 445 1498 Fragments arranged by size: 2097 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3896 5608 96 Size: 274 80 33 51 1443 1919 1712 138 Fragments arranged by size: 1919 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2082 2114 5610 5642 2082 Size: 32 3496 32 2090 Fragments arranged by size: 3496 2090 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2554 2629 2874 2554 Size: 75 245 5330 Fragments arranged by size: 5330 245 75 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2975 1870 Size: 1105 4545 XmnI GAAnn'nnTTC Cuts at: 3779 3779 Size: 5650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2442 3458 276 Size: 53 83 186 936 908 1016 2468 Fragments arranged by size: 2468 1016 936 908 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SfiI SrfI SwaI