Restriction map for
pFN21AE0267
(Linear) (Six-base) MAPSORT of: KIEE0267.seq Check: 6109 from: 1 to: 945
KIEE0267.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 873 945
Size: 873 72
AatII G_ACGT'C
Cuts at: 0 439 945
Size: 439 506
ApaI G_GGCC'C
Cuts at: 0 33 945
Size: 33 912
AvaI C'yCGr_G
Cuts at: 0 280 945
Size: 280 665
BanI G'GyrC_C
Cuts at: 0 229 276 352 708 851 885 899 945
Size: 229 47 76 356 143 34 14 46
Fragments arranged by size:
356 229 143 76 47 46 34 14
BanII G_rGCy'C
Cuts at: 0 33 160 375 475 673 945
Size: 33 127 215 100 198 272
Fragments arranged by size:
272 215 198 127 100 33
BbeI G_GCGC'C
Cuts at: 0 233 280 356 903 945
Size: 233 47 76 547 42
Fragments arranged by size:
547 233 76 47 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 234 945
Size: 234 711
BglI GCCn_nnn'nGGC
Cuts at: 0 658 945
Size: 658 287
Bme1580I G_kGCm'C
Cuts at: 0 33 512 713 854 890 945
Size: 33 479 201 141 36 55
Fragments arranged by size:
479 201 141 55 36 33
BmgBI CAC'GTC
Cuts at: 0 515 591 600 945
Size: 515 76 9 345
Fragments arranged by size:
515 345 76 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 700 776 945
Size: 64 636 76 169
Fragments arranged by size:
636 169 76 64
Bpu10I CC'TnA_GC
Cuts at: 0 695 945
Size: 695 250
BsaAI yAC'GTr
Cuts at: 0 426 945
Size: 426 519
BsaHI Gr'CG_yC
Cuts at: 0 230 277 353 436 900 945
Size: 230 47 76 83 464 45
Fragments arranged by size:
464 230 83 76 47 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 77 107 250 280 945
Size: 77 30 143 30 665
Fragments arranged by size:
665 143 77 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4 142 945
Size: 4 138 803
Fragments arranged by size:
803 138 4
BseYI C'CCAG_C
Cuts at: 0 24 69 225 312 451 945
Size: 24 45 156 87 139 494
Fragments arranged by size:
494 156 139 87 45 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 613 901 945
Size: 313 300 288 44
Fragments arranged by size:
313 300 288 44
BsiEI CG_ry'CG
Cuts at: 0 362 504 685 945
Size: 362 142 181 260
Fragments arranged by size:
362 260 181 142
BsiHKAI G_wGCw'C
Cuts at: 0 173 673 701 945
Size: 173 500 28 244
Fragments arranged by size:
500 244 173 28
Bsp1286I G_dGCh'C
Cuts at: 0 33 160 173 375 475 512 673 701
Size: 33 127 13 202 100 37 161 28
Cuts at: 701 713 854 890 945
Size: 12 141 36 55
Fragments arranged by size:
202 161 141 127 100 55 37 36
33 28 13 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 396 873 945
Size: 396 477 72
Fragments arranged by size:
477 396 72
BsrDI GCAATG_nn'
Cuts at: 0 737 945
Size: 737 208
BsrFI r'CCGG_y
Cuts at: 0 361 945
Size: 361 584
BssSI C'ACGA_G
Cuts at: 0 331 945
Size: 331 614
Bsu36I CC'TnA_GG
Cuts at: 0 272 945
Size: 272 673
BtgI C'CryG_G
Cuts at: 0 685 945
Size: 685 260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 234 945
Size: 234 711
BtsI GCAGTG_nn'
Cuts at: 0 173 945
Size: 173 772
DraIII CAC_nnn'GTG
Cuts at: 0 168 620 945
Size: 168 452 325
Fragments arranged by size:
452 325 168
EaeI y'GGCC_r
Cuts at: 0 359 501 539 608 682 793 936 945
Size: 359 142 38 69 74 111 143 9
Fragments arranged by size:
359 143 142 111 74 69 38 9
EagI C'GGCC_G
Cuts at: 0 359 501 682 945
Size: 359 142 181 263
Fragments arranged by size:
359 263 181 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 271 639 945
Size: 271 368 306
Fragments arranged by size:
368 306 271
EcoICRI GAG'CTC
Cuts at: 0 671 945
Size: 671 274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 700 776 945
Size: 64 636 76 169
Fragments arranged by size:
636 169 76 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 945
Size: 199 746
EcoO109I rG'GnC_Cy
Cuts at: 0 29 30 945
Size: 29 1 915
Fragments arranged by size:
915 29 1
HaeII r_GCGC'y
Cuts at: 0 233 280 356 448 903 945
Size: 233 47 76 92 455 42
Fragments arranged by size:
455 233 92 76 47 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 631 663 945
Size: 250 32 349 32 282
Fragments arranged by size:
349 282 250 32 32
KasI G'GCGC_C
Cuts at: 0 229 276 352 899 945
Size: 229 47 76 547 46
Fragments arranged by size:
547 229 76 47 46
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 945
Size: 769 176
MscI TGG'CCA
Cuts at: 0 610 795 938 945
Size: 610 185 143 7
Fragments arranged by size:
610 185 143 7
MslI CAynn'nnrTG
Cuts at: 0 616 945
Size: 616 329
MspA1I CmG'CkG
Cuts at: 0 687 945
Size: 687 258
NaeI GCC'GGC
Cuts at: 0 363 945
Size: 363 582
NarI GG'CG_CC
Cuts at: 0 230 277 353 900 945
Size: 230 47 76 547 45
Fragments arranged by size:
547 230 76 47 45
NgoMIV G'CCGG_C
Cuts at: 0 361 945
Size: 361 584
PspOMI G'GGCC_C
Cuts at: 0 29 945
Size: 29 916
PstI C_TGCA'G
Cuts at: 0 200 945
Size: 200 745
SacI G_AGCT'C
Cuts at: 0 673 945
Size: 673 272
SacII CC_GC'GG
Cuts at: 0 688 945
Size: 688 257
SexAI A'CCwGG_T
Cuts at: 0 164 945
Size: 164 781
SfcI C'TryA_G
Cuts at: 0 196 945
Size: 196 749
SfoI GGC'GCC
Cuts at: 0 231 278 354 901 945
Size: 231 47 76 547 44
Fragments arranged by size:
547 231 76 47 44
StuI AGG'CCT
Cuts at: 0 924 945
Size: 924 21
StyI C'CwwG_G
Cuts at: 0 236 945
Size: 236 709
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 77 109 945
Size: 77 32 836
Fragments arranged by size:
836 77 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 549 624 869 945
Size: 549 75 245 76
Fragments arranged by size:
549 245 76 75
ZraI GAC'GTC
Cuts at: 0 437 945
Size: 437 508
Enzymes that do cut:
AarI AatII ApaI AvaI BanI BanII BbeI BbsI
BglI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BssSI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
PspOMI PstI SacI SacII SexAI SfcI SfoI StuI
StyI TstI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI BpuEI
BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI
BstZ17I ClaI CspCI DraI DrdI EarI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SalI SanDI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI