Restriction map for
pFN21AE0266
(Linear) (Six-base) MAPSORT of: KIEE0266.seq Check: 9254 from: 1 to: 1350
KIEE0266.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 785 1350
Size: 785 565
AatII G_ACGT'C
Cuts at: 0 677 1350
Size: 677 673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1136 1350
Size: 1136 214
AfeI AGC'GCT
Cuts at: 0 1240 1350
Size: 1240 110
AflIII A'CryG_T
Cuts at: 0 716 1350
Size: 716 634
AleI CACnn'nnGTG
Cuts at: 0 397 1350
Size: 397 953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 745 1350
Size: 745 605
AlwNI CAG_nnn'CTG
Cuts at: 0 162 225 849 1347 1350
Size: 162 63 624 498 3
Fragments arranged by size:
624 498 162 63 3
ApaI G_GGCC'C
Cuts at: 0 1014 1144 1350
Size: 1014 130 206
Fragments arranged by size:
1014 206 130
BanI G'GyrC_C
Cuts at: 0 418 536 1350
Size: 418 118 814
Fragments arranged by size:
814 418 118
BanII G_rGCy'C
Cuts at: 0 8 665 1014 1067 1144 1350
Size: 8 657 349 53 77 206
Fragments arranged by size:
657 349 206 77 53 8
BbeI G_GCGC'C
Cuts at: 0 422 1350
Size: 422 928
BbsI GAAGACnn'nnnn_
Cuts at: 0 831 1350
Size: 831 519
BbvCI CC'TCA_GC
Cuts at: 0 22 74 1350
Size: 22 52 1276
Fragments arranged by size:
1276 52 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1148 1182 1350
Size: 1148 34 168
Fragments arranged by size:
1148 168 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 236 1350
Size: 236 1114
BglI GCCn_nnn'nGGC
Cuts at: 0 416 577 627 1071 1350
Size: 416 161 50 444 279
Fragments arranged by size:
444 416 279 161 50
Bme1580I G_kGCm'C
Cuts at: 0 132 539 1014 1144 1350
Size: 132 407 475 130 206
Fragments arranged by size:
475 407 206 132 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 974 985 1101 1350
Size: 974 11 116 249
Fragments arranged by size:
974 249 116 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 362 1350
Size: 196 166 988
Fragments arranged by size:
988 196 166
Bpu10I CC'TnA_GC
Cuts at: 0 22 74 1350
Size: 22 52 1276
Fragments arranged by size:
1276 52 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1350
Size: 76 1274
BsaI GGTCTCn'nnnn_
Cuts at: 0 129 1350
Size: 129 1221
BsaAI yAC'GTr
Cuts at: 0 717 1194 1350
Size: 717 477 156
Fragments arranged by size:
717 477 156
BsaHI Gr'CG_yC
Cuts at: 0 419 491 674 812 1350
Size: 419 72 183 138 538
Fragments arranged by size:
538 419 183 138 72
BsaWI w'CCGG_w
Cuts at: 0 8 1350
Size: 8 1342
BseYI C'CCAG_C
Cuts at: 0 319 428 494 612 807 1020 1134 1235
Size: 319 109 66 118 195 213 114 101
Cuts at: 1235 1350
Size: 115
Fragments arranged by size:
319 213 195 118 115 114 109 101
66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1171 1350
Size: 1171 179
BsiEI CG_ry'CG
Cuts at: 0 556 875 1350
Size: 556 319 475
Fragments arranged by size:
556 475 319
BsiHKAI G_wGCw'C
Cuts at: 0 641 746 1350
Size: 641 105 604
Fragments arranged by size:
641 604 105
Bsp1286I G_dGCh'C
Cuts at: 0 8 132 539 641 665 746 1014 1067
Size: 8 124 407 102 24 81 268 53
Cuts at: 1067 1144 1350
Size: 77 206
Fragments arranged by size:
407 268 206 124 102 81 77 53
24 8
BspEI T'CCGG_A
Cuts at: 0 8 1350
Size: 8 1342
BspHI T'CATG_A
Cuts at: 0 956 1319 1350
Size: 956 363 31
Fragments arranged by size:
956 363 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 734 785 971 1350
Size: 734 51 186 379
Fragments arranged by size:
734 379 186 51
BsrBI CCG'CTC
Cuts at: 0 765 888 1350
Size: 765 123 462
Fragments arranged by size:
765 462 123
BsrDI GCAATG_nn'
Cuts at: 0 1202 1350
Size: 1202 148
BsrFI r'CCGG_y
Cuts at: 0 80 438 621 870 1350
Size: 80 358 183 249 480
Fragments arranged by size:
480 358 249 183 80
BssSI C'ACGA_G
Cuts at: 0 456 1150 1350
Size: 456 694 200
Fragments arranged by size:
694 456 200
BstAPI GCAn_nnn'nTGC
Cuts at: 0 458 1350
Size: 458 892
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1314 1350
Size: 1314 36
BtgI C'CryG_G
Cuts at: 0 920 1307 1350
Size: 920 387 43
Fragments arranged by size:
920 387 43
BtsI GCAGTG_nn'
Cuts at: 0 498 1350
Size: 498 852
EaeI y'GGCC_r
Cuts at: 0 337 440 553 854 872 908 1292 1350
Size: 337 103 113 301 18 36 384 58
Fragments arranged by size:
384 337 301 113 103 58 36 18
EagI C'GGCC_G
Cuts at: 0 553 872 1350
Size: 553 319 478
Fragments arranged by size:
553 478 319
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 375 595 976 1350
Size: 375 220 381 374
Fragments arranged by size:
381 375 374 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 362 1136 1350
Size: 196 166 774 214
Fragments arranged by size:
774 214 196 166
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1005 1118 1350
Size: 1005 113 232
Fragments arranged by size:
1005 232 113
EcoO109I rG'GnC_Cy
Cuts at: 0 279 1010 1011 1038 1141 1344 1350
Size: 279 731 1 27 103 203 6
Fragments arranged by size:
731 279 203 103 27 6 1
HaeII r_GCGC'y
Cuts at: 0 422 566 832 1242 1263 1350
Size: 422 144 266 410 21 87
Fragments arranged by size:
422 410 266 144 87 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 219 251 787 819 1350
Size: 219 32 536 32 531
Fragments arranged by size:
536 531 219 32 32
KasI G'GCGC_C
Cuts at: 0 418 1350
Size: 418 932
MscI TGG'CCA
Cuts at: 0 910 1294 1350
Size: 910 384 56
Fragments arranged by size:
910 384 56
MslI CAynn'nnrTG
Cuts at: 0 202 397 971 1350
Size: 202 195 574 379
Fragments arranged by size:
574 379 202 195
MspA1I CmG'CkG
Cuts at: 0 1138 1350
Size: 1138 212
NaeI GCC'GGC
Cuts at: 0 82 440 623 1350
Size: 82 358 183 727
Fragments arranged by size:
727 358 183 82
NarI GG'CG_CC
Cuts at: 0 419 1350
Size: 419 931
NcoI C'CATG_G
Cuts at: 0 920 1307 1350
Size: 920 387 43
Fragments arranged by size:
920 387 43
NgoMIV G'CCGG_C
Cuts at: 0 80 438 621 1350
Size: 80 358 183 729
Fragments arranged by size:
729 358 183 80
NotI GC'GGCC_GC
Cuts at: 0 553 1350
Size: 553 797
NspI r_CATG'y
Cuts at: 0 621 867 1350
Size: 621 246 483
Fragments arranged by size:
621 483 246
PfoI T'CCnGG_A
Cuts at: 0 520 1350
Size: 520 830
PpuMI rG'GwC_Cy
Cuts at: 0 279 1350
Size: 279 1071
PshAI GACnn'nnGTC
Cuts at: 0 922 1350
Size: 922 428
PspOMI G'GGCC_C
Cuts at: 0 1010 1140 1350
Size: 1010 130 210
Fragments arranged by size:
1010 210 130
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 952 984 1350
Size: 952 32 366
Fragments arranged by size:
952 366 32
PstI C_TGCA'G
Cuts at: 0 60 570 1350
Size: 60 510 780
Fragments arranged by size:
780 510 60
SanDI GG'GwC_CC
Cuts at: 0 279 1350
Size: 279 1071
ScaI AGT'ACT
Cuts at: 0 901 1350
Size: 901 449
SexAI A'CCwGG_T
Cuts at: 0 218 1350
Size: 218 1132
SfcI C'TryA_G
Cuts at: 0 56 566 1350
Size: 56 510 784
Fragments arranged by size:
784 510 56
SfoI GGC'GCC
Cuts at: 0 420 1350
Size: 420 930
SmlI C'TyrA_G
Cuts at: 0 91 1350
Size: 91 1259
SphI G_CATG'C
Cuts at: 0 621 867 1350
Size: 621 246 483
Fragments arranged by size:
621 483 246
StyI C'CwwG_G
Cuts at: 0 920 1307 1350
Size: 920 387 43
Fragments arranged by size:
920 387 43
TatI w'GTAC_w
Cuts at: 0 899 1350
Size: 899 451
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 932 964 1350
Size: 932 32 386
Fragments arranged by size:
932 386 32
Tth111I GACn'n_nGTC
Cuts at: 0 186 1350
Size: 186 1164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 918 998 1350
Size: 396 522 80 352
Fragments arranged by size:
522 396 352 80
ZraI GAC'GTC
Cuts at: 0 675 1350
Size: 675 675
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlfI AlwNI
ApaI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI
BtgI BtsI EaeI EagI EciI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PfoI PpuMI PshAI PspOMI
PsrI PstI SanDI ScaI SexAI SfcI SfoI SmlI
SphI StyI TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AloI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaBI
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PsiI PspXI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TaqII XbaI XhoI XmaI XmnI