Restriction map for
pFN21AE0246
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AlwI AocI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BanII BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MaeI MamI MboI MfeI
MflI MroI MseI MunI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI Sau3AI SbfI ScaI
SexAI SfaNI SfuI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflIII 122 ACGCGT A^C[GA][CT]GT
AhdI 1198 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 494 AGCGCT AGC^GCT
AspEI 1198 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 965 GGCGCC GGCGC^C
BbrPI 958 CACGTG CAC^GTG
BcgI 860 GCATGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseRI 79 GAGGAG GAGGAG(10/8)
BsmFI 540 GTCCC GGGAC(10/14)
BspMI 335 ACCTGC ACCTGC(4/8)
BsrDI 1229 GCAATG GCAATG(2/0)
DdeI 484 CTGAG C^T[ACGT]AG
DraII 887 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 898 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 531 GACCCTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1198 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 494 AGCGCT AGC^GCT
EcoNI 1181 CCTTCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 887 AGGCCCT [GA]G^G[ACGT]CC[CT]
HincII 715 GTCAAC GT[CT]^[GA]AC
HindII 715 GTCAAC GT[CT]^[GA]AC
HinfI 1002 GACTC G^A[ACGT]TC
KasI 965 GGCGCC G^GCGCC
KspI 423 CCGCGG CCGC^GG
MluI 122 ACGCGT A^CGCGT
NarI 965 GGCGCC GG^CGCC
PaeR7I 1185 CTCGAG C^TCGAG
PleI 1002 GACTC GAGTC(4/5)
PmaCI 958 CACGTG CAC^GTG
PmlI 958 CACGTG CAC^GTG
SacII 423 CCGCGG CCGC^GG
SapI 216 GCTCTTC GCTCTTC(1/4)
SfiI 1128 GGCCACACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 965 GGCGCC GGC^GCC
SgrAI 654 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1185 CTCGAG C^T[CT][GA]AG
XhoI 1185 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwNI 283 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
886 CAGGCCCTG
AvaII 759 GGTCC G^G[AT]CC
882 GGTCC
BglI 589 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1129 GCCACACTGGC
BpmI 1224 CTCCAG CTGGAG(16/14)
76 CTGGAG
BsaAI 914 TACGTG [CT]AC^GT[GA]
958 CACGTG
BsgI 450 CTGCAC GTGCAG(16/14)
917 GTGCAG
BsmAI 1243 GAGAC GTCTC(1/5)
1099 GTCTC
BsmBI 1098 CGTCTC CGTCTC(1/5)
1243 GAGACG
Bsp1407I 371 TGTACA T^GTACA
1088 TGTACA
BsrGI 371 TGTACA T^GTACA
1088 TGTACA
BsrI 75 ACTGG ACTGG(1/-1)
1134 ACTGG
BstAPI 282 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
897 GCACGGCGTGC
BstEII 195 GGTGACC G^GT[ACGT]ACC
1041 GGTGACC
BstPI 195 GGTGACC G^GT[ACGT]ACC
1041 GGTGACC
BstXI 185 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
356 CCATGCCCGTGG
EagI 41 CGGCCG C^GGCCG
809 CGGCCG
EclXI 41 CGGCCG C^GGCCG
809 CGGCCG
Eco52I 41 CGGCCG C^GGCCG
809 CGGCCG
EcoO65I 195 GGTGACC G^GT[ACGT]ACC
1041 GGTGACC
FauI 1238 CCCGC CCCGC(4/6)
60 GCGGG
MspA1I 58 CAGCGG C[AC]G^C[GT]G
423 CCGCGG
NcoI 617 CCATGG C^CATGG
743 CCATGG
PstI 280 CTGCAG CTGCA^G
289 CTGCAG
SfcI 280 CTGCAG C^T[GA][CT]AG
289 CTGCAG
SmaI 30 CCCGGG CCC^GGG
96 CCCGGG
SspBI 371 TGTACA T^GTACA
1088 TGTACA
TaqI 869 TCGA T^CGA
1186 TCGA
TthHB8I 869 TCGA T^CGA
1186 TCGA
XcmI 149 CCAGCAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
479 CCAGCCTGAGTGTGG
XmaCI 30 CCCGGG C^CCGGG
96 CCCGGG
XmaI 30 CCCGGG C^CCGGG
96 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I ApaLI AspHI AvaI BalI
BanI BbvI BcnI BmyI BsaHI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DsaI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboII MluNI MnlI MscI
MslI MspI MvaI MvnI MwoI
NaeI NciI NgoMIV NlaIII NlaIV
RsaI Sau96I ScrFI StyI TseI
Tsp45I TspRI