(Circular) (Six-base) MAPSORT of: pFN21AE0246.seq Check: 2729 from: 1 to: 5959 pFN21AE0246 5959 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4022 5406 5674 1632 Size: 2390 1384 268 1917 Fragments arranged by size: 2390 1917 1384 268 AfeI AGC'GCT Cuts at: 2501 2501 Size: 5959 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 2127 4858 5835 1203 Size: 924 2731 977 1327 Fragments arranged by size: 2731 1327 977 924 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 3208 4688 3208 Size: 1480 4479 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 2293 2896 3702 5274 2293 Size: 603 806 1572 2978 Fragments arranged by size: 2978 1572 806 603 ApaLI G'TGCA_C Cuts at: 2393 2674 2833 4016 5172 2393 Size: 281 159 1183 1156 3180 Fragments arranged by size: 3180 1183 1156 281 159 ApoI r'AATT_y Cuts at: 1493 3268 3494 3646 5755 1493 Size: 1775 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2035 2101 3190 3284 1870 Size: 73 92 66 1089 94 4545 Fragments arranged by size: 4545 1089 94 92 73 66 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2149 2605 2970 3280 4735 Size: 325 217 627 362 456 365 310 1455 Cuts at: 4735 618 Size: 1842 Fragments arranged by size: 1842 1455 627 456 365 362 325 310 217 BanII G_rGCy'C Cuts at: 1391 3278 1391 Size: 1887 4072 BbeI G_GCGC'C Cuts at: 2974 2974 Size: 5959 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2854 2888 4150 4184 1903 Size: 34 50 34 833 34 1262 34 3678 Fragments arranged by size: 3678 1262 833 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2600 3140 4570 Size: 107 122 71 631 605 928 540 1430 Cuts at: 4570 136 Size: 1525 Fragments arranged by size: 1525 1430 928 631 605 540 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2042 2237 2397 2678 2837 4020 5176 1790 Size: 252 195 160 281 159 1183 1156 2573 Fragments arranged by size: 2573 1183 1156 281 252 195 160 159 BmgBI CAC'GTC Cuts at: 1318 2170 1318 Size: 852 5107 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 3214 4619 1239 Size: 863 1112 1405 2579 Fragments arranged by size: 2579 1405 1112 863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2921 2965 3750 493 Size: 711 1717 44 785 2702 Fragments arranged by size: 2702 1717 785 711 44 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2418 2451 2971 3766 Size: 53 83 186 936 885 33 520 795 Cuts at: 3766 4148 4783 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 885 795 635 520 382 186 83 53 33 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 3056 3086 3212 Size: 30 165 30 768 30 855 30 126 Cuts at: 3212 3242 1178 Size: 30 3895 Fragments arranged by size: 3895 855 768 165 126 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 1186 Size: 913 5046 BseYI C'CCAG_C Cuts at: 2153 5162 5658 2153 Size: 3009 496 2454 Fragments arranged by size: 3009 2454 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2440 2943 1163 Size: 573 704 503 4179 Fragments arranged by size: 4179 704 573 503 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 2817 2841 3409 4170 5198 Size: 275 186 48 768 24 568 761 1028 Cuts at: 5198 1540 Size: 2301 Fragments arranged by size: 2301 1028 768 761 568 275 186 48 24 BsiHKAI G_wGCw'C Cuts at: 2303 2397 2678 2837 2853 4020 4105 5176 2303 Size: 94 281 159 16 1167 85 1071 3086 Fragments arranged by size: 3086 1167 1071 281 159 94 85 16 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 BsmBI CGTCTCn'nnnn_ Cuts at: 3109 3242 3109 Size: 133 5826 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2237 2303 2397 2678 2837 2853 Size: 399 252 195 66 94 281 159 16 Cuts at: 2853 3278 4020 4105 5176 1391 Size: 425 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 425 399 281 252 195 159 94 85 66 16 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2349 3314 844 Size: 1505 965 3489 Fragments arranged by size: 3489 1505 965 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3241 4454 4628 65 Size: 1183 1993 1213 174 1396 Fragments arranged by size: 1993 1396 1213 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 2420 2660 4603 4790 1700 Size: 110 234 376 240 1943 187 2869 Fragments arranged by size: 2869 1943 376 240 234 187 110 BsrGI T'GTAC_A Cuts at: 96 2376 3093 96 Size: 2280 717 2962 Fragments arranged by size: 2962 2280 717 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 2293 2908 794 Size: 1499 615 3845 Fragments arranged by size: 3845 1499 615 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 2200 3046 1184 Size: 1016 846 4097 Fragments arranged by size: 4097 1016 846 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2368 2197 Size: 171 5788 BstYI r'GATC_y Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954 Size: 1317 367 385 17 1452 86 12 346 Cuts at: 5954 1972 Size: 1977 Fragments arranged by size: 1977 1452 1317 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2367 2428 2466 2622 2748 513 Size: 552 1302 61 38 156 126 3724 Fragments arranged by size: 3724 1302 552 156 126 61 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2764 2917 487 Size: 683 56 1538 153 3529 Fragments arranged by size: 3529 1538 683 153 56 BtsI GCAGTG_nn' Cuts at: 2691 2931 3008 3475 4269 4289 2691 Size: 240 77 467 794 20 4361 Fragments arranged by size: 4361 794 467 240 77 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DraIII CAC_nnn'GTG Cuts at: 2908 2908 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2542 4966 809 Size: 507 1226 2424 1802 Fragments arranged by size: 2424 1802 1226 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2358 2481 Size: 54 1425 50 275 18 216 312 123 Cuts at: 2481 2508 2650 2814 2868 3132 3406 4295 5924 Size: 27 142 164 54 264 274 889 1629 Cuts at: 5924 8 Size: 43 Fragments arranged by size: 1629 1425 889 312 275 274 264 216 164 142 123 54 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 2814 3406 1537 Size: 275 234 768 592 4090 Fragments arranged by size: 4090 768 592 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2228 3065 3170 3889 1353 Size: 875 837 105 719 3423 Fragments arranged by size: 3423 875 837 719 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3107 4546 4920 5066 387 Size: 1064 138 1518 1439 374 146 1280 Fragments arranged by size: 1518 1439 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 3214 4022 4619 5406 5674 1239 Size: 393 470 1112 808 597 787 268 1524 Fragments arranged by size: 1524 1112 808 787 597 470 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3190 3190 Size: 5959 EcoO109I rG'GnC_Cy Cuts at: 2893 2893 Size: 5959 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2093 2222 2333 2503 2577 2646 2974 5106 Size: 415 129 111 170 74 69 328 2132 Cuts at: 5106 1678 Size: 2531 Fragments arranged by size: 2531 2132 415 328 170 129 111 74 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 3194 3226 4600 Size: 32 135 32 599 32 991 32 1374 Cuts at: 4600 4632 4674 4706 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1374 991 599 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2722 3303 3549 669 Size: 988 281 784 581 246 3079 Fragments arranged by size: 3079 988 784 581 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2970 2970 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 2127 5835 2127 Size: 3708 2251 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2112 2361 5073 5257 1305 Size: 807 249 2712 184 2007 Fragments arranged by size: 2712 2007 807 249 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 2483 2510 2870 3134 Size: 54 1425 343 528 123 27 360 264 Cuts at: 3134 10 Size: 2835 Fragments arranged by size: 2835 1425 528 360 343 264 123 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2366 2390 2729 3917 4276 4435 518 Size: 648 1200 24 339 1188 359 159 2042 Fragments arranged by size: 2042 1200 1188 648 359 339 159 24 MspA1I CmG'CkG Cuts at: 1606 2065 2430 3375 4052 5200 5445 1606 Size: 459 365 945 677 1148 245 2120 Fragments arranged by size: 2120 1148 945 677 459 365 245 NaeI GCC'GGC Cuts at: 2046 2422 2662 2046 Size: 376 240 5343 Fragments arranged by size: 5343 376 240 NarI GG'CG_CC Cuts at: 2971 2971 Size: 5959 NcoI C'CATG_G Cuts at: 513 1065 2622 2748 513 Size: 552 1557 126 3724 Fragments arranged by size: 3724 1557 552 126 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NgoMIV G'CCGG_C Cuts at: 2044 2420 2660 2044 Size: 376 240 5343 Fragments arranged by size: 5343 376 240 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 2403 1348 Size: 1055 4904 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PmlI CAC'GTG Cuts at: 2965 2965 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 2289 2298 3311 830 Size: 1459 9 1013 3478 Fragments arranged by size: 3478 1459 1013 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SacII CC_GC'GG Cuts at: 2431 2431 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SapI GCTCTTCn'nnn_ Cuts at: 2228 2228 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2285 2294 3307 4442 5123 5314 826 Size: 219 1240 9 1013 1135 681 191 1471 Fragments arranged by size: 1471 1240 1135 1013 681 219 191 9 SfiI GGCCn_nnn'nGGCC Cuts at: 3140 3140 Size: 5959 SfoI GGC'GCC Cuts at: 2972 2972 Size: 5959 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 2660 1700 Size: 960 4999 SmaI CCC'GGG Cuts at: 1872 2037 2103 3286 1872 Size: 165 66 1183 4545 Fragments arranged by size: 4545 1183 165 66 SmlI C'TyrA_G Cuts at: 820 1017 1943 3190 4063 4964 5226 820 Size: 197 926 1247 873 901 262 1553 Fragments arranged by size: 1553 1247 926 901 873 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2190 2622 2748 3398 513 Size: 552 1125 432 126 650 3074 Fragments arranged by size: 3074 1125 650 552 432 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2167 2380 2752 3986 4003 4156 4341 Size: 1255 587 213 372 1234 17 153 185 Cuts at: 4341 4786 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1234 587 445 372 213 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 3093 4205 Size: 274 80 33 51 1443 399 717 1112 Cuts at: 4205 5917 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1112 717 399 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2987 3019 3212 3244 5919 5951 2987 Size: 32 193 32 2675 32 2995 Fragments arranged by size: 2995 2675 193 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2161 2491 2161 Size: 330 5629 XhoI C'TCGA_G Cuts at: 1943 3190 1943 Size: 1247 4712 XmaI C'CCGG_G Cuts at: 1870 2035 2101 3284 1870 Size: 165 66 1183 4545 Fragments arranged by size: 4545 1183 165 66 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SrfI SwaI