Restriction map for
pFN21AE0245
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsiEI BsiHKAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I CelII ClaI CpoI DpnII
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HindIII HpaI KasI Ksp632I
KspI MaeII MamI MboI MboII
MfeI MflI MluI MroI MseI
MslI MunI MvnI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
ScaI SexAI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 688 GGTACC G^GTACC
AcyI 188 GGCGTC G[GA]^CG[CT]C
AocI 366 CCTGAGG CC^T[ACGT]AGG
ApaI 392 GGGCCC GGGCC^C
Asp718I 688 GGTACC G^GTACC
AvaII 223 GGACC G^G[AT]CC
BalI 473 TGGCCA TGG^CCA
BanI 688 GGTACC G^G[CT][GA]CC
BanII 392 GGGCCC G[GA]GC[CT]^C
BbvCI 375 CCTCAGC CCTCAGC(-5/-2)
BfaI 193 CTAG C^TAG
BmyI 392 GGGCCC G[AGT]GC[ACT]^C
BsaHI 188 GGCGTC G[GA]^CG[CT]C
BsaWI 83 ACCGGA [AT]^CCGG[AT]
BsiWI 15 CGTACG C^GTACG
Bsp1286I 392 GGGCCC G[AGT]GC[ACT]^C
BspMI 225 ACCTGC ACCTGC(4/8)
BsrFI 316 ACCGGC [GA]^CCGG[CT]
BsrI 185 ACTGG ACTGG(1/-1)
Bsu36I 366 CCTGAGG CC^T[ACGT]AGG
Cfr10I 316 ACCGGC [GA]^CCGG[CT]
DsaI 489 CCACGG C^C[GA][CT]GG
Eco81I 366 CCTGAGG CC^T[ACGT]AGG
EcoNI 362 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 189 GCGTC GACGC(5/10)
Hin1I 188 GGCGTC G[GA]^CG[CT]C
HincII 274 GTCAAC GT[CT]^[GA]AC
HindII 274 GTCAAC GT[CT]^[GA]AC
HinfI 345 GACTC G^A[ACGT]TC
KpnI 688 GGTACC GGTAC^C
MaeI 193 CTAG C^TAG
MluNI 473 TGGCCA TGG^CCA
MscI 473 TGGCCA TGG^CCA
NlaIII 714 CATG CATG^
PleI 345 GACTC GAGTC(4/5)
SbfI 226 CCTGCAGG CCTGCA^GG
SfaNI 288 GCATC GCATC(5/9)
SfiI 144 GGCCGGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 226 CCTGCAGG CCTGCA^GG
TaqI 675 TCGA T^CGA
TthHB8I 675 TCGA T^CGA
XcmI 434 CCAAGGTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BglI 145 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
461 GCCCGCTGGGC
BsaI 121 GGTCTC GGTCTC(1/5)
263 GGTCTC
BseRI 4 GAGGAG GAGGAG(10/8)
523 GAGGAG
BsmFI 72 GGGAC GGGAC(10/14)
593 GGGAC
BssSI 63 CACGAG CACGAG(-5/-1)
268 CACGAG
BstEII 204 GGTCACC G^GT[ACGT]ACC
402 GGTCACC
BstPI 204 GGTCACC G^GT[ACGT]ACC
402 GGTCACC
CfoI 286 GCGC GCG^C
504 GCGC
EaeI 152 TGGCCG [CT]^GGCC[GA]
473 TGGCCA
EcoO65I 204 GGTCACC G^GT[ACGT]ACC
402 GGTCACC
EcoT14I 159 CCAAGG C^C[AT][AT]GG
434 CCAAGG
HhaI 286 GCGC GCG^C
504 GCGC
HinP1I 286 GCGC G^CGC
504 GCGC
PstI 175 CTGCAG CTGCA^G
227 CTGCAG
SfcI 175 CTGCAG C^T[GA][CT]AG
227 CTGCAG
SmaI 107 CCCGGG CCC^GGG
581 CCCGGG
StyI 159 CCAAGG C^C[AT][AT]GG
434 CCAAGG
XmaCI 107 CCCGGG C^CCGGG
581 CCCGGG
XmaI 107 CCCGGG C^CCGGG
581 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AvaI BbvI
BcnI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsoBI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeIII MnlI MspA1I MspI MvaI
MwoI NciI NlaIV PvuII RsaI
Sau96I ScrFI TseI Tsp45I TspRI