(Circular) (Six-base) MAPSORT of: pFN21AE0245.seq Check: 7509 from: 1 to: 5425 pFN21AE0245 5425 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3235 278 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 1937 Size: 831 4594 Acc65I G'GTAC_C Cuts at: 2693 2746 2693 Size: 53 5372 AclI AA'CG_TT Cuts at: 1563 3552 3925 1563 Size: 1989 373 3063 Fragments arranged by size: 3063 1989 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3488 4872 5140 1632 Size: 1856 1384 268 1917 Fragments arranged by size: 1917 1856 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5228 AflIII A'CryG_T Cuts at: 1203 4324 5301 1203 Size: 3121 977 1327 Fragments arranged by size: 3121 1327 977 AgeI A'CCGG_T Cuts at: 4256 4256 Size: 5425 AhdI GACnn_n'nnGTC Cuts at: 4154 4154 Size: 5425 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5425 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5425 AlwNI CAG_nnn'CTG Cuts at: 3168 4740 3168 Size: 1572 3853 ApaI G_GGCC'C Cuts at: 2401 2401 Size: 5425 ApaLI G'TGCA_C Cuts at: 3482 4638 3482 Size: 1156 4269 ApoI r'AATT_y Cuts at: 1493 2734 2960 3112 5221 1493 Size: 1241 226 152 2109 1697 Fragments arranged by size: 2109 1697 1241 226 152 AseI AT'TA_AT Cuts at: 160 1032 3979 160 Size: 872 2947 1606 Fragments arranged by size: 2947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 AvaI C'yCGr_G Cuts at: 1870 1943 2112 2525 2586 2750 1870 Size: 73 169 413 61 164 4545 Fragments arranged by size: 4545 413 169 164 73 61 BamHI G'GATC_C Cuts at: 2755 3122 2755 Size: 367 5058 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2693 2746 4201 618 Size: 325 217 627 906 53 1455 1842 Fragments arranged by size: 1842 1455 906 627 325 217 53 BanII G_rGCy'C Cuts at: 1391 2401 2744 1391 Size: 1010 343 4072 Fragments arranged by size: 4072 1010 343 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4454 BbvCI CC'TCA_GC Cuts at: 2381 2381 Size: 5425 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3616 3650 1903 Size: 34 1679 34 3678 Fragments arranged by size: 3678 1679 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3317 4527 3317 Size: 1210 4215 BclI T'GATC_A Cuts at: 1548 1548 Size: 5425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2156 2472 4036 Size: 107 122 71 631 605 484 316 1564 Cuts at: 4036 136 Size: 1525 Fragments arranged by size: 1564 1525 631 605 484 316 122 107 71 BglII A'GATC_T Cuts at: 5420 5420 Size: 5425 BlpI GC'TnA_GC Cuts at: 2842 2842 Size: 5425 Bme1580I G_kGCm'C Cuts at: 1790 2401 3486 4642 1790 Size: 611 1085 1156 2573 Fragments arranged by size: 2573 1156 1085 611 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5425 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3156 4114 442 Size: 416 53 83 399 24 1739 958 1753 Fragments arranged by size: 1753 1739 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2296 2555 2651 4085 1239 Size: 1057 259 96 1434 2579 Fragments arranged by size: 2579 1434 1057 259 96 Bpu10I CC'TnA_GC Cuts at: 2381 2381 Size: 5425 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3550 4415 4713 3550 Size: 865 298 4262 Fragments arranged by size: 4262 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2132 2274 4088 882 Size: 617 633 142 1814 2219 Fragments arranged by size: 2219 1814 633 617 142 BsaAI yAC'GTr Cuts at: 493 1204 3216 493 Size: 711 2012 2702 Fragments arranged by size: 2702 2012 711 BsaBI GATnn'nnATC Cuts at: 5419 5419 Size: 5425 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2194 3232 3614 4249 Size: 53 83 186 936 661 1038 382 635 Cuts at: 4249 275 Size: 1451 Fragments arranged by size: 1451 1038 936 661 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2088 3858 4256 4530 4677 5088 2088 Size: 1770 398 274 147 411 2425 Fragments arranged by size: 2425 1770 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2193 2223 2420 2450 1178 Size: 30 165 30 790 30 197 30 4153 Fragments arranged by size: 4153 790 197 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2543 1186 Size: 838 519 4068 Fragments arranged by size: 4068 838 519 BseYI C'CCAG_C Cuts at: 2074 2284 2356 2449 2470 2636 4628 5124 2074 Size: 210 72 93 21 166 1992 496 2375 Fragments arranged by size: 2375 1992 496 210 166 93 72 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2122 2171 2465 1163 Size: 573 386 49 294 4123 Fragments arranged by size: 4123 573 386 294 49 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2875 3636 4664 1540 Size: 275 186 874 761 1028 2301 Fragments arranged by size: 2301 1028 874 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3486 3571 4642 3486 Size: 85 1071 4269 Fragments arranged by size: 4269 1071 85 BsiWI C'GTAC_G Cuts at: 2020 2020 Size: 5425 BsmI GAATG_Cn' Cuts at: 2936 3029 2936 Size: 93 5332 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2401 2744 3486 3571 4642 1391 Size: 399 611 343 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 611 399 343 85 BspEI T'CCGG_A Cuts at: 5088 5088 Size: 5425 BspHI T'CATG_A Cuts at: 3314 5044 3314 Size: 1730 3695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2239 2780 844 Size: 1395 541 3489 Fragments arranged by size: 3489 1395 541 BsrBI CCG'CTC Cuts at: 1394 1823 3312 1394 Size: 429 1489 3507 Fragments arranged by size: 3507 1489 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3920 4094 65 Size: 1183 2672 174 1396 Fragments arranged by size: 2672 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2321 4069 4256 1700 Size: 110 511 1748 187 2869 Fragments arranged by size: 2869 1748 511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5425 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5425 BssSI C'ACGA_G Cuts at: 2068 2273 3485 4497 2068 Size: 205 1212 1012 2996 Fragments arranged by size: 2996 1212 1012 205 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5425 BstBI TT'CG_AA Cuts at: 4229 4229 Size: 5425 BstEII G'GTnAC_C Cuts at: 1184 2209 2407 1184 Size: 1025 198 4202 Fragments arranged by size: 4202 1025 198 BstYI r'GATC_y Cuts at: 1972 2755 3122 3507 3524 4976 5062 5074 5420 Size: 783 367 385 17 1452 86 12 346 Cuts at: 5420 1972 Size: 1977 Fragments arranged by size: 1977 1452 783 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2372 2372 Size: 5425 BtgI C'CryG_G Cuts at: 513 1065 2004 2494 513 Size: 552 939 490 3444 Fragments arranged by size: 3444 939 552 490 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4686 Fragments arranged by size: 4686 683 56 BtsI GCAGTG_nn' Cuts at: 2110 2941 3735 3755 2110 Size: 831 794 20 3780 Fragments arranged by size: 3780 831 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5390 DraI TTT'AAA Cuts at: 2729 2729 Size: 5425 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4432 809 Size: 507 3116 1802 Fragments arranged by size: 3116 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2478 2872 Size: 54 1425 50 275 18 327 321 394 Cuts at: 2872 3761 5390 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 394 327 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2872 1537 Size: 275 1060 4090 Fragments arranged by size: 4090 1060 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3355 1353 Size: 2002 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4012 4386 4532 387 Size: 1064 138 2423 374 146 1280 Fragments arranged by size: 2423 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2296 2555 2651 3488 4085 4872 5140 Size: 393 664 259 96 837 597 787 268 Cuts at: 5140 1239 Size: 1524 Fragments arranged by size: 1524 837 787 664 597 393 268 259 96 EcoNI CCTnn'n_nnAGG Cuts at: 2371 2371 Size: 5425 EcoO109I rG'GnC_Cy Cuts at: 2377 2397 2398 2377 Size: 20 1 5404 Fragments arranged by size: 5404 20 1 EcoRI G'AATT_C Cuts at: 2734 3112 2734 Size: 378 5047 FspI TGC'GCA Cuts at: 3931 3931 Size: 5425 HaeII r_GCGC'y Cuts at: 1678 4572 1678 Size: 2894 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2193 2225 2252 2284 4066 Size: 32 135 32 621 32 27 32 1782 Cuts at: 4066 4098 4140 4172 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1782 621 135 42 32 32 32 32 32 32 27 HincII GTy'rAC Cuts at: 669 1657 1938 2281 2769 3015 669 Size: 988 281 343 488 246 3079 Fragments arranged by size: 3079 988 488 343 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5425 HpaI GTT'AAC Cuts at: 3015 3015 Size: 5425 KpnI G_GTAC'C Cuts at: 2697 2750 2697 Size: 53 5372 MfeI C'AATT_G Cuts at: 3024 3024 Size: 5425 MluI A'CGCG_T Cuts at: 5301 5301 Size: 5425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4539 4723 1305 Size: 3234 184 2007 Fragments arranged by size: 3234 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2480 10 Size: 54 1425 343 648 2955 Fragments arranged by size: 2955 1425 648 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3383 3742 3901 518 Size: 648 2217 359 159 2042 Fragments arranged by size: 2217 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2185 2251 2328 2338 2356 2449 2470 2841 Size: 579 66 77 10 18 93 21 371 Cuts at: 2841 3518 4666 4911 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 579 371 245 93 77 66 21 18 10 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3934 Fragments arranged by size: 3934 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5425 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5425 NotI GC'GGCC_GC Cuts at: 2872 2872 Size: 5425 NspI r_CATG'y Cuts at: 1132 1229 2783 4328 1132 Size: 97 1554 1545 2229 Fragments arranged by size: 2229 1554 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5425 PciI A'CATG_T Cuts at: 4324 4324 Size: 5425 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5425 PmeI GTTT'AAAC Cuts at: 2729 2729 Size: 5425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3494 3526 5007 5039 3494 Size: 32 1481 32 3880 Fragments arranged by size: 3880 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5425 PsiI TTA'TAA Cuts at: 2995 2995 Size: 5425 PspOMI G'GGCC_C Cuts at: 2397 2397 Size: 5425 PstI C_TGCA'G Cuts at: 830 2184 2236 2777 830 Size: 1354 52 541 3478 Fragments arranged by size: 3478 1354 541 52 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5425 PvuII CAG'CTG Cuts at: 2185 2251 2356 2449 2185 Size: 66 105 93 5161 Fragments arranged by size: 5161 105 93 66 SalI G'TCGA_C Cuts at: 1936 2767 1936 Size: 831 4594 SbfI CC_TGCA'GG Cuts at: 2236 2777 2236 Size: 541 4884 ScaI AGT'ACT Cuts at: 3673 5385 3673 Size: 1712 3713 SfcI C'TryA_G Cuts at: 826 1045 2180 2232 2773 3908 4589 4780 826 Size: 219 1135 52 541 1135 681 191 1471 Fragments arranged by size: 1471 1135 1135 681 541 219 191 52 SfiI GGCCn_nnn'nGGCC Cuts at: 2156 2156 Size: 5425 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5425 SmaI CCC'GGG Cuts at: 1872 2114 2588 2752 1872 Size: 242 474 164 4545 Fragments arranged by size: 4545 474 242 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 3529 4430 4692 820 Size: 197 926 1586 901 262 1553 Fragments arranged by size: 1586 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3216 493 Size: 2723 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5425 SphI G_CATG'C Cuts at: 1132 2783 1132 Size: 1651 3774 SspI AAT'ATT Cuts at: 5 52 3349 5 Size: 47 3297 2081 Fragments arranged by size: 3297 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5425 StyI C'CwwG_G Cuts at: 513 1065 2004 2164 2439 2864 513 Size: 552 939 160 275 425 3074 Fragments arranged by size: 3074 939 552 425 275 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2069 2507 2603 3452 3469 3622 3807 Size: 1255 489 438 96 849 17 153 185 Cuts at: 3807 4252 325 Size: 445 1498 Fragments arranged by size: 1498 1255 849 489 445 438 185 153 96 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3671 5383 96 Size: 274 80 33 51 1443 1694 1712 138 Fragments arranged by size: 1712 1694 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5385 5417 5385 Size: 32 5393 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5425 XbaI T'CTAG_A Cuts at: 2761 2761 Size: 5425 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2446 2446 Size: 5425 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5425 XmaI C'CCGG_G Cuts at: 1870 2112 2586 2750 1870 Size: 242 474 164 4545 Fragments arranged by size: 4545 474 242 164 XmnI GAAnn'nnTTC Cuts at: 3554 3554 Size: 5425 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3233 276 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI