Restriction map for
pFN21AE2076
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# NO CUT ENZYMES ( 98 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BcgI BfrI BlnI
BlpI Bpu1102I BsaAI BsaI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BspMI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoT22I FseI
FspI HaeII HgaI HindIII KasI
KpnI KspI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SnaBI SpeI Sse8387I SwaI Tth111I
Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1652 GACGTC GACGT^C
AccII 30 CGCG CG^CG
AcyI 1652 GACGTC G[GA]^CG[CT]C
AocI 1867 CCTAAGG CC^T[ACGT]AGG
ApaI 37 GGGCCC GGGCC^C
AseI 906 ATTAAT AT^TAAT
AsnI 906 ATTAAT AT^TAAT
Asp700I 994 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 12 CCCGGG C^[CT]CG[GA]G
BalI 2151 TGGCCA TGG^CCA
BciVI 1582 GGATAC GTATCC(6/5)
BglI 9 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 511 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1652 GACGTC G[GA]^CG[CT]C
BsiEI 22 CGGCCG CG[GA][CT]^CG
BsmI 1457 GCATTC GAATGC(1/-1)
BsoBI 12 CCCGGG C^[CT]CG[GA]G
BsrFI 67 ACCGGC [GA]^CCGG[CT]
BstUI 30 CGCG CG^CG
BstXI 679 CCATTTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1867 CCTAAGG CC^T[ACGT]AGG
CfoI 29 GCGC GCG^C
Cfr10I 67 ACCGGC [GA]^CCGG[CT]
DrdI 1906 GACAATACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 22 CGGCCG C^GGCCG
EclXI 22 CGGCCG C^GGCCG
Eco52I 22 CGGCCG C^GGCCG
Eco81I 1867 CCTAAGG CC^T[ACGT]AGG
EcoRV 1503 GATATC GAT^ATC
HhaI 29 GCGC GCG^C
Hin1I 1652 GACGTC G[GA]^CG[CT]C
HinP1I 29 GCGC G^CGC
HpaI 2226 GTTAAC GTT^AAC
HphI 1758 GGTGA GGTGA(8/7)
MamI 511 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2151 TGGCCA TGG^CCA
MscI 2151 TGGCCA TGG^CCA
MspA1I 1264 CAGCTG C[AC]G^C[GT]G
MvnI 30 CGCG CG^CG
PleI 1785 GAGTC GAGTC(4/5)
PshBI 906 ATTAAT AT^TAAT
PvuII 1264 CAGCTG CAG^CTG
SacI 571 GAGCTC GAGCT^C
SapI 1635 GAAGAGC GCTCTTC(1/4)
SgrAI 66 CACCGGCG C[GA]^CCGG[CT]G
SmaI 12 CCCGGG CCC^GGG
SphI 1611 GCATGC GCATG^C
SspI 250 AATATT AAT^ATT
Tsp45I 1742 GTGAC ^GT[GC]AC
XmaCI 12 CCCGGG C^CCGGG
XmaI 12 CCCGGG C^CCGGG
XmnI 994 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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Alw44I 1291 GTGCAC G^TGCAC
1771 GTGCAC
AlwNI 556 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
596 CAGCCACTG
ApaLI 1291 GTGCAC G^TGCAC
1771 GTGCAC
BanI 64 GGCACC G^G[CT][GA]CC
173 GGTGCC
BbvCI 850 CCTCAGC CCTCAGC(-5/-2)
1564 GCTGAGG
BclI 516 TGATCA T^GATCA
2070 TGATCA
BglII 303 AGATCT A^GATCT
429 AGATCT
BpmI 296 CTGGAG CTGGAG(16/14)
1588 CTGGAG
BsgI 1780 GTGCAG GTGCAG(16/14)
2018 GTGCAG
BspHI 261 TCATGA T^CATGA
2172 TCATGA
BsrBI 4 GAGCGG CCGCTC(-3/-3)
55 GAGCGG
BsrDI 280 GCAATG GCAATG(2/0)
1351 GCAATG
BstAPI 666 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1773 GCACACTGTGC
DraIII 1294 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
1774 CACACTGTG
DsaI 626 CCATGG C^C[GA][CT]GG
812 CCATGG
EaeI 22 CGGCCG [CT]^GGCC[GA]
2151 TGGCCA
FbaI 516 TGATCA T^GATCA
2070 TGATCA
HincII 871 GTTGAC GT[CT]^[GA]AC
2226 GTTAAC
HindII 871 GTTGAC GT[CT]^[GA]AC
2226 GTTAAC
NcoI 626 CCATGG C^CATGG
812 CCATGG
NspI 730 GCATGT [GA]CATG^[CT]
1611 GCATGC
PpuMI 845 GGGACCC [GA]G^G[AT]CC[CT]
864 AGGACCT
PstI 553 CTGCAG CTGCA^G
673 CTGCAG
RcaI 261 TCATGA T^CATGA
2172 TCATGA
StuI 224 AGGCCT AGG^CCT
655 AGGCCT
TaqI 396 TCGA T^CGA
426 TCGA
TthHB8I 396 TCGA T^CGA
426 TCGA
XbaI 1116 TCTAGA T^CTAGA
2010 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BamHI BanII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TfiI
Tru9I TseI Tsp509I TspRI XhoII