(Circular) (Six-base) MAPSORT of: pFN21AE2076.seq Check: 6444 from: 1 to: 6940 pFN21AE2076 6940 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 4750 278 Size: 53 83 186 936 2125 1089 2468 Fragments arranged by size: 2468 2125 1089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4283 1937 Size: 2346 4594 Acc65I G'GTAC_C Cuts at: 4261 4261 Size: 6940 AclI AA'CG_TT Cuts at: 1563 5067 5440 1563 Size: 3504 373 3063 Fragments arranged by size: 3504 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 2444 3309 3498 3714 4047 4104 5003 Size: 633 179 865 189 216 333 57 899 Cuts at: 5003 6387 6655 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 899 865 633 333 268 216 189 179 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6743 AflIII A'CryG_T Cuts at: 1203 5839 6816 1203 Size: 4636 977 1327 Fragments arranged by size: 4636 1327 977 AgeI A'CCGG_T Cuts at: 5771 5771 Size: 6940 AhdI GACnn_n'nnGTC Cuts at: 5669 5669 Size: 6940 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6940 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6940 AlwNI CAG_nnn'CTG Cuts at: 2566 2606 4683 6255 2566 Size: 40 2077 1572 3251 Fragments arranged by size: 3251 2077 1572 40 ApaI G_GGCC'C Cuts at: 2046 2046 Size: 6940 ApaLI G'TGCA_C Cuts at: 3296 3776 4997 6153 3296 Size: 480 1221 1156 4083 Fragments arranged by size: 4083 1221 1156 480 ApoI r'AATT_y Cuts at: 1493 2929 3081 4141 4249 4475 4627 6736 1493 Size: 1436 152 1060 108 226 152 2109 1697 Fragments arranged by size: 2109 1697 1436 1060 226 152 152 108 AseI AT'TA_AT Cuts at: 160 1032 2912 5494 160 Size: 872 1880 2582 1606 Fragments arranged by size: 2582 1880 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6940 AvaI C'yCGr_G Cuts at: 1870 1943 2017 4265 1870 Size: 73 74 2248 4545 Fragments arranged by size: 4545 2248 74 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2546 2579 2546 Size: 33 6907 BamHI G'GATC_C Cuts at: 2716 3091 4007 4270 4637 2716 Size: 375 916 263 367 5019 Fragments arranged by size: 5019 916 375 367 263 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2178 4261 5716 618 Size: 325 217 627 282 109 2083 1455 1842 Fragments arranged by size: 2083 1842 1455 627 325 282 217 109 BanII G_rGCy'C Cuts at: 1391 2046 2580 3647 4259 1391 Size: 655 534 1067 612 4072 Fragments arranged by size: 4072 1067 655 612 534 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2996 3327 3359 3661 928 Size: 971 1097 331 32 302 4207 Fragments arranged by size: 4207 1097 971 331 302 32 BbvCI CC'TCA_GC Cuts at: 2856 3570 2856 Size: 714 6226 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5131 5165 1903 Size: 34 3194 34 3678 Fragments arranged by size: 3678 3194 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4832 6042 3581 Size: 1251 1210 4479 Fragments arranged by size: 4479 1251 1210 BclI T'GATC_A Cuts at: 1548 2521 4075 1548 Size: 973 1554 4413 Fragments arranged by size: 4413 1554 973 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2020 5551 136 Size: 107 122 71 631 605 348 3531 1525 Fragments arranged by size: 3531 1525 631 605 348 122 107 71 BglII A'GATC_T Cuts at: 2308 2434 6935 2308 Size: 126 4501 2313 Fragments arranged by size: 4501 2313 126 BlpI GC'TnA_GC Cuts at: 4357 4357 Size: 6940 Bme1580I G_kGCm'C Cuts at: 1790 2046 2072 3300 3780 5001 6157 1790 Size: 256 26 1228 480 1221 1156 2573 Fragments arranged by size: 2573 1228 1221 1156 480 256 26 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6940 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4671 5629 442 Size: 416 53 83 399 24 3254 958 1753 Fragments arranged by size: 3254 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6940 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2327 2327 Size: 6940 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2322 3614 5600 1239 Size: 1083 1292 1986 2579 Fragments arranged by size: 2579 1986 1292 1083 Bpu10I CC'TnA_GC Cuts at: 2856 3570 2856 Size: 714 6226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3042 3791 4089 5065 5930 6228 3042 Size: 749 298 976 865 298 3754 Fragments arranged by size: 3754 976 865 749 298 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5603 882 Size: 617 4104 2219 Fragments arranged by size: 4104 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4731 493 Size: 711 3527 2702 Fragments arranged by size: 3527 2702 711 BsaBI GATnn'nnATC Cuts at: 2520 6934 2520 Size: 4414 2526 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3658 4747 5129 5764 Size: 53 83 186 936 2125 1089 382 635 Cuts at: 5764 275 Size: 1451 Fragments arranged by size: 2125 1451 1089 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5373 5771 6045 6192 6603 5373 Size: 398 274 147 411 5710 Fragments arranged by size: 5710 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2859 2889 1178 Size: 30 165 30 1456 30 5229 Fragments arranged by size: 5229 1456 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2319 2880 4092 1186 Size: 1133 561 1212 4034 Fragments arranged by size: 4034 1212 1133 561 BseYI C'CCAG_C Cuts at: 2065 6143 6639 2065 Size: 4078 496 2366 Fragments arranged by size: 4078 2366 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3806 4044 1163 Size: 573 2070 238 4059 Fragments arranged by size: 4059 2070 573 238 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2030 4390 5151 6179 1540 Size: 275 186 29 2360 761 1028 2301 Fragments arranged by size: 2360 2301 1028 761 275 186 29 BsiHKAI G_wGCw'C Cuts at: 2580 3300 3780 3807 5001 5086 6157 2580 Size: 720 480 27 1194 85 1071 3363 Fragments arranged by size: 3363 1194 1071 720 480 85 27 BsmI GAATG_Cn' Cuts at: 3462 4451 4544 3462 Size: 989 93 5858 Fragments arranged by size: 5858 989 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2072 2580 3300 3647 3780 3807 Size: 399 256 26 508 720 347 133 27 Cuts at: 3807 4259 5001 5086 6157 1391 Size: 452 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 720 508 452 399 347 256 133 85 27 26 BspEI T'CCGG_A Cuts at: 6603 6603 Size: 6940 BspHI T'CATG_A Cuts at: 2266 4177 4829 6559 2266 Size: 1911 652 1730 2647 Fragments arranged by size: 2647 1911 1730 652 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4295 844 Size: 3451 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2011 2062 4827 1394 Size: 429 188 51 2765 3507 Fragments arranged by size: 3507 2765 429 188 51 BsrDI GCAATG_nn' Cuts at: 65 1248 2292 3363 5435 5609 65 Size: 1183 1044 1071 2072 174 1396 Fragments arranged by size: 2072 1396 1183 1071 1044 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 5584 5771 1700 Size: 110 262 3512 187 2869 Fragments arranged by size: 3512 2869 262 187 110 BsrGI T'GTAC_A Cuts at: 96 2537 3235 3977 96 Size: 2441 698 742 3059 Fragments arranged by size: 3059 2441 742 698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6940 BssSI C'ACGA_G Cuts at: 5000 6012 5000 Size: 1012 5928 BstAPI GCAn_nnn'nTGC Cuts at: 794 2677 3784 794 Size: 1883 1107 3950 Fragments arranged by size: 3950 1883 1107 BstBI TT'CG_AA Cuts at: 5744 5744 Size: 6940 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6940 BstXI CCAn_nnnn'nTGG Cuts at: 2691 2691 Size: 6940 BstYI r'GATC_y Cuts at: 1972 2308 2434 2716 3091 4007 4270 4637 5022 Size: 336 126 282 375 916 263 367 385 Cuts at: 5022 5039 6491 6577 6589 6935 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 916 385 375 367 346 336 282 263 126 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3873 3873 Size: 6940 BtgI C'CryG_G Cuts at: 513 1065 2004 2631 2817 513 Size: 552 939 627 186 4636 Fragments arranged by size: 4636 939 627 552 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6201 Fragments arranged by size: 6201 683 56 BtsI GCAGTG_nn' Cuts at: 2555 3300 4456 5250 5270 2555 Size: 745 1156 794 20 4225 Fragments arranged by size: 4225 1156 794 745 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6940 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6905 DraI TTT'AAA Cuts at: 4244 4244 Size: 6940 DraIII CAC_nnn'GTG Cuts at: 3304 3784 3304 Size: 480 6460 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3917 5947 809 Size: 507 2601 2030 1802 Fragments arranged by size: 2601 2030 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 4156 4387 Size: 54 1425 50 275 18 197 2129 231 Cuts at: 4387 5276 6905 8 Size: 889 1629 43 Fragments arranged by size: 2129 1629 1425 889 275 231 197 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2027 4387 1537 Size: 275 215 2360 4090 Fragments arranged by size: 4090 2360 275 215 EarI CTCTTCn'nnn_ Cuts at: 1353 2283 2736 3635 4870 1353 Size: 930 453 899 1235 3423 Fragments arranged by size: 3423 1235 930 899 453 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5527 5901 6047 387 Size: 1064 138 3938 374 146 1280 Fragments arranged by size: 3938 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2578 2578 Size: 6940 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 2322 2444 3309 3498 3614 3714 Size: 393 633 57 122 865 189 116 100 Cuts at: 3714 4047 4104 5003 5600 6387 6655 1239 Size: 333 57 899 597 787 268 1524 Fragments arranged by size: 1524 899 865 787 633 597 393 333 268 189 122 116 100 57 57 EcoO109I rG'GnC_Cy Cuts at: 2043 2851 2870 2043 Size: 808 19 6113 Fragments arranged by size: 6113 808 19 EcoRI G'AATT_C Cuts at: 4249 4627 4249 Size: 378 6562 EcoRV GAT'ATC Cuts at: 3510 3510 Size: 6940 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3083 3083 Size: 6940 FspI TGC'GCA Cuts at: 5446 5446 Size: 6940 HaeII r_GCGC'y Cuts at: 1678 6087 1678 Size: 4409 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 3664 3696 3847 Size: 32 135 32 803 32 1257 32 151 Cuts at: 3847 3879 5581 5613 5655 5687 1373 Size: 32 1702 32 42 32 2626 Fragments arranged by size: 2626 1702 1257 803 151 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2878 4233 4284 4530 669 Size: 988 281 940 1355 51 246 3079 Fragments arranged by size: 3079 1355 988 940 281 246 51 HindIII A'AGCT_T Cuts at: 748 748 Size: 6940 HpaI GTT'AAC Cuts at: 4233 4530 4233 Size: 297 6643 KpnI G_GTAC'C Cuts at: 4265 4265 Size: 6940 MfeI C'AATT_G Cuts at: 4539 4539 Size: 6940 MluI A'CGCG_T Cuts at: 6816 6816 Size: 6940 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6054 6238 1305 Size: 4749 184 2007 Fragments arranged by size: 4749 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4158 10 Size: 54 1425 343 2326 2792 Fragments arranged by size: 2792 2326 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2217 3758 3926 4045 4898 5257 5416 Size: 648 1051 1541 168 119 853 359 159 Cuts at: 5416 518 Size: 2042 Fragments arranged by size: 2042 1541 1051 853 648 359 168 159 119 MspA1I CmG'CkG Cuts at: 1606 3271 4356 5033 6181 6426 1606 Size: 1665 1085 677 1148 245 2120 Fragments arranged by size: 2120 1665 1148 1085 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2631 2817 513 Size: 552 939 627 186 4636 Fragments arranged by size: 4636 939 627 552 186 NdeI CA'TA_TG Cuts at: 387 387 Size: 6940 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6940 NotI GC'GGCC_GC Cuts at: 4387 4387 Size: 6940 NspI r_CATG'y Cuts at: 1132 1229 2739 3620 4298 5843 1132 Size: 97 1510 881 678 1545 2229 Fragments arranged by size: 2229 1545 1510 881 678 97 PasI CC'CwG_GG Cuts at: 1348 2046 1348 Size: 698 6242 PciI A'CATG_T Cuts at: 5839 5839 Size: 6940 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6940 PmeI GTTT'AAAC Cuts at: 4244 4244 Size: 6940 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2103 2135 5009 5041 6522 6554 2103 Size: 32 2874 32 1481 32 2489 Fragments arranged by size: 2874 2489 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2851 2870 2851 Size: 19 6921 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6940 PsiI TTA'TAA Cuts at: 4510 4510 Size: 6940 PspOMI G'GGCC_C Cuts at: 2042 2042 Size: 6940 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3970 4002 3970 Size: 32 6908 PstI C_TGCA'G Cuts at: 830 2562 2682 4292 830 Size: 1732 120 1610 3478 Fragments arranged by size: 3478 1732 1610 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6940 PvuII CAG'CTG Cuts at: 3271 3271 Size: 6940 SacI G_AGCT'C Cuts at: 2580 2580 Size: 6940 SalI G'TCGA_C Cuts at: 1936 4282 1936 Size: 2346 4594 SanDI GG'GwC_CC Cuts at: 2851 2851 Size: 6940 SapI GCTCTTCn'nnn_ Cuts at: 3635 3635 Size: 6940 SbfI CC_TGCA'GG Cuts at: 4292 4292 Size: 6940 ScaI AGT'ACT Cuts at: 5188 6900 5188 Size: 1712 5228 SfcI C'TryA_G Cuts at: 826 1045 2558 2678 2831 3014 3325 3635 3742 Size: 219 1513 120 153 183 311 310 107 Cuts at: 3742 4288 5423 6104 6295 826 Size: 546 1135 681 191 1471 Fragments arranged by size: 1513 1471 1135 681 546 311 310 219 191 183 153 120 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6940 SgrAI Cr'CCGG_yG Cuts at: 1700 2072 1700 Size: 372 6568 SmaI CCC'GGG Cuts at: 1872 2019 4267 1872 Size: 147 2248 4545 Fragments arranged by size: 4545 2248 147 SmlI C'TyrA_G Cuts at: 820 1017 1943 3057 3806 4104 5044 5945 6207 Size: 197 926 1114 749 298 940 901 262 Cuts at: 6207 820 Size: 1553 Fragments arranged by size: 1553 1114 940 926 901 749 298 262 197 SnaBI TAC'GTA Cuts at: 493 4731 493 Size: 4238 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6940 SphI G_CATG'C Cuts at: 1132 3620 4298 1132 Size: 2488 678 3774 Fragments arranged by size: 3774 2488 678 SspI AAT'ATT Cuts at: 5 52 2257 4864 5 Size: 47 2205 2607 2081 Fragments arranged by size: 2607 2205 2081 47 StuI AGG'CCT Cuts at: 1517 2231 2662 1517 Size: 714 431 5795 Fragments arranged by size: 5795 714 431 StyI C'CwwG_G Cuts at: 513 1065 2004 2631 2817 4153 4379 513 Size: 552 939 627 186 1336 226 3074 Fragments arranged by size: 3074 1336 939 627 552 226 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4967 4984 5137 5322 5767 325 Size: 1255 3387 17 153 185 445 1498 Fragments arranged by size: 3387 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2537 2941 3235 Size: 274 80 33 51 1443 560 404 294 Cuts at: 3235 3977 5186 6898 96 Size: 742 1209 1712 138 Fragments arranged by size: 1712 1443 1209 742 560 404 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2708 2740 6900 6932 2708 Size: 32 4160 32 2716 Fragments arranged by size: 4160 2716 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6940 XbaI T'CTAG_A Cuts at: 3121 4015 4276 3121 Size: 894 261 5785 Fragments arranged by size: 5785 894 261 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6940 XmaI C'CCGG_G Cuts at: 1870 2017 4265 1870 Size: 147 2248 4545 Fragments arranged by size: 4545 2248 147 XmnI GAAnn'nnTTC Cuts at: 3003 5069 3003 Size: 2066 4874 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 4748 276 Size: 53 83 186 936 2125 1089 2468 Fragments arranged by size: 2468 2125 1089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI