Restriction map for pFN21AE2076
 (Linear) (Six-base) MAPSORT of: KIEE2076.seq  Check: 6567  from: 1  to: 2235



KIEE2076.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AatII G_ACGT'C

Cuts at:      0    1656    2235 
   Size:       1656     579
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     439    1304    1493    1709    2042    2099    2235 
   Size:        260     179     865     189     216     333      57     136

  Fragments arranged by size: 

                 865     333     260     216     189     179     136      57
            
 
AlwNI CAG_nnn'CTG

Cuts at:      0     561     601    2235 
   Size:        561      40    1634

  Fragments arranged by size: 

                1634     561      40
 
ApaI G_GGCC'C

Cuts at:      0      41    2235 
   Size:         41    2194
 
ApaLI G'TGCA_C

Cuts at:      0    1291    1771    2235 
   Size:       1291     480     464

  Fragments arranged by size: 

                1291     480     464
 
ApoI r'AATT_y

Cuts at:      0     924    1076    2136    2235 
   Size:        924     152    1060      99

  Fragments arranged by size: 

                1060     924     152      99
 
AseI AT'TA_AT

Cuts at:      0     907    2235 
   Size:        907    1328
 
AvaI C'yCGr_G

Cuts at:      0      12    2235 
   Size:         12    2223
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     541     574    2235 
   Size:        541      33    1661

  Fragments arranged by size: 

                1661     541      33
 
BamHI G'GATC_C

Cuts at:      0     711    1086    2002    2235 
   Size:        711     375     916     233

  Fragments arranged by size: 

                 916     711     375     233
 
BanI G'GyrC_C

Cuts at:      0      64     173    2235 
   Size:         64     109    2062

  Fragments arranged by size: 

                2062     109      64
 
BanII G_rGCy'C

Cuts at:      0      41     575    1642    2235 
   Size:         41     534    1067     593

  Fragments arranged by size: 

                1067     593     534      41
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     991    1322    1354    1656    2235 
   Size:        991     331      32     302     579

  Fragments arranged by size: 

                 991     579     331     302      32
 
BbvCI CC'TCA_GC

Cuts at:      0     851    1565    2235 
   Size:        851     714     670

  Fragments arranged by size: 

                 851     714     670
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1576    2235 
   Size:       1576     659
 
BclI T'GATC_A

Cuts at:      0     516    2070    2235 
   Size:        516    1554     165

  Fragments arranged by size: 

                1554     516     165
 
BglI GCCn_nnn'nGGC

Cuts at:      0      15    2235 
   Size:         15    2220
 
BglII A'GATC_T

Cuts at:      0     303     429    2235 
   Size:        303     126    1806

  Fragments arranged by size: 

                1806     303     126
 
Bme1580I G_kGCm'C

Cuts at:      0      41      67    1295    1775    2235 
   Size:         41      26    1228     480     460

  Fragments arranged by size: 

                1228     480     460      41      26
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     322    2235 
   Size:        322    1913
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317    1609    2235 
   Size:        317    1292     626

  Fragments arranged by size: 

                1292     626     317
 
Bpu10I CC'TnA_GC

Cuts at:      0     851    1565    2235 
   Size:        851     714     670

  Fragments arranged by size: 

                 851     714     670
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1037    1786    2084    2235 
   Size:       1037     749     298     151

  Fragments arranged by size: 

                1037     749     298     151
 
BsaBI GATnn'nnATC

Cuts at:      0     515    2235 
   Size:        515    1720
 
BsaHI Gr'CG_yC

Cuts at:      0    1653    2235 
   Size:       1653     582
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     854     884    2235 
   Size:        854      30    1351

  Fragments arranged by size: 

                1351     854      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     314     875    2087    2235 
   Size:        314     561    1212     148

  Fragments arranged by size: 

                1212     561     314     148
 
BseYI C'CCAG_C

Cuts at:      0      60    2235 
   Size:         60    2175
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1801    2039    2235 
   Size:       1801     238     196

  Fragments arranged by size: 

                1801     238     196
 
BsiEI CG_ry'CG

Cuts at:      0      25    2235 
   Size:         25    2210
 
BsiHKAI G_wGCw'C

Cuts at:      0     575    1295    1775    1802    2235 
   Size:        575     720     480      27     433

  Fragments arranged by size: 

                 720     575     480     433      27
 
BsmI GAATG_Cn'

Cuts at:      0    1457    2235 
   Size:       1457     778
 
Bsp1286I G_dGCh'C

Cuts at:      0      41      67     575    1295    1642    1775    1802    2235 
   Size:         41      26     508     720     347     133      27     433

  Fragments arranged by size: 

                 720     508     433     347     133      41      27      26
            
 
BspHI T'CATG_A

Cuts at:      0     261    2172    2235 
   Size:        261    1911      63

  Fragments arranged by size: 

                1911     261      63
 
BsrBI CCG'CTC

Cuts at:      0       6      57    2235 
   Size:          6      51    2178

  Fragments arranged by size: 

                2178      51       6
 
BsrDI GCAATG_nn'

Cuts at:      0     287    1358    2235 
   Size:        287    1071     877

  Fragments arranged by size: 

                1071     877     287
 
BsrFI r'CCGG_y

Cuts at:      0      67    2235 
   Size:         67    2168
 
BsrGI T'GTAC_A

Cuts at:      0     532    1230    1972    2235 
   Size:        532     698     742     263

  Fragments arranged by size: 

                 742     698     532     263
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     672    1779    2235 
   Size:        672    1107     456

  Fragments arranged by size: 

                1107     672     456
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     686    2235 
   Size:        686    1549
 
BstYI r'GATC_y

Cuts at:      0     303     429     711    1086    2002    2235 
   Size:        303     126     282     375     916     233

  Fragments arranged by size: 

                 916     375     303     282     233     126
 
Bsu36I CC'TnA_GG

Cuts at:      0    1868    2235 
   Size:       1868     367
 
BtgI C'CryG_G

Cuts at:      0     626     812    2235 
   Size:        626     186    1423

  Fragments arranged by size: 

                1423     626     186
 
BtsI GCAGTG_nn'

Cuts at:      0     550    1295    2235 
   Size:        550     745     940

  Fragments arranged by size: 

                 940     745     550
 
DraIII CAC_nnn'GTG

Cuts at:      0    1299    1779    2235 
   Size:       1299     480     456

  Fragments arranged by size: 

                1299     480     456
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1912    2235 
   Size:       1912     323
 
EaeI y'GGCC_r

Cuts at:      0      22    2151    2235 
   Size:         22    2129      84

  Fragments arranged by size: 

                2129      84      22
 
EagI C'GGCC_G

Cuts at:      0      22    2235 
   Size:         22    2213
 
EarI CTCTTCn'nnn_

Cuts at:      0     278     731    1630    2235 
   Size:        278     453     899     605

  Fragments arranged by size: 

                 899     605     453     278
 
EcoICRI GAG'CTC

Cuts at:      0     573    2235 
   Size:        573    1662
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     317     439    1304    1493    1609    1709    2042 
   Size:        260      57     122     865     189     116     100     333

Cuts at:   2042    2099    2235 
   Size:         57     136

  Fragments arranged by size: 

                 865     333     260     189     136     122     116     100
                  57      57
 
EcoO109I rG'GnC_Cy

Cuts at:      0      38     846     865    2235 
   Size:         38     808      19    1370

  Fragments arranged by size: 

                1370     808      38      19
 
EcoRV GAT'ATC

Cuts at:      0    1505    2235 
   Size:       1505     730
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1078    2235 
   Size:       1078    1157
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     370     402    1659    1691    1842    1874    2235 
   Size:        370      32    1257      32     151      32     361

  Fragments arranged by size: 

                1257     370     361     151      32      32      32
 
HincII GTy'rAC

Cuts at:      0     873    2228    2235 
   Size:        873    1355       7

  Fragments arranged by size: 

                1355     873       7
 
HpaI GTT'AAC

Cuts at:      0    2228    2235 
   Size:       2228       7
 
MscI TGG'CCA

Cuts at:      0    2153    2235 
   Size:       2153      82
 
MslI CAynn'nnrTG

Cuts at:      0     212    1753    1921    2040    2235 
   Size:        212    1541     168     119     195

  Fragments arranged by size: 

                1541     212     195     168     119
 
MspA1I CmG'CkG

Cuts at:      0    1266    2235 
   Size:       1266     969
 
NcoI C'CATG_G

Cuts at:      0     626     812    2235 
   Size:        626     186    1423

  Fragments arranged by size: 

                1423     626     186
 
NspI r_CATG'y

Cuts at:      0     734    1615    2235 
   Size:        734     881     620

  Fragments arranged by size: 

                 881     734     620
 
PasI CC'CwG_GG

Cuts at:      0      41    2235 
   Size:         41    2194
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      98     130    2235 
   Size:         98      32    2105

  Fragments arranged by size: 

                2105      98      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     846     865    2235 
   Size:        846      19    1370

  Fragments arranged by size: 

                1370     846      19
 
PspOMI G'GGCC_C

Cuts at:      0      37    2235 
   Size:         37    2198
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1965    1997    2235 
   Size:       1965      32     238

  Fragments arranged by size: 

                1965     238      32
 
PstI C_TGCA'G

Cuts at:      0     557     677    2235 
   Size:        557     120    1558

  Fragments arranged by size: 

                1558     557     120
 
PvuII CAG'CTG

Cuts at:      0    1266    2235 
   Size:       1266     969
 
SacI G_AGCT'C

Cuts at:      0     575    2235 
   Size:        575    1660
 
SanDI GG'GwC_CC

Cuts at:      0     846    2235 
   Size:        846    1389
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1630    2235 
   Size:       1630     605
 
SfcI C'TryA_G

Cuts at:      0     553     673     826    1009    1320    1630    1737    2235 
   Size:        553     120     153     183     311     310     107     498

  Fragments arranged by size: 

                 553     498     311     310     183     153     120     107
            
 
SgrAI Cr'CCGG_yG

Cuts at:      0      67    2235 
   Size:         67    2168
 
SmaI CCC'GGG

Cuts at:      0      14    2235 
   Size:         14    2221
 
SmlI C'TyrA_G

Cuts at:      0    1052    1801    2099    2235 
   Size:       1052     749     298     136

  Fragments arranged by size: 

                1052     749     298     136
 
SphI G_CATG'C

Cuts at:      0    1615    2235 
   Size:       1615     620
 
SspI AAT'ATT

Cuts at:      0     252    2235 
   Size:        252    1983
 
StuI AGG'CCT

Cuts at:      0     226     657    2235 
   Size:        226     431    1578

  Fragments arranged by size: 

                1578     431     226
 
StyI C'CwwG_G

Cuts at:      0     626     812    2148    2235 
   Size:        626     186    1336      87

  Fragments arranged by size: 

                1336     626     186      87
 
TatI w'GTAC_w

Cuts at:      0     532     936    1230    1972    2235 
   Size:        532     404     294     742     263

  Fragments arranged by size: 

                 742     532     404     294     263
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     703     735    2235 
   Size:        703      32    1500

  Fragments arranged by size: 

                1500     703      32
 
XbaI T'CTAG_A

Cuts at:      0    1116    2010    2235 
   Size:       1116     894     225

  Fragments arranged by size: 

                1116     894     225
 
XmaI C'CCGG_G

Cuts at:      0      12    2235 
   Size:         12    2223
 
XmnI GAAnn'nnTTC

Cuts at:      0     998    2235 
   Size:        998    1237
 
ZraI GAC'GTC

Cuts at:      0    1654    2235 
   Size:       1654     581
 

 Enzymes that do cut:

    AatII     AcuI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI
     BaeI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI
     BglI    BglII Bme1580I     BplI     BpmI   Bpu10I    BpuEI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI     EaeI     EagI
     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I   HincII
     HpaI     MscI     MslI   MspA1I     NcoI     NspI     PasI     PpiI
    PpuMI   PspOMI     PsrI     PstI    PvuII     SacI    SanDI     SapI
     SfcI    SgrAI     SmaI     SmlI     SphI     SspI     StuI     StyI
     TatI     TstI     XbaI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     AscI    AsiSI    AvrII     BbeI
     BcgI    BfrBI     BlpI    BmgBI     BmrI     BmtI     BsaI    BsaAI
    BsaWI    BsiWI    BsmBI    BspEI    BspMI   BssHII    BssSI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI     EciI    EcoNI
    EcoRI     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI    PshAI     PsiI    PspXI     PvuI    RsrII    SacII     SalI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SnaBI     SpeI
     SrfI     SwaI    TaqII  Tth111I     XcmI     XhoI
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