################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbvCI BcgI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BspDI BspEI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 1456 CGCG CG^CG AcsI 310 GAATTT [GA]^AATT[CT] AcyI 1942 GACGCC G[GA]^CG[CT]C AflIII 1907 ACATGT A^C[GA][CT]GT AocI 1818 CCTCAGG CC^T[ACGT]AGG ApaI 1466 GGGCCC GGGCC^C ApoI 310 GAATTT [GA]^AATT[CT] AvaI 297 CCCGGG C^[CT]CG[GA]G BalI 2006 TGGCCA TGG^CCA BanI 21 GGTGCC G^G[CT][GA]CC BbrPI 1086 CACGTG CAC^GTG BclI 140 TGATCA T^GATCA BglI 527 GCCGTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 1942 GACGCC G[GA]^CG[CT]C BsiEI 1479 CGACCG CG[GA][CT]^CG BsoBI 297 CCCGGG C^[CT]CG[GA]G BspLU11I 1907 ACATGT A^CATGT BsrBI 679 GAGCGG CCGCTC(-3/-3) BsrFI 24 GCCGGC [GA]^CCGG[CT] BstAPI 2030 GCATCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1456 CGCG CG^CG Bsu36I 1818 CCTCAGG CC^T[ACGT]AGG Cfr10I 24 GCCGGC [GA]^CCGG[CT] EaeI 2006 TGGCCA [CT]^GGCC[GA] EarI 1579 GAAGAG CTCTTC(1/4) Eco81I 1818 CCTCAGG CC^T[ACGT]AGG EcoNI 199 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 140 TGATCA T^GATCA Hin1I 1942 GACGCC G[GA]^CG[CT]C Ksp632I 1579 GAAGAG CTCTTC(1/4) MluNI 2006 TGGCCA TGG^CCA MscI 2006 TGGCCA TGG^CCA MvnI 1456 CGCG CG^CG NaeI 24 GCCGGC GCC^GGC NgoMIV 24 GCCGGC G^CCGGC NspI 1907 ACATGT [GA]CATG^[CT] PmaCI 1086 CACGTG CAC^GTG PmlI 1086 CACGTG CAC^GTG PshAI 1633 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1582 GAGCTC GAGCT^C SapI 1579 GAAGAGC GCTCTTC(1/4) ScaI 1208 AGTACT AGT^ACT SexAI 1043 ACCAGGT A^CC[AT]GGT SmaI 297 CCCGGG CCC^GGG SmlI 700 CTCAAG C^T[CT][GA]AG StuI 1853 AGGCCT AGG^CCT TaqI 827 TCGA T^CGA TfiI 1329 GATTC G^A[AT]TC TthHB8I 827 TCGA T^CGA XcmI 1950 CCAGGGGGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 297 CCCGGG C^CCGGG XmaI 297 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspHI 1582 GAGCTC G[AT]GC[AT]^C 2068 GTGCTC BamHI 465 GGATCC G^GATCC 1487 GGATCC BanII 1466 GGGCCC G[GA]GC[CT]^C 1582 GAGCTC BbsI 550 GAAGAC GAAGAC(2/6) 175 GTCTTC BfaI 943 CTAG C^TAG 1594 CTAG BglII 230 AGATCT A^GATCT 767 AGATCT BpuAI 550 GAAGAC GAAGAC(2/6) 175 GTCTTC BsaAI 1086 CACGTG [CT]AC^GT[GA] 2077 TACGTG BsiHKAI 1582 GAGCTC G[AT]GC[AT]^C 2068 GTGCTC BsmI 558 GCATTC GAATGC(1/-1) 1053 GCATTC Bsp1407I 590 TGTACA T^GTACA 1277 TGTACA BspHI 1586 TCATGA T^CATGA 1812 TCATGA BsrDI 1002 CATTGC GCAATG(2/0) 839 GCAATG BsrGI 590 TGTACA T^GTACA 1277 TGTACA BstXI 1074 CCAGGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1169 CCACCACAATGG EcoT14I 321 CCAAGG C^C[AT][AT]GG 1517 CCAAGG HgaI 1942 GACGC GACGC(5/10) 1622 GCGTC MaeI 943 CTAG C^TAG 1594 CTAG PflMI 414 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1345 CCAGTGTGTGG PpuMI 1707 GGGTCCC [GA]G^G[AT]CC[CT] 1982 GGGACCC PstI 36 CTGCAG CTGCA^G 970 CTGCAG PvuII 1064 CAGCTG CAG^CTG 1496 CAGCTG RcaI 1586 TCATGA T^CATGA 1812 TCATGA SspBI 590 TGTACA T^GTACA 1277 TGTACA StyI 321 CCAAGG C^C[AT][AT]GG 1517 CCAAGG Tsp509I 311 AATT ^AATT 1657 AATT Van91I 414 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1345 CCAGTGTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BbvI BciVI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI XhoII