(Circular) (Six-base) MAPSORT of: pFN21AE2060.seq Check: 1976 from: 1 to: 6820 pFN21AE2060 6820 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3296 3427 3296 Size: 131 6689 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4630 278 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4163 1937 Size: 2226 4594 Acc65I G'GTAC_C Cuts at: 4141 4141 Size: 6820 AclI AA'CG_TT Cuts at: 1563 4947 5320 1563 Size: 3384 373 3063 Fragments arranged by size: 3384 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 2534 4883 6267 6535 1632 Size: 597 305 2349 1384 268 1917 Fragments arranged by size: 2349 1917 1384 597 305 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6623 AflIII A'CryG_T Cuts at: 1203 3912 5719 6696 1203 Size: 2709 1807 977 1327 Fragments arranged by size: 2709 1807 1327 977 AgeI A'CCGG_T Cuts at: 5651 5651 Size: 6820 AhdI GACnn_n'nnGTC Cuts at: 5549 5549 Size: 6820 AleI CACnn'nnGTG Cuts at: 1166 3578 4070 1166 Size: 2412 492 3916 Fragments arranged by size: 3916 2412 492 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6820 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2846 2878 2846 Size: 32 6788 AlwNI CAG_nnn'CTG Cuts at: 2494 2974 3055 4563 6135 2494 Size: 480 81 1508 1572 3179 Fragments arranged by size: 3179 1572 1508 480 81 ApaI G_GGCC'C Cuts at: 3475 3475 Size: 6820 ApaLI G'TGCA_C Cuts at: 4877 6033 4877 Size: 1156 5664 ApoI r'AATT_y Cuts at: 1493 2315 4129 4355 4507 6616 1493 Size: 822 1814 226 152 2109 1697 Fragments arranged by size: 2109 1814 1697 822 226 152 AseI AT'TA_AT Cuts at: 160 1032 5374 160 Size: 872 4342 1606 Fragments arranged by size: 4342 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 AvaI C'yCGr_G Cuts at: 1870 1943 2302 4145 1870 Size: 73 359 1843 4545 Fragments arranged by size: 4545 1843 359 73 BamHI G'GATC_C Cuts at: 2470 3492 4150 4517 2470 Size: 1022 658 367 4773 Fragments arranged by size: 4773 1022 658 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 4141 5596 618 Size: 325 217 627 239 2115 1455 1842 Fragments arranged by size: 2115 1842 1455 627 325 239 217 BanII G_rGCy'C Cuts at: 1391 3475 3591 4139 1391 Size: 2084 116 548 4072 Fragments arranged by size: 4072 2084 548 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2173 2562 928 Size: 971 274 389 5186 Fragments arranged by size: 5186 971 389 274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5011 5045 1903 Size: 34 3074 34 3678 Fragments arranged by size: 3678 3074 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2156 3218 3528 4712 5922 2156 Size: 1062 310 1184 1210 3054 Fragments arranged by size: 3054 1210 1184 1062 310 BclI T'GATC_A Cuts at: 1548 2145 1548 Size: 597 6223 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2538 5431 136 Size: 107 122 71 631 605 866 2893 1525 Fragments arranged by size: 2893 1525 866 631 605 122 107 71 BglII A'GATC_T Cuts at: 2235 2772 6815 2235 Size: 537 4043 2240 Fragments arranged by size: 4043 2240 537 BlpI GC'TnA_GC Cuts at: 4237 4237 Size: 6820 Bme1580I G_kGCm'C Cuts at: 1790 2933 3475 4881 6037 1790 Size: 1143 542 1406 1156 2573 Fragments arranged by size: 2573 1406 1156 1143 542 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6820 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2288 2300 2476 Size: 416 53 83 399 24 871 12 176 Cuts at: 2476 4551 5509 442 Size: 2075 958 1753 Fragments arranged by size: 2075 1753 958 871 416 399 176 83 53 24 12 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6820 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2605 2648 2735 3707 5480 1239 Size: 980 386 43 87 972 1773 2579 Fragments arranged by size: 2579 1773 980 972 386 87 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2690 4945 5810 6108 2690 Size: 2255 865 298 3402 Fragments arranged by size: 3402 2255 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5483 882 Size: 617 3984 2219 Fragments arranged by size: 3984 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3093 4084 4611 493 Size: 711 1889 991 527 2702 Fragments arranged by size: 2702 1889 991 711 527 BsaBI GATnn'nnATC Cuts at: 6814 6814 Size: 6820 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3948 4627 5009 5644 Size: 53 83 186 936 2415 679 382 635 Cuts at: 5644 275 Size: 1451 Fragments arranged by size: 2415 1451 936 679 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5253 5651 5925 6072 6483 5253 Size: 398 274 147 411 5590 Fragments arranged by size: 5590 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2254 2284 1178 Size: 30 165 30 851 30 5714 Fragments arranged by size: 5714 851 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2387 2480 3713 3818 4124 4127 1186 Size: 1201 93 1233 105 306 3 3879 Fragments arranged by size: 3879 1233 1201 306 105 93 3 BseYI C'CCAG_C Cuts at: 2475 2934 3478 4005 6023 6519 2475 Size: 459 544 527 2018 496 2776 Fragments arranged by size: 2776 2018 544 527 496 459 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2602 2684 3274 4063 1163 Size: 573 866 82 590 789 3920 Fragments arranged by size: 3920 866 789 590 573 82 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3487 4270 5031 6059 1540 Size: 275 186 1486 783 761 1028 2301 Fragments arranged by size: 2301 1486 1028 783 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3591 4077 4881 4966 6037 3591 Size: 486 804 85 1071 4374 Fragments arranged by size: 4374 1071 804 486 85 BsmI GAATG_Cn' Cuts at: 2563 3058 4331 4424 2563 Size: 495 1273 93 4959 Fragments arranged by size: 4959 1273 495 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2933 3475 3591 4077 4139 4881 4966 Size: 399 1143 542 116 486 62 742 85 Cuts at: 4966 6037 1391 Size: 1071 2174 Fragments arranged by size: 2174 1143 1071 742 542 486 399 116 85 62 BspEI T'CCGG_A Cuts at: 6483 6483 Size: 6820 BspHI T'CATG_A Cuts at: 3591 3817 4709 6439 3591 Size: 226 892 1730 3972 Fragments arranged by size: 3972 1730 892 226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2664 2982 3023 3156 3296 3427 4175 844 Size: 1820 318 41 133 140 131 748 3489 Fragments arranged by size: 3489 1820 748 318 140 133 131 41 BsrBI CCG'CTC Cuts at: 1394 1823 2686 4707 1394 Size: 429 863 2021 3507 Fragments arranged by size: 3507 2021 863 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2851 3006 5315 5489 65 Size: 1183 1603 155 2309 174 1396 Fragments arranged by size: 2309 1603 1396 1183 174 155 BsrFI r'CCGG_y Cuts at: 1700 1810 2029 5464 5651 1700 Size: 110 219 3435 187 2869 Fragments arranged by size: 3435 2869 219 187 110 BsrGI T'GTAC_A Cuts at: 96 2595 3282 96 Size: 2499 687 3634 Fragments arranged by size: 3634 2499 687 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6820 BssSI C'ACGA_G Cuts at: 4880 5892 4880 Size: 1012 5808 BstAPI GCAn_nnn'nTGC Cuts at: 794 4041 794 Size: 3247 3573 BstBI TT'CG_AA Cuts at: 5624 5624 Size: 6820 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6820 BstXI CCAn_nnnn'nTGG Cuts at: 3086 3181 3086 Size: 95 6725 BstYI r'GATC_y Cuts at: 1972 2235 2470 2772 2854 3492 4150 4517 4902 Size: 263 235 302 82 638 658 367 385 Cuts at: 4902 4919 6371 6457 6469 6815 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 658 638 385 367 346 302 263 235 86 82 17 12 Bsu36I CC'TnA_GG Cuts at: 3824 3824 Size: 6820 BtgI C'CryG_G Cuts at: 513 1065 2157 3375 3454 4014 513 Size: 552 1092 1218 79 560 3319 Fragments arranged by size: 3319 1218 1092 560 552 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3661 487 Size: 683 56 2435 3646 Fragments arranged by size: 3646 2435 683 56 BtsI GCAGTG_nn' Cuts at: 2749 4336 5130 5150 2749 Size: 1587 794 20 4419 Fragments arranged by size: 4419 1587 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6820 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3112 3147 529 Size: 35 2548 35 4202 Fragments arranged by size: 4202 2548 35 35 DraI TTT'AAA Cuts at: 4124 4124 Size: 6820 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5827 809 Size: 507 4511 1802 Fragments arranged by size: 4511 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4011 4267 5156 Size: 54 1425 50 275 18 2181 256 889 Cuts at: 5156 6785 8 Size: 1629 43 Fragments arranged by size: 2181 1629 1425 889 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4267 1537 Size: 275 2455 4090 Fragments arranged by size: 4090 2455 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3579 4750 1353 Size: 2226 1171 3423 Fragments arranged by size: 3423 2226 1171 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5407 5781 5927 387 Size: 1064 138 3818 374 146 1280 Fragments arranged by size: 3818 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3589 3589 Size: 6820 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2229 2534 2605 2648 2735 3707 Size: 393 587 10 305 71 43 87 972 Cuts at: 3707 4883 5480 6267 6535 1239 Size: 1176 597 787 268 1524 Fragments arranged by size: 1524 1176 972 787 597 587 393 305 268 87 71 43 10 EcoNI CCTnn'n_nnAGG Cuts at: 2208 2208 Size: 6820 EcoO109I rG'GnC_Cy Cuts at: 2689 2820 3471 3472 3713 3988 2689 Size: 131 651 1 241 275 5521 Fragments arranged by size: 5521 651 275 241 131 1 EcoRI G'AATT_C Cuts at: 4129 4507 4129 Size: 378 6442 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2197 2197 Size: 6820 FspI TGC'GCA Cuts at: 5326 5326 Size: 6820 HaeII r_GCGC'y Cuts at: 1678 5967 1678 Size: 4289 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2083 2115 3105 3137 3887 Size: 32 135 32 511 32 990 32 750 Cuts at: 3887 3919 5461 5493 5535 5567 1373 Size: 32 1542 32 42 32 2626 Fragments arranged by size: 2626 1542 990 750 511 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4164 4410 669 Size: 988 281 2226 246 3079 Fragments arranged by size: 3079 2226 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6820 HpaI GTT'AAC Cuts at: 4410 4410 Size: 6820 KpnI G_GTAC'C Cuts at: 4145 4145 Size: 6820 MfeI C'AATT_G Cuts at: 4419 4419 Size: 6820 MluI A'CGCG_T Cuts at: 6696 6696 Size: 6820 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2391 2491 5934 6118 1305 Size: 1086 100 3443 184 2007 Fragments arranged by size: 3443 2007 1086 184 100 MscI TGG'CCA Cuts at: 10 64 1489 1832 4013 10 Size: 54 1425 343 2181 2817 Fragments arranged by size: 2817 2181 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2678 3179 3576 3578 4070 4778 5137 Size: 648 1512 501 397 2 492 708 359 Cuts at: 5137 5296 518 Size: 159 2042 Fragments arranged by size: 2042 1512 708 648 501 492 397 359 159 2 MspA1I CmG'CkG Cuts at: 1606 3071 3489 3503 4236 4913 6061 6306 1606 Size: 1465 418 14 733 677 1148 245 2120 Fragments arranged by size: 2120 1465 1148 733 677 418 245 14 NaeI GCC'GGC Cuts at: 2031 2031 Size: 6820 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6268 NdeI CA'TA_TG Cuts at: 387 387 Size: 6820 NgoMIV G'CCGG_C Cuts at: 2029 2029 Size: 6820 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6820 NotI GC'GGCC_GC Cuts at: 4267 4267 Size: 6820 NspI r_CATG'y Cuts at: 1132 1229 3916 4178 5723 1132 Size: 97 2687 262 1545 2229 Fragments arranged by size: 2687 2229 1545 262 97 PasI CC'CwG_GG Cuts at: 1348 3709 1348 Size: 2361 4459 PciI A'CATG_T Cuts at: 3912 5719 3912 Size: 1807 5013 PflMI CCAn_nnn'nTGG Cuts at: 1108 2425 3356 1108 Size: 1317 931 4572 Fragments arranged by size: 4572 1317 931 PfoI T'CCnGG_A Cuts at: 2447 2712 2447 Size: 265 6555 PmeI GTTT'AAAC Cuts at: 4124 4124 Size: 6820 PmlI CAC'GTG Cuts at: 3093 3093 Size: 6820 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4889 4921 6402 6434 4889 Size: 32 1481 32 5275 Fragments arranged by size: 5275 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3713 3988 3713 Size: 275 6545 PshAI GACnn'nnGTC Cuts at: 1613 3642 1613 Size: 2029 4791 PsiI TTA'TAA Cuts at: 4390 4390 Size: 6820 PspOMI G'GGCC_C Cuts at: 3471 3471 Size: 6820 PstI C_TGCA'G Cuts at: 830 2045 2979 4172 830 Size: 1215 934 1193 3478 Fragments arranged by size: 3478 1215 1193 934 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6820 PvuII CAG'CTG Cuts at: 3071 3503 3071 Size: 432 6388 SacI G_AGCT'C Cuts at: 3591 3591 Size: 6820 SalI G'TCGA_C Cuts at: 1936 4162 1936 Size: 2226 4594 SanDI GG'GwC_CC Cuts at: 3713 3988 3713 Size: 275 6545 SapI GCTCTTCn'nnn_ Cuts at: 3579 3579 Size: 6820 SbfI CC_TGCA'GG Cuts at: 4172 4172 Size: 6820 ScaI AGT'ACT Cuts at: 3215 5068 6780 3215 Size: 1853 1712 3255 Fragments arranged by size: 3255 1853 1712 SexAI A'CCwGG_T Cuts at: 3048 3048 Size: 6820 SfcI C'TryA_G Cuts at: 826 1045 2041 2668 2768 2975 4168 5303 5984 Size: 219 996 627 100 207 1193 1135 681 Cuts at: 5984 6175 826 Size: 191 1471 Fragments arranged by size: 1471 1193 1135 996 681 627 219 207 191 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6820 SmaI CCC'GGG Cuts at: 1872 2304 4147 1872 Size: 432 1843 4545 Fragments arranged by size: 4545 1843 432 SmlI C'TyrA_G Cuts at: 820 1017 1943 2705 4924 5825 6087 820 Size: 197 926 762 2219 901 262 1553 Fragments arranged by size: 2219 1553 926 901 762 262 197 SnaBI TAC'GTA Cuts at: 493 4611 493 Size: 4118 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6820 SphI G_CATG'C Cuts at: 1132 4178 1132 Size: 3046 3774 SspI AAT'ATT Cuts at: 5 52 4744 5 Size: 47 4692 2081 Fragments arranged by size: 4692 2081 47 StuI AGG'CCT Cuts at: 1517 3860 1517 Size: 2343 4477 StyI C'CwwG_G Cuts at: 513 1065 2326 3522 4259 513 Size: 552 1261 1196 737 3074 Fragments arranged by size: 3074 1261 1196 737 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2628 3945 3997 4847 4864 5017 5202 Size: 1255 1048 1317 52 850 17 153 185 Cuts at: 5202 5647 325 Size: 445 1498 Fragments arranged by size: 1498 1317 1255 1048 850 445 185 153 52 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 2746 3213 Size: 274 80 33 51 1443 618 151 467 Cuts at: 3213 3282 5066 6778 96 Size: 69 1784 1712 138 Fragments arranged by size: 1784 1712 1443 618 467 274 151 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2154 2186 2254 2286 3502 3534 3697 3729 6780 Size: 32 68 32 1216 32 163 32 3051 Cuts at: 6780 6812 2154 Size: 32 2162 Fragments arranged by size: 3051 2162 1216 163 68 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6820 XbaI T'CTAG_A Cuts at: 4156 4156 Size: 6820 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3962 3962 Size: 6820 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6820 XmaI C'CCGG_G Cuts at: 1870 2302 4145 1870 Size: 432 1843 4545 Fragments arranged by size: 4545 1843 432 XmnI GAAnn'nnTTC Cuts at: 4949 4949 Size: 6820 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4628 276 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI