Restriction map for
pFN21AE2012
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AlwNI Aor51HI ApoI
AscI AseI AsnI Asp718I AspI
AviII AvrII BbeI BbrPI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BsaAI BsaBI BseAI BsiWI
BsmBI BspDI BspEI BspHI BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 13 GACGCC G[GA]^CG[CT]C
AflIII 1307 ACATGT A^C[GA][CT]GT
AhdI 28 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 298 CCTCAGG CC^T[ACGT]AGG
AspEI 28 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1249 GGTCC G^G[AT]CC
BalI 1547 TGGCCA TGG^CCA
BamHI 1072 GGATCC G^GATCC
BcgI 1433 GCAGCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 16 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 379 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 379 GCTGAGC GC^T[ACGT]AGC
BsaHI 13 GACGCC G[GA]^CG[CT]C
BsaI 82 GAGACC GGTCTC(1/5)
BsaWI 1196 ACCGGA [AT]^CCGG[AT]
BsgI 1047 CTGCAC GTGCAG(16/14)
BsmI 1408 GAATGC GAATGC(1/-1)
Bsp1407I 462 TGTACA T^GTACA
BspLU11I 1307 ACATGT A^CATGT
BspMI 999 GCAGGT ACCTGC(4/8)
BsrFI 1525 ACCGGC [GA]^CCGG[CT]
BsrGI 462 TGTACA T^GTACA
BstAPI 1049 GCACCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 788 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1072 GGATCC [GA]^GATC[CT]
Bsu36I 298 CCTCAGG CC^T[ACGT]AGG
CelII 379 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1525 ACCGGC [GA]^CCGG[CT]
EagI 1527 CGGCCG C^GGCCG
Eam1105I 28 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1527 CGGCCG C^GGCCG
Eco52I 1527 CGGCCG C^GGCCG
Eco81I 298 CCTCAGG CC^T[ACGT]AGG
Hin1I 13 GACGCC G[GA]^CG[CT]C
MflI 1072 GGATCC [GA]^GATC[CT]
MluNI 1547 TGGCCA TGG^CCA
MscI 1547 TGGCCA TGG^CCA
SacI 675 GAGCTC GAGCT^C
SapI 1662 GCTCTTC GCTCTTC(1/4)
SphI 905 GCATGC GCATG^C
SspBI 462 TGTACA T^GTACA
StuI 1280 AGGCCT AGG^CCT
TfiI 224 GATTC G^A[AT]TC
XcmI 785 CCACCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1072 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 36 GTCTAC GT^[AC][GT]AC
349 GTCTAC
AfaI 261 GTAC GT^AC
463 GTAC
Alw44I 1226 GTGCAC G^TGCAC
1471 GTGCAC
AlwI 1073 GATCC GGATC(4/5)
1072 GGATC
ApaLI 1226 GTGCAC G^TGCAC
1471 GTGCAC
Asp700I 1080 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1647 GAACTGCTTC
BbsI 846 GAAGAC GAAGAC(2/6)
1020 GAAGAC
BpuAI 846 GAAGAC GAAGAC(2/6)
1020 GAAGAC
BsiEI 435 CGACCG CG[GA][CT]^CG
1527 CGGCCG
BsrBI 1090 GAGCGG CCGCTC(-3/-3)
1483 GAGCGG
BssSI 1264 CACGAG CACGAG(-5/-1)
1480 CACGAG
DpnII 1068 GATC ^GATC
1073 GATC
DraIII 1229 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1374 CACGCTGTG
DsaI 1013 CCATGG C^C[GA][CT]GG
1544 CCATGG
EaeI 1527 CGGCCG [CT]^GGCC[GA]
1547 TGGCCA
EarI 1132 CTCTTC CTCTTC(1/4)
1663 CTCTTC
HgaI 13 GACGC GACGC(5/10)
740 GCGTC
Ksp632I 1132 CTCTTC CTCTTC(1/4)
1663 CTCTTC
MboI 1068 GATC ^GATC
1073 GATC
NcoI 1013 CCATGG C^CATGG
1544 CCATGG
NdeII 1068 GATC ^GATC
1073 GATC
NspI 905 GCATGC [GA]CATG^[CT]
1307 ACATGT
PstI 488 CTGCAG CTGCA^G
919 CTGCAG
PvuII 377 CAGCTG CAG^CTG
895 CAGCTG
RsaI 261 GTAC GT^AC
463 GTAC
Sau3AI 1068 GATC ^GATC
1073 GATC
SmaI 886 CCCGGG CCC^GGG
1337 CCCGGG
XmaCI 886 CCCGGG C^CCGGG
1337 CCCGGG
XmaI 886 CCCGGG C^CCGGG
1337 CCCGGG
XmnI 1080 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1647 GAACTGCTTC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI ApaI AspHI
AvaI BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I