(Circular) (Six-base) MAPSORT of: pFN21AE2012.seq Check: 4494 from: 1 to: 6376 pFN21AE2012 6376 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2042 2355 3719 1937 Size: 105 313 1364 4594 Fragments arranged by size: 4594 1364 313 105 Acc65I G'GTAC_C Cuts at: 3697 3697 Size: 6376 AclI AA'CG_TT Cuts at: 1563 4503 4876 1563 Size: 2940 373 3063 Fragments arranged by size: 3063 2940 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4439 5823 6091 1632 Size: 2807 1384 268 1917 Fragments arranged by size: 2807 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6179 AflIII A'CryG_T Cuts at: 1203 3312 5275 6252 1203 Size: 2109 1963 977 1327 Fragments arranged by size: 2109 1963 1327 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 2038 5105 2038 Size: 3067 3309 AleI CACnn'nnGTG Cuts at: 1166 3325 1166 Size: 2159 4217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6376 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2819 2851 2819 Size: 32 6344 AlwNI CAG_nnn'CTG Cuts at: 4119 5691 4119 Size: 1572 4804 ApaI G_GGCC'C Cuts at: 2533 2592 2883 2533 Size: 59 291 6026 Fragments arranged by size: 6026 291 59 ApaLI G'TGCA_C Cuts at: 3231 3476 4433 5589 3231 Size: 245 957 1156 4018 Fragments arranged by size: 4018 1156 957 245 ApoI r'AATT_y Cuts at: 1493 3685 3911 4063 6172 1493 Size: 2192 226 152 2109 1697 Fragments arranged by size: 2192 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4930 160 Size: 872 3898 1606 Fragments arranged by size: 3898 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 2331 2891 3038 3342 3569 3701 1870 Size: 73 388 560 147 304 227 132 4545 Fragments arranged by size: 4545 560 388 304 227 147 132 73 BamHI G'GATC_C Cuts at: 3077 3706 4073 3077 Size: 629 367 5380 Fragments arranged by size: 5380 629 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3033 3210 3614 3697 5152 Size: 325 217 627 1246 177 404 83 1455 Cuts at: 5152 618 Size: 1842 Fragments arranged by size: 1842 1455 1246 627 404 325 217 177 83 BanII G_rGCy'C Cuts at: 1391 2533 2540 2592 2684 2883 3695 1391 Size: 1142 7 52 92 199 812 4072 Fragments arranged by size: 4072 1142 812 199 92 52 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2858 3032 928 Size: 971 959 174 4272 Fragments arranged by size: 4272 971 959 174 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3427 3461 4567 4601 1903 Size: 34 1490 34 1106 34 3678 Fragments arranged by size: 3678 1490 1106 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4268 5478 4268 Size: 1210 5166 BclI T'GATC_A Cuts at: 1548 1548 Size: 6376 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 4987 136 Size: 107 122 71 631 605 355 2960 1525 Fragments arranged by size: 2960 1525 631 605 355 122 107 71 BglII A'GATC_T Cuts at: 6371 6371 Size: 6376 BlpI GC'TnA_GC Cuts at: 2385 3793 2385 Size: 1408 4968 Bme1580I G_kGCm'C Cuts at: 1790 2533 2592 2691 2883 3235 3480 3617 4437 Size: 743 59 99 192 352 245 137 820 Cuts at: 4437 5593 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 820 743 352 245 192 137 99 59 BmgBI CAC'GTC Cuts at: 1318 2353 2412 2641 3030 3207 1318 Size: 1035 59 229 389 177 4487 Fragments arranged by size: 4487 1035 389 229 177 59 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2643 4107 5065 Size: 416 53 83 399 24 1226 1464 958 Cuts at: 5065 442 Size: 1753 Fragments arranged by size: 1753 1464 1226 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6376 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2524 2524 Size: 6376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2257 2654 2696 3374 5036 1239 Size: 1018 397 42 678 1662 2579 Fragments arranged by size: 2579 1662 1018 678 397 42 Bpu10I CC'TnA_GC Cuts at: 2534 2610 2534 Size: 76 6300 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2494 3581 3647 4501 5366 5664 2494 Size: 1087 66 854 865 298 3206 Fragments arranged by size: 3206 1087 865 854 298 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2081 5039 882 Size: 617 582 2958 2219 Fragments arranged by size: 2958 2219 617 582 BsaAI yAC'GTr Cuts at: 493 1204 4167 493 Size: 711 2963 2702 Fragments arranged by size: 2963 2702 711 BsaBI GATnn'nnATC Cuts at: 6370 6370 Size: 6376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 4183 4565 5200 Size: 53 83 186 936 486 2164 382 635 Cuts at: 5200 275 Size: 1451 Fragments arranged by size: 2164 1451 936 635 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3201 4809 5207 5481 5628 6039 3201 Size: 1608 398 274 147 411 3538 Fragments arranged by size: 3538 1608 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3125 3155 1178 Size: 30 165 30 1722 30 4399 Fragments arranged by size: 4399 1722 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2357 2360 2773 3083 1186 Size: 913 258 3 413 310 4479 Fragments arranged by size: 4479 913 413 310 258 3 BseYI C'CCAG_C Cuts at: 2216 2585 2690 2914 2944 5579 6075 2216 Size: 369 105 224 30 2635 496 2517 Fragments arranged by size: 2635 2517 496 369 224 105 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3037 1163 Size: 573 1301 4502 Fragments arranged by size: 4502 1301 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2443 3535 3826 4587 5615 1540 Size: 275 186 442 1092 291 761 1028 2301 Fragments arranged by size: 2301 1092 1028 761 442 291 275 186 BsiHKAI G_wGCw'C Cuts at: 2330 2352 2411 2684 3045 3235 3453 3480 3576 Size: 22 59 273 361 190 218 27 96 Cuts at: 3576 4437 4522 5593 2330 Size: 861 85 1071 3113 Fragments arranged by size: 3113 1071 861 361 273 218 190 96 85 59 27 22 BsmI GAATG_Cn' Cuts at: 3419 3887 3980 3419 Size: 468 93 5815 Fragments arranged by size: 5815 468 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 2352 2411 2533 2540 2592 2684 Size: 399 540 22 59 122 7 52 92 Cuts at: 2684 2691 2883 3045 3235 3453 3480 3576 3617 Size: 7 192 162 190 218 27 96 41 Cuts at: 3617 3695 4437 4522 5593 1391 Size: 78 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 540 399 218 192 190 162 122 96 92 85 78 59 52 41 27 22 7 7 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 4265 5995 4265 Size: 1730 4646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2995 3731 844 Size: 2151 736 3489 Fragments arranged by size: 3489 2151 736 BsrBI CCG'CTC Cuts at: 1394 1823 3097 3490 4263 1394 Size: 429 1274 393 773 3507 Fragments arranged by size: 3507 1274 773 429 393 BsrDI GCAATG_nn' Cuts at: 65 1248 4871 5045 65 Size: 1183 3623 174 1396 Fragments arranged by size: 3623 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3530 5020 5207 1700 Size: 110 1720 1490 187 2869 Fragments arranged by size: 2869 1720 1490 187 110 BsrGI T'GTAC_A Cuts at: 96 2467 96 Size: 2371 4005 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 3269 3485 4436 5448 3269 Size: 216 951 1012 4197 Fragments arranged by size: 4197 1012 951 216 BstAPI GCAn_nnn'nTGC Cuts at: 794 3060 794 Size: 2266 4110 BstBI TT'CG_AA Cuts at: 5180 5180 Size: 6376 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6376 BstXI CCAn_nnnn'nTGG Cuts at: 2800 2800 Size: 6376 BstYI r'GATC_y Cuts at: 1972 3077 3706 4073 4458 4475 5927 6013 6025 Size: 1105 629 367 385 17 1452 86 12 Cuts at: 6025 6371 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1105 629 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2304 2304 Size: 6376 BtgI C'CryG_G Cuts at: 513 1065 2004 3018 3549 513 Size: 552 939 1014 531 3340 Fragments arranged by size: 3340 1014 939 552 531 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2980 487 Size: 683 56 1754 3883 Fragments arranged by size: 3883 1754 683 56 BtsI GCAGTG_nn' Cuts at: 3058 3892 4686 4706 3058 Size: 834 794 20 4728 Fragments arranged by size: 4728 834 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6341 DraI TTT'AAA Cuts at: 3680 3680 Size: 6376 DraIII CAC_nnn'GTG Cuts at: 3239 3384 3239 Size: 145 6231 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5383 809 Size: 507 4067 1802 Fragments arranged by size: 4067 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3532 3552 3823 Size: 54 1425 50 275 18 1702 20 271 Cuts at: 3823 4712 6341 8 Size: 889 1629 43 Fragments arranged by size: 1702 1629 1425 889 275 271 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3532 3823 1537 Size: 275 1720 291 4090 Fragments arranged by size: 4090 1720 291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3143 3674 4306 1353 Size: 1790 531 632 3423 Fragments arranged by size: 3423 1790 632 531 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2045 4963 5337 5483 387 Size: 1064 138 456 2918 374 146 1280 Fragments arranged by size: 2918 1280 1064 456 374 146 138 EcoICRI GAG'CTC Cuts at: 2682 2682 Size: 6376 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2257 2654 2696 3374 4439 5036 5823 Size: 393 625 397 42 678 1065 597 787 Cuts at: 5823 6091 1239 Size: 268 1524 Fragments arranged by size: 1524 1065 787 678 625 597 397 393 268 42 EcoO109I rG'GnC_Cy Cuts at: 2173 2529 2530 2589 2629 2879 2880 2979 2173 Size: 356 1 59 40 250 1 99 5570 Fragments arranged by size: 5570 356 250 99 59 40 1 1 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3322 3634 3322 Size: 312 6064 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 5523 1678 Size: 3845 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2056 3062 3094 5017 Size: 32 135 32 452 32 1006 32 1923 Cuts at: 5017 5049 5091 5123 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1923 1006 452 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3720 3966 669 Size: 988 281 1782 246 3079 Fragments arranged by size: 3079 1782 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6376 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KpnI G_GTAC'C Cuts at: 3701 3701 Size: 6376 MfeI C'AATT_G Cuts at: 3975 3975 Size: 6376 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2061 2322 2805 5490 5674 1305 Size: 756 261 483 2685 184 2007 Fragments arranged by size: 2685 2007 756 483 261 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3554 10 Size: 54 1425 343 1722 2832 Fragments arranged by size: 2832 1722 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 2711 3325 3548 4334 4693 4852 Size: 648 1236 309 614 223 786 359 159 Cuts at: 4852 518 Size: 2042 Fragments arranged by size: 2042 1236 786 648 614 359 309 223 159 MspA1I CmG'CkG Cuts at: 1606 2220 2384 2902 3537 3792 4469 5617 5862 Size: 614 164 518 635 255 677 1148 245 Cuts at: 5862 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 635 614 518 255 245 164 NcoI C'CATG_G Cuts at: 513 1065 2004 3018 3549 513 Size: 552 939 1014 531 3340 Fragments arranged by size: 3340 1014 939 552 531 NdeI CA'TA_TG Cuts at: 387 387 Size: 6376 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6376 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 2914 3316 3734 5279 1132 Size: 97 1685 402 418 1545 2229 Fragments arranged by size: 2229 1685 1545 418 402 97 PasI CC'CwG_GG Cuts at: 1348 2416 2845 2873 1348 Size: 1068 429 28 4851 Fragments arranged by size: 4851 1068 429 28 PciI A'CATG_T Cuts at: 3312 5275 3312 Size: 1963 4413 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6376 PfoI T'CCnGG_A Cuts at: 3080 3174 3351 3080 Size: 94 177 6105 Fragments arranged by size: 6105 177 94 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3350 3382 4445 4477 5958 5990 3350 Size: 32 1063 32 1481 32 3736 Fragments arranged by size: 3736 1481 1063 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PspOMI G'GGCC_C Cuts at: 2529 2588 2879 2529 Size: 59 291 6026 Fragments arranged by size: 6026 291 59 PstI C_TGCA'G Cuts at: 830 2497 2928 3728 830 Size: 1667 431 800 3478 Fragments arranged by size: 3478 1667 800 431 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 PvuII CAG'CTG Cuts at: 2384 2902 2384 Size: 518 5858 SacI G_AGCT'C Cuts at: 2684 2684 Size: 6376 SalI G'TCGA_C Cuts at: 1936 3718 1936 Size: 1782 4594 SapI GCTCTTCn'nnn_ Cuts at: 3674 3674 Size: 6376 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SfcI C'TryA_G Cuts at: 826 1045 2356 2493 2924 3724 4859 5540 5731 Size: 219 1311 137 431 800 1135 681 191 Cuts at: 5731 826 Size: 1471 Fragments arranged by size: 1471 1311 1135 800 681 431 219 191 137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 2893 3344 3703 1872 Size: 1021 451 359 4545 Fragments arranged by size: 4545 1021 451 359 SmlI C'TyrA_G Cuts at: 820 1017 1943 2509 3596 3662 4480 5381 5643 Size: 197 926 566 1087 66 818 901 262 Cuts at: 5643 820 Size: 1553 Fragments arranged by size: 1553 1087 926 901 818 566 262 197 66 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 2914 3734 1132 Size: 1782 820 3774 Fragments arranged by size: 3774 1782 820 SrfI GCCC'GGGC Cuts at: 3344 3344 Size: 6376 SspI AAT'ATT Cuts at: 5 52 4300 5 Size: 47 4248 2081 Fragments arranged by size: 4248 2081 47 StuI AGG'CCT Cuts at: 1517 3287 1517 Size: 1770 4606 StyI C'CwwG_G Cuts at: 513 1065 2004 2571 3018 3549 3815 513 Size: 552 939 567 447 531 266 3074 Fragments arranged by size: 3074 939 567 552 531 447 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2327 2410 2771 3071 3337 4403 4420 Size: 1255 747 83 361 300 266 1066 17 Cuts at: 4420 4573 4758 5203 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1066 747 445 361 300 266 185 153 83 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2467 4622 6334 Size: 274 80 33 51 1443 490 2155 1712 Cuts at: 6334 96 Size: 138 Fragments arranged by size: 2155 1712 1443 490 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2133 2165 6336 6368 2133 Size: 32 4171 32 2141 Fragments arranged by size: 4171 2141 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6376 XbaI T'CTAG_A Cuts at: 3712 3712 Size: 6376 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2797 2797 Size: 6376 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 2891 3342 3701 1870 Size: 1021 451 359 4545 Fragments arranged by size: 4545 1021 451 359 XmnI GAAnn'nnTTC Cuts at: 3089 3656 4505 3089 Size: 567 849 4960 Fragments arranged by size: 4960 849 567 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SwaI