(Linear) (Six-base) MAPSORT of: KIEE2012.seq Check: 1725 from: 1 to: 1671 KIEE2012.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AccI GT'mk_AC Cuts at: 0 37 350 1671 Size: 37 313 1321 Fragments arranged by size: 1321 313 37 AflIII A'CryG_T Cuts at: 0 1307 1671 Size: 1307 364 AhdI GACnn_n'nnGTC Cuts at: 0 33 1671 Size: 33 1638 AleI CACnn'nnGTG Cuts at: 0 1320 1671 Size: 1320 351 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 814 846 1671 Size: 814 32 825 Fragments arranged by size: 825 814 32 ApaI G_GGCC'C Cuts at: 0 528 587 878 1671 Size: 528 59 291 793 Fragments arranged by size: 793 528 291 59 ApaLI G'TGCA_C Cuts at: 0 1226 1471 1671 Size: 1226 245 200 Fragments arranged by size: 1226 245 200 AvaI C'yCGr_G Cuts at: 0 326 886 1033 1337 1564 1671 Size: 326 560 147 304 227 107 Fragments arranged by size: 560 326 304 227 147 107 BamHI G'GATC_C Cuts at: 0 1072 1671 Size: 1072 599 BanI G'GyrC_C Cuts at: 0 1028 1205 1609 1671 Size: 1028 177 404 62 Fragments arranged by size: 1028 404 177 62 BanII G_rGCy'C Cuts at: 0 528 535 587 679 878 1671 Size: 528 7 52 92 199 793 Fragments arranged by size: 793 528 199 92 52 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 853 1027 1671 Size: 853 174 644 Fragments arranged by size: 853 644 174 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1422 1456 1671 Size: 1422 34 215 Fragments arranged by size: 1422 215 34 BglI GCCn_nnn'nGGC Cuts at: 0 22 1671 Size: 22 1649 BlpI GC'TnA_GC Cuts at: 0 380 1671 Size: 380 1291 Bme1580I G_kGCm'C Cuts at: 0 528 587 686 878 1230 1475 1612 1671 Size: 528 59 99 192 352 245 137 59 Fragments arranged by size: 528 352 245 192 137 99 59 59 BmgBI CAC'GTC Cuts at: 0 348 407 636 1025 1202 1671 Size: 348 59 229 389 177 469 Fragments arranged by size: 469 389 348 229 177 59 BmrI ACTGGGnnnn_n' Cuts at: 0 638 1671 Size: 638 1033 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 519 1671 Size: 519 1152 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 252 649 691 1369 1671 Size: 252 397 42 678 302 Fragments arranged by size: 678 397 302 252 42 Bpu10I CC'TnA_GC Cuts at: 0 529 605 1671 Size: 529 76 1066 Fragments arranged by size: 1066 529 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 489 1576 1642 1671 Size: 489 1087 66 29 Fragments arranged by size: 1087 489 66 29 BsaI GGTCTCn'nnnn_ Cuts at: 0 76 1671 Size: 76 1595 BsaHI Gr'CG_yC Cuts at: 0 14 1671 Size: 14 1657 BsaWI w'CCGG_w Cuts at: 0 1196 1671 Size: 1196 475 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1120 1150 1671 Size: 1120 30 521 Fragments arranged by size: 1120 521 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 94 352 355 768 1078 1671 Size: 94 258 3 413 310 593 Fragments arranged by size: 593 413 310 258 94 3 BseYI C'CCAG_C Cuts at: 0 211 580 685 909 939 1671 Size: 211 369 105 224 30 732 Fragments arranged by size: 732 369 224 211 105 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1032 1671 Size: 1032 639 BsiEI CG_ry'CG Cuts at: 0 438 1530 1671 Size: 438 1092 141 Fragments arranged by size: 1092 438 141 BsiHKAI G_wGCw'C Cuts at: 0 325 347 406 679 1040 1230 1448 1475 Size: 325 22 59 273 361 190 218 27 Cuts at: 1475 1571 1671 Size: 96 100 Fragments arranged by size: 361 325 273 218 190 100 96 59 27 22 BsmI GAATG_Cn' Cuts at: 0 1414 1671 Size: 1414 257 Bsp1286I G_dGCh'C Cuts at: 0 325 347 406 528 535 587 679 686 Size: 325 22 59 122 7 52 92 7 Cuts at: 686 878 1040 1230 1448 1475 1571 1612 1671 Size: 192 162 190 218 27 96 41 59 Fragments arranged by size: 325 218 192 190 162 122 96 92 59 59 52 41 27 22 7 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 990 1671 Size: 990 681 BsrBI CCG'CTC Cuts at: 0 1092 1485 1671 Size: 1092 393 186 Fragments arranged by size: 1092 393 186 BsrFI r'CCGG_y Cuts at: 0 1525 1671 Size: 1525 146 BsrGI T'GTAC_A Cuts at: 0 462 1671 Size: 462 1209 BssSI C'ACGA_G Cuts at: 0 1264 1480 1671 Size: 1264 216 191 Fragments arranged by size: 1264 216 191 BstAPI GCAn_nnn'nTGC Cuts at: 0 1055 1671 Size: 1055 616 BstXI CCAn_nnnn'nTGG Cuts at: 0 795 1671 Size: 795 876 BstYI r'GATC_y Cuts at: 0 1072 1671 Size: 1072 599 Bsu36I CC'TnA_GG Cuts at: 0 299 1671 Size: 299 1372 BtgI C'CryG_G Cuts at: 0 1013 1544 1671 Size: 1013 531 127 Fragments arranged by size: 1013 531 127 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 975 1671 Size: 975 696 BtsI GCAGTG_nn' Cuts at: 0 1053 1671 Size: 1053 618 DraIII CAC_nnn'GTG Cuts at: 0 1234 1379 1671 Size: 1234 145 292 Fragments arranged by size: 1234 292 145 EaeI y'GGCC_r Cuts at: 0 1527 1547 1671 Size: 1527 20 124 Fragments arranged by size: 1527 124 20 EagI C'GGCC_G Cuts at: 0 1527 1671 Size: 1527 144 EarI CTCTTCn'nnn_ Cuts at: 0 1138 1669 1671 Size: 1138 531 2 Fragments arranged by size: 1138 531 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 40 1671 Size: 40 1631 EcoICRI GAG'CTC Cuts at: 0 677 1671 Size: 677 994 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 252 649 691 1369 1671 Size: 252 397 42 678 302 Fragments arranged by size: 678 397 302 252 42 EcoO109I rG'GnC_Cy Cuts at: 0 168 524 525 584 624 874 875 974 Size: 168 356 1 59 40 250 1 99 Cuts at: 974 1671 Size: 697 Fragments arranged by size: 697 356 250 168 99 59 40 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1317 1629 1671 Size: 1317 312 42 Fragments arranged by size: 1317 312 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 19 51 1057 1089 1671 Size: 19 32 1006 32 582 Fragments arranged by size: 1006 582 32 32 19 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 56 317 800 1671 Size: 56 261 483 871 Fragments arranged by size: 871 483 261 56 MscI TGG'CCA Cuts at: 0 1549 1671 Size: 1549 122 MslI CAynn'nnrTG Cuts at: 0 397 706 1320 1543 1671 Size: 397 309 614 223 128 Fragments arranged by size: 614 397 309 223 128 MspA1I CmG'CkG Cuts at: 0 215 379 897 1532 1671 Size: 215 164 518 635 139 Fragments arranged by size: 635 518 215 164 139 NcoI C'CATG_G Cuts at: 0 1013 1544 1671 Size: 1013 531 127 Fragments arranged by size: 1013 531 127 NspI r_CATG'y Cuts at: 0 909 1311 1671 Size: 909 402 360 Fragments arranged by size: 909 402 360 PasI CC'CwG_GG Cuts at: 0 411 840 868 1671 Size: 411 429 28 803 Fragments arranged by size: 803 429 411 28 PciI A'CATG_T Cuts at: 0 1307 1671 Size: 1307 364 PfoI T'CCnGG_A Cuts at: 0 1075 1169 1346 1671 Size: 1075 94 177 325 Fragments arranged by size: 1075 325 177 94 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1345 1377 1671 Size: 1345 32 294 Fragments arranged by size: 1345 294 32 PspOMI G'GGCC_C Cuts at: 0 524 583 874 1671 Size: 524 59 291 797 Fragments arranged by size: 797 524 291 59 PstI C_TGCA'G Cuts at: 0 492 923 1671 Size: 492 431 748 Fragments arranged by size: 748 492 431 PvuII CAG'CTG Cuts at: 0 379 897 1671 Size: 379 518 774 Fragments arranged by size: 774 518 379 SacI G_AGCT'C Cuts at: 0 679 1671 Size: 679 992 SapI GCTCTTCn'nnn_ Cuts at: 0 1669 1671 Size: 1669 2 SfcI C'TryA_G Cuts at: 0 351 488 919 1671 Size: 351 137 431 752 Fragments arranged by size: 752 431 351 137 SmaI CCC'GGG Cuts at: 0 888 1339 1671 Size: 888 451 332 Fragments arranged by size: 888 451 332 SmlI C'TyrA_G Cuts at: 0 504 1591 1657 1671 Size: 504 1087 66 14 Fragments arranged by size: 1087 504 66 14 SphI G_CATG'C Cuts at: 0 909 1671 Size: 909 762 SrfI GCCC'GGGC Cuts at: 0 1339 1671 Size: 1339 332 StuI AGG'CCT Cuts at: 0 1282 1671 Size: 1282 389 StyI C'CwwG_G Cuts at: 0 566 1013 1544 1671 Size: 566 447 531 127 Fragments arranged by size: 566 531 447 127 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 322 405 766 1066 1332 1671 Size: 322 83 361 300 266 339 Fragments arranged by size: 361 339 322 300 266 83 TatI w'GTAC_w Cuts at: 0 462 1671 Size: 462 1209 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 128 160 1671 Size: 128 32 1511 Fragments arranged by size: 1511 128 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 792 1671 Size: 792 879 XmaI C'CCGG_G Cuts at: 0 886 1337 1671 Size: 886 451 334 Fragments arranged by size: 886 451 334 XmnI GAAnn'nnTTC Cuts at: 0 1084 1651 1671 Size: 1084 567 20 Fragments arranged by size: 1084 567 20 Enzymes that do cut: AccI AflIII AhdI AleI AloI ApaI ApaLI AvaI BamHI BanI BanII BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI Hin4I MmeI MscI MslI MspA1I NcoI NspI PasI PciI PfoI PpiI PspOMI PstI PvuII SacI SapI SfcI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AlfI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BglII BmtI BsaAI BsaBI BsiWI BsmBI BspEI BspHI BsrDI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI ZraI