Restriction map for
pFN21AE0186
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BmyI BpmI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvaI MvnI NaeI NarI
NciI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI PvuII
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TspRI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AclI 38 AACGTT AA^CGTT
AvaII 427 GGACC G^G[AT]CC
BbsI 203 GTCTTC GAAGAC(2/6)
BfaI 175 CTAG C^TAG
BlpI 576 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 576 GCTGAGC GC^T[ACGT]AGC
BpuAI 203 GTCTTC GAAGAC(2/6)
BseRI 214 GAGGAG GAGGAG(10/8)
BsgI 47 GTGCAG GTGCAG(16/14)
BsmBI 635 CGTCTC CGTCTC(1/5)
BsrI 592 CCAGT ACTGG(1/-1)
Cac8I 343 GCAAGC GC[ACGT]^[ACGT]GC
CelII 576 GCTGAGC GC^T[ACGT]AGC
DrdI 194 GACAGACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeIII 590 GGCC GG^CC
HgaI 268 GCGTC GACGC(5/10)
HincII 556 GTCAAC GT[CT]^[GA]AC
HindII 556 GTCAAC GT[CT]^[GA]AC
HindIII 345 AAGCTT A^AGCTT
HphI 79 GGTGA GGTGA(8/7)
MaeI 175 CTAG C^TAG
MaeIII 168 GTCAC ^GT[ACGT]AC
MwoI 122 GCTACATGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 381 CATATG CA^TATG
NlaIV 426 GGGACC GG[ACGT]^[ACGT]CC
PleI 516 GACTC GAGTC(4/5)
Psp1406I 38 AACGTT AA^CGTT
PstI 260 CTGCAG CTGCA^G
TaqI 224 TCGA T^CGA
TfiI 606 GAATC G^A[AT]TC
Tsp45I 168 GTCAC ^GT[GC]AC
TthHB8I 224 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 328 GTAC GT^AC
484 GTAC
AflIII 125 ACATGT A^C[GA][CT]GT
199 ACATGT
Asp700I 388 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
504 GAATGAATTC
BbvI 262 GCAGC GCAGC(8/12)
265 GCAGC
BsaI 96 GAGACC GGTCTC(1/5)
360 GAGACC
BsiYI 460 CCAATACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
592 CCAGTTGTTGG
BslI 460 CCAATACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
592 CCAGTTGTTGG
BspHI 396 TCATGA T^CATGA
512 TCATGA
BspLU11I 125 ACATGT A^CATGT
199 ACATGT
Cfr13I 427 GGACC G^G[ACGT]CC
589 GGGCC
Fnu4HI 262 GCAGC GC^[ACGT]GC
265 GCAGC
HinfI 516 GACTC G^A[ACGT]TC
606 GAATC
ItaI 262 GCAGC GC^[ACGT]GC
265 GCAGC
MaeII 39 ACGT A^CGT
634 ACGT
NspI 125 ACATGT [GA]CATG^[CT]
199 ACATGT
PflMI 460 CCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
592 CCAGTTGTTGG
RcaI 396 TCATGA T^CATGA
512 TCATGA
RsaI 328 GTAC GT^AC
484 GTAC
Sau96I 427 GGACC G^G[ACGT]CC
589 GGGCC
SfaNI 411 GATGC GCATC(5/9)
181 GCATC
TseI 262 GCAGC G^C[AT]GC
265 GCAGC
Van91I 460 CCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
592 CCAGTTGTTGG
XmnI 388 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
504 GAATGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BsmAI
BsmFI BstF5I DdeI DsaI EcoRI
EcoT14I FokI MboII MnlI MseI
NcoI NlaIII SfcI StyI Tru9I
Tsp509I