Restriction map for pFN21AE0171
 (Circular) (Six-base) MAPSORT of: pFN21AE0171.seq  Check: 8453  from: 1  to: 5371



pFN21AE0171  5371 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:45  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3181     278 
   Size:         53      83     186     936    1645    2468

  Fragments arranged by size: 

                2468    1645     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2714    1937 
   Size:        777    4594
 
Acc65I G'GTAC_C

Cuts at:   2692    2692 
   Size:       5371
 
AclI AA'CG_TT

Cuts at:   1563    3498    3871    1563 
   Size:       1935     373    3063

  Fragments arranged by size: 

                3063    1935     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2240    2288    2561    3434    4818    5086    1632 
   Size:        608      48     273     873    1384     268    1917

  Fragments arranged by size: 

                1917    1384     873     608     273     268      48
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5174
 
AflIII A'CryG_T

Cuts at:   1203    4270    5247    1203 
   Size:       3067     977    1327

  Fragments arranged by size: 

                3067    1327     977
 
AgeI A'CCGG_T

Cuts at:   4202    4202 
   Size:       5371
 
AhdI GACnn_n'nnGTC

Cuts at:   4100    4100 
   Size:       5371
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5371
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5371
 
AlwNI CAG_nnn'CTG

Cuts at:   3114    4686    3114 
   Size:       1572    3799
 
ApaLI G'TGCA_C

Cuts at:   3428    4584    3428 
   Size:       1156    4215
 
ApoI r'AATT_y

Cuts at:   1493    2680    2906    3058    5167    1493 
   Size:       1187     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1187     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3925     160 
   Size:        872    2893    1606

  Fragments arranged by size: 

                2893    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5371
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2696    1870 
   Size:         73     753    4545

  Fragments arranged by size: 

                4545     753      73
 
BamHI G'GATC_C

Cuts at:   2506    2701    3068    2506 
   Size:        195     367    4809

  Fragments arranged by size: 

                4809     367     195
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2027    2111    2692    4147     618 
   Size:        325     217     627     240      84     581    1455    1842

  Fragments arranged by size: 

                1842    1455     627     581     325     240     217      84
            
 
BanII G_rGCy'C

Cuts at:   1391    2069    2690    1391 
   Size:        678     621    4072

  Fragments arranged by size: 

                4072     678     621
 
BbeI G_GCGC'C

Cuts at:   2115    2115 
   Size:       5371
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4400
 
BbvCI CC'TCA_GC

Cuts at:   2318    2318 
   Size:       5371
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3562    3596    1903 
   Size:         34    1625      34    3678

  Fragments arranged by size: 

                3678    1625      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3263    4473    3263 
   Size:       1210    4161
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5371
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3982     136 
   Size:        107     122      71     631     605    2310    1525

  Fragments arranged by size: 

                2310    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5366    5366 
   Size:       5371
 
BlpI GC'TnA_GC

Cuts at:   2788    2788 
   Size:       5371
 
Bme1580I G_kGCm'C

Cuts at:   1790    3432    4588    1790 
   Size:       1642    1156    2573

  Fragments arranged by size: 

                2573    1642    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5371
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2641    3102    4060 
   Size:        416      53      83     399      24    1224     461     958

Cuts at:   4060     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1224     958     461     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5371
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2053    4031    1239 
   Size:        814    1978    2579

  Fragments arranged by size: 

                2579    1978     814
 
Bpu10I CC'TnA_GC

Cuts at:   2318    2437    2318 
   Size:        119    5252
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3496    4361    4659    3496 
   Size:        865     298    4208

  Fragments arranged by size: 

                4208     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4034     882 
   Size:        617    2535    2219

  Fragments arranged by size: 

                2535    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2179    3162     493 
   Size:        711     975     983    2702

  Fragments arranged by size: 

                2702     983     975     711
 
BsaBI GATnn'nnATC

Cuts at:   5365    5365 
   Size:       5371
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2112    3178    3560    4195 
   Size:         53      83     186     936     579    1066     382     635

Cuts at:   4195     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1066     936     635     579     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2047    3804    4202    4476    4623    5034    2047 
   Size:       1757     398     274     147     411    2384

  Fragments arranged by size: 

                2384    1757     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5146

  Fragments arranged by size: 

                5146     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2216    1186 
   Size:       1030    4341
 
BseYI C'CCAG_C

Cuts at:   2542    4574    5070    2542 
   Size:       2032     496    2843

  Fragments arranged by size: 

                2843    2032     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2135    2247    1163 
   Size:        573     399     112    4287

  Fragments arranged by size: 

                4287     573     399     112
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2821    3582    4610    1540 
   Size:        275     186     820     761    1028    2301

  Fragments arranged by size: 

                2301    1028     820     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2565    3432    3517    4588    2565 
   Size:        867      85    1071    3348

  Fragments arranged by size: 

                3348    1071     867      85
 
BsmI GAATG_Cn'

Cuts at:   2882    2975    2882 
   Size:         93    5278
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2069    2565    2690    3432    3517    4588    1391 
   Size:        399     279     496     125     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     496     399     279     125      85
            
 
BspEI T'CCGG_A

Cuts at:   5034    5034 
   Size:       5371
 
BspHI T'CATG_A

Cuts at:   3260    4990    3260 
   Size:       1730    3641
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2103    2726     844 
   Size:       1259     623    3489

  Fragments arranged by size: 

                3489    1259     623
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3258    1394 
   Size:        429    1435    3507

  Fragments arranged by size: 

                3507    1435     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3866    4040      65 
   Size:       1183    2618     174    1396

  Fragments arranged by size: 

                2618    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4015    4202    1700 
   Size:        110    2205     187    2869

  Fragments arranged by size: 

                2869    2205     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5371
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5371
 
BssSI C'ACGA_G

Cuts at:   3431    4443    3431 
   Size:       1012    4359
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2143     794 
   Size:       1349    4022
 
BstBI TT'CG_AA

Cuts at:   4175    4175 
   Size:       5371
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5371
 
BstYI r'GATC_y

Cuts at:   1972    2506    2701    3068    3453    3470    4922    5008    5020 
   Size:        534     195     367     385      17    1452      86      12

Cuts at:   5020    5366    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     534     385     367     346     195      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4819
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4632

  Fragments arranged by size: 

                4632     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2025    2510    2537    2887    3681    3701    2025 
   Size:        485      27     350     794      20    3695

  Fragments arranged by size: 

                3695     794     485     350      27      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5371
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2263    2298     529 
   Size:         35    1699      35    3602

  Fragments arranged by size: 

                3602    1699      35      35
 
DraI TTT'AAA

Cuts at:   2675    2675 
   Size:       5371
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4378     809 
   Size:        507    3062    1802

  Fragments arranged by size: 

                3062    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2223    2818    3707 
   Size:         54    1425      50     275      18     393     595     889

Cuts at:   3707    5336       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     595     393     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2818    1537 
   Size:        275    1006    4090

  Fragments arranged by size: 

                4090    1006     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2259    3301    1353 
   Size:        906    1042    3423

  Fragments arranged by size: 

                3423    1042     906
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3958    4332    4478     387 
   Size:       1064     138    2369     374     146    1280

  Fragments arranged by size: 

                2369    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2053    2240    2288    2561    3434    4031    4818 
   Size:        393     421     187      48     273     873     597     787

Cuts at:   4818    5086    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     873     787     597     421     393     273     268
                 187      48
 
EcoRI G'AATT_C

Cuts at:   2680    3058    2680 
   Size:        378    4993
 
FspI TGC'GCA

Cuts at:   3877    3877 
   Size:       5371
 
HaeII r_GCGC'y

Cuts at:   1678    2115    4518    1678 
   Size:        437    2403    2531

  Fragments arranged by size: 

                2531    2403     437
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4012    4044    4086    4118    1373 
   Size:         32     135      32    2440      32      42      32    2626

  Fragments arranged by size: 

                2626    2440     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2715    2961     669 
   Size:        988     281     777     246    3079

  Fragments arranged by size: 

                3079     988     777     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5371
 
HpaI GTT'AAC

Cuts at:   2961    2961 
   Size:       5371
 
KasI G'GCGC_C

Cuts at:   2111    2111 
   Size:       5371
 
KpnI G_GTAC'C

Cuts at:   2696    2696 
   Size:       5371
 
MfeI C'AATT_G

Cuts at:   2970    2970 
   Size:       5371
 
MluI A'CGCG_T

Cuts at:   5247    5247 
   Size:       5371
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4485    4669    1305 
   Size:       3180     184    2007

  Fragments arranged by size: 

                3180    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2225      10 
   Size:         54    1425     343     393    3156

  Fragments arranged by size: 

                3156    1425     393     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3329    3688    3847     518 
   Size:        648    2163     359     159    2042

  Fragments arranged by size: 

                2163    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2041    2149    2787    3464    4612    4857    1606 
   Size:        435     108     638     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     638     435     245     108
 
NarI GG'CG_CC

Cuts at:   2112    2112 
   Size:       5371
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4819
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5371
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5371
 
NotI GC'GGCC_GC

Cuts at:   2818    2818 
   Size:       5371
 
NspI r_CATG'y

Cuts at:   1132    1229    2729    4274    1132 
   Size:         97    1500    1545    2229

  Fragments arranged by size: 

                2229    1545    1500      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5371
 
PciI A'CATG_T

Cuts at:   4270    4270 
   Size:       5371
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5371
 
PmeI GTTT'AAAC

Cuts at:   2675    2675 
   Size:       5371
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3440    3472    4953    4985    3440 
   Size:         32    1481      32    3826

  Fragments arranged by size: 

                3826    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5371
 
PsiI TTA'TAA

Cuts at:   2941    2941 
   Size:       5371
 
PstI C_TGCA'G

Cuts at:    830    2723     830 
   Size:       1893    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5371
 
PvuII CAG'CTG

Cuts at:   2041    2149    2041 
   Size:        108    5263
 
SalI G'TCGA_C

Cuts at:   1936    2713    1936 
   Size:        777    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2259    2259 
   Size:       5371
 
SbfI CC_TGCA'GG

Cuts at:   2723    2723 
   Size:       5371
 
ScaI AGT'ACT

Cuts at:   3619    5331    3619 
   Size:       1712    3659
 
SfcI C'TryA_G

Cuts at:    826    1045    2719    3854    4535    4726     826 
   Size:        219    1674    1135     681     191    1471

  Fragments arranged by size: 

                1674    1471    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2113    2113 
   Size:       5371
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5371
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5371
 
SmaI CCC'GGG

Cuts at:   1872    2698    1872 
   Size:        826    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3475    4376    4638     820 
   Size:        197     926    1532     901     262    1553

  Fragments arranged by size: 

                1553    1532     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3162     493 
   Size:       2669    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5371
 
SphI G_CATG'C

Cuts at:   1132    2729    1132 
   Size:       1597    3774
 
SspI AAT'ATT

Cuts at:      5      52    3295       5 
   Size:         47    3243    2081

  Fragments arranged by size: 

                3243    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2529    2596    1517 
   Size:       1012      67    4292

  Fragments arranged by size: 

                4292    1012      67
 
StyI C'CwwG_G

Cuts at:    513    1065    2226    2431    2810     513 
   Size:        552    1161     205     379    3074

  Fragments arranged by size: 

                3074    1161     552     379     205
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3398    3415    3568    3753    4198     325 
   Size:       1255    1818      17     153     185     445    1498

  Fragments arranged by size: 

                1818    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3617    5329      96 
   Size:        274      80      33      51    1443    1640    1712     138

  Fragments arranged by size: 

                1712    1640    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5331    5363    5331 
   Size:         32    5339
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5371
 
XbaI T'CTAG_A

Cuts at:   2707    2707 
   Size:       5371
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2038    2038 
   Size:       5371
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5371
 
XmaI C'CCGG_G

Cuts at:   1870    2696    1870 
   Size:        826    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3500    3500 
   Size:       5371
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3179     276 
   Size:         53      83     186     936    1645    2468

  Fragments arranged by size: 

                2468    1645     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BfrBI
     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     NaeI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SrfI
     SwaI
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