Restriction map for
pFN21AE1842
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# NO CUT ENZYMES ( 128 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AvrII BalI
BamHI BbrPI BbsI BbvCI BciVI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI Bst1107I BstBI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT22I HincII
HindII HindIII HpaI KpnI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NdeI
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AatII 177 GACGTC GACGT^C
AscI 328 GGCGCGCC GG^CGCGCC
BcgI 872 GCAACAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 476 TGATCA T^GATCA
BlpI 990 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 990 GCTGAGC GC^T[ACGT]AGC
BseRI 496 GAGGAG GAGGAG(10/8)
BssSI 172 CACGAG CACGAG(-5/-1)
BstAPI 933 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 667 GGTCACC G^GT[ACGT]ACC
BstF5I 501 GGATG GGATG(2/0)
BstPI 667 GGTCACC G^GT[ACGT]ACC
BstXI 509 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 990 GCTGAGC GC^T[ACGT]AGC
DdeI 991 CTGAG C^T[ACGT]AG
EarI 532 CTCTTC CTCTTC(1/4)
EcoO65I 667 GGTCACC G^GT[ACGT]ACC
FbaI 476 TGATCA T^GATCA
FokI 501 GGATG GGATG(9/13)
FseI 525 GGCCGGCC GGCCGG^CC
HinfI 4 GAGTC G^A[ACGT]TC
Ksp632I 532 CTCTTC CTCTTC(1/4)
NcoI 1058 CCATGG C^CATGG
PleI 4 GAGTC GAGTC(4/5)
SacI 1041 GAGCTC GAGCT^C
SgrAI 455 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 757 CTCAAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 438 GTAC GT^AC
852 GTAC
AspHI 169 GAGCAC G[AT]GC[AT]^C
1041 GAGCTC
AviII 887 TGCGCA TGC^GCA
971 TGCGCA
BpmI 427 CTGGAG CTGGAG(16/14)
943 CTGGAG
BsiHKAI 169 GAGCAC G[AT]GC[AT]^C
1041 GAGCTC
BsmAI 175 GAGAC GTCTC(1/5)
925 GAGAC
BsmBI 175 GAGACG CGTCTC(1/5)
925 GAGACG
BsrI 1002 ACTGG ACTGG(1/-1)
953 CCAGT
BssHII 329 GCGCGC G^CGCGC
752 GCGCGC
DpnII 477 GATC ^GATC
627 GATC
FspI 887 TGCGCA TGC^GCA
971 TGCGCA
HgaI 927 GACGC GACGC(5/10)
446 GCGTC
HphI 474 GGTGA GGTGA(8/7)
669 TCACC
KspI 31 CCGCGG CCGC^GG
335 CCGCGG
MaeII 178 ACGT A^CGT
920 ACGT
MaeIII 668 GTCAC ^GT[ACGT]AC
956 GTGAC
MboI 477 GATC ^GATC
627 GATC
MboII 533 TCTTC GAAGA(8/7)
1022 TCTTC
MslI 687 CACTCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
879 CATCGCGGTG
NdeII 477 GATC ^GATC
627 GATC
NotI 400 GCGGCCGC GC^GGCCGC
465 GCGGCCGC
RsaI 438 GTAC GT^AC
852 GTAC
SacII 31 CCGCGG CCGC^GG
335 CCGCGG
Sau3AI 477 GATC ^GATC
627 GATC
SfaNI 161 GCATC GCATC(5/9)
1019 GCATC
SfiI 270 GGCCAAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
733 GGCCTGCCGGGCC
TaqI 185 TCGA T^CGA
203 TCGA
Tsp45I 668 GTCAC ^GT[GC]AC
956 GTGAC
TspRI 634 CACTG CA[GC]TG[ACGT][ACGT]^
954 CAGTG
TthHB8I 185 TCGA T^CGA
203 TCGA
XcmI 272 CCAAGGCGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
821 CCAAGAAGTCGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI BanI BanII BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiEI BsiYI BslI BsoBI Bsp1286I
BspMI BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NgoMIV NlaIII
NlaIV PstI PvuII Sau96I ScrFI
SfcI SfoI SmaI StyI TseI
XmaCI XmaI